LOCUS       40924_6661        4404 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Reporter vector pBLCAT5, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 4404)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4404)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        112..174
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     CDS             242..898
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             991..1065
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL88714.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     intron          1067..1132
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1262..1282
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    1707..1841
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1877..1893)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1901..1917)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1925..1955)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1970..1991)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2279..2867)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3041..3898)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3899..4003)
                     /label=AmpR promoter
     polyA_signal    4030..4164
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(4030..4104)
                     /label=SV40 polyA late
                     /note="SV40 polyA late"
                     /regulatory_class="terminator"
     regulatory      4030..4104
                     /label=SV40 polyA early
                     /note="SV40 polyA early"
                     /regulatory_class="terminator"
     regulatory      complement(4245..4341)
                     /label=SV40 polyA late
                     /note="SV40 polyA late"
                     /regulatory_class="terminator"
     regulatory      4245..4341
                     /label=SV40 polyA early
                     /note="SV40 polyA early"
                     /regulatory_class="terminator"
     polyA_signal    4267..4401
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccggcc ccgcccagcg tcttgtcatt
       61 ggcgaattcg aacacgcaga tgcagtcggg gcggcgcggt ccgaggtcca cttcgcatat
      121 taaggtgacg cgtgtggcct cgaacaccga gcgaccctgc agcgacccgc ttaacagcgt
      181 caacagcgtg ccgcagatct cgaggagctt ggcgagattt tcaggagcta aggaagctaa
      241 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
      301 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
      361 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
      421 tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg
      481 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
      541 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
      601 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
      661 gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
      721 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg
      781 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca
      841 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta
      901 atttttttaa ggcagttatt ggtgccctta aacgcctggt gctacgcctg aataagtgat
      961 aataagcgga tgaatggcag aaattcgccg gatctttgtg aaggaacctt acttctgtgg
     1021 tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt
     1081 tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac
     1141 ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg
     1201 ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc
     1261 tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt
     1321 tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa
     1381 ggaaaaagct gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag
     1441 taggcataac agttataatc ataacatact gttttttctt actccacaca ggcatagagt
     1501 gtctgctatt aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg
     1561 ggttaataag gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca
     1621 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     1681 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     1741 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     1801 gtttgtccaa actcatcaat gtatcttatc atgtctggat cgatccccgg gtaccgagct
     1861 cgaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     1921 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     1981 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     2041 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     2101 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     2161 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     2221 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     2281 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     2341 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     2401 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2461 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     2521 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     2581 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     2641 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     2701 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     2761 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     2821 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     2881 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     2941 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     3001 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     3061 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     3121 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     3181 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     3241 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     3301 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     3361 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     3421 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     3481 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     3541 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     3601 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     3661 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     3721 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     3781 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     3841 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     3901 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     3961 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     4021 tgccacctga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     4081 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     4141 aatgtatctt atcatgtctg gatcataatc agccatacca catttgtaga ggttttactt
     4201 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     4261 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     4321 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     4381 gtatcttatc atgtctggat ctcg
//
