LOCUS       40924_6666        4252 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Reporter vector pBLCAT6, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 4252)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4252)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             90..746
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             839..913
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL88717.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     intron          915..980
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1110..1130
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    1555..1689
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1725..1741)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1749..1765)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1773..1803)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1818..1839)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2127..2715)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2889..3746)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3747..3851)
                     /label=AmpR promoter
     polyA_signal    3878..4012
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(3878..3952)
                     /label=SV40 polyA late
                     /note="SV40 polyA late"
                     /regulatory_class="terminator"
     regulatory      3878..3952
                     /label=SV40 polyA early
                     /note="SV40 polyA early"
                     /regulatory_class="terminator"
     regulatory      complement(4093..4189)
                     /label=SV40 polyA late
                     /note="SV40 polyA late"
                     /regulatory_class="terminator"
     regulatory      4093..4189
                     /label=SV40 polyA early
                     /note="SV40 polyA early"
                     /regulatory_class="terminator"
     polyA_signal    4115..4249
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccagat ctggatctcg aggagcttgg
       61 cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg
      121 ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat
      181 gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa
      241 ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc
      301 cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt
      361 gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg
      421 acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc
      481 tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg
      541 tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt
      601 tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg
      661 ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt
      721 actgcgatga gtggcagggc ggggcgtaat ttttttaagg cagttattgg tgcccttaaa
      781 cgcctggtgc tacgcctgaa taagtgataa taagcggatg aatggcagaa attcgccgga
      841 tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga
      901 tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc
      961 taattgtttg tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga
     1021 atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg
     1081 ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca
     1141 aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc
     1201 ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta
     1261 tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt
     1321 tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt
     1381 gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct
     1441 tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     1501 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     1561 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     1621 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     1681 gtctggatcg atccccgggt accgagctcg aattcgtaat catggtcata gctgtttcct
     1741 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     1801 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     1861 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     1921 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     1981 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     2041 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     2101 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     2161 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     2221 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     2281 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     2341 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     2401 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     2461 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     2521 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     2581 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     2641 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     2701 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     2761 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     2821 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     2881 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     2941 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     3001 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     3061 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     3121 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     3181 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     3241 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     3301 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     3361 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     3421 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     3481 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     3541 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     3601 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     3661 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     3721 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     3781 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     3841 ggggttccgc gcacatttcc ccgaaaagtg ccacctgaac ttgtttattg cagcttataa
     3901 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     3961 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcataatcag
     4021 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     4081 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     4141 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     4201 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct cg
//
