LOCUS       40924_7011        7571 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBPS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7571)
  AUTHORS   Sharon-Gojman R, Maimon E, Leu S, Zarka A, Boussiba S.
  TITLE     Advanced Methods for Genetic Engineering of Haematococcus pluvialis 
            (Chlorophyceae, Volvocales)
  JOURNAL   Algal Res 10, 8-15 (2015)
REFERENCE   2  (bases 1 to 7571)
  AUTHORS   Sharon-Gojman R, Leu S, Zarka A, Maimon E, Boussiba S.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-FEB-2015) Microalgal Biotechnology Lab, Jacob 
            Blaustein Institutes for Desert Research, Sede Boquer Campus, 
            Ben-Gurion University, Sede-Boquer 8499000, Israel
REFERENCE   3  (bases 1 to 7571)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7571)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Algal Res 
            10, 8-15 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-FEB-2015) Microalgal Biotechnology Lab, Jacob Blaustein 
            Institutes for Desert Research, Sede Boquer Campus, Ben-Gurion 
            University, Sede-Boquer 8499000, Israel"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7571
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..28
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     54..70
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            79..4694
                     /gene="pdsMod4.1"
                     /label=pdsMod4.1
     regulatory      79..1049
                     /gene="pdsMod4.1"
                     /regulatory_class="promoter"
     mRNA            join(1010..1379,1469..1581,1687..2053,2406..2540,2834..2912,
                     3263..3612,3844..3914,4092..4694)
                     /gene="pdsMod4.1"
                     /product="norflurazon resistant phytoene desaturase"
                     /label=pdsMod4.1 mRNA
     5'UTR           1010..1049
                     /gene="pdsMod4.1"
     CDS             join(1050..1379,1469..1581,1687..2053,2406..2540,2834..2912,
                     3263..3612,3844..3914,4092..4356)
                     /codon_start=1
                     /gene="pdsMod4.1"
                     /product="norflurazon resistant phytoene desaturase"
                     /label=pdsMod4.1
                     /protein_id="AKO71909.1"
                     /translation="MQTAMRGQASGSGCTSGRQARGHWSRRSVRERGALRVVAKDYPTP
                     DFQSSDTYQEALSLSTKLRNAPRPAKPLRVVIAGAGLAGLSAAKYLADAGHHPVVLEGR
                     DVLGGKVAAWKDEDGDWYETGLHIFFGAYPNIQNLFKELGIQDRLQWKEHSMIFAMPDA
                     PGEFSRFDFPELPAPWNGIIAILRNNQMLSWPEKIRFAIGLLPAIIFGQRYCEEQDELT
                     VTEWMRKQGVPDRVNEEVFIAMAKALNFINPDDLSMTVVLTALNRFLQEQHGSKMAFLD
                     GAPPERLCQPMVDYFKARGGDLMFNSRVKQIVLNDDKSVKHLALTNGQTVEGDLYISAM
                     PVDIMKILMPDPWASMPYFKQLNGLEGVPVINIHIWFDRKLTTVDHLLFSRSPLLSVYA
                     DMSTTCKEYADDKKSMLELVFAPAKEWIGRPDEEIIAATMTELERLFPTEVRADQSMAK
                     ILKYKVVKTPLSVYKSTAGREKFRPTQRSPISNFYLAGDYTKQKYRASMEGAVFSGKLV
                     TEAIVEDWSARGVTSSAASRQPALAAAGVVAGSAAVIGAALVAASGAIAGGM"
     misc_feature    4157..4158
                     /gene="pdsMod4.1"
                     /label=L504R mutation
                     /note="L504R mutation"
     3'UTR           4357..4694
                     /gene="pdsMod4.1"
     primer_bind     complement(4714..4730)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(4767..4785)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4806..4822)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4830..4846)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4854..4884)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4899..4920)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5208..5796)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5970..6827)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6828..6932)
                     /label=AmpR promoter
     rep_origin      6959..7414
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     7555..7571
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gagcgcgcgt aatacgactc actatagggc gaattgggta ccgggccccc cctcgaggtc
       61 gacggtatcg ataagcttct gtacgccatt gtacgccgtt gactgatgga ctgattgttt
      121 gactgatgga agaactgtgt agacctcagc cccctcagca cattagcata tctcgacacc
      181 tcagccatgc gcacaccgag ttcgccttgt catgcgcagc caccctgtag aatgaagtca
      241 cgtaagtagt ggctgccgcg aggtcgcctt ggtattgtct ctggcttgat atagcgctga
      301 ctgacgggtg gtgacggtgc tcgtgcgcta tagatgccac tagaggcccg cccacctcgc
      361 gcctgagccg caaaacccgg cggggtcgtc acgatgaggg catcttacct cgacgatgta
      421 ccgaccccgg gagctgtccc gctggactgg cggatcgcgg ctgcccaact tgggcactga
      481 gcgttggctg tggatcaaca tcattggcgc cgatgtcagc ctgggccagc aggtgcagcg
      541 gctgaccggc tatggaccgg ctatggaccg gctacgatag cggatgcagc gttcctccgc
      601 atgcagagat gcactggacc agtaccggag acggcgccag cgggatacac ggattagtgc
      661 gcctgaactg cggtgtgccc catgaatgac catgccagcc ccaaggggcg gcattgaagc
      721 ggccctagag agctagtggc aaggcagcac tagacaactt atgagctaag gtcagggagc
      781 tatgaggctg caagattagg ggcctgattt gagttgttca gtcgcacgtc ttcacgacgt
      841 tacggctgcc atcttaccca gtcggctaaa gcaggccacc accattggcg tcacgcgtga
      901 tcgtcttgaa atgacagcta gtccaaacac caggctcagc tcgtatggtt tggcgagggc
      961 gtttcgtcgg ttgaacgacg tgcctttaat gaaaattcgt ggccatacca ttcatcgctt
     1021 gagccaacca tccagcatca actctaacaa tgcagacagc aatgcgtggc caagccagcg
     1081 gttcaggatg cacttccggc aggcaagctc gcggtcattg gtcaaggcgc tctgtgcgtg
     1141 agcgaggtgc tctcagggtg gttgccaagg actaccccac accggatttc cagtcatcgg
     1201 acacatacca ggaggccctg tccctgtcga ccaagctgcg aaatgcaccc cggcccgcca
     1261 agcctttgcg cgttgtcatt gctggggccg gcctggcagg cctctcggct gcaaaatacc
     1321 tggcagatgc agggcaccac ccagttgtgc tggagggccg cgacgtgcta ggcggcaagg
     1381 taaggacttg cgagtgtcga atgccacgcc ggcccgttcc aaacgcccac aagctgtccg
     1441 gccacctcac aaactcattg gcgcacaggt ggccgcgtgg aaggacgagg acggtgattg
     1501 gtacgagact ggccttcaca ttttctttgg tgcttacccc aacatccaga acctgttcaa
     1561 agagctgggc atccaagaca ggtaggaggt gcacccagta gtgtcaacgc attgcctagc
     1621 aagcgcctca gtcaacaaga ccaccatgag cactgttctt gatgtgggca tgtgcctgcc
     1681 ttgcaggctg cagtggaagg agcactccat gatctttgcc atgccagacg cccctgggga
     1741 gttttctcgc tttgacttcc ctgagctgcc tgcgccctgg aacggcatca tcgccatctt
     1801 gcggaacaac cagatgctca gctggcctga gaagatccgg tttgccatcg gcctgctgcc
     1861 tgctatcatc tttgggcagc gctactgtga agagcaggat gagctgaccg tgacagagtg
     1921 gatgcggaag cagggcgtgc cagaccgagt caatgaggaa gtgttcatcg ccatggccaa
     1981 ggccttgaac ttcatcaacc ctgacgacct atccatgaca gtagtgctca ccgcactgaa
     2041 ccgtttcctg caggcaagtg ttgggccctg gcagtcaggg gtcaggggcc tgcagggcca
     2101 gcttgcatct gcccgacctt cagggccgcc gcctccctca caaagcatgc accctccaca
     2161 acccgcctgt ctgtctgccc aacaccagct tatggtccaa atccaccaca accgcctctc
     2221 accctcctgc ccctgctttc agcctgagca ctgcagtcct gtcaccagca aatgtgtggc
     2281 ggctgcactt cttgtccctg ccgccaatcg ctttcagtgt ggcctggcca gcttgtgaca
     2341 gtggctagag ctccaagctc tgcaaagact gagacaagct gtggtttgca ttgcctgggc
     2401 tgcaggagca gcatggcagc aagatggcat tcttggacgg cgccccacct gagcgcctct
     2461 gtcagccaat ggtggactac ttcaaggcac gaggcggcga cctcatgttc aactcccggg
     2521 tcaagcagat tgtgttgaat gtgaggtccc tggctctcag cctcgccgcc tcctgcagac
     2581 gctgcatgcc atgtgcagta tcaccatgcc acacacgtcg gcatcagctg caggcctagc
     2641 tctcgcgccg cagtcacagt tgaacccaac acatgtgcaa gtcatcaccc tgtgcagcag
     2701 gcaggggcag gctgggtgcc tgggcctgtc atccactgca gccacagtct tgagtggagg
     2761 gtacagtcat taactctgcc catgaggtgt cagagtcttt catgggtgct atatggtcgc
     2821 tcttggcgtg caggacgaca agagtgtcaa gcacctggcc ctcaccaatg gtcagacagt
     2881 tgagggcgac ctctacatct cagccatgcc aggtgtgtgc ctatggtgca gctgggctgt
     2941 gtgcccctaa gcagccacac aagcactgga tgccatcacc cattgctgca tgacataggc
     3001 acaaggatga tagccaacga catggatgag gcacctgggc acagaggcac acgttgtggc
     3061 ctgcacagcc tgagtcatga attgaggggg gagagccagg taggctgtaa ttgtggcact
     3121 gacctccaca aaaccataac acaccagatt gactgagtcc agttaggctt gggggcttcc
     3181 cttcagtttg ttcagaacca ggatgcttgg ctcctcccta ccatgcgctg acatgtgtgc
     3241 ttgcattgat gtggtctcgc agtggacatc atgaagatcc tcatgcctga cccctgggcc
     3301 tccatgccct acttcaagca gctgaacggc ctggaggggg tgcctgtgat caacatccac
     3361 atctggttcg atcgcaagct gactacggtg gaccacctgt tgttcagtcg gtcaccgctg
     3421 ctgtctgtgt acgctgacat gagcaccacg tgcaaggagt atgctgacga caagaagagc
     3481 atgcttgagc tggtgtttgc gccggccaag gagtggattg gccggcctga cgaggagatc
     3541 atcgcagcca ccatgacaga gctggagcgg ctgttcccga cagaggtcag ggctgaccag
     3601 tccatggcca aggtaggcgg gtcaggcacc agccagcact gccaggcctg caggttaaga
     3661 aggcttgtga ggggtgcagc tctgggcaga tagcagctac agccggctaa cggtgccggg
     3721 gttgtgagtg aatggcgccc agtgcctgaa gcacccccct gctcagcttg ctctgctgtg
     3781 ccagcattgg ctgcctgctg tcgaagtact tgtggtgatg ggtgatggtg gcatgatggg
     3841 cagatcttga agtacaaggt ggtgaagact ccattgtctg tgtacaagtc cactgctggg
     3901 cgggagaagt tcaggtgagc ctcgcgacag ccgacagtgg aatgcactgc agcccttgtg
     3961 gctgcctgac tggctttctt gtcagccgct ctctcgtgac cataactgat gctgccgtac
     4021 cttatctgct gctgctgctg tcatgctggt gctaatggtg gtgccccggt gctgctgctg
     4081 ctgtgctgca gacccactca gcgctcccca atctccaact tctaccttgc gggtgattac
     4141 accaagcaga agtaccgcgc ctccatggag ggtgcagtgt tctccggcaa gctggtcacc
     4201 gaagccattg tagaggactg gagcgctcgg ggtgtgacca gcagcgctgc cagccgccag
     4261 cctgccctgg ctgcagccgg tgtggtggca gggtcggctg cagtgattgg ggcggcgttg
     4321 gttgcagcca gtggtgcaat agccggtggg atgtaagtga tgaaagaatg gcacctggta
     4381 gatgaggcag catttataga aatggcagca ccctcgctgc ctaatgcttg tggtgtatac
     4441 tatttgacct cgggagttga ggcccgcagc cattgtgtgg ctatcgcatg gggtggtgca
     4501 tcgctgctca atgccgacaa cattgggatg gcctagaact tattgctagg tagtgtgacc
     4561 tggacgagag attgggtatg ggtctggtga ggaagggcag gcactatggg cgagcattct
     4621 gagaggttgg gcaggcgtgc tgtagtcctg cgatgagttg gagtgtctaa acggcagtaa
     4681 cggatatggg gtcagaattc ctgcagcccg ggggatccac tagttctaga gcggccgcca
     4741 ccgcggtgga gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
     4801 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     4861 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     4921 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     4981 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     5041 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     5101 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     5161 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     5221 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     5281 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     5341 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     5401 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     5461 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     5521 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     5581 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     5641 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     5701 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     5761 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     5821 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     5881 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     5941 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     6001 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     6061 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     6121 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     6181 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     6241 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     6301 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     6361 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     6421 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     6481 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     6541 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     6601 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     6661 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     6721 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     6781 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     6841 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     6901 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     6961 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     7021 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     7081 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     7141 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     7201 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     7261 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     7321 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     7381 gcgaatttta acaaaatatt aacgcttaca atttccattc gccattcagg ctgcgcaact
     7441 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     7501 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     7561 cgacggccag t
//
