LOCUS       40924_6691        4740 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Reporter vector pBLmIL6CAT, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 4740)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4740)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4740
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      42..519
                     /label=mIL6
                     /note="mIL6"
                     /regulatory_class="promoter"
     CDS             578..1234
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             1327..1401
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL88386.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     intron          1403..1468
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1598..1618
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2043..2177
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2213..2229)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2237..2253)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2261..2291)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2306..2327)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2615..3203)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3377..4234)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4235..4339)
                     /label=AmpR promoter
     polyA_signal    4366..4500
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(4366..4440)
                     /label=polyA
                     /note="polyA"
                     /regulatory_class="terminator"
     regulatory      4366..4440
                     /label=polyA
                     /note="polyA"
                     /regulatory_class="terminator"
     regulatory      complement(4581..4677)
                     /label=polyA
                     /note="polyA"
                     /regulatory_class="terminator"
     regulatory      4581..4677
                     /label=polyA
                     /note="polyA"
                     /regulatory_class="terminator"
     polyA_signal    4603..4737
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccttac agacatacaa aagaatccta
       61 gcctcttatt catgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgta
      121 tgtgtgtgtc gtctgtcatg cgcgcgtgcc tgcgtttaaa taacatcagc tttacgttct
      181 ctttctcctt ataaaacatt gtgaatttca gttttctttc ccatcaagac atgctcaagt
      241 gctgagtcac ttttaaagaa aaaaagaaga gtgctcatgc ttcttagggc tagcctcaag
      301 gatgacttaa gcacactttt cccttcctag ttgtgattct ttcgatgcta aacgacgtca
      361 cattgtgcaa tcttaataag gtttccaatc agccccaccc actctggccc cacccccacc
      421 ctccaacaaa gatttttatc aaatgtggga ttttcccatg agtctcaaaa ttagagagtt
      481 gactcctaat aaatatgaga ctggggatgt ctgtagctca ttctgctctg ggatctcgag
      541 gagcttggcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata
      601 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
      661 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt
      721 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa
      781 tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt
      841 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga
      901 ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg
      961 tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa
     1021 tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc
     1081 ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc
     1141 gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt
     1201 acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca gttattggtg
     1261 cccttaaacg cctggtgcta cgcctgaata agtgataata agcggatgaa tggcagaaat
     1321 tcgccggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc
     1381 tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact
     1441 actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca
     1501 gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga
     1561 tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga
     1621 agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa
     1681 tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa
     1741 gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa
     1801 catactgttt tttcttactc cacacaggca tagagtgtct gctattaata actatgctca
     1861 aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta
     1921 tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     1981 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     2041 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     2101 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     2161 cttatcatgt ctggatcgat ccccgggtac cgagctcgaa ttcgtaatca tggtcatagc
     2221 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     2281 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     2341 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     2401 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     2461 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     2521 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     2581 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     2641 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     2701 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     2761 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     2821 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     2881 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     2941 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3001 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     3061 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3121 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3181 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     3241 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     3301 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     3361 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     3421 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     3481 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     3541 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     3601 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     3661 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     3721 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     3781 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     3841 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     3901 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     3961 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     4021 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     4081 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     4141 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     4201 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     4261 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     4321 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgaactt gtttattgca
     4381 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     4441 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     4501 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     4561 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     4621 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     4681 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctcg
//
