LOCUS       40924_7006        8599 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBPL, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8599)
  AUTHORS   Sharon-Gojman R, Maimon E, Leu S, Zarka A, Boussiba S.
  TITLE     Advanced Methods for Genetic Engineering of Haematococcus pluvialis 
            (Chlorophyceae, Volvocales)
  JOURNAL   Algal Res 10, 8-15 (2015)
REFERENCE   2  (bases 1 to 8599)
  AUTHORS   Sharon-Gojman R, Leu S, Zarka A, Maimon E, Boussiba S.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-FEB-2015) Microalgal Biotechnology Lab, Jacob 
            Blaustein Institutes for Desert Research, Sede Boquer Campus, 
            Ben-Gurion University, Sede-Boquer 8499000, Israel
REFERENCE   3  (bases 1 to 8599)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8599)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Algal Res 
            10, 8-15 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-FEB-2015) Microalgal Biotechnology Lab, Jacob Blaustein 
            Institutes for Desert Research, Sede Boquer Campus, Ben-Gurion 
            University, Sede-Boquer 8499000, Israel"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8599
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     31..47
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        57..75
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     101..117
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            126..5769
                     /gene="pdsMod4.1"
                     /label=pdsMod4.1
     regulatory      126..2124
                     /gene="pdsMod4.1"
                     /regulatory_class="promoter"
     mRNA            join(2085..2454,2544..2656,2762..3128,3481..3615,3909..3987,
                     4338..4687,4919..4989,5167..5769)
                     /gene="pdsMod4.1"
                     /product="norflurazon resistant phytoene desaturase"
                     /label=pdsMod4.1 mRNA
     5'UTR           2085..2124
                     /gene="pdsMod4.1"
     CDS             join(2125..2454,2544..2656,2762..3128,3481..3615,3909..3987,
                     4338..4687,4919..4989,5167..5431)
                     /codon_start=1
                     /gene="pdsMod4.1"
                     /product="norflurazon resistant phytoene desaturase"
                     /label=pdsMod4.1
                     /protein_id="AKO71907.1"
                     /translation="MQTAMRGQASGSGCTSGRQARGHWSRRSVRERGALRVVAKDYPTP
                     DFQSSDTYQEALSLSTKLRNAPRPAKPLRVVIAGAGLAGLSAAKYLADAGHHPVVLEGR
                     DVLGGKVAAWKDEDGDWYETGLHIFFGAYPNIQNLFKELGIQDRLQWKEHSMIFAMPDA
                     PGEFSRFDFPELPAPWNGIIAILRNNQMLSWPEKIRFAIGLLPAIIFGQRYCEEQDELT
                     VTEWMRKQGVPDRVNEEVFIAMAKALNFINPDDLSMTVVLTALNRFLQEQHGSKMAFLD
                     GAPPERLCQPMVDYFKARGGDLMFNSRVKQIVLNDDKSVKHLALTNGQTVEGDLYISAM
                     PVDIMKILMPDPWASMPYFKQLNGLEGVPVINIHIWFDRKLTTVDHLLFSRSPLLSVYA
                     DMSTTCKEYADDKKSMLELVFAPAKEWIGRPDEEIIAATMTELERLFPTEVRADQSMAK
                     ILKYKVVKTPLSVYKSTAGREKFRPTQRSPISNFYLAGDYTKQKYRASMEGAVFSGKLV
                     TEAIVEDWSARGVTSSAASRQPALAAAGVVAGSAAVIGAALVAASGAIAGGM"
     misc_feature    5232..5233
                     /gene="pdsMod4.1"
                     /label=L504R mutation
                     /note="L504R mutation"
     3'UTR           5432..5769
                     /gene="pdsMod4.1"
     primer_bind     complement(5789..5805)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(5842..5860)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5881..5897)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5905..5921)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5929..5959)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5974..5995)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6283..6871)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7045..7902)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7903..8007)
                     /label=AmpR promoter
     rep_origin      8034..8489
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
       61 acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcgac ggtatcgata
      121 agcttgcaca gttagaggcg tggttgcaga cgtaggtgag cagttgggtg ctgctgctgc
      181 tgtgatctga tacatgttga aaactgagcg gaatggccat gtattgttgt ttgattactc
      241 atggccatgc actgagcatg cagcaagcca tacccatcca ggagttgtct tcacttcctt
      301 tcccgcgctt gcttgctttc atccgctttc tttccatatg ctgtgttgtg atccctccct
      361 cccctcctcc ctccttaccc tgccccaccc ctggtggtgt gtggccaggt ggctgtgacg
      421 cactgcctgc tgagcgctga cctggtgagc atgagcgcca ggctgggccg agctgcctgg
      481 gtggccacca ctcggctgta tgctgctcag caagaggtgg caggccaggc ccatgtggca
      541 gccatgcacc tggtggcaat cggggatagg gctgcggcag aagcagtgta caggtgggcg
      601 cgggcaggag cagggacagg ggctataggg tccccggcca ggtctaccct gactgctggg
      661 gttaaggcta gggctagggc tagggctagg gctagggcta gggctagggc tagggctagg
      721 gctagggcta gggctagggc tagggctagg gctagggcta gggctagggc tagggctagg
      781 gctagggcta gggacaaggg ttgggggcga agggtggggg tttgcagcag ccagggtcag
      841 ccctgggcca acagtagcta ctgcacatcc ccacaatctc ctacaggcag cacaggctaa
      901 cgtgcgcgga ctgcatcacc gggtacacag ttgatgacat tcttatcgaa gcatctcacc
      961 ctacgaccca tcacaattag ttgcaagggt tcctgtcggt gttgaggttt gagttggtct
     1021 gtatgcaggg gcctgatgga aaaactgtag tagctgagtg agattaccca cagccaagca
     1081 tgttgttcca tgttgaacag gttttgtttg gtctgatgga aaaactgttg aaatgcttcc
     1141 tgtcatcgtt ccgctgtacg ccattgtacg ccgttgactg atggactgat tgtttgactg
     1201 atggaagaac tgtgtagacc tcagccccct cagcacatta gcatatctcg acacctcagc
     1261 catgcgcaca ccgagttcgc cttgtcatgc gcagccaccc tgtagaatga agtcacgtaa
     1321 gtagtggctg ccgcgaggtc gccttggtat tgtctctggc ttgatatagc gctgactgac
     1381 gggtggtgac ggtgctcgtg cgctatagat gccactagag gcccgcccac ctcgcgcctg
     1441 agccgcaaaa cccggcgggg tcgtcacgat gagggcatct tacctcgacg atgtaccgac
     1501 cccgggagct gtcccgctgg actggcggat cgcggctgcc caacttgggc actgagcgtt
     1561 ggctgtggat caacatcatt ggcgccgatg tcagcctggg ccagcaggtg cagcggctga
     1621 ccggctatgg accggctatg gaccggctac gatagcggat gcagcgttcc tccgcatgca
     1681 gagatgcact ggaccagtac cggagacggc gccagcggga tacacggatt agtgcgcctg
     1741 aactgcggtg tgccccatga atgaccatgc cagccccaag gggcggcatt gaagcggccc
     1801 tagagagcta gtggcaaggc agcactagac aacttatgag ctaaggtcag ggagctatga
     1861 ggctgcaaga ttaggggcct gatttgagtt gttcagtcgc acgtcttcac gacgttacgg
     1921 ctgccatctt acccagtcgg ctaaagcagg ccaccaccat tggcgtcacg cgtgatcgtc
     1981 ttgaaatgac agctagtcca aacaccaggc tcagctcgta tggtttggcg agggcgtttc
     2041 gtcggttgaa cgacgtgcct ttaatgaaaa ttcgtggcca taccattcat cgcttgagcc
     2101 aaccatccag catcaactct aacaatgcag acagcaatgc gtggccaagc cagcggttca
     2161 ggatgcactt ccggcaggca agctcgcggt cattggtcaa ggcgctctgt gcgtgagcga
     2221 ggtgctctca gggtggttgc caaggactac cccacaccgg atttccagtc atcggacaca
     2281 taccaggagg ccctgtccct gtcgaccaag ctgcgaaatg caccccggcc cgccaagcct
     2341 ttgcgcgttg tcattgctgg ggccggcctg gcaggcctct cggctgcaaa atacctggca
     2401 gatgcagggc accacccagt tgtgctggag ggccgcgacg tgctaggcgg caaggtaagg
     2461 acttgcgagt gtcgaatgcc acgccggccc gttccaaacg cccacaagct gtccggccac
     2521 ctcacaaact cattggcgca caggtggccg cgtggaagga cgaggacggt gattggtacg
     2581 agactggcct tcacattttc tttggtgctt accccaacat ccagaacctg ttcaaagagc
     2641 tgggcatcca agacaggtag gaggtgcacc cagtagtgtc aacgcattgc ctagcaagcg
     2701 cctcagtcaa caagaccacc atgagcactg ttcttgatgt gggcatgtgc ctgccttgca
     2761 ggctgcagtg gaaggagcac tccatgatct ttgccatgcc agacgcccct ggggagtttt
     2821 ctcgctttga cttccctgag ctgcctgcgc cctggaacgg catcatcgcc atcttgcgga
     2881 acaaccagat gctcagctgg cctgagaaga tccggtttgc catcggcctg ctgcctgcta
     2941 tcatctttgg gcagcgctac tgtgaagagc aggatgagct gaccgtgaca gagtggatgc
     3001 ggaagcaggg cgtgccagac cgagtcaatg aggaagtgtt catcgccatg gccaaggcct
     3061 tgaacttcat caaccctgac gacctatcca tgacagtagt gctcaccgca ctgaaccgtt
     3121 tcctgcaggc aagtgttggg ccctggcagt caggggtcag gggcctgcag ggccagcttg
     3181 catctgcccg accttcaggg ccgccgcctc cctcacaaag catgcaccct ccacaacccg
     3241 cctgtctgtc tgcccaacac cagcttatgg tccaaatcca ccacaaccgc ctctcaccct
     3301 cctgcccctg ctttcagcct gagcactgca gtcctgtcac cagcaaatgt gtggcggctg
     3361 cacttcttgt ccctgccgcc aatcgctttc agtgtggcct ggccagcttg tgacagtggc
     3421 tagagctcca agctctgcaa agactgagac aagctgtggt ttgcattgcc tgggctgcag
     3481 gagcagcatg gcagcaagat ggcattcttg gacggcgccc cacctgagcg cctctgtcag
     3541 ccaatggtgg actacttcaa ggcacgaggc ggcgacctca tgttcaactc ccgggtcaag
     3601 cagattgtgt tgaatgtgag gtccctggct ctcagcctcg ccgcctcctg cagacgctgc
     3661 atgccatgtg cagtatcacc atgccacaca cgtcggcatc agctgcaggc ctagctctcg
     3721 cgccgcagtc acagttgaac ccaacacatg tgcaagtcat caccctgtgc agcaggcagg
     3781 ggcaggctgg gtgcctgggc ctgtcatcca ctgcagccac agtcttgagt ggagggtaca
     3841 gtcattaact ctgcccatga ggtgtcagag tctttcatgg gtgctatatg gtcgctcttg
     3901 gcgtgcagga cgacaagagt gtcaagcacc tggccctcac caatggtcag acagttgagg
     3961 gcgacctcta catctcagcc atgccaggtg tgtgcctatg gtgcagctgg gctgtgtgcc
     4021 cctaagcagc cacacaagca ctggatgcca tcacccattg ctgcatgaca taggcacaag
     4081 gatgatagcc aacgacatgg atgaggcacc tgggcacaga ggcacacgtt gtggcctgca
     4141 cagcctgagt catgaattga ggggggagag ccaggtaggc tgtaattgtg gcactgacct
     4201 ccacaaaacc ataacacacc agattgactg agtccagtta ggcttggggg cttcccttca
     4261 gtttgttcag aaccaggatg cttggctcct ccctaccatg cgctgacatg tgtgcttgca
     4321 ttgatgtggt ctcgcagtgg acatcatgaa gatcctcatg cctgacccct gggcctccat
     4381 gccctacttc aagcagctga acggcctgga gggggtgcct gtgatcaaca tccacatctg
     4441 gttcgatcgc aagctgacta cggtggacca cctgttgttc agtcggtcac cgctgctgtc
     4501 tgtgtacgct gacatgagca ccacgtgcaa ggagtatgct gacgacaaga agagcatgct
     4561 tgagctggtg tttgcgccgg ccaaggagtg gattggccgg cctgacgagg agatcatcgc
     4621 agccaccatg acagagctgg agcggctgtt cccgacagag gtcagggctg accagtccat
     4681 ggccaaggta ggcgggtcag gcaccagcca gcactgccag gcctgcaggt taagaaggct
     4741 tgtgaggggt gcagctctgg gcagatagca gctacagccg gctaacggtg ccggggttgt
     4801 gagtgaatgg cgcccagtgc ctgaagcacc cccctgctca gcttgctctg ctgtgccagc
     4861 attggctgcc tgctgtcgaa gtacttgtgg tgatgggtga tggtggcatg atgggcagat
     4921 cttgaagtac aaggtggtga agactccatt gtctgtgtac aagtccactg ctgggcggga
     4981 gaagttcagg tgagcctcgc gacagccgac agtggaatgc actgcagccc ttgtggctgc
     5041 ctgactggct ttcttgtcag ccgctctctc gtgaccataa ctgatgctgc cgtaccttat
     5101 ctgctgctgc tgctgtcatg ctggtgctaa tggtggtgcc ccggtgctgc tgctgctgtg
     5161 ctgcagaccc actcagcgct ccccaatctc caacttctac cttgcgggtg attacaccaa
     5221 gcagaagtac cgcgcctcca tggagggtgc agtgttctcc ggcaagctgg tcaccgaagc
     5281 cattgtagag gactggagcg ctcggggtgt gaccagcagc gctgccagcc gccagcctgc
     5341 cctggctgca gccggtgtgg tggcagggtc ggctgcagtg attggggcgg cgttggttgc
     5401 agccagtggt gcaatagccg gtgggatgta agtgatgaaa gaatggcacc tggtagatga
     5461 ggcagcattt atagaaatgg cagcaccctc gctgcctaat gcttgtggtg tatactattt
     5521 gacctcggga gttgaggccc gcagccattg tgtggctatc gcatggggtg gtgcatcgct
     5581 gctcaatgcc gacaacattg ggatggccta gaacttattg ctaggtagtg tgacctggac
     5641 gagagattgg gtatgggtct ggtgaggaag ggcaggcact atgggcgagc attctgagag
     5701 gttgggcagg cgtgctgtag tcctgcgatg agttggagtg tctaaacggc agtaacggat
     5761 atggggtcag aattcctgca gcccggggga tccactagtt ctagagcggc cgccaccgcg
     5821 gtggagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg
     5881 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     5941 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     6001 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     6061 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     6121 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     6181 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     6241 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     6301 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     6361 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     6421 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     6481 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     6541 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     6601 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     6661 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     6721 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     6781 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     6841 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     6901 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     6961 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     7021 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     7081 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     7141 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     7201 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     7261 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     7321 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     7381 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     7441 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     7501 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     7561 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     7621 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     7681 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     7741 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     7801 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     7861 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     7921 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     7981 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc
     8041 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     8101 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     8161 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     8221 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     8281 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     8341 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     8401 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     8461 ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg caactgttgg
     8521 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     8581 gcaaggcgat taagttggg
//
