LOCUS       40924_7121        4835 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pBS-35S-Ala-YFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   von Arnim A.
  TITLE     Vectors for Bioluminescence Resonance Energy Transfer
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4835)
  AUTHORS   von Arnim A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-DEC-2002) Botany, University of Tennessee, 1406 Circle
            Drive, Knoxville, TN 37996, USA
REFERENCE   3  (bases 1 to 4835)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4835)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-DEC-2002) Botany, University of Tennessee, 1406 Circle Drive, 
            Knoxville, TN 37996, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4835
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(6..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     669..685
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        789..1460
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     5'UTR           1460..1602
                     /note="translational enhancer; 5'UTR from tobacco etch
                     virus"
     regulatory      1601..1610
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1645..2361
                     /label=EYFP
                     /note="enhanced YFP"
     regulatory      2374..2584
                     /label=cauliflower mosaic virus 35S terminator
                     /note="cauliflower mosaic virus 35S terminator"
                     /regulatory_class="terminator"
     promoter        complement(2650..2668)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2689..2705)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2713..2729)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2737..2767)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2782..2803)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3091..3679)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3853..4710)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4711..4815)
                     /label=AmpR promoter
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gcccccctcg aggtcgacgg tatcgataag cttgatatcg aattcctgca ggtcaacatg
      721 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
      781 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
      841 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
      901 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
      961 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1021 aagcaagtgg attgatgtga taacatggtg gagcacgaca cacttgtcta ctccaaaaat
     1081 atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata
     1141 tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg
     1201 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa
     1261 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
     1321 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac
     1381 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt
     1441 tcatttcatt tggagaggac ctcgagaatt ctcaacacaa catatacaaa acaaacgaat
     1501 ctcaagcaat caagcattct acttctattg cagcaattta aatcatttct tttaaagcaa
     1561 aagcaatttt ctgaaaattt tcaccattta cgaacgatag ccatggcggc ggccgcaccg
     1621 gttgctgctg ctgctgctgc tctcatggtg agcaagggcg aggagctgtt caccggggtg
     1681 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     1741 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     1801 aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct gcagtgcttc
     1861 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     1921 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     1981 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     2041 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     2101 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     2161 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     2221 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc
     2281 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     2341 ggcatggacg agctgtacaa gagatctatc tagagtccgc aaaaatcacc agtctctctc
     2401 tacaaatcta tctctctcta tttttctcca gaataatgtg tgagtagttc ccagataagg
     2461 gaattagggt tcttataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg
     2521 tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca
     2581 gtgacctgca gcccggggga tccactagag cggccggccg ccaccgcggt ggagctccag
     2641 cttttgttcc ctttagtgag ggttaatttc gagcttggcg taatcatggt catagctgtt
     2701 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     2761 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     2821 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     2881 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     2941 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     3001 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     3061 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3121 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     3181 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     3241 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     3301 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     3361 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     3421 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     3481 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     3541 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     3601 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     3661 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     3721 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     3781 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     3841 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     3901 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     3961 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     4021 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     4081 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     4141 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     4201 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     4261 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     4321 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     4381 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     4441 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     4501 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     4561 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     4621 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     4681 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     4741 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     4801 aataggggtt ccgcgcacat ttccccgaaa agtgc
//
