LOCUS       40924_6686        5422 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Reporter vector pBLHIL6CAT, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5422)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5422)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5422)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5422)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5422
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    32..672
                     /label=5FR
                     /note="5FR"
     regulatory      673..1208
                     /label=hIL6
                     /note="hIL6"
                     /regulatory_class="promoter"
     CDS             1260..1916
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             2009..2083
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL88813.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     intron          2085..2150
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2280..2300
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2725..2859
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2895..2911)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2919..2935)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2943..2973)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2988..3009)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3297..3885)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4059..4916)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4917..5021)
                     /label=AmpR promoter
     polyA_signal    5048..5182
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(5048..5122)
                     /label=polyA
                     /note="polyA"
                     /regulatory_class="terminator"
     regulatory      5048..5122
                     /label=polyA
                     /note="polyA"
                     /regulatory_class="terminator"
     regulatory      complement(5263..5359)
                     /label=polyA
                     /note="polyA"
                     /regulatory_class="terminator"
     regulatory      5263..5359
                     /label=polyA
                     /note="polyA"
                     /regulatory_class="terminator"
     polyA_signal    5285..5419
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatcctcct gcaagagaca ccatcctgag
       61 ggaagagggc ttctgaacca gcttgaccca ataagaaatt cttgggtgcc gacgcggaag
      121 cagattcaga gcctagagcc gtgcctgcgt ccgtagtttc cttctagctt cttttgattt
      181 caaatcaaga cttacaggga gagggagcga taaacacaaa ctctgcaaga tgccacaagg
      241 tcctcctttg acatccccaa caaagaggtg agtagtattc tccccctttc tgccctgaac
      301 caagtgggct tcagtaattt cagggctcca ggagacctgg ggcccatgca ggtgccccag
      361 tgaaacagtg gtgaagagac tcagtggcaa tggggagagc actggcagca caaggcaaac
      421 ctctggcaca gagagcaaag tcctcactgg gaggattccc aaggggtcac ttgggagagg
      481 gcagggcagc agccaacctc ctctaagtgg gctgaagcag gtgaagaaag tggcagaagc
      541 cacgcggtgg caaaaaggag tcacacactc cacctggaga cgccttgaag taactgcacg
      601 aaatttgagg atggccaggc agttctacaa cagccgctca cagggagagc cagaacacag
      661 aagaactcag atgactggta gtattacctt cttcataatc ccaggcttgg ggggctgcga
      721 tggagtcaga ggaaactcag ttcagaacat ctttggtttt tacaaataca aattaactgg
      781 aacgctaaat tctagcctgt taatctggtc actgaaaaaa aatttttttt ttttcaaaaa
      841 acatagcttt agcttatttt ttttctcttt gtaaaacttc gtgcatgact tcagctttac
      901 tctttgtcaa gacatgccaa agtgctgagt cactaataaa agaaaaaaag aaagtaaagg
      961 aagagtggtt ctgcttctta gcgctagcct caatgacgac ctaagctgca cttttccccc
     1021 tagttgtgtc ttgcgatgct aaaggacgtc acattgcaca atcttaataa ggtttccaat
     1081 cagccccacc cgctctggcc ccaccctcac cctccaacaa agatttatca aatgtgggat
     1141 tttcccatga gtctcaatat tagagtctca acccccaata aatataggac tggagatgtc
     1201 tgaggctcat tctgccctcg aggagcttgg cgagattttc aggagctaag gaagctaaaa
     1261 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
     1321 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
     1381 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
     1441 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg
     1501 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
     1561 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
     1621 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
     1681 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
     1741 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
     1801 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
     1861 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat
     1921 ttttttaagg cagttattgg tgcccttaaa cgcctggtgc tacgcctgaa taagtgataa
     1981 taagcggatg aatggcagaa attcgccgga tctttgtgaa ggaaccttac ttctgtggtg
     2041 tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt
     2101 taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct
     2161 atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct
     2221 cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc
     2281 caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt
     2341 tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg
     2401 aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta
     2461 ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt
     2521 ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg
     2581 ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca
     2641 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     2701 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     2761 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     2821 ttgtccaaac tcatcaatgt atcttatcat gtctggatcg atccccgggt accgagctcg
     2881 aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     2941 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     3001 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     3061 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     3121 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     3181 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     3241 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     3301 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     3361 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     3421 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     3481 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     3541 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     3601 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     3661 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     3721 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     3781 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     3841 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     3901 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     3961 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     4021 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     4081 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     4141 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     4201 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     4261 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     4321 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     4381 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     4441 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     4501 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     4561 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     4621 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     4681 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     4741 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     4801 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     4861 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     4921 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     4981 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     5041 ccacctgaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     5101 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     5161 tgtatcttat catgtctgga tcataatcag ccataccaca tttgtagagg ttttacttgc
     5221 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     5281 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     5341 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     5401 atcttatcat gtctggatct cg
//
