LOCUS       62056_4185        5330 bp DNA     circular SYN 12-JUN-2019
DEFINITION  Transformation vector pBS-PtPDS1-M1, complete sequence.
ACCESSION   MK645853
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Taparia Y, Zarka A, Leu S, Zarivach R, Boussiba S, Khozin-Goldberg 
            I.
  TITLE     A novel endogenous selection marker for the diatom Phaeodactylum 
            tricornutum based on a unique mutation in phytoene desaturase 1
  JOURNAL   Sci Rep 9 (1), 8217 (2019)
  PUBMED    31160749
REFERENCE   2  (bases 1 to 5330)
  AUTHORS   Taparia Y, Leu S, Zarka A, Zarivach R, Khozin-Goldberg I, Boussiba 
            S.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2019) Albert Katz International School for Desert 
            Research, Ben-Gurion University of The Negev, French Associates 
            Institute for Agriculture and Biotechnology, Sede Boqer, Israel 
            8499000, Israel
REFERENCE   3  (bases 1 to 5330)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep"; 
            date: "2019"; volume: "9"; issue: "1"; pages: "8217"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-MAR-2019) Albert Katz International School for Desert Research, 
            Ben-Gurion University of The Negev, French Associates Institute for 
            Agriculture and Biotechnology, Sede Boqer, Israel 8499000, Israel"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     670..686
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      731..976
                     /regulatory_class="promoter"
     CDS             1243..1257
                     /label=SNAC tag
                     /note="tag for protein cleavage using Ni2+ ion (Dang et
                     al., 2019)"
     regulatory      2852..3093
                     /regulatory_class="terminator"
     promoter        complement(3142..3160)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3181..3197)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3205..3221)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3229..3259)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3274..3295)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3583..4171)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4345..5202)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5203..5307)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccggggg
      721 atccactagt acataccttc agcgtcgtct tcactgtcac agtcaactga cagtaatcgt
      781 tgatccggag agattcaaaa ttcaatctgt ttggacctgg ataagacaca agagcgacat
      841 cctgacatga acgccgtaaa cagcaaatcc tggttgaaca cgtatccttt tgggggcctc
      901 ccgctacgac gctcgctcca gctggggctt ccttactata cacagcgcgc atatttcacg
      961 gttgccagat gtcaagatga tgtttcacta taagacaggg tcgtcatggt tcctgctgct
     1021 gtccgcatcc attaccacaa ccctcacgac gacaacgatc acaacaaccc acgcctttgc
     1081 cccgcacacg cgcttatccg tgccccacgg tgcttcccga ctcgtcatga aggatttccc
     1141 caaacccaat ttggaagata cggacaatta ccgtttgtat cgtgatttgt cgcactcctt
     1201 ctccacgaca ctcaaggcac cgtcgccaga atcacgcaag aaggtagcca tcattggcgg
     1261 tggcttgtcc ggtctcgcct gtgccaagta cctcgccgac gccggacacc aacccgtcgt
     1321 ctacgaagca cgggacgtcc tcggcggcaa ggtctccgct tggcaagatg ccgacggaga
     1381 ctggatcgaa accggactac acattttctt tggtgcctac ccgaatatga tgaatctctt
     1441 tgccgaactt gatattcacg atcgtctcca gtggaaggtg cacaaaatga tctttgccat
     1501 gcaggaacta cccggagaat tcacaacctt tgatttcatt cccggcatac ccgcaccctt
     1561 caatttcgga ctcgctattc tcatgaacca gaaaatgctc actttgccgg agaaaataca
     1621 gacggctccc ccgctactgc ccatgctcgt acgcggacaa gactttatcg acgaacagga
     1681 cgaactctcc gtcctcgact ttatgcgcaa gtacgggatg cccgaacgca tcaacgaaga
     1741 agtctttatc agcatggcca aggcactcga tttcattgat cccgacaaat tgagtatgac
     1801 ggtcgttttg accgccatga atcgcttttt gaacgaagac aacgccctcc aaatggcctt
     1861 tctcgacggc aaccagcccg accggctctg tgcacccatg gtcgaacaca ttcaagcccg
     1921 tggcggtcaa gtcaacctca attcccccgt gcaagaaatc gtcacccgcg aagacggtag
     1981 tgtcgattac ctcctgatgc ggtccggaga aaaggtcgtt gccgacgaat acgtgtccgc
     2041 catgcccgtg gatattgtca aacgcatgtt gcccgaaaag tggcaaacaa tgccctactt
     2101 tcgtcaattc gacgaactcg aaggcattcc cgtcattaac ctccacatgt ggttcgatcg
     2161 caaactcaaa gccgtcgatc acttgtgctt ttcccgctcg ccgctgctgt ccgtttacgc
     2221 cgacatgagc gtcacctgca aggaatacta cgacgaatcc gcttccatgc tcgagctcgt
     2281 ctttgccccg tgcagtccac tcgccggtgg caacgtgaat tggattgcca agacggacga
     2341 agaaatcatc gacgcgacca tgggcgaact ggcccgactc tttcccaccg aaatcgccgc
     2401 tgatcccacc tggccggcca ccaagaacca gggccccaac ggtgaggcca aacttcgcaa
     2461 atacgccgtg gtcaaggtgc cccgctcggt ctacgccgcc attcccggcc gcaacaagta
     2521 ccgcccgagt caaaccactc caattgacaa tttcacgctg gccggggact ggacgtcaca
     2581 aaagtttctg ggatccatgg aaggcgccgt cctcgggggc aagctcgccg ccgaagtatt
     2641 ggcccgcaag gcggccaacc tacccgcacc cgaactcgcc aacaaacccg tgcgggatga
     2701 aattgtgcaa aaggcgcaaa cacacgaagc gcgtccaccg gccggagtca agggacaagg
     2761 tgcgattgcc tttggcggtg gcgccgtgct cgggacggaa aacaaagcct tgcttcggga
     2821 cgttgatcct agtcaattcg tggaagccta gaccttcctt aaaaatttaa ttttcattag
     2881 ttgcagtcac tccgctttgg tttcacagtc aggaataaca ctagctcgtc ttcaccatgg
     2941 atgccaatct cgcctattca tggtgtataa aagttcaaca tccaaagcta gaacttttgg
     3001 aaagagaaag aatatccgaa tagggcacgg cgtgccgtat tgttggagtg gactagcaga
     3061 aagtgaggaa ggcacaggat gagttttctc gagactagtt ctagagcggc cgccaccgcg
     3121 gtggagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg
     3181 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     3241 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     3301 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     3361 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     3421 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     3481 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     3541 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     3601 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     3661 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     3721 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     3781 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     3841 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     3901 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     3961 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     4021 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     4081 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     4141 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     4201 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     4261 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     4321 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     4381 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     4441 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     4501 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     4561 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     4621 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     4681 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     4741 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     4801 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     4861 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     4921 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     4981 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     5041 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     5101 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     5161 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     5221 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     5281 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
//
