LOCUS       V009110                10755 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009110
VERSION     V009110
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10755)
  AUTHORS   Bauler LD, Hackstadt T.
  TITLE     Expression and Targeting of Secreted Proteins from Chlamydia
            trachomatis
  JOURNAL   J. Bacteriol. 196 (7), 1325-1334 (2014)
   PUBMED   24443531
REFERENCE   2  (bases 1 to 10755)
  AUTHORS   Bauler LD, Hackstadt T.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2013) Laboratory of Intracellular Parasites, Rocky
            Mountain Laboratories, NIAID, NIH, 903 S. Fourth St., Hamilton, MT
            59840, USA
REFERENCE   3  (bases 1 to 10755)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10755)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Bacteriol."; date: "2014"; volume: "196"; issue: "7"; pages:
            "1325-1334"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-OCT-2013) Laboratory of Intracellular Parasites, Rocky Mountain
            Laboratories, NIAID, NIH, 903 S. Fourth St., Hamilton, MT 59840,
            USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10755
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(5055..6407)
                     /note="Probable plasmid replicative DNA helicase from
                     Chlamydia trachomatis serovar L2 (strain 434/Bu / ATCC
                     VR-902B). Accession#: B0BCM3"
                     /label="Probable plasmid replicative DNA helicase"
     regulatory      7555..7742
                     /gene="rpoB"
                     /regulatory_class="promoter"
     gene            7555..7742
                     /gene="rpoB"
                     /label="rpoB"
     misc_feature    7743..7777
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     promoter        7778..7849
                     /label="AmpR promoter"
     misc_feature    8034..8413
                     /label="ISS"
                     /note="immunostimulatory sequence from the AmpR gene;
                     contains unmethylated CpG dinucleotides in the context of
                     5'-AACGTT-3' (Sato et al., 1996)"
     rep_origin      8881..9468
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 ggtgtttttt ctggcaacag aatatgaata taattttaat tatatcacaa tattgggggt
       61 gtttgtacta gaggacttac ctcttcccca gaacaataag aacacacact ttgtctcgat
      121 gaaagacagg aaatacgcat gatttcctca tcttttaatc ctatttgctt taaatgaatc
      181 aaagcgcttg cacgaagtac tctaggagta attttttttt tcatagcact atagaactct
      241 gcaagcctaa aattatgcgc aacctgactt gttgttacag gaatccctat tttagaaaca
      301 aatactctcc catttctccc acaagtgtat tttttgcaac tcctccatta agctgatagg
      361 aaatgtgatt agaattttgg tttctttatt ctgtcttttt ttaatgcgaa aggaaatctg
      421 attggatgca aaaaatagat cggtctgtgc gcaaagacaa aatttcgtct aacttacgga
      481 taccttgtac aatcaattta ccgattaaat agtctctata attcactatc cggagcgctt
      541 caaaaaaaac tgtccattcc tgcttagaaa tcgattctgt tttgattttg tctcggattt
      601 taaaaaatgt agtgtttcca aaatctttca atggaatagc gggtttaata tatcccttgg
      661 tcaatctata caaaaacttt gtgaaagata tgtagcatgc cgctctagcc tgtttagagg
      721 cctctgaaac gacttttcca ttaaaaacat ctagagactt gattttaaac aaagattcgc
      781 tgtggtcaag agaaatagcc tttatcaagg tttccgataa atccagaatc tctaaagaaa
      841 caagaaagtt aatcccagac gcataatttt ttctagttag atgagataaa gtagataacc
      901 aaatttccga cgcgtcccca aaagttaaga acaacctact tttatggaaa gccatcgagc
      961 ccattttctt aaccaaagct attcaaaatc ggagctctaa gattttaaga aatttttcaa
     1021 caaaagtcca ttatgaccaa gtctaccacc aagagttgca aagtctacca ccaagagttg
     1081 caaagtctac caccaagagt tgcaaagtct accaccaaga gttgcaaatc tctctcgtaa
     1141 aatcaaatcc ctaaatatat atatataata gatatatata tgagctgacg gaggatcagc
     1201 tcttttgctt aaaaagttca aaaagctgtt gtagaagatt ttcgttatag gaggacaaag
     1261 aaactccgga acacatgatg cgaagtatct ctattaagaa atcagataat tggcgattct
     1321 tctctgaatc agacttatct atcgtttctc taacgtcttt gtttctagat gaaggaagaa
     1381 attgatccaa cacccttatc gccgatgagt tcgacattcc acatactttc cctatcacat
     1441 cgaccttggt ttttaaatcg ccttttctag cggccaaaat atatgcggat ttatagggga
     1501 tcgattgaaa ctctttttgt agagtttggt tggggaggtt tataaaaagc tcgtaatatg
     1561 caagagcatt gtaagcagaa gacttagttc taaaaactaa ctctatccaa gatgaaaaag
     1621 ttgttgagga gaagtgatcc ttactcagga tttttctagc attatagatt ttttctccta
     1681 aaagaagtac gtgttgcttc tgtatggatt ttatctgacc agtaagcagt tttaccgcta
     1741 ggatgtcttc ttgataaaat tcttcatccg aatagttttg ggactctgat aaaaataatt
     1801 gatccaaact ctgactttcc tcagaattca aagttgctga gaatagttca atggaaggaa
     1861 gcgtcttctt aaaatctaaa gaagcggcag tttgattttt tttaaaaaag acattcgctt
     1921 ctttttttag tttgttcacg ttgtcctctg agagtaatct cgttcatatt cgatatgcaa
     1981 aatatttgct atttcatgcg ttaacttcag aatatcttct gcggccctag aatttggata
     2041 gacattagct acagaatctt ctttaagaag agaacggctg agagaaatat ctcgacgaat
     2101 ttttgttgaa aaaagcttgt ttttgtaaat agactcgata atgtctatat acatttggtt
     2161 agtcgagtta cgatcatccc aaaaagacaa agctattcca agaatgtgtt cttcttcagg
     2221 ttttccgacc gaacttaaga attcacgtat cttttgtaac cctagaatag aaaaaggttc
     2281 tggagttaaa caagcaatta atttgtctcc tgcaacaaaa gcttctttcg ttaaccctcc
     2341 taggctaggt ggagtgtcta ttatgcagat gtcataaaaa ggagcgcagt actcattcag
     2401 aaataacttt aagttgttac taggtcctct atgaatatcc aattctctaa actgttcgga
     2461 taaaaatgat gcaggaatta ggtccacact atcttttttt gtttcgcaaa tgattgattt
     2521 taaatcgttt gatgtgtata ctatgtcgtg taagcctttt tggttacttc tgacactagc
     2581 ccccaatcca gaagataaat tggattgcgg gtctaggtca gcaagtaaca cttttttccc
     2641 taaaaattgg gccaagttgc atcccacgtt tagagaaagt gttgtttttc cagttcctcc
     2701 cttaaaagag caaaaaacta aggtgtgcaa atcaactcca acgttagagt aagttatcta
     2761 ttcagccttg gaaaacatgt cttttctaga caagataagc ataatcaaag ccttttttag
     2821 ctttaaactg ttatcctcta atttttcaag aacaggagag tctgggaata atcctaaaga
     2881 gttttctatt tgttgaagca gtcctagaat tagtgagaca cttttatggt agagttctaa
     2941 gggagaattt aagaaagtta ctttttcctt gtttactcgt atttttaggt ctaattcggg
     3001 gaaatctttt ttcacatctt taacaatttt aataaaatcg tccctcactt tgcttttatt
     3061 ttgcataaca aaccccgtaa ttcgaacttt tttctctaaa tataaaacct ataagaaaaa
     3121 tccaataaaa attgtttaag cgtttgtttg aggtattacc tccaaaaaag atacattaga
     3181 agtatttgtt attcctaaaa tatcattgcc attagaaagg gcattaaccc ataccacacc
     3241 gctttctaaa ccgcctacac gtaatgaata cgttgtcgga gtcaatcctg tattaataat
     3301 tctggttctt agactacata aattaggaac gcctgatgag tatccataac taatcgcgta
     3361 gggcttagaa tcaccttctc gtaccaaagc tagaacaacg ccgccttcca ttcttgatgc
     3421 aataatatct gctgagacta agaacatgct cccagagctt ttgggtgtga ctgtgaattt
     3481 tcctatttca gttcctccta ataaagtttc aatgttcctg ggagtgaata acccgttgca
     3541 ttgaatttta ttagtgattg gaaagttgtt aaaagctttc aacaaaccta gagaagggtc
     3601 tgttgtgatt ttgtctaaaa tatcttggac tgtactatca acaatagtat cagcaattcc
     3661 accaagaatt tgatctccca acttttctag aataagctgg taagcttttt ccgcatccaa
     3721 accaattgta atagaagcat tgggttgatg gattattgga gactgttaaa gatattccat
     3781 cagaagctgt cattttggct gcgacaggtg ttgatgttgt cccaaggatt atttgctggt
     3841 ccttgagcgg ctctgtcatt tgcccaactt tgatattatc agcaaagacg cagttttgag
     3901 tgttatacaa ataaaaacca gaatttccca ttttaaaact cttttttatt ttgagcttta
     3961 aataaattag gtttttagtt tcaagtttgc tattaattaa tagattcttg ttctaattgt
     4021 tccatttgtt ctttagattt cttagttatt tcttcaaaac gctctttatt tagatataga
     4081 atttcttttt tagagagttt agaagaatcc agaaattcaa tgcgttttct tctagataac
     4141 caaccaagct gaatggcgat ttctatacat ttatcgatag ctaactcgat ttttttccag
     4201 ttccttgtac aaatgtaccg attcatcctt aaaatatatg caagactttt aacgttaacg
     4261 tttaagaaca agttttctgg ccaagaatta tccttagtta attttcgtct ctttttcgca
     4321 gctgctgtaa tcacccagtc gataaatgtg taagcatact ttgatgcatt tgggaagcgc
     4381 atttttattt cttggtatac atttgcaggc ttgattacaa agtaggattc tatttgatct
     4441 accaagatag gacatggctc tacaacgaac cctttatgtt tccgtgtaga tggtgaatta
     4501 aaaggtgtta agtctatatc tatattttct tcgtcagtta aaccttccca tccttcgtaa
     4561 atcctaatga tcggagaaag agtttgggta acggtctact atttgtgttc cattagtcca
     4621 tcgagttcta gttgccacta ttaaaaacgg ttgatgtcct aaatggtata aggcttctaa
     4681 agcagtttca gcttcttttc cactaaactc atacttattt ctggatgttt tataccgctt
     4741 aactccataa gcctctaaga attcagtttt tgtaaaacgg attcttggta tccatccttc
     4801 aaattgaaaa ctatttgatt ctctggataa aacaacccct tttgtgttcc ccttgtaatt
     4861 cgttgcagtc agcaatcttt ggatagctgc taatgcatgg taatgagatg aaagaaaatc
     4921 aagacctata acttctacca tcccattttg agccaatttg ggagatatct taatagattg
     4981 accaggtctt cttccaaact tctgattttc aaggtggata ggacttttga tgaagtggca
     5041 gttactataa tttaccatac ttttttaata gcggagaatt tactaatttt tggatcgaaa
     5101 tgtaataccg aagagaaaac cgatccatgt ctatttttcc caacagttat ctcacaatta
     5161 gaagacgatt ccttcctatt gataaacaaa atcacatctg cgtcttgctc tatttgaccg
     5221 ctgtctcgca aatctgaaag catgggaact ttatttgctc tatcctcaac ttttctagat
     5281 agttgggata aacaaactat aggaatgttt agctctgagg ctaaacctct taaggttcta
     5341 gatatatctg ctatttcatt ttgacgattt tctccaaccg atgagttgat caactgcaag
     5401 taatcgataa atattacgtc tactcgatct ycttttctca gcaaccggat ctgattcgcg
     5461 attaaattaa gcttatactg actatcacta cagatataaa aatgtgattc tctaactgtt
     5521 tctccagctt cttctactcg gaataattct tctttagaga gatcccctct ttgtaatttt
     5581 tcaccagata ttcctgttaa attagcaata atccgctcaa caatttgacc tgcgctcatt
     5641 tctagagata ggaaaccaac tctacgctgt tgagtaaccg caagatttat cgccatgtct
     5701 atagctaaag cagttttccc tatagatggc ctagctgcta taatcacgaa attaccttta
     5761 gctaagataa ctcctttatc atcaatatcc ttgtatcctg ttgggaaggc atcaaagaaa
     5821 gaattttgat tctcagagaa cgttgctcgt ctttttttta tacgagccag cactccaatt
     5881 tctgactgtg agaatatatc ataaatagac cggcctctag cgctgcgaat agaaaaagtc
     5941 tttgctatag cactgtcaag ccttcccttt atacgctcaa gcaatagaaa cggagatcta
     6001 cgcaatggat tttcattgta ctcattaaac gagcggaaaa tgaaattact caaattttct
     6061 tcagcgctac acacgctcaa atcatcgagg aaaaccgtat gagaaacgga tctaagcttg
     6121 tcatttgata aagcatcatg caacattaac ccgagatacg atttgtccat atctttgata
     6181 cgacgccgca aaagctcttc ccaagccgag tctacagtta taggtaatcc attgtctttt
     6241 aagtatttaa atactatgaa tatgttttta tgatgagaac acttaaactc ataattagca
     6301 agctgcctca gaatatactc agtagagtct tcaaatatca gagctttacc taacaacgca
     6361 tactcgatat cttgcatgcg attttctatt tcggaacgag ttttcatgtt tatataaaaa
     6421 aataccgagc gtgctatcct gttaacaacc tgattatttc actaatcagg acattttacg
     6481 gataggttat atcacgaggg atttcatggg taaagggatt ttatctttgc agcaagaaat
     6541 gtcgttagaa tatagcgaaa agtcttatca ggaagtttta aaaattcgcc aagaatccta
     6601 ttggaaacgc atgaaaagct tctccttatt cgaagttatt atgcattgga ccgcatcact
     6661 caacaaacat acttgtagat catatcgagg atcttttttg tctttagaaa agattggtct
     6721 attgtccttg gatatgaatc tgcaagagtt ttccctttta aatcataatc taattctaga
     6781 tgcgattaaa aaagtttcct ctgccaagac ttcttggacc gaaggtacta aacaagttcg
     6841 agcagcaagc tatatttcct taacaagatt cctaaacagg atgactcaag gaatagtcgc
     6901 tatagcgcaa ccttctaaac aagaaaatag tcgaacattt tttaaaacca gggaaatagt
     6961 aaaaacagat gcgatgaaca gtttgcaaac agcgtccttc ctaaaagagc taaaaaaaat
     7021 caatgcccgg gattggttga tcgcccagac aatgctccaa ggaggtaaac gctcctctga
     7081 agtcttaagc ttggagatta gtcagatttg tttccaacaa gctaccattt ctttctccca
     7141 gcttaagaac cgtcagacag aaaagaggat tattataact tatcctcaga agtttatgca
     7201 ctttctacaa gagtacatcg gtcaacgaag aggttttgtc ttcgtaactc gctccggaaa
     7261 aatggtgggg ttaaggcaaa tcgcccgcac gttctctcaa gcaggactac aagctgcaat
     7321 cccttttaag ataaccccgc acgtgcttcg agcaaccgct gtgacggagt acaaacgcct
     7381 agggtgctca gactccgaca taatgaaggt cacaggacac gcaaccgcaa agatgatatt
     7441 tgcgtacgat aaatcttctc gagaagacaa cgcttcaaag aagatggctc taatatagcc
     7501 taaaagtgtt ttttctggca acagaatatg aatataattt taattatatc accttaatgg
     7561 aatcaaggtg atagtatttg tcggatggac aagtgtatag ggagtatcca gtgtctctgt
     7621 attggatagg ctctgttttg tcctagctgg aaagcatctg tcgtattcct gtttagagat
     7681 cacagaggga ctaaataggg aaatggtatc gccaaaagtc ttaaagtctt aggagagctc
     7741 gcggatccgc ggccgctagc tgcaggtacc ggtcgaccat tcaaatatgt atccgctcat
     7801 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     7861 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     7921 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     7981 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     8041 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     8101 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg gttgagtact
     8161 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     8221 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     8281 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     8341 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     8401 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     8461 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     8521 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     8581 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     8641 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     8701 agcattggta actgtcagac caagtttact catatatact ttagatttga tttaaaactt
     8761 catttttaat ttaaaaggat ctaggtgaag atcctttttg atatctcatg accaaaatcc
     8821 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaagatca aaggatcttc
     8881 ttgagatcct tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     8941 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     9001 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     9061 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     9121 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     9181 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     9241 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     9301 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     9361 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     9421 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa tgccagcaac
     9481 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgga attcgatgcc
     9541 cgacggtctt tatagcggat taacaaaaat caggacaagg cggcgaagcc gaagacagta
     9601 caaatagcac ggaaccgatt cacttggtgc ttcagcacct tagagaatcg ttctctttga
     9661 gctaaggcga ggcaacgccg tacttgtttt tgttaatcca ctataaagtg ccgcgtgtgt
     9721 ttttttatgg cgttttaaaa agccgagact gcatccgggc agcagcgcat cggcccgcac
     9781 gaggtctgcg cttgaattgt gttgtagaaa cacaacgttt tttgaaaaaa taagctattg
     9841 ttttatatca aaatataatc atttttaaaa taaaggttgc ggcatttatc agatatttgt
     9901 tctgaaaaat ggttttttgc gggggggggg gtataattga agacgtatcg ggtgtttgcc
     9961 cgatgttttt aggtttttat caaatttaca aaaggaagcc gatctagatg agtaaaggag
    10021 aagcactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc
    10081 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta
    10141 aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc actactctta
    10201 cgtatggtgt tcaatgcttt tcaagatacc cagatcatat gaaacggcat gactttttca
    10261 agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga
    10321 actacaagac agtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt
    10381 aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta
    10441 taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt
    10501 caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa
    10561 tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc
    10621 tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac
    10681 agctgctggg attacacatg gcatggatga actatacaag taacgatgag tggcagggcg
    10741 gggcgtaagc ctaaa
//
