LOCUS       40924_7391        8184 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBSD-GFP-INT-attP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8184)
  AUTHORS   Nkrumah LJ, Muhle RA, Moura PA, Ghosh P, Hatfull GF, Jacobs WR Jr., 
            Fidock DA.
  TITLE     Efficient site-specific integration in Plasmodium falciparum 
            chromosomes mediated by mycobacteriophage Bxb1 integrase
  JOURNAL   Nat. Methods 3 (8), 615-621 (2006)
  PUBMED    16862136
REFERENCE   2  (bases 1 to 8184)
  AUTHORS   Nkrumah LJ, Moura PA, Fidock DA.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2006) Microbiology and Immunology, Albert Einstein
            College of Medicine, 1300 Morris Park, Bronx, NY 10461, USA
REFERENCE   3  (bases 1 to 8184)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8184)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Methods"; date: "2006"; volume: "3"; issue: "8"; pages: "615-621"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-JUN-2006) Microbiology and Immunology, Albert Einstein College 
            of Medicine, 1300 Morris Park, Bronx, NY 10461, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      complement(37..865)
                     /label=derived from hsp86 3' UTR
                     /note="derived from hsp86 3' UTR"
                     /regulatory_class="terminator"
     CDS             complement(875..1585)
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     CDS             complement(1592..1615)
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     misc_feature    complement(1622..2020)
                     /gene="bsd-FLAG-gfp"
                     /label=BSD
                     /note="BSD"
     CDS             complement(1622..2020)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MHAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFT
                     GVNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPG
                     IKAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    complement(2036..2659)
                     /label=contains promoter and 5' UTR from calmodulin gene
                     /note="contains promoter and 5' UTR from calmodulin gene"
     misc_feature    2666..3264
                     /label=contains promoter and 5' UTR from PcDT gene
                     /note="contains promoter and 5' UTR from PcDT gene"
     CDS             4799..4828
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     regulatory      4838..5428
                     /label=derived from hrp2 3' UTR
                     /note="derived from hrp2 3' UTR"
                     /regulatory_class="terminator"
     misc_recomb     5447..5496
                     /label=attP
                     /note="attP"
     promoter        complement(5502..5519)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5526..5542)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6016..6120
                     /label=AmpR promoter
     CDS             6121..6978
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      7152..7740
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    8028..8049
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8064..8094
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8102..8118
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8126..8142
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8160..8178
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatcctatt tgatgaatta actacactta
       61 aaataataca attattatta aatttttttt tgatttattt attaattttt aaacttaatc
      121 atttgtattt gggaggaatt atatatatct ttataattat tttatttttt tttatttttt
      181 tattttttta ttattattat ttttttttat tttttttttt tactgtatca aagaaaaacc
      241 tttaaaaaaa aaattataat ttccccatct tactatattt ttaatacata cgttttaagg
      301 aattaaatta gacaaaagct atattatgct ttacatataa ttagaattta taaacgtttg
      361 gttattagat atttcatgtc tcagtaaagt ctttcaatac atatgtaaaa aaatatatat
      421 gaatacacat aagttgttaa tatattttat atgcataaat gtataaatat atatatatat
      481 atatatatat gtatgtatgt atatgtgtgt atatgaaatt atttcaatgt ttaatttttt
      541 aaattttaat tttttttttt tttttttttt ttattatgta tattgatctt tattatttaa
      601 atattacttt tttcgttttt tcttcttttt attatttttt ttttttttta tattttatac
      661 aaatggtaat tcaaataaaa ggtataaatt tatatttaat tttcttttat ggataaataa
      721 aagaaaaata taaatatata aaaatataaa aatatatata tgtatattgg ggtgatgata
      781 aaatgaaaga taatatatat atatatatat ctttattttt ttttttttgt agaccccatt
      841 gtgagtacat aaatatatta tataactcga gttatttgta tagttcatcc atgccatgtg
      901 taatcccagc agctgttaca aactcaagaa ggaccatgtg gtctctcttt tcgttgggat
      961 ctttcgaaag ggcagattgt gtggacaggt aatggttgtc tggtaaaagg acagggccat
     1021 cgccaattgg agtattttgt tgataatggt ctgctagttg aacgcttcca tcttcaatgt
     1081 tgtgtctaat tttgaagtta actttgattc cattcttttg tttgtctgcc atgatgtata
     1141 cattgtgtga gttatagttg tattccaatt tgtgtccaag aatgtttcca tcttctttaa
     1201 aatcaatacc ttttaactcg attctattaa caagggtatc accttcaaac ttgacttcag
     1261 cacgtgtctt gtagttcccg tcatctttga aaaatatagt tctttcctgt acataacctt
     1321 cgggcatggc actcttgaaa aagtcatgct gtttcatatg atctgggtat ctcgcaaagc
     1381 attgaagacc atacgcgaaa gtagtgacaa gtgttggcca tggaacaggt agttttccag
     1441 tagtgcaaat aaatttaagg gtaagttttc cgtatgttgc atcaccttca ccctctccac
     1501 tgacagaaaa tttgtgccca ttaacatcac catctaattc aacaagaatt gggacaactc
     1561 cagtgaaaag ttcttctcct ttactagatc tcttgtcatc gtcgtccttg tagtcactag
     1621 tgccctccca cacataacca gagggcagca attcacgaat cccaactgcc gtcggctgtc
     1681 catcactgtc cttcactatg gctttgatcc caggatgcag atcgagaagc acctgtcggc
     1741 accgtccgca ggggctcaag atgcccctgt tctcatttcc gatcgcgacg atacaagtca
     1801 ggttgccagc tgccgcagca gcagcagtgc ccagcaccac gagttctgca caaggtcccc
     1861 cagtaaaatg atatacattg acaccagtga agatgcggcc gtcgctagag agagctgcgc
     1921 tggcgacgct gtagtcttca gagatgggga tgctgttgat tgtagccgtt gctctttcaa
     1981 tgagggtgga ttcttcttga gacaaaggct tggcatgcat tttctcgagg gatcctgata
     2041 tatttctatt aggtatttat tattataaaa tataaatctt gaatgataat aaataaaata
     2101 ttagttattc cttttctagt ttaaaatata catattataa atatatatat atatatatat
     2161 atttttattg tgacaagaat atataattat aaattatatt atttattttt gtattttttt
     2221 tttttttttt ttttttttct ttttttgttt tatttttctt tttttttata aatattattt
     2281 ttttctttta tcatgcacat tggaataata cattaatata tatatatata ttatattata
     2341 catatattga ataatgttta taaaaaatgc ataacttata tgaatataat tttttttaaa
     2401 tatgacaaaa agaaaaaaaa aaaaaaccaa aaaaaattaa aattgaaatg aaatatataa
     2461 atatattatt tatatatatt atacattgtt taatactact acatgtatat atatatatta
     2521 tatatatata tatatatcaa ttttttcaaa aataaattaa tataaaaaga ggggaaaaaa
     2581 aaaaaaaaaa aaaaaaaaag ataattaagt aagcatttaa aaatatataa attgataata
     2641 tataaaatta atcacatata agcttgcatg cttagctaat tcgcttgtaa gaggtactct
     2701 cgtttatgca aaactatttg atatagcatt ttaacaagta cacatatata tatgtaatat
     2761 atatactata tatatctatt gcatgtgtac taagcatgtg catggcatcc cctttttctc
     2821 gtgtttaaaa cagtttgtat gataaaatat aaaggatttg aaaaagagaa aaaaatatat
     2881 gatctcatcc tatatagcgc cataattttt atttgggttg aataaaattt tctactaaat
     2941 ttaggtgtaa gtaaaataat ggaatatata taagtacaat aaaaaagtgc ataaattaaa
     3001 aaatttttat aataaatatt ttttttaaaa aagtcaataa taatattaaa tatatataac
     3061 acaggattat atatgttcac tacaattttt tatattataa tataaattct tttcaatttt
     3121 cattttattt tacatacact ttcctttttt gtcactatat tttaatattc acatatttag
     3181 tttaaatact ggctatttct ttctacattt gctagtaaca attgtgtagt gcttatatat
     3241 atacacacac ctaaaactta caaaccatgg tgataacaaa atgagagccc tggtagtcat
     3301 ccgcctgtcc cgcgtcaccg atgctacgac ttcaccggag cgtcagctgg agtcttgcca
     3361 gcagctctgc gcccagcgcg gctgggacgt cgtcggggta gcggaggatc tggacgtctc
     3421 cggggcggtc gatccgttcg accggaagcg cagaccgaac ctggcccggt ggctagcgtt
     3481 cgaggagcaa ccgttcgacg tgatcgtggc gtaccgggta gaccggttga cccgatcgat
     3541 ccggcatctg cagcagctgg tccactgggc cgaggaccac aagaagctgg tcgtctccgc
     3601 gaccgaagcg cacttcgata cgacgacgcc gtttgcggcg gtcgtcatcg cgcttatggg
     3661 aacggtggcg cagatggaat tagaagcgat caaagagcgg aaccgttcgg ctgcgcattt
     3721 caatatccgc gccgggaaat accgaggatc cctgccgccg tggggatacc tgcctacgcg
     3781 cgtggacggg gagtggcggc tggtgccgga ccctgtgcag cgagagcgca tcctcgaggt
     3841 gtatcaccgc gtcgtcgaca accacgagcc gctgcacctg gtggcccacg acctgaaccg
     3901 gcgtggtgtc ctgtcgccga aggactactt cgcgcagctg caaggccgcg agccgcaggg
     3961 ccgggagtgg tcggctaccg cgctgaagcg atcgatgatc tccgaggcga tgctcgggta
     4021 cgcgactctg aacggtaaga ccgtccgaga cgacgacgga gccccgctgg tgcgggctga
     4081 gccgatcctg acccgtgagc agctggaggc gctgcgcgcc gagctcgtga agacctcccg
     4141 ggcgaagccc gcggtgtcta ccccgtcgct gctgctgcgg gtgttgttct gcgcggtgtg
     4201 cggggagccc gcgtacaagt tcgccggggg aggacgtaag cacccgcgct accgctgccg
     4261 ctcgatgggg ttcccgaagc actgcgggaa cggcacggtg gcgatggccg agtgggacgc
     4321 gttctgcgag gagcaggtgc tggatctgct cggggacgcg gagcgtctgg agaaagtctg
     4381 ggtagccggc tcggactccg cggtcgaact cgcggaggtg aacgcggagc tggtggacct
     4441 gacgtcgctg atcggctccc cggcctaccg ggccggctct ccgcagcgag aagcactgga
     4501 tgcccgtatt gcggcgctgg ccgcgcggca agaggagctg gagggtctag aggctcgccc
     4561 gtctggctgg gagtggcgcg agaccgggca gcggttcggg gactggtggc gggagcagga
     4621 caccgcggca aagaacacct ggcttcggtc gatgaacgtt cggctgacgt tcgacgtccg
     4681 cggcgggctg actcgcacga tcgacttcgg ggatctgcag gagtacgagc agcatctcag
     4741 gctcggcagc gtggtcgaac ggctacacac cgggatgtcg agagcggccg cactcgagga
     4801 acaaaagcta ataagtgaag aagatttata aggtacccca ttaaatttat ttaataatag
     4861 attaaaaata ttataaaaat aaaaacataa acacagaaat tacaaaaaaa atacatatga
     4921 attttttttt tgtaatcttc cttataaata tagaataatg aatcatataa aacatatcat
     4981 ttttcattta tttacattta aaattattgt ttcagtatct ttaatttatt atgtatatat
     5041 aaaaataact tacaatttta ttaataaaca atatatgttt attaattcat gttttgtaat
     5101 ttatgggata gcgatttttt ttactgtctg tatttttctt ttttaattat gttttaattg
     5161 tattttattt ttattattgt tctttttata gtattatttt aaaacaaaat gttttttcta
     5221 agaacttata ataataataa atataaattt taataaaaat tatatttatc ttttacaata
     5281 tgaacataaa gtacaacatt aatatatagc ttttaatatt tttattccta atcatgtaaa
     5341 tcttaaattt ttctttttaa acatatgtta aatatttatt tctcattata tataaggaca
     5401 tatttattaa accgcagaga aatctagagg taccgagctc gaattcggtt tgtctggtca
     5461 accaccgcgg tctcagtggt gtacggtaca aaccccgaat tctatagtga gtcgtattac
     5521 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     5581 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     5641 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt
     5701 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
     5761 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     5821 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     5881 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     5941 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     6001 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     6061 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     6121 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     6181 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     6241 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     6301 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     6361 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     6421 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     6481 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     6541 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     6601 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     6661 cctgtagcaa tgccaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     6721 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     6781 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     6841 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     6901 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     6961 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     7021 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     7081 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     7141 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     7201 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     7261 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     7321 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     7381 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     7441 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     7501 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     7561 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     7621 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     7681 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatcgaaa
     7741 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     7801 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     7861 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     7921 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     7981 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     8041 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     8101 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcta
     8161 tttaggtgac actatagaat actc
//
