LOCUS       V014600                10750 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014600
VERSION     V014600
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10750)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10750
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(702..719)
                     /label="6xHis"
                     /note="6xHis affinity tag"
     protein_bind    740..773
                     /label="attB"
                     /note="minimal attB site for the phi-C31 integrase (Groth
                     et al., 2000)"
     CDS             complement(923..1798)
                     /label="araC"
                     /note="L-arabinose regulatory protein"
     promoter        1825..2109
                     /label="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite
                     direction (Guzman et al., 1995)"
     CDS             2150..2854
                     /gene="SCEI"
                     /label="Intron-encoded endonuclease I-SceI"
                     /note="Intron-encoded endonuclease I-SceI from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P03882"
     CDS             3327..5141
                     /label="phage phi-C31 integrase"
                     /note="integrase from phage phi-C31"
     CDS             5612..7426
                     /label="phage phi-C31 integrase"
                     /note="integrase from phage phi-C31"
     CDS             complement(7619..8410)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     terminator      8943..9029
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      9121..9148
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     rep_origin      9282..9870
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(10056..10196)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
ORIGIN
        1 acattaccct gttatcccta gatgacatta ccctgttatc ccagatgaca ttaccctgtt
       61 atccctagat gacattaccc tgttatccct agatgacatt taccctgtta tccctagatg
      121 acattaccct gttatcccag atgacattac cctgttatcc ctagatacat taccctgtta
      181 tcccagatga cataccctgt tatccctaga tgacattacc ctgttatccc agatgacatt
      241 accctgttat ccctagatac attaccctgt tatcccagat gacataccct gttatcccta
      301 gatgacatta ccctgttatc ccagatgaca ttaccctgtt atccctagat acattaccct
      361 gttatcccag atgacatacc ctgttatccc tagatgacat taccctgtta tcccagatga
      421 cattaccctg ttatccctag atacattacc ctgttatccc agatgacata ccctgttatc
      481 cctagatgac attaccctgt tatcccagat gacattaccc tgttatccct agatacatta
      541 ccctgttatc ccagatgaca taccctgtta tccctagatg acattaccct gttatcccag
      601 atgacattac cctgttatcc ctagatacat taccctgtta tcccagatga cataccctgt
      661 tatccctaga tgacattacc ctgttatccc agataaactc aatgatgatg atgatgatgg
      721 tcgagactca gcggccgcgg tgccagggcg tgcccttggg ctccccgggc gcgactagtg
      781 aattcagatc tgatatctct agagtcgacc catgggggcc cgccccaact ggggtaacct
      841 ttgagttctc tcagttgggg gtaatcagca tcatgatgtg gtaccacatc atgatgctga
      901 ttataagaat gcggccgcct tatgacaact tgacggctac atcattcact ttttcttcac
      961 aaccggcacg gaactcgctc gggctggccc cggtgcattt tttaaatacc cgcgagaaat
     1021 agagttgatc gtcaaaacca acattgcgac cgacggtggc gataggcatc cgggtggtgc
     1081 tcaaaagcag cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag acgctaatcc
     1141 ctaactgctg gcggaaaaga tgtgacagac gcgacggcga caagcaaaca tgctgtgcga
     1201 cgctggcgat atcaaaattg ctgtctgcca ggtgatcgct gatgtactga caagcctcgc
     1261 gtacccgatt atccatcggt ggatggagcg actcgttaat cgcttccatg cgccgcagta
     1321 acaattgctc aagcagattt atcgccagca gctccgaata gcgcccttcc ccttgcccgg
     1381 cgttaatgat ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa
     1441 agaaccccgt attggcaaat attgacggcc agttaagcca ttcatgccag taggcgcgcg
     1501 gacgaaagta aacccactgg tgataccatt cgcgagcctc cggatgacga ccgtagtgat
     1561 gaatctctcc tggcgggaac agcaaaatat cacccggtcg gcaaacaaat tctcgtccct
     1621 gatttttcac caccccctga ccgcgaatgg tgagattgag aatataacct ttcattccca
     1681 gcggtcggtc gataaaaaaa tcgagataac cgttggcctc aatcggcgtt aaacccgcca
     1741 ccagatgggc attaaacgag tatcccggca gcaggggatc attttgcgct tcagccatac
     1801 ttttcatact cccgccattc agagaagaaa ccaattgtcc atattgcatc agacattgcc
     1861 gtcactgcgt cttttactgg ctcttctcgc taaccaaacc ggtaaccccg cttattaaaa
     1921 gcattctgta acaaagcggg accaaagcca tgacaaaaac gcgtaacaaa agtgtctata
     1981 atcacggcag aaaagtccac attgattatt tgcacggcgt cacactttgc tatgccatag
     2041 catttttatc cataagatta gcggatccta cctgacgctt tttatcgcaa ctctctactg
     2101 tttctccata cccgtttttt tgggctagca ggagggtacc tatatgcata tgaaaaacat
     2161 caaaaaaaac caggtaatga acctgggtcc gaactctaaa ctgctgaaag aatacaaatc
     2221 ccagctgatc gaactgaaca tcgaacagtt cgaagcaggt atcggtctga tcctgggtga
     2281 tgcttacatc cgttctcgtg atgaaggtaa aacctactgt atgcagttcg agtggaaaaa
     2341 caaagcatac atggaccacg tatgtctgct gtacgatcag tgggtactgt ccccgccgca
     2401 caaaaaagaa cgtgttaacc acctgggtaa cctggtaatc acctggggcg cccagacttt
     2461 caaacaccaa gctttcaaca aactggctaa cctgttcatc gttaacaaca aaaaaaccat
     2521 cccgaacaac ctggttgaaa actacctgac cccgatgtct ctggcatact ggttcatgga
     2581 tgatggtggt aaatgggatt acaacaaaaa ctctaccaac aaatcgatcg tactgaacac
     2641 ccagtctttc actttcgaag aagtagaata cctggttaag ggtctgcgta acaaattcca
     2701 actgaactgt tacgtaaaaa tcaacaaaaa caaaccgatc atctacatcg attctatgtc
     2761 ttacctgatc ttctacaacc tgatcaaacc gtacctgatc ccgcagatga tgtacaaact
     2821 gccgaacact atctcctccg aaactttcct gaaataatgc ctggcggcag tagcgcggtg
     2881 gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg
     2941 gggtctcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc
     3001 gaaagactgg gccttacttt tcatactccc gccattcaga gaagaaacca attgtccata
     3061 ttgcatcaga cattgccgtc actgcgtctt ttactggctc ttctcgctaa ccaaaccggt
     3121 aaccccgctt attaaaagca ttctgtaaca aagcgggacc aaagccatga caaaaacgcg
     3181 taacaaaagt gtctataatc acggcagaaa agtccacatt gattatttgc acggcgtcac
     3241 actttgctat gccatagcat ttttatccat aagattagcg gatcctacct gacgcttttt
     3301 atcgcaactc tctactgttt ctccatatgg acacgtacgc gggtgcttac gaccgtcagt
     3361 cgcgcgagcg cgaaaattcg agcgcagcaa gcccagcgac acagcgtagc gccaacgaag
     3421 acaaggcggc cgaccttcag cgcgaagtcg agcgcgacgg gggccggttc aggttcgtcg
     3481 ggcatttcag cgaagcgccg ggcacgtcgg cgttcgggac ggcggagcgc ccggagttcg
     3541 aacgcatcct gaacgaatgc cgcgccgggc ggctcaacat gatcattgtc tatgacgtgt
     3601 cgcgcttctc gcgcctgaag gtcatggacg cgattccgat tgtctcggaa ttgctcgccc
     3661 tgggcgtgac gattgtttcc actcaggaag gcgtcttccg gcagggaaac gtcatggacc
     3721 tgattcacct gattatgcgg ctcgacgcgt cgcacaaaga atcttcgctg aagtcggcga
     3781 agattctcga cacgaagaac cttcagcgcg aattgggcgg gtacgtcggc gggaaggcgc
     3841 cttacggctt cgagcttgtt tcggagacga aggagatcac gcgcaacggc cgaatggtca
     3901 atgtcgtcat caacaagctt gcgcactcga ccactcccct taccggaccc ttcgagttcg
     3961 agcccgacgt aatccggtgg tggtggcgtg agatcaagac gcacaaacac cttcccttca
     4021 agccgggcag tcaagccgcc attcacccgg gcagcatcac ggggctttgt aagcgcatgg
     4081 acgctgacgc cgtgccgacc cggggcgaga cgattgggaa gaagaccgct tcaagcgcct
     4141 gggacccggc aaccgttatg cgaatccttc gggacccgcg tattgcgggc ttcgccgctg
     4201 aggtgatcta caagaagaag ccggacggca cgccgaccac gaagattgag ggttaccgca
     4261 ttcagcgcga cccgatcacg ctccggccgg tcgagcttga ttgcggaccg atcatcgagc
     4321 ccgctgagtg gtatgagctt caggcgtggt tggacggcag ggggcgcggc aaggggcttt
     4381 cccgggggca agccattctg tccgccatgg acaagctgta ctgcgagtgt ggcgccgtca
     4441 tgacttcgaa gcgcggggaa gaatcgatca aggactctta ccgctgccgt cgccggaagg
     4501 tggtcgaccc gtccgcacct gggcagcacg aaggcacgtg caacgtcagc atggcggcac
     4561 tcgacaagtt cgttgcggaa cgcatcttca acaagatcag gcacgccgaa ggcgacgaag
     4621 agacgttggc gcttctgtgg gaagccgccc gacgcttcgg caagctcact gaggcgcctg
     4681 agaagagcgg cgaacgggcg aaccttgttg cggagcgcgc cgacgccctg aacgcccttg
     4741 aagagctgta cgaagaccgc gcggcaggcg cgtacgacgg acccgttggc aggaagcact
     4801 tccggaagca acaggcagcg ctgacgctcc ggcagcaagg ggcggaagag cggcttgccg
     4861 aacttgaagc cgccgaagcc ccgaagcttc cccttgacca atggttcccc gaagacgccg
     4921 acgctgaccc gaccggccct aagtcgtggt gggggcgcgc gtcagtagac gacaagcgcg
     4981 tgttcgtcgg gctcttcgta gacaagatcg ttgtcacgaa gtcgactacg ggcagggggc
     5041 agggaacgcc catcgagaag cgcgcttcga tcacgtgggc gaagccgccg accgacgacg
     5101 acgaagacga cgcccaggac ggcacggaag acgtagcggc gtagtgcctg gcggcagtag
     5161 cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg
     5221 tagtgtgggg tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg
     5281 ctcagtcgaa agactgggcc ttttttcata ctcccgccat tcagagaaga aaccaattgt
     5341 ccatattgca tcagacattg ccgtcactgc gtcttttact ggctcttctc gctaaccaaa
     5401 ccggtaaccc cgcttattaa aagcattctg taacaaagcg ggaccaaagc catgacaaaa
     5461 acgcgtaaca aaagtgtcta taatcacggc agaaaagtcc acattgatta tttgcacggc
     5521 gtcacacttt gctatgccat agcattttta tccataagat tagcggatcc tacctgacgc
     5581 tttttatcgc aactctctac tgtttctcca tatggacacc tatgctggtg cgtacgatcg
     5641 tcagtctcgt gaacgtgaaa actcttctgc agcgtctccg gctacccagc gctccgcgaa
     5701 cgaagataaa gcggcagacc tgcaacgtga ggtggagcgt gacggcggtc gcttccgttt
     5761 cgtgggccat tttagcgaag ccccgggtac tagcgcgttc ggcaccgcag aacgtcctga
     5821 attcgaacgc atcctgaatg aatgtcgcgc tggccgtctg aacatgatca tcgtttatga
     5881 tgtttctcgc ttctctcgtc tgaaagtcat ggacgcaatc ccgatcgttt ccgaactgct
     5941 ggcgctgggc gtgaccattg tttccactca ggagggcgtt ttccgtcagg gtaacgttat
     6001 ggacctgatc catctgatca tgcgtctgga tgctagccac aaagaatcct ccctgaagtc
     6061 tgctaaaatt ctggacacta agaatctgca gcgcgaactg ggcggttatg tgggtggcaa
     6121 agcgccgtac ggcttcgaac tggttagcga aaccaaagaa attacccgta atggtcgtat
     6181 ggttaacgtg gtaatcaaca agctggcaca ctccaccacg cctctgaccg gtccgttcga
     6241 attcgagccg gatgtaatcc gctggtggtg gcgtgaaatt aaaactcata aacacctgcc
     6301 gttcaaacca ggttctcagg ctgctattca tccgggctct attaccggtc tgtgcaaacg
     6361 catggacgcc gacgcagtac cgacccgtgg cgaaactatt ggcaagaaga ccgcgagctc
     6421 tgcttgggat ccggcaaccg ttatgcgtat cctgcgcgac ccgcgtattg cgggtttcgc
     6481 cgcagaggta atctacaaaa aaaagccaga cggcactccg acgactaaga ttgagggtta
     6541 ccgcatccag cgtgacccga tcaccctgcg cccggttgaa ctggactgtg gtccgatcat
     6601 cgaaccagct gaatggtacg aactgcaggc ttggctggac ggccgtggtc gcggcaaagg
     6661 cctgtcccgt ggccaagcta tcctgagcgc tatggataaa ctgtactgtg aatgcggcgc
     6721 ggtgatgacc agcaaacgtg gtgaagagag catcaaagat tcctaccgtt gccgtcgtcg
     6781 taaagttgtt gatccttctg cgcctggtca gcatgaaggt acctgtaacg tttccatggc
     6841 tgcgctggac aaattcgtag ctgaacgtat tttcaacaag attcgccacg cggaaggcga
     6901 tgaggagacc ctggcgctgc tgtgggaagc agcgcgccgt ttcggtaaac tgactgaagc
     6961 accggaaaaa tccggcgaac gcgcgaacct ggttgccgaa cgtgctgatg ctctgaacgc
     7021 actggaagaa ctgtacgaag atcgtgccgc gggcgcatat gatggtccgg ttggtcgtaa
     7081 acacttccgt aaacagcaag cagcactgac cctgcgtcag cagggcgctg aagaacgcct
     7141 ggcggagctg gaggccgcag aagcgccgaa actgccgctg gatcaatggt tcccagaaga
     7201 tgcggacgca gatccgaccg gtccgaaaag ctggtggggt cgtgcatctg ttgacgacaa
     7261 acgtgttttt gttggtctgt tcgtcgacaa aatcgttgtg accaaatcca ccacgggtcg
     7321 tggtcagggt accccgatcg aaaaacgcgc gtccatcact tgggctaaac cgccgactga
     7381 cgacgatgaa gacgatgctc aagacggcac cgaagacgtc gcggcttagt gcctggcggc
     7441 agtagcgcgg tggtcccacc tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc
     7501 gatggtagtg tggggtctcc ccatgcgaga gtagggaact gccaggcatc aaataaaacg
     7561 aaaggctcag tcgaaagact gggccttaca ctctagtgga tctcgagtta ataattcaga
     7621 agaactcgtc aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt
     7681 aaagcacgag gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag
     7741 ccaacgctat gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag
     7801 aaaagcggcc attttccacc atgatattcg gcaagcaggc atcgccatgg gtcacgacga
     7861 gatcctcgcc gtcgggcatg ctcgccttga gcctggcgaa cagttcggct ggcgcgagcc
     7921 cctgatgctc ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg
     7981 ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat
     8041 gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg
     8101 acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga
     8161 caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg
     8221 cctcgtcttg cagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc
     8281 gcccctgcgc tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc
     8341 agtcatagcc gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt
     8401 gttcaatcat gcgaaacgat cctcatcctg tctcttgatc agagcttgat cccctgcgcc
     8461 atcagatcct tggcggcgag aaagccatcc agtttacttt gcagggcttc ccaaccttac
     8521 cagagggcgc cccagctggc aattccggtt cgcttgctgt ccataaaacc gcccagtcta
     8581 gctatcgcca tgtaagccca ctgcaagcta cctgctttct ctttgcgctt gcgttttccc
     8641 ttgtccagat agcccagtag ctgacattca tccggggtca gcaccgtttc tgcggactgg
     8701 ctttctacgt gctcgagggg ggccaaacgg tctccagctt ggctgttttg gcggatgaga
     8761 gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagaa
     8821 tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa
     8881 acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc
     8941 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     9001 cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc
     9061 aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaattaagc
     9121 agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg tttatttttc
     9181 taaatacatt caaatatgta tccgctcatg accaaaatcc cttaacgtga gttttcgttc
     9241 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     9301 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     9361 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     9421 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     9481 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     9541 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     9601 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     9661 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     9721 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     9781 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     9841 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     9901 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     9961 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
    10021 cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg
    10081 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
    10141 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
    10201 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
    10261 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
    10321 cgaaacgcgc gaggcagcag atcaattcgc gcgcgaaggc gaagcggcat gcataatgtg
    10381 cctgtcaaat ggacgaagca gggattctgc aaaccctatg ctactccgtc aagccgtcaa
    10441 ttgtctgatt cgttaccaat tatgacaact tgacggctac atcattcact ttttcttcac
    10501 aaccggcacg gaactcgctc gggctggccc cggtgcattt tttaaatacc cgcgagaaat
    10561 agagttgatc gtcaaaacca acattgcgac cgacggtggc gataggcatc cgggtggtgc
    10621 tcaaaagcag cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag acgctaatcc
    10681 ctaactgctg gcggaaaaga tgtgacagac gcgacggcga caagcaaaca tgctgtgcga
    10741 cgctggcgat
//
