LOCUS       40924_7066        3878 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBRcatTEM1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Tack DS, Ellefson JW, Thyer R, Wang B, Gollihar J, Forster MT, 
            Ellington AD.
  TITLE     Addicting diverse bacteria to a non-canonical amino acid
  JOURNAL   Nat. Chem. Biol. (2016) In press
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Tack DS, Ellefson JW, Thyer R, Wang B, Gollihar J, Forster MT, 
            Ellington AD.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2015) Molecular Biosciences, University of Texas, 
            2500 Speedway, Austin, TX 78660, USA
REFERENCE   3  (bases 1 to 3878)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3878)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. Chem. 
            Biol. (2016) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-NOV-2015) Molecular Biosciences, University of Texas, 2500 
            Speedway, Austin, TX 78660, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        48..150
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             151..807
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1449..1637
                     /codon_start=1
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    1742..1882
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2068..2656)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2830..3687)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3696..3796)
                     /label=AmpR promoter
ORIGIN
        1 gttgataccg ggaagccctg ggccaacttt tggcgaaaat gagacgttga tcggcacgta
       61 agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta
      121 tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc
      181 gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa
      241 tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa
      301 aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat
      361 ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct
      421 tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac
      481 gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac
      541 ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg
      601 gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt
      661 ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag
      721 gttcatcatg ccgtctgtga tggcttccat gtcggcagaa tgcttaatga attacaacag
      781 tactgcgatg agtggcaggg cggggcgtaa tttttttaag gcagttattg gtgcccttaa
      841 acgcctggtg ctacgcctga ataagtgata ataagcggat gaatggcaga aattcgaaag
      901 caaattcgac ccggtcgtcg gttcagggca gggtcgttaa atagccgctt atgtctattg
      961 ctggtttacc ggtttattga ctaccggaag cagtgtgacc gtgtgcttct ttgaggaccc
     1021 ggctaggctg gcggggttgc cttactggtt agcagaatga atcaccgata cgcgagcgaa
     1081 cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc aacaacatga atggtcttcg
     1141 gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc gccctgcacc attatgttcc
     1201 ggatctgcat cgcaggatgc tgctggctac cctgtggaac acctacatct gtattaacga
     1261 agcgctggca ttgaccctga gtgatttttc tctggtcccg ccgcatccat accgccagtt
     1321 gtttaccctc acaacgttcc agtaaccggg catgttcatc atcagtaacc cgtatcgtga
     1381 gcatcctctc tcgtttcatc ggtatcatta cccccatgaa cagaaatccc ccttacacgg
     1441 aggcatcagt gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc
     1501 agacattaac gcttctggag aaactcaacg agctggacgc ggatgaacag gcagacatct
     1561 gtgaatcgct tcacgaccac gctgatgagc tttaccgcag ctgcctcgcg cgtttcggtg
     1621 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     1681 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     1741 gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc
     1801 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     1861 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     1921 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     1981 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     2041 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     2101 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     2161 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     2221 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     2281 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     2341 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     2401 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     2461 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     2521 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     2581 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     2641 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     2701 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     2761 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     2821 ctcgagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     2881 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     2941 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     3001 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     3061 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     3121 gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     3181 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     3241 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     3301 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     3361 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     3421 gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa
     3481 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     3541 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     3601 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     3661 ggcgacacgg aaatgttgaa tactcataag ctttattcct ttttcaatat tattgaagca
     3721 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     3781 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     3841 ttatcatgac attaacctat aaaaataggc gtatcacg
//
