LOCUS       ampR_gene;_beta-        4964 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBLCAT3T-NP192, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    ampR gene; beta-lactamase; cat gene; chloramphenicol acetyltra.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Luckow B, Schutz G.
  TITLE     CAT constructions with multiple unique restriction sites for the 
            functional analysis of eukaryotic promoters and regulatory elements
  JOURNAL   Nucleic Acids Res. 15 (13), 5490 (1987)
  PUBMED    3037497
REFERENCE   2  (bases 1 to 4964)
  AUTHORS   Lobo SM, Hernandez N.
  TITLE     A 7 bp mutation converts a human RNA polymerase II snRNA promoter 
            into an RNA polymerase III promoter
  JOURNAL   Cell 58 (1), 55-67 (1989)
  PUBMED    2752422
REFERENCE   3  (bases 1 to 4964)
  AUTHORS   Principaud E, Spohr G.
  TITLE     Xenopus laevis c-myc I and II genes: molecular structure and 
            developmental expression
  JOURNAL   Nucleic Acids Res. 19 (11), 3081-3088 (1991)
  PUBMED    2057364
REFERENCE   4  (bases 1 to 4964)
  AUTHORS   Bernhardt K.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2007) Bernhardt K., The Gurdon Institute, 
            University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, 
            UNITED KINGDOM
REFERENCE   5  (bases 1 to 4964)
  TITLE     Direct Submission
REFERENCE   6  (bases 1 to 4964)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1987"; volume: "15"; issue: "13"; pages: "5490"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Cell"; 
            date: "1989"; volume: "58"; issue: "1"; pages: "55-67"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1991"; volume: "19"; issue: "11"; pages: 
            "3081-3088"
COMMENT     SGRef: number: 4; type: "Journal Article"; journalName: "Submitted 
            (16-APR-2007) Bernhardt K., The Gurdon Institute, University of 
            Cambridge, Tennis Court Road, Cambridge CB2 1QN, UNITED KINGDOM"
COMMENT     SGRef: number: 5; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4964
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          755..1064
                     /chromosome="6"
                     /map="6F2"
                     /mol_type="other DNA"
                     /db_xref="taxon:10090"
                     /organism="Mus musculus"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      440..453
                     /note="this is a nonfunctional RNA polymerase II terminator
                     (3' box), termination depends on the presence of an 
                     appropriate snRNA promoter upstream such as in the human U1
                     and U2 snRNA genes"
                     /citation=[2]
                     /citation=[3]
                     /regulatory_class="terminator"
     regulatory      474..481
                     /note="this is a functional RNA polymerase III terminator
                     from the human U6 snRNA gene"
                     /citation=[2]
                     /citation=[3]
                     /regulatory_class="terminator"
     regulatory      755..1064
                     /gene="Nanog"
                     /note="120 bp of the mouse Nanog promoter and the 190 bp 
                     untranslated region, -120 to +190 with respect to the 
                     transcriptional start site (-310 to -1 with respect to the 
                     Nanog start codon)"
                     /regulatory_class="promoter"
     gene            755..1064
                     /gene="Nanog"
                     /label=Nanog
     CDS             1108..1764
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     intron          1933..1998
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2128..2148
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2573..2707
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2743..2759)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2767..2783)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2791..2821)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2836..2857)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3145..3733)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3907..4764)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4765..4869)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcagctcg
      421 acccccctga ctttctggag tttcaaaaac agaccgtacg ccaagggtca tgtctttttt
      481 cgtattggtt tgtgtcttag ttgttaatcc tacagtggag ggatcgatcc tctagcttcc
      541 tggagcgcca ctcgccctac ttccgcagcc acagtgcgca gattaggagc gccacttctt
      601 tttgtcactt gaaaaacatg taaaaataat gtactaggag acactttcaa taaaggcaaa
      661 tgtttttatt tgtacactct cgggtgatta tttacccccc acccttgccg tctgcgccgt
      721 ttctagcgac ctgcaggtcg actctagagg atccctggtg gggcgtgggt gccgcctggg
      781 tgcctgggag aatagggggt gggtagggta ggaggcttga ggggggagga gcaggaccta
      841 ccctttaaat ctatcgcctt gagccgttgg ccttcagata ggctgatttg gttggtgtct
      901 tgctctttct gtgggaaggc tgcggctcac ttccttctga cttcttgata attttgcatt
      961 agacatttaa ctcttctttc tatgatcttt ccttctagac actgagtttt ttggttgttg
     1021 cctaaaacct tttcagaaat cccttccctc gccatcacac tgacctcgag gagcttggcg
     1081 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
     1141 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
     1201 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
     1261 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
     1321 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
     1381 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
     1441 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
     1501 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
     1561 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     1621 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     1681 catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     1741 tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca gttattggtg cccttaaacg
     1801 cctggtgcta cgcctgaata agtgataata agcggatgaa tggcagaaat tcgccggatc
     1861 tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt
     1921 taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta
     1981 attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat
     2041 gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct
     2101 actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag
     2161 gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt
     2221 gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg
     2281 gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt
     2341 tttcttactc cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt
     2401 acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg
     2461 actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     2521 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     2581 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     2641 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     2701 ctggatcgat ccccgggtac cgagctcgaa ttcgtaatca tggtcatagc tgtttcctgt
     2761 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     2821 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     2881 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     2941 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     3001 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     3061 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     3121 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     3181 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     3241 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     3301 gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct
     3361 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     3421 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     3481 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     3541 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     3601 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     3661 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     3721 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     3781 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     3841 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     3901 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     3961 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     4021 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     4081 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     4141 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     4201 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     4261 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     4321 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     4381 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     4441 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     4501 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     4561 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     4621 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     4681 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     4741 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     4801 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     4861 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     4921 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc
//
