LOCUS       40924_6926        7135 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Broad host range vector pBMTBX-6, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7135)
  AUTHORS   Prior JE, Lynch MD, Gill RT.
  TITLE     Broad host range vectors for expression in Gram negative hosts
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7135)
  AUTHORS   Prior JE, Lynch MD, Gill RT.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2009) Chemical and Biological Engineering, 
            University of Colorado, UCB 424, Boulder, CO 80309, USA
REFERENCE   3  (bases 1 to 7135)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7135)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-AUG-2009) Chemical and Biological Engineering, University of 
            Colorado, UCB 424, Boulder, CO 80309, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7135
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(60..96)
                     /label=soxR terminator
                     /note="bidirectional E. coli soxR transcription terminator"
     CDS             complement(104..1123)
                     /codon_start=1
                     /gene="mob"
                     /product="Mob"
                     /label=mob
                     /note="required for mobilization"
                     /protein_id="ACV83895.1"
                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEHW
                     AASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKWLA
                     DKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAAAVA
                     DLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLGISKR
                     VETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVLDALGP
                     LNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSKKPLLS"
     gene            complement(104..1123)
                     /gene="mob"
                     /label=mob
                     /note="mob gene from pBBR1MCS in INSD accession U02374"
     rep_origin      1347..2116
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             2117..2776
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     CDS             2876..3649
                     /codon_start=1
                     /gene="kilA"
                     /product="KilA"
                     /label=kilA
                     /note="involved in tellurite resistance"
                     /protein_id="ACV83897.1"
                     /translation="MEEQSVNMARLKGEVLPALFASPATIGEYGAGIDGADSLNELSNL
                     MEHGAVAALADKISQIVAKLADADPRKIAEKPTWFEKMLGREVERQVRYQVARKTLDQL
                     LDEAEGVAQRVRDTLRALDDMLNTHEAEVDRLRAYIQAGREFLDENPEAGAAKAGVIEF
                     DKPRERFARKLANLATLMASHEMSVTQMKLTRAQAVDMLDRFSETASVLVPVWRQHTLA
                     LITTKNMNPAMVAEAAKAHQALMRSLSQSLEGINQ"
     gene            2876..3649
                     /gene="kilA"
                     /label=kilA
     CDS             3667..4803
                     /codon_start=1
                     /gene="telA"
                     /product="TelA"
                     /label=telA
                     /note="involved in tellurite resistance"
                     /protein_id="ACV83898.1"
                     /translation="MNALKTTHDAKAPIVAFDMTPATLRELGLQESDVPEVHAVAQRIE
                     VGSPQTVAEFGRDVAEHTSRYADSLLDQVRNSDLDEAGEKLTQVVAKARSLNVGPLSDN
                     RSRLPLIGPLIDRFRVRSTGFMARFDTTREQIEHLVSEVQTTQQGIAQRNASLDEMFAA
                     VREEHRLLGVHIAAGKVRLAELREQAEGLRGNVGNDPGRVQELADLDAMVANLDKRIGD
                     LIALQHSAMQSLPTIRMIQANNQMLVDKFHTIREITVPAWKRQFMLALSLNEQKNAVEL
                     ATAIDDTTNDLMKRNAALLHRTSVETAKENQRLVIDVDTLKQVQTTLIKTVEDVIRIQQ
                     EGVQKRKDAEKQIAAMRGDLQAKLTRQPVRELAQQESV"
     gene            3667..4803
                     /gene="telA"
                     /label=telA
     CDS             4800..5753
                     /codon_start=1
                     /gene="telB"
                     /product="TelB"
                     /label=telB
                     /note="involved in tellurite resistance"
                     /protein_id="ACV83899.1"
                     /translation="MNATNTDVFAQVGGLEARGAKMKKRGTRFLIAALAVLAIAGIGAV
                     TGWAISPSATPGSIDVPQVLASTFSDQVPGSEGGGLGGGLPFTSAVGAFTDFMAGPAIF
                     TLGILGIVVAGAVLVFGGEFCGFVRSVCMMVIAVSMIFVSSNLVKGILGGDHDAGPAEP
                     SPRARFMAAVEAKDFARVQELIEARGAKSAADYVLAQLAVAEGLDRKPGARVVVGKAAG
                     SMAMPPAALGFTPRGEAAYAIERSAYGEPRSSIAKQYQQEWNRKAATWWAMAGVAGIIG
                     AILAAAATGFVGLAVSIRNRVKRVRDLLVMEPGAEP"
     gene            4800..5753
                     /gene="telB"
                     /label=telB
     terminator      complement(5767..5798)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     CDS             complement(5829..6704)
                     /codon_start=1
                     /label=araC
                     /note="L-arabinose regulatory protein"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        6731..7015
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
ORIGIN
        1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga
       61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgctcatgat aataatggtt
      121 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg cccgcgttcc tgctggcgct
      181 gggcctgttt ctggcgctgg acttcccgct gttccgtcag cagcttttcg cccacggcct
      241 tgatgatcgc ggcggccttg gcctgcatat cccgattcaa cggccccagg gcgtccagaa
      301 cgggcttcag gcgctcccga aggtctcggg ccgtctcttg ggcttgatcg gccttcttgc
      361 gcatctcacg cgctcctgcg gcggcctgta gggcaggctc atacccctgc cgaaccgctt
      421 ttgtcagccg gtcggccacg gcttccggcg tctcaacgcg ctttgagatt cccagctttt
      481 cggccaatcc ctgcggtgca taggcgcgtg gctcgaccgc ttgcgggctg atggtgacgt
      541 ggcccactgg tggccgctcc agggcctcgt agaacgcctg aatgcgcgtg tgacgtgcct
      601 tgctgccctc gatgccccgt tgcagcccta gatcggccac agcggccgca aacgtggtct
      661 ggtcgcgggt catctgcgct ttgttgccga tgaactcctt ggccgacagc ctgccgtcct
      721 gcgtcagcgg caccacgaac gcggtcatgt gcgggctggt ttcgtcacgg tggatgctgg
      781 ccgtcacgat gcgatccgcc ccgtacttgt ccgccagcca cttgtgcgcc ttctcgaaga
      841 acgccgcctg ctgttcttgg ctggccgact tccaccattc cgggctggcc gtcatgacgt
      901 actcgaccgc caacacagcg tccttgcgcc gcttctctgg cagcaactcg cgcagtcggc
      961 ccatcgcttc atcggtgctg ctggccgccc agtgctcgtt ctctggcgtc ctgctggcgt
     1021 cagcgttggg cgtctcgcgc tcgcggtagg cgtgcttgag actggccgcc acgttgccca
     1081 ttttcgccag cttcttgcat cgcatgatcg cgtatgccgc catgcctgcc cctccctttt
     1141 ggtgtccaac cggctcgacg ggggcagcgc aaggcggtgc ctccggcggg ccactcaatg
     1201 cttgagtata ctcactagac tttgcttcgc aaagtcgtga ccgcctacgg cggctgcggc
     1261 gccctacggg cttgctctcc gggcttcgcc ctgcgcggtc gctgcgctcc cttgccagcc
     1321 cgtggatatg tggacgatgg ccgcgagcgg ccaccggctg gctcgcttcg ctcggcccgt
     1381 ggacaaccct gctggacaag ctgatggaca ggctgcgcct gcccacgagc ttgaccacag
     1441 ggattgccca ccggctaccc agccttcgac cacataccca ccggctccaa ctgcgcggcc
     1501 tgcggccttg ccccatcaat ttttttaatt ttctctgggg aaaagcctcc ggcctgcggc
     1561 ctgcgcgctt cgcttgccgg ttggacacca agtggaaggc gggtcaaggc tcgcgcagcg
     1621 accgcgcagc ggcttggcct tgacgcgcct ggaacgaccc aagcctatgc gagtgggggc
     1681 agtcgaaggc gaagcccgcc cgcctgcccc ccgagcctca cggcggcgag tgcgggggtt
     1741 ccaagggggc agcgccacct tgggcaaggc cgaaggccgc gcagtcgatc aacaagcccc
     1801 ggaggggcca ctttttgccg gagggggagc cgcgccgaag gcgtggggga accccgcagg
     1861 ggtgcccttc tttgggcacc aaagaactag atatagggcg aaatgcgaaa gacttaaaaa
     1921 tcaacaactt aaaaaagggg ggtacgcaac agctcattgc ggcacccccc gcaatagctc
     1981 attgcgtagg ttaaagaaaa tctgtaattg actgccactt ttacgcaacg cataattgtt
     2041 gtcgcgctgc cgaaaagttg cagctgattg cgcatggtgc cgcaaccgtg cggcacccta
     2101 ccgcatggag ataagcatgg ccacgcagtc cagagaaatc ggcattcaag ccaagaacaa
     2161 gcccggtcac tgggtgcaaa cggaacgcaa agcgcatgag gcgtgggccg ggcttattgc
     2221 gaggaaaccc acggcggcaa tgctgctgca tcacctcgtg gcgcagatgg gccaccagaa
     2281 cgccgtggtg gtcagccaga agacactttc caagctcatc ggacgttctt tgcggacggt
     2341 ccaatacgca gtcaaggact tggtggccga gcgctggatc tccgtcgtga agctcaacgg
     2401 ccccggcacc gtgtcggcct acgtggtcaa tgaccgcgtg gcgtggggcc agccccgcga
     2461 ccagttgcgc ctgtcggtgt tcagtgccgc cgtggtggtt gatcacgacg accaggacga
     2521 atcgctgttg gggcatggcg acctgcgccg catcccgacc ctgtatccgg gcgagcagca
     2581 actaccgacc ggccccggcg aggagccgcc cagccagccc ggcattccgg gcatggaacc
     2641 agacctgcca gccttgaccg aaacggagga atgggaacgg cgcgggcagc agcgcctgcc
     2701 gatgcccgat gagccgtgtt ttctggacga tggcgagccg ttggagccgc cgacacgggt
     2761 cacgctgccg cgccggtagt acgtaagagg ttccaacttt caccataatg aaataagatc
     2821 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga
     2881 agaacaaagc gtgaacatgg cgcgattgaa gggggaggtt ttgcccgccc tcttcgcgtc
     2941 gccggcgacg attggcgagt acggggccgg catcgacggg gcggattccc tcaacgagct
     3001 gtcgaatctg atggagcacg gcgcagttgc cgcgctggcc gacaaaatca gccagatcgt
     3061 ggcgaagctg gccgacgcgg acccccgcaa gatcgcggaa aagcctacct ggttcgagaa
     3121 gatgcttggc cgtgaggttg aacgccaggt gaggtatcag gtcgcccgca agacgctcga
     3181 ccagttgctg gacgaagccg agggcgtagc gcagcgcgtg cgggacacgt tgcgcgcctt
     3241 ggatgacatg ctcaatacgc atgaggccga ggtagaccgg ctcagagcct acattcaagc
     3301 cgggcgcgag ttcctggacg agaaccccga ggccggcgcg gccaaggccg gcgtgatcga
     3361 gttcgacaag ccgcgcgaac gcttcgcgcg caagctcgcc aacctggcaa ccctcatggc
     3421 gtcccatgaa atgagcgtca ctcagatgaa gctcacgcgg gcgcaggccg tggacatgct
     3481 ggaccgcttc tctgaaacgg catccgtcct ggtgcccgtc tggcgtcagc acaccctcgc
     3541 gctcatcacc accaagaaca tgaatccggc aatggtcgcc gaggcggcca aagctcacca
     3601 ggcgctcatg cggagccttt cgcagagcct ggaaggcatc aaccaataac acggcgggag
     3661 aaccctatga acgcactgaa aacgacgcac gacgccaagg cccctatcgt cgccttcgac
     3721 atgaccccgg caaccctgcg cgagctgggc ttgcaggaaa gcgacgtgcc ggaagtccat
     3781 gcggtcgcgc agcggatcga ggtcggcagt ccgcagaccg ttgccgagtt cggccgcgac
     3841 gtggccgagc acacgtcccg ctacgccgat agcctgctgg accaggtgcg caacagcgac
     3901 ctggacgaag caggcgagaa actgacccag gttgtcgcca aggcccgttc cctgaacgtc
     3961 ggccctttgt ccgacaaccg ttcccgcctg cccctgattg gcccgctgat cgaccgcttc
     4021 cgcgtccgtt cgacgggctt catggcgcgc ttcgacacga cccgcgagca gatcgaacac
     4081 ctggtcagcg aagtgcagac cacccagcaa ggcatcgcgc agcgcaatgc ctcgctcgac
     4141 gaaatgttcg cagccgtgcg cgaggaacac cgccttcttg gcgtccacat cgcggccggc
     4201 aaggtccgcc ttgccgagct gcgcgagcag gccgagggtc tgcgcggcaa tgtcgggaac
     4261 gacccgggcc gcgtgcagga gctggccgac ctcgatgcga tggttgccaa cctggacaag
     4321 cgcatcggcg acctgatcgc cttgcaacat tcggccatgc agagcctgcc gaccatccgc
     4381 atgatccagg ccaacaacca gatgctggtc gataaattcc acaccatccg cgaaatcacc
     4441 gtgccggcgt ggaagcggca attcatgctg gccttgagcc tcaacgagca gaagaacgcc
     4501 gtcgaactgg ccacggccat cgacgacacc accaacgacc tgatgaagcg caatgcggcc
     4561 ctgctgcatc gcacgtccgt cgagacggcg aaggagaacc aacgcctggt gatcgacgtg
     4621 gacacgctca agcaggttca gacgacgctc atcaagaccg tcgaggacgt tattcgcatc
     4681 cagcaggaag gcgtgcagaa gcgcaaggat gccgagaagc agatcgccgc aatgcgtggc
     4741 gatcttcaag ccaagctgac ccgccagccc gtgcgcgagc tggcccaaca ggagtccgta
     4801 tgaatgccac aaacaccgat gttttcgccc aggtaggcgg cctcgaggcc cgaggcgcga
     4861 agatgaagaa gcggggcacc cgcttcctca tcgcggcgct ggcagtcctt gccattgccg
     4921 ggatcggggc agtaacggga tgggcgatca gcccgagcgc gacgcccgga agcattgacg
     4981 tgccgcaggt gctggcatcg acattcagcg accaggtgcc gggcagtgag ggcggcggcc
     5041 tgggtggcgg cctgcccttc acttcggccg tcggggcatt cacggacttc atggcggggc
     5101 cggcaatttt taccttgggc attcttggca tagtggtcgc gggtgccgtg ctcgtgttcg
     5161 ggggtgaatt ctgcgggttc gtgcgatccg tctgcatgat ggtgatagcc gtcagcatga
     5221 ttttcgtgtc gtcgaacttg gtgaagggca ttctcggcgg cgatcacgac gccggccctg
     5281 cggagccttc gccgcgtgcg cgattcatgg cggccgtgga ggccaaggat ttcgcgcgag
     5341 tgcaagagct gatcgaggcg cgtggagcca agtcggcggc tgattatgtc cttgcgcagc
     5401 tcgccgtggc cgaaggtctg gaccgcaagc ctggtgcgcg cgtcgtggtc gggaaagcgg
     5461 cgggcagcat ggcaatgccg cctgcggcgc tgggttttac gccaagggga gaagcggcat
     5521 acgccatcga gcggtcagcc tatggtgagc cgaggtccag cattgcgaag cagtaccagc
     5581 aggaatggaa ccggaaggcg gcgacctggt gggcgatggc cggtgtggcc ggcatcatcg
     5641 gcgcgatcct ggcggcggcg gcaaccggct ttgttgggct ggcagtgtcg atccgcaacc
     5701 gagtgaagcg cgtgcgcgac ctgttggtga tggagccggg tgcagagcca taagggatcc
     5761 ccctcaagtc aaaagcctcc ggtcggaggc ttttgacttt ctgctatgga ggtcaggtat
     5821 gatttttatg acaacttgac ggctacatca ttcacttttt cttcacaacc ggcacggaac
     5881 tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag ttgatcgtca
     5941 aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa aagcagcttc
     6001 gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa ctgctggcgg
     6061 aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct ggcgatatca
     6121 aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac ccgattatcc
     6181 atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc
     6241 agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc
     6301 ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg
     6361 gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg aaagtaaacc
     6421 cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat ctctcctggc
     6481 gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt tttcaccacc
     6541 ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata
     6601 aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcatta
     6661 aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt catactcccg
     6721 ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt
     6781 tactggctct tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa
     6841 agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa
     6901 gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata
     6961 agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg
     7021 tttttttggg ctagcaccgc ctatctcgtg tgagataggc ggagatacga actttaagaa
     7081 ggagatatac ccatggtaaa atggtcagta ttgagcgata tctagagaat tcgtc
//
