LOCUS       ampR_gene;_beta-        4901 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBLCAT3T-p4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    ampR gene; beta-lactamase; cat gene; chloramphenicol acetyltra.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Luckow B, Schutz G.
  TITLE     CAT constructions with multiple unique restriction sites for the 
            functional analysis of eukaryotic promoters and regulatory elements
  JOURNAL   Nucleic Acids Res. 15 (13), 5490 (1987)
  PUBMED    3037497
REFERENCE   2  (bases 1 to 4901)
  AUTHORS   Lobo SM, Hernandez N.
  TITLE     A 7 bp mutation converts a human RNA polymerase II snRNA promoter 
            into an RNA polymerase III promoter
  JOURNAL   Cell 58 (1), 55-67 (1989)
  PUBMED    2752422
REFERENCE   3  (bases 1 to 4901)
  AUTHORS   Principaud E, Spohr G.
  TITLE     Xenopus laevis c-myc I and II genes: molecular structure and 
            developmental expression
  JOURNAL   Nucleic Acids Res. 19 (11), 3081-3088 (1991)
  PUBMED    2057364
REFERENCE   4  (bases 1 to 4901)
  AUTHORS   Bernhardt K.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2007) Bernhardt K., The Gurdon Institute, 
            University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, 
            UNITED KINGDOM
REFERENCE   5  (bases 1 to 4901)
  TITLE     Direct Submission
REFERENCE   6  (bases 1 to 4901)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1987"; volume: "15"; issue: "13"; pages: "5490"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Cell"; 
            date: "1989"; volume: "58"; issue: "1"; pages: "55-67"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1991"; volume: "19"; issue: "11"; pages: 
            "3081-3088"
COMMENT     SGRef: number: 4; type: "Journal Article"; journalName: "Submitted 
            (16-APR-2007) Bernhardt K., The Gurdon Institute, University of 
            Cambridge, Tennis Court Road, Cambridge CB2 1QN, UNITED KINGDOM"
COMMENT     SGRef: number: 5; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          796..1001
                     /chromosome="6"
                     /map="6F2"
                     /mol_type="other DNA"
                     /db_xref="taxon:10090"
                     /organism="Mus musculus"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      440..453
                     /note="this is a nonfunctional RNA polymerase II terminator
                     (3' box), termination depends on the presence of an 
                     appropriate snRNA promoter upstream such as in the human U1
                     and U2 snRNA genes"
                     /citation=[2]
                     /citation=[3]
                     /regulatory_class="terminator"
     regulatory      474..481
                     /note="this is a functional RNA polymerase III terminator
                     from the human U6 snRNA gene"
                     /citation=[2]
                     /citation=[3]
                     /regulatory_class="terminator"
     misc_feature    755..795
                     /note="insert of unknown origin, putative bacterial 16S
                     rRNA gene fragment"
     regulatory      796..1001
                     /gene="Nanog"
                     /note="206 bp of the mouse Nanog promoter -207 to -2 with 
                     respect to the transcriptional start site (-397 to -192 
                     with respect to the Nanog start codon)"
                     /regulatory_class="promoter"
     gene            796..1001
                     /gene="Nanog"
                     /label=Nanog
     CDS             1045..1701
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     intron          1870..1935
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2065..2085
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2510..2644
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2680..2696)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2704..2720)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2728..2758)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2773..2794)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3082..3670)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3844..4701)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4702..4806)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcagctcg
      421 acccccctga ctttctggag tttcaaaaac agaccgtacg ccaagggtca tgtctttttt
      481 cgtattggtt tgtgtcttag ttgttaatcc tacagtggag ggatcgatcc tctagcttcc
      541 tggagcgcca ctcgccctac ttccgcagcc acagtgcgca gattaggagc gccacttctt
      601 tttgtcactt gaaaaacatg taaaaataat gtactaggag acactttcaa taaaggcaaa
      661 tgtttttatt tgtacactct cgggtgatta tttacccccc acccttgccg tctgcgccgt
      721 ttctagcgac ctgcaggtcg actctagagg atccgaaggg cgaattccag cacactggcg
      781 gccgttacta gtggatccca gtctgggtca ccttacagct tcttttgcat tacaatgtcc
      841 atggtggacc ctgcaggtgg gattaactgt gaattcacag ggctggtggg gcgtgggtgc
      901 cgcctgggtg cctgggagaa tagggggtgg gtagggtagg aggcttgagg ggggaggagc
      961 aggacctacc ctttaaatct atcgccttga gccgttggcc tctcgaggag cttggcgaga
     1021 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat
     1081 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc
     1141 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag
     1201 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa
     1261 ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac
     1321 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat
     1381 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc
     1441 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt
     1501 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc
     1561 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat
     1621 catgccgtct gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc
     1681 gatgagtggc agggcggggc gtaatttttt taaggcagtt attggtgccc ttaaacgcct
     1741 ggtgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg ccggatcttt
     1801 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
     1861 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
     1921 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc
     1981 tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact
     2041 gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac
     2101 tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct
     2161 tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa
     2221 aaatattctg taacctttat aagtaggcat aacagttata atcataacat actgtttttt
     2281 cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc
     2341 tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact
     2401 agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     2461 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     2521 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     2581 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     2641 gatcgatccc cgggtaccga gctcgaattc gtaatcatgg tcatagctgt ttcctgtgtg
     2701 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     2761 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     2821 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     2881 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     2941 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     3001 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     3061 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     3121 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     3181 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     3241 cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag
     3301 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     3361 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     3421 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     3481 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     3541 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     3601 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     3661 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     3721 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     3781 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     3841 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     3901 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     3961 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     4021 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     4081 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     4141 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     4201 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     4261 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     4321 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     4381 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     4441 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     4501 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     4561 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     4621 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     4681 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     4741 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     4801 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     4861 attaacctat aaaaataggc gtatcacgag gccctttcgt c
//
