LOCUS       ampR_gene;_beta-        5004 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBLCAT3T-NP191, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    ampR gene; beta-lactamase; cat gene; chloramphenicol acetyltra.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Luckow B, Schutz G.
  TITLE     CAT constructions with multiple unique restriction sites for the 
            functional analysis of eukaryotic promoters and regulatory elements
  JOURNAL   Nucleic Acids Res. 15 (13), 5490 (1987)
  PUBMED    3037497
REFERENCE   2  (bases 1 to 5004)
  AUTHORS   Lobo SM, Hernandez N.
  TITLE     A 7 bp mutation converts a human RNA polymerase II snRNA promoter 
            into an RNA polymerase III promoter
  JOURNAL   Cell 58 (1), 55-67 (1989)
  PUBMED    2752422
REFERENCE   3  (bases 1 to 5004)
  AUTHORS   Principaud E, Spohr G.
  TITLE     Xenopus laevis c-myc I and II genes: molecular structure and 
            developmental expression
  JOURNAL   Nucleic Acids Res. 19 (11), 3081-3088 (1991)
  PUBMED    2057364
REFERENCE   4  (bases 1 to 5004)
  AUTHORS   Bernhardt K.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2007) Bernhardt K., The Gurdon Institute, 
            University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, 
            UNITED KINGDOM
REFERENCE   5  (bases 1 to 5004)
  TITLE     Direct Submission
REFERENCE   6  (bases 1 to 5004)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1987"; volume: "15"; issue: "13"; pages: "5490"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Cell"; 
            date: "1989"; volume: "58"; issue: "1"; pages: "55-67"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1991"; volume: "19"; issue: "11"; pages: 
            "3081-3088"
COMMENT     SGRef: number: 4; type: "Journal Article"; journalName: "Submitted 
            (16-APR-2007) Bernhardt K., The Gurdon Institute, University of 
            Cambridge, Tennis Court Road, Cambridge CB2 1QN, UNITED KINGDOM"
COMMENT     SGRef: number: 5; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5004
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          755..1104
                     /chromosome="6"
                     /map="6F2"
                     /mol_type="other DNA"
                     /db_xref="taxon:10090"
                     /organism="Mus musculus"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      440..453
                     /note="this is a nonfunctional RNA polymerase II terminator
                     (3' box), termination depends on the presence of an 
                     appropriate snRNA promoter upstream such as in the human U1
                     and U2 snRNA genes"
                     /citation=[2]
                     /citation=[3]
                     /regulatory_class="terminator"
     regulatory      474..481
                     /note="this is a functional RNA polymerase III terminator
                     from the human U6 snRNA gene"
                     /citation=[2]
                     /citation=[3]
                     /regulatory_class="terminator"
     regulatory      755..1104
                     /gene="Nanog"
                     /note="160 bp of the mouse Nanog promoter and the 190 bp 
                     untranslated region, -160 to +190 with respect to the 
                     transcriptional start site (-350 to -1 with respect to the 
                     Nanog start codon)"
                     /regulatory_class="promoter"
     gene            755..1104
                     /gene="Nanog"
                     /label=Nanog
     CDS             1148..1804
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     intron          1973..2038
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2168..2188
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2613..2747
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2783..2799)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2807..2823)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2831..2861)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2876..2897)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3185..3773)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3947..4804)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4805..4909)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcagctcg
      421 acccccctga ctttctggag tttcaaaaac agaccgtacg ccaagggtca tgtctttttt
      481 cgtattggtt tgtgtcttag ttgttaatcc tacagtggag ggatcgatcc tctagcttcc
      541 tggagcgcca ctcgccctac ttccgcagcc acagtgcgca gattaggagc gccacttctt
      601 tttgtcactt gaaaaacatg taaaaataat gtactaggag acactttcaa taaaggcaaa
      661 tgtttttatt tgtacactct cgggtgatta tttacccccc acccttgccg tctgcgccgt
      721 ttctagcgac ctgcaggtcg actctagagg atccggtgga ccctgcaggt gggattaact
      781 gtgaattcac agggctggtg gggcgtgggt gccgcctggg tgcctgggag aatagggggt
      841 gggtagggta ggaggcttga ggggggagga gcaggaccta ccctttaaat ctatcgcctt
      901 gagccgttgg ccttcagata ggctgatttg gttggtgtct tgctctttct gtgggaaggc
      961 tgcggctcac ttccttctga cttcttgata attttgcatt agacatttaa ctcttctttc
     1021 tatgatcttt ccttctagac actgagtttt ttggttgttg cctaaaacct tttcagaaat
     1081 cccttccctc gccatcacac tgacctcgag gagcttggcg agattttcag gagctaagga
     1141 agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg
     1201 taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca
     1261 gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc
     1321 ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa
     1381 agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca
     1441 aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca
     1501 catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt
     1561 tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt
     1621 aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac
     1681 gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg tctgtgatgg
     1741 cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg
     1801 ggcgtaattt ttttaaggca gttattggtg cccttaaacg cctggtgcta cgcctgaata
     1861 agtgataata agcggatgaa tggcagaaat tcgccggatc tttgtgaagg aaccttactt
     1921 ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat
     1981 aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat
     2041 tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct
     2101 gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc
     2161 tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct
     2221 aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac
     2281 cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt
     2341 tataagtagg cataacagtt ataatcataa catactgttt tttcttactc cacacaggca
     2401 tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg
     2461 taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc
     2521 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     2581 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     2641 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     2701 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcgat ccccgggtac
     2761 cgagctcgaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     2821 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     2881 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     2941 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     3001 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     3061 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     3121 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     3181 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     3241 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     3301 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     3361 gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc
     3421 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     3481 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     3541 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     3601 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     3661 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     3721 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     3781 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     3841 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     3901 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     3961 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     4021 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     4081 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     4141 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     4201 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     4261 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     4321 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     4381 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     4441 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     4501 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     4561 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     4621 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     4681 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     4741 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     4801 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     4861 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     4921 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     4981 ggcgtatcac gaggcccttt cgtc
//
