LOCUS       40924_6996       11489 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBOMBER, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11489)
  AUTHORS   Plackett AR, Conway SJ, Hewett Hazelton KD, Rabbinowitsch EH, 
            Langdale JA, Di Stilio VS.
  TITLE     LEAFY maintains apical stem cell activity during shoot development 
            in the fern Ceratopteris richardii
  JOURNAL   Elife 7, e39625 (2018)
  PUBMED    30355440
REFERENCE   2  (bases 1 to 11489)
  AUTHORS   Plackett ARG., Rabbinowitsch EH, Langdale JA.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2018) Department of Plant Sciences, University of 
            Cambridge, Downing Street, Cambridge, Cambridgeshire CB2 3EA, United
            Kingdom
REFERENCE   3  (bases 1 to 11489)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11489)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife 7, 
            e39625 (2018)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-SEP-2018) Department of Plant Sciences, University of Cambridge,
            Downing Street, Cambridge, Cambridgeshire CB2 3EA, United Kingdom"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(477..501)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    757..778
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        793..823
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    831..847
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     855..871
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        889..907
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        1068..1247
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             1283..2305
                     /codon_start=1
                     /product="HPT"
                     /label=HPT
                     /protein_id="AYO90637.1"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWARTAPKSGTRARGFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     terminator      2503..2755
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    complement(3051..3075)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     oriT            3630..3739
                     /label=oriT
                     /note="incP origin of transfer"
     mobile_element  3799..4566
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             4566..4916
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="PTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAVGQGYKI
                     TGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEEKQDELG
                     KVMMGVVRPRAEP"
     CDS             5191..6336
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(7699..8287)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             9384..10172
                     /codon_start=1
                     /product="SpecR"
                     /label=SpecR
                     /protein_id="AYO90638.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      complement(10777..11489)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcgacatctt gctgcgttcg gatattttcg tggagttccc gccacagacc cggattgaag
       61 gcgagatcca gcaactcgcg ccagatcatc ctgtgacgga actttggcgc gtgatgactg
      121 gccaggacgt cggccgaaag agcgacaagc agatcacgat tttcgacagc gtcggatttg
      181 cgatcgagga tttttcggcg ctgcgctacg tccgcgaccg cgttgaggga tcaagccaca
      241 gcagcccact cgaccttcta gccgacccag acgagccaag ggatcttttt ggaatgctgc
      301 tccgtcgtca ggctttccga cgtttgggtg gttgaacaga agtcattatc gtacggaatg
      361 ccagcactcc cgaggggaac cctgtggttg gcatgcacat acaaatggac gaacggataa
      421 accttttcac gcccttttaa atatccgtta ttctaataaa cgctcttttc tcttaggttt
      481 acccgccaat atatcctgtc aaacactgat agtttaaact gaaggcggga aacgacaatc
      541 tgatcatgag cggagaatta agggagtcac gttatgaccc ccgccgatga cgcgggacaa
      601 gccgttttac gtttggaact gacagaaccg caacgattga aggagccact cagccccaat
      661 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      721 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      781 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      841 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat ttaggtgaca
      901 ctatagaata ctcaagctat gcatccaacg cgttgggagc tctcccatat cgacctgcag
      961 gcggccgcat atgggagagc tcgaattatc atacatgaga attaagggag tcacgttatg
     1021 accccgccga tgacgcggga caagccgttt tacgtttgga actgacagaa ccgcaacgtt
     1081 gaaggagcca ctgagccgcg ggtttctgga gtttaatgag ctaagcacat acgtcagaaa
     1141 ccattattgc gcgttcaaaa gtcgcctaag gtcactatca gctagcaaat atttcttgtc
     1201 aaaaatgctc cactgacgtt ccataaattc ccctcggtat ccaattagag tctcatattc
     1261 actctcaact cgaatccccc ctatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa
     1321 gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga
     1381 atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg
     1441 cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc
     1501 gattccggaa gtgcttgaca ttggggagtt cagcgagagc ctgacctatt gcatctcccg
     1561 ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttcttca
     1621 gccggtcgcg gaggctatgg atgctatcgc tgcggccgat cttagccaga cgagcgggtt
     1681 cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc
     1741 gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc
     1801 cgtcgcgcag gctctcgatg agctgatgct ttgggcgagg actgccccga agtccggcac
     1861 tcgtgcacgc ggattcggct ccaacaatgt cctgacggac aatggccgca taacagcggt
     1921 cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt
     1981 ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc
     2041 ggagcttgca ggatcgccaa ggctccgggc gtatatgctc cgcattggtc ttgaccaact
     2101 ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga
     2161 cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc
     2221 ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag
     2281 cactcgtccg agggcaaagg aatagagtag atgccgaccg aacaagagct gatttcgaga
     2341 acgcctcagc cagcaactcg cgcgagccta gcaaggcaaa tgcgagagaa cggccttacg
     2401 cttggtggca cagttctcgt ccacagttcg ctaagctcgc tcggctgggt cgcgggaggg
     2461 ccggtcgcag tgattcagga attaattccc tagagtcaag cagatcgttc aaacatttgg
     2521 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
     2581 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
     2641 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
     2701 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgaccg
     2761 gcatgcaagc tgataattct cgagccggtc ttgcgatgat tatcatataa tttctgttga
     2821 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt
     2881 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg
     2941 caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcga attaattcag
     3001 tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca
     3061 caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca
     3121 ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg
     3181 cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt
     3241 ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg
     3301 ctgcctgtga tcaaatatca tctccctcgc agagatccga attatcagcc ttcttattca
     3361 tttctcgctt aaccgtgaca ggctgtcgat cttgagaact atgccgacat aataggaaat
     3421 cgctggataa agccgctgag gaagctgagt ggcgctattt ctttagaagt gaacgttgac
     3481 gatcgtcgac ggatcttttc cgctgcataa ccctgcttcg gggtcattat agcgattttt
     3541 tcggtatatc catccttttt cgcacgatat acaggatttt gccaaagggt tcgtgtagac
     3601 tttccttggt gtatccaacg gcgtcagccg ggcaggatag gtgaagtagg cccacccgcg
     3661 agcgggtgtt ccttcttcac tgtcccttat tcgcacctgg cggtgctcaa cgggaatcct
     3721 gctctgcgag gctggccggc taccgccggc gtaacagatg agggcaagcg gatggctgat
     3781 gaaaccaagc caaccagggg tgatgctgcc aacttactga tttagtgtat gatggtgttt
     3841 ttgaggtgct ccagtggctt ctgtttctat cagctgtccc tcctgttcag ctactgacgg
     3901 ggtggtgcgt aacggcaaaa gcaccgccgg acatcagcgc tatctctgct ctcactgccg
     3961 taaaacatgg caactgcagt tcacttacac cgcttctcaa cccggtacgc accagaaaat
     4021 cattgatatg gccatgaatg gcgttggatg ccgggcaaca gcccgcatta tgggcgttgg
     4081 cctcaacacg attttacgtc acttaaaaaa ctcaggccgc agtcggtaac ctcgcgcata
     4141 cagccgggca gtgacgtcat cgtctgcgcg gaaatggacg aacagtgggg ctatgtcggg
     4201 gctaaatcgc gccagcgctg gctgttttac gcgtatgaca gtctccggaa gacggttgtt
     4261 gcgcacgtat tcggtgaacg cactatggcg acgctggggc gtcttatgag cctgctgtca
     4321 ccctttgacg tggtgatatg gatgacggat ggctggccgc tgtatgaatc ccgcctgaag
     4381 ggaaagctgc acgtaatcag caagcgatat acgcagcgaa ttgagcggca taacctgaat
     4441 ctgaggcagc acctggcacg gctgggacgg aagtcgctgt cgttctcaaa atcggtggag
     4501 ctgcatgaca aagtcatcgg gcattatctg aacataaaac actatcaata agttggagtc
     4561 attacccaac caggaagggc agcccaccta tcaaggtgta ctgccttcca gacgaacgaa
     4621 gagcgattga ggaaaaggcg gcggcggccg gcatgagcct gtcggcctac ctgctggccg
     4681 tcggccaggg ctacaaaatc acgggcgtcg tggactatga gcacgtccgc gagctggccc
     4741 gcatcaatgg cgacctgggc cgcctgggcg gcctgctgaa actctggctc accgacgacc
     4801 cgcgcacggc gcggttcggt gatgccacga tcctcgccct gctggcgaag atcgaagaga
     4861 agcaggacga gcttggcaag gtcatgatgg gcgtggtccg cccgagggca gagccatgac
     4921 ttttttagcc gctaaaacgg ccggggggtg cgcgtgattg ccaagcacgt ccccatgcgc
     4981 tccatcaaga agagcgactt cgcggagctg gtattcgtgc agggcaagat tcggaatacc
     5041 aagtacgaga aggacggcca gacggtctac gggaccgact tcattgccga taaggtggat
     5101 tatctggaca ccaaggcacc aggcgggtca aatcaggaat aagggcacat tgccccggcg
     5161 tgagtcgggg caatcccgca aggagggtga atgaatcgga cgtttgaccg gaaggcatac
     5221 aggcaagaac tgatcgacgc ggggttttcc gccgaggatg ccgaaaccat cgcaagccgc
     5281 accgtcatgc gtgcgccccg cgaaaccttc cagtccgtcg gctcgatggt ccagcaagct
     5341 acggccaaga tcgagcgcga cagcgtgcaa ctggctcccc ctgccctgcc cgcgccatcg
     5401 gccgccgtgg agcgttcgcg tcgtctcgaa caggaggcgg caggtttggc gaagtcgatg
     5461 accatcgaca cgcgaggaac tatgacgacc aagaagcgaa aaaccgccgg cgaggacctg
     5521 gcaaaacagg tcagcgaggc caagcaggcc gcgttgctga aacacacgaa gcagcagatc
     5581 aaggaaatgc agctttcctt gttcgatatt gcgccgtggc cggacacgat gcgagcgatg
     5641 ccaaacgaca cggcccgctc tgccctgttc accacgcgca acaagaaaat cccgcgcgag
     5701 gcgctgcaaa acaaggtcat tttccacgtc aacaaggacg tgaagatcac ctacaccggc
     5761 gtcgagctgc gggccgacga tgacgaactg gtgtggcagc aggtgttgga gtacgcgaag
     5821 cgcaccccta tcggcgagcc gatcaccttc acgttctacg agctttgcca ggacctgggc
     5881 tggtcgatca atggccggta ttacacgaag gccgaggaat gcctgtcgcg cctacaggcg
     5941 acggcgatgg gcttcacgtc cgaccgcgtt gggcacctgg aatcggtgtc gctgctgcac
     6001 cgcttccgcg tcctggaccg tggcaagaaa acgtcccgtt gccaggtcct gatcgacgag
     6061 gaaatcgtcg tgctgtttgc tggcgaccac tacacgaaat tcatatggga gaagtaccgc
     6121 aagctgtcgc cgacggcccg acggatgttc gactatttca gctcgcaccg ggagccgtac
     6181 ccgctcaagc tggaaacctt ccgcctcatg tgcggatcgg attccacccg cgtgaagaag
     6241 tggcgcgagc aggtcggcga agcctgcgaa gagttgcgag gcagcggcct ggtggaacac
     6301 gcctgggtca atgatgacct ggtgcattgc aaacgctagg gccttgtggg gtcagttccg
     6361 gctgggggtt cagcagccag cgctttactg gcatttcagg aacaagcggg cactgctcga
     6421 cgcacttgct tcgctcagta tcgctcggga cgcacggcgc gctctacgaa ctgccgataa
     6481 acagaggatt aaaattgaca attgtgatta aggctcagat tcgacggctt ggagcggccg
     6541 acgtgcagga tttccgcgag atccgattgt cggccctgaa gaaagctcca gagatgttcg
     6601 ggtccgttta cgagcacgag gagaaaaagc ccatggaggc gttcgctgaa cggttgcgag
     6661 atgccgtggc attcggcgcc tacatcgacg gcgagatcat tgggctgtcg gtcttcaaac
     6721 aggaggacgg ccccaaggac gctcacaagg cgcatctgtc cggcgttttc gtggagcccg
     6781 aacagcgagg ccgaggggtc gccggtatgc tgctgcgggc gttgccggcg ggtttattgc
     6841 tcgtgatgat cgtccgacag attccaacgg gaatctggtg gatgcgcatc ttcatcctcg
     6901 gcgcacttaa tatttcgcta ttctggagct tgttgtttat ttcggtctac cgcctgccgg
     6961 gcggggtcgc ggcgacggta ggcgctgtgc agccgctgat ggtcgtgttc atctctgccg
     7021 ctctgctagg tagcccgata cgattgatgg cggtcctggg ggctatttgc ggaactgcgg
     7081 gcgtggcgct gttggtgttg acaccaaacg cagcgctaga tcctgtcggc gtcgcagcgg
     7141 gcctggcggg ggcggtttcc atggcgttcg gaaccgtgct gacccgcaag tggcaacctc
     7201 ccgtgcctct gctcaccttt accgcctggc aactggcggc cggaggactt ctgctcgttc
     7261 cagtagcttt agtgtttgat ccgccaatcc cgatgcctac aggaaccaat gttctcggcc
     7321 tggcgtggct cggcctgatc ggagcgggtt taacctactt cctttggttc cgggggatct
     7381 cgcgactcga acctacagtt gtttccttac tgggctttct cagccgggat ggcgctaaga
     7441 agctattgcc gccgatcttc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     7501 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     7561 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     7621 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     7681 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     7741 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     7801 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     7861 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc
     7921 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     7981 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     8041 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     8101 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     8161 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     8221 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     8281 atatcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     8341 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     8401 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     8461 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     8521 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     8581 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     8641 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     8701 tattaaacaa gtggcagcaa cggattcgca aacctgtcac gccttttgtg ccaaaagccg
     8761 cgccaggttt gcgatccgct gtgccaggcg ttaggcgtca tatgaagatt tcggtgatcc
     8821 ctgagcaggt ggcggaaaca ttggatgctg agaaccattt cattgttcgt gaagtgttcg
     8881 atgtgcacct atccgaccaa ggctttgaac tatctaccag aagtgtgagc ccctaccgga
     8941 aggattacat ctcggatgat gactctgatg aagactctgc ttgctatggc gcattcatcg
     9001 accaagagct tgtcgggaag attgaactca actcaacatg gaacgatcta gcctctatcg
     9061 aacacattgt tgtgtcgcac acgcaccgag gcaaaggagt cgcgcacagt ctcatcgaat
     9121 ttgcgaaaaa gtgggcacta agcagacagc tccttggcat acgattagag acacaaacga
     9181 acaatgtacc tgcctgcaat ttgtacgcaa aatgtggctt tactctcggc ggcattgacc
     9241 tgttcacgta taaaactaga cctcaagtct cgaacgaaac agcgatgtac tggtactggt
     9301 tctcgggagc acaggatgac gcctaacaat tcattcaagc cgacaccgct tcgcggcgcg
     9361 gcttaattca ggagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac
     9421 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     9481 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     9541 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     9601 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     9661 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     9721 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     9781 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     9841 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     9901 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     9961 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
    10021 tggagcgcct gccggcccag tatcagcccg tcatacttga agctaggcag gcttatcttg
    10081 gacaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtt cactacgtga
    10141 aaggcgagat caccaaggta gtcggcaaat aatgtctaac aattcgttca agccgacgcc
    10201 gcttcgcggc gcggcttaac tcaagcgtta gagagctggg gaagactatg cgcgatctgt
    10261 tgaaggtggt tctaagcctc gtacttgcga tggcatcggg gcaggcactt gctgacctgc
    10321 caattgtttt agtggatgaa gctcgtcttc cctatgacta ctccccatcc aactacgaca
    10381 tttctccaag caactacgac aactccataa gcaattacga caatagtcca tcaaattacg
    10441 acaactctga gagcaactac gataatagtt catccaatta cgacaatagt cgcaacggaa
    10501 atcgtaggct tatatatagc gcaaatgggt ctcgcacttt cgccggctac tacgtcattg
    10561 ccaacaatgg gacaacgaac ttcttttcca catctggcaa aaggatgttc tacaccccaa
    10621 aaggggggcg cggcgtctat ggcggcaaag atgggagctt ctgcggggca ttggtcgtca
    10681 taaatggcca attttcgctt gccctgacag ataacggcct gaagatcatg tatctaagca
    10741 actagcctgc tctctaataa aatgttagga gcttggctgc catttttggg gtgaggccgt
    10801 tcgcggccga ggggcgcagc ccctgggggg atgggaggcc cgcgttagcg ggccgggagg
    10861 gttcgagaag ggggggcacc ccccttcggc gtgcgcggtc acgcgccagg gcgcagccct
    10921 ggttaaaaac aaggtttata aatattggtt taaaagcagg ttaaaagaca ggttagcggt
    10981 ggccgaaaaa cgggcggaaa cccttgcaaa tgctggattt tctgcctgtg gacagcccct
    11041 caaatgtcaa taggtgcgcc cctcatctgt cagcactctg cccctcaagt gtcaaggatc
    11101 gcgcccctca tctgtcagta gtcgcgcccc tcaagtgtca ataccgcagg gcacttatcc
    11161 ccaggcttgt ccacatcatc tgtgggaaac tcgcgtaaaa tcaggcgttt tcgccgattt
    11221 gcgaggctgg ccagctccac gtcgccggcc gaaatcgagc ctgcccctca tctgtcaacg
    11281 ccgcgccggg tgagtcggcc cctcaagtgt caacgtccgc ccctcatctg tcagtgaggg
    11341 ccaagttttc cgcgaggtat ccacaacgcc ggcggccggc cgcggtgtct cgcacacggc
    11401 ttcgacggcg tttctggcgc gtttgcaggg ccatagacgg ccgccagccc agcggcgagg
    11461 gcaaccagcc cggtgagcgt cggaaaggg
//
