LOCUS       V013935                 6970 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013935
VERSION     V013935
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6970)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6970
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..38
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             86..1273
                     /label="TcR"
                     /note="tetracycline efflux protein"
     CDS             complement(1426..2904)
                     /gene="tolC"
                     /label="Outer membrane protein TolC"
                     /note="Outer membrane protein TolC from Escherichia coli
                     (strain K12). Accession#: P02930"
     promoter        complement(2927..2955)
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     promoter        3117..3147
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    3155..3171
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             3218..4228
                     /gene="int"
                     /label="Integrase/recombinase"
                     /note="Integrase/recombinase from Escherichia coli.
                     Accession#: P62590"
     CDS             4524..4712
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    4817..4957
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5143..5731)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5905..6762)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6763..6867)
                     /label="AmpR promoter"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
       61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
      121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
      181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
      241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
      301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
      361 cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
      421 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
      481 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
      541 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
      601 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
      661 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
      721 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
      781 ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
      841 tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
      901 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
      961 cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
     1021 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
     1081 tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
     1141 gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
     1201 tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
     1261 ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
     1321 attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
     1381 atatccatcg cgtccgccat ctccagcagc cgcacgcggc gctcagttac ggaaagggtt
     1441 atgaccgtta ctggtggtag tgcgtgcgga tgtttgctga acgactggtg ccgggctatc
     1501 aggcgcataa ccatcagcaa tagcattctg ttccggcgtt tgcggtgcaa cgttttccgg
     1561 attagtggaa accggtttgc tcagcgcatt gttcagtgcc agcagatcct gctcgttcaa
     1621 cgtacccaga gctgacttaa tattcagctg attaatcagg tagttataac gcgcattcgc
     1681 cagctcttgc ttggcgttgt acaacgtggt ggtcgcatcc aacacatcaa caatggtacg
     1741 cgtaccgacc gagtagcccg cttccatcgc gtctaatgag ctttgagcgg aaactacggc
     1801 ttgtttgtag gcgttaatgc tactgataga tgcattaatg ttgttgaagg aggaacgcac
     1861 ggtctgcacg acgctacgat gggcactttc cagttgctcg ctggcaccga caaagttgta
     1921 ctgtgcctgt ttcacctgcg agttaaccat tccgccctga taaatcggca gcgagaagct
     1981 caggccaact ttgttctggc ccatattgct atcgtcatac tgggtaccag cggcaccacg
     2041 ggttttcgaa ccgctataag aggtgtcaga aatcccggta gaagccgtta aatccagagt
     2101 cggtaagtga ccatcctgcg cctggcgaat ttgctcgcgc gccaggtcct ggctcaagcg
     2161 tgcctgtaac agcgacaggt tgcgtttttc ggcttctttc agcagcgcgt taaccggctg
     2221 tggtttgtcg gttttaaagt tttcgacatt cagcgcagcc agttccggat agtagttacc
     2281 ggtgatctgg cgcagctgct ctaccgcgtt atcaaggtta ttacgtgcgg tcacttcgtt
     2341 cgccagcacg gtatcgtact gtgcgcgggc gttctgcacg tcggtgatcg ctaccaggcc
     2401 cacgttaaaa cgttgggtgg tttgatctaa ttgacggtag atcgcttctt tttgtgcctg
     2461 tgtataggaa agaacgtcaa tagcattcaa cacgttgaaa taagcggtcg cggtgttgag
     2521 gatcaaggtt tgctgatcgg tctgatacgt gacgtcctga atccctgctg ctttttcctg
     2581 cagcgttaac gcacgccatt tcgacatatc aaaaatggat tgagttaact gcaaggacgc
     2641 actggtcgcg ttagagttga tgccgttcgc gtcgcggtag ccgttgctat aggtgtaatc
     2701 tgcacctaaa cctagctgtg gcagtaatgg actgcgcgct tcattaattt tttcaaaggc
     2761 agcatcacga tcggcggcag acttacgcaa ttccgggtta ctaaggcgtg cttgctgata
     2821 aacttgcatc aggttctcgg cctggctcaa cgaactgaac ccagaaaggc tcaggccgat
     2881 aagaatgggg agcaatttct tcatatctcg ggcagcgttg ggtcctcatt atacgagccg
     2941 atgattaatt gtcaaggcca cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc
     3001 taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc gagcgaacgt
     3061 gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg gtcttcttta
     3121 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     3181 tactagagaa agaggagaaa ggtttccgtg tttcgtaatg aaaaccgcca ctgcgccgtt
     3241 accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca
     3301 ttacagttta cgaaccgaac aggcttatgt ccactgggtt cgtgccttca tccgtttcca
     3361 cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc gaggcatttc tgtcctggct
     3421 ggcgaacgag cgcaaggttt cggtctccac gcatcgtcag gcattggcgg ccttgctgtt
     3481 cttctacggc aaggtgctgt gcacggatct gccctggctt caggagatcg gaagacctcg
     3541 gccgtcgcgg cgcttgccgg tggtgctgac cccggatgaa gtggttcgca tcctcggttt
     3601 tctggaaggc gagcatcgtt tgttcgccca gcttctgtat ggaacgggca tgcggatcag
     3661 tgagggtttg caactgcggg tcaaggatct ggatttcgat cacggcacga tcatcgtgcg
     3721 ggagggcaag ggctccaagg atcgggcctt gatgttaccc gagagcttgg cacccagcct
     3781 gcgcgagcag ctgtcgcgtg cacgggcatg gtggctgaag gaccaggccg agggccgcag
     3841 cggcgttgcg cttcccgacg cccttgagcg gaagtatccg cgcgccgggc attcctggcc
     3901 gtggttctgg gtttttgcgc agcacacgca ttcgaccgat ccacggagcg gtgtcgtgcg
     3961 tcgccatcac atgtatgacc agacctttca gcgcgccttc aaacgtgccg tagaacaagc
     4021 aggcatcacg aagcccgcca caccgcacac cctccgccac tcgttcgcga cggccttgct
     4081 ccgcagcggt tacgacattc gaaccgtgca ggatctgctc ggccattccg acgtctctac
     4141 gacgatgatt tacacgcatg tgctgaaagt tggcggtgcc ggagtgcgct caccgcttga
     4201 tgcgctgccg cccctcacta gtgagaggta gaagtctgga aacgcggaag tcagcgccct
     4261 gcaccattat gttccggatc tgcatcgcag gatgctgctg gctaccctgt ggaacaccta
     4321 catctgtatt aacgaagcgc tggcattgac cctgagtgat ttttctctgg tcccgccgca
     4381 tccataccgc cagttgttta ccctcacaac gttccagtaa ccgggcatgt tcatcatcag
     4441 taacccgtat cgtgagcatc ctctctcgtt tcatcggtat cattaccccc atgaacagaa
     4501 atccccctta cacggaggca tcagtgacca aacaggaaaa aaccgccctt aacatggccc
     4561 gctttatcag aagccagaca ttaacgcttc tggagaaact caacgagctg gacgcggatg
     4621 aacaggcaga catctgtgaa tcgcttcacg accacgctga tgagctttac cgcagctgcc
     4681 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     4741 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     4801 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg
     4861 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     4921 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac
     4981 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     5041 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     5101 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     5161 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     5221 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     5281 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     5341 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     5401 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     5461 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     5521 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     5581 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     5641 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     5701 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     5761 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     5821 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     5881 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     5941 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     6001 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     6061 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     6121 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     6181 gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc
     6241 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     6301 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     6361 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     6421 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     6481 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca
     6541 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     6601 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     6661 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     6721 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     6781 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     6841 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     6901 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     6961 tcttcaagaa
//
