LOCUS       40924_6621        5064 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5064)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5064
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1554..1799)
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     CDS             complement(1878..1895)
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             complement(1911..1940)
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     CDS             complement(2012..2278)
                     /codon_start=1
                     /label=alpha-factor secretion signal
                     /note="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKREAEA"
     rep_origin      complement(3428..4016)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4190..5047)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 agctccaagg caacaaattg actactcaga ccgacattca ttcgttattg attttaaatc
       61 aacgataaac ggaatggtta cttgaatgat ttcactttat gatcattgtt tactaattac
      121 ctaaatagga ttttatatgg aattggaaga ataagggaaa tttcagatgt ctgaaaaagg
      181 cgaggagggt actaatcatt caagcccatt tcttgccagt aattgcttca taagcttcaa
      241 tatacttttc tttactcttg atagcaattt ctgcatccat ggctacgccc tctttgccat
      301 tcaatccgtt ggccgtcaac caatctctga gaaactgctt atcgtaactc tcttgcgatt
      361 tacccacttg gtaagtcttt tgattccaaa atctagaaga atctggagtt aaaacttcat
      421 ctactagtac caattcattg ttttcgtcca gtccaaattc gaatttcgta tcagcaataa
      481 tgatcccctt caaaagggcg aagttttttg cagcagaata caactcgacc gccttgacag
      541 cgaccttctc acaaatgtct ttacctacaa tctcagcagc ttgttcaata gagatgtttt
      601 catcgtgttc accctgttca gctttcgttg aaggtgtgaa aatcggagtt ggaaaggcgt
      661 cgctctcttg aaggttctcg ttttcaacct tgactccatg gacagttttt gagttcttgt
      721 actctttcca tgcacttcca gtgatgtaac ctctgacaat ggcttccaaa ggtatcagtc
      781 tgtgcttttt tactatcaag gatcgtccct ctaattgaga tttgtatttt tcttcagaca
      841 gttttgatgg tagtaaagca aagacttcct tgtcattaga agcaaccaaa tgattcttta
      901 tgtagggtgc caaaaaatca aaccagaaaa ctgagagctg agtcaaaatc tttcccttat
      961 caggaatacc gtttgtcata atcacatcgt aagcggagat acggtcagtt gcgacgaaca
     1021 gcaagttgtt ctcatcgact gcataaatgt ctctaacctt tcctttggcg attaaaggta
     1081 ggattccgtc cagatcagtg ttcacaatgg acatacttgg aaggatacag caaagtgtgt
     1141 tggaagcgat gacacatgga aaggaatttt tcgagtttcc tagagtagta tattggggcg
     1201 gtgaaagttc agatgtttaa tgcttaatac tcttatactc ttcaaagcgc ccaagtgttt
     1261 ctgccaacct gacttttttc tgaataatga atcgttcaag tggagtattt aaaccatgat
     1321 taagttacgt gatttggcac tggataaggt cgaaaaatat ccgtattcat aaacgattat
     1381 tggtaaaagt tacaaaatac cactaattac ggagaagctt agtaacagtt atcatctctt
     1441 ggtcgattaa cgcttacaat ttccattcgc cattcaggct gcgcaactgt tgggaagggc
     1501 gatcggtgcg ggcctcttcg ctattacgcc aggcggatcc gcacaaacga aggtctcact
     1561 taatcttctg tactctgaag aggagtggga aataccaaga aaaacatcaa actcgaatga
     1621 ttttcccaaa cccctaccac aagatattca tcagctgcga gataggctga tcaggagcaa
     1681 gctcgtacga gaagaaacaa aatgacaaaa aaaatcctat actatatagg ttacaaataa
     1741 aaaagtatca aaaatgaagc ctgcatctct caggcaaatg gcattctgac atcctctttt
     1801 agaatctagc aagaccggtc ttctcgtaag tgcccaactt gaactgagga acagtcatgt
     1861 ctaaggctac aaactcaatg atgatgatga tgatggtcga cggcgctatt cagatcctct
     1921 tctgagatga gtttttgttc tagaaagctg gcggccgccg cggctcgagg taccgatccg
     1981 agacggccgg ctgggccacg tgaattcctg cagcttcagc ctctcttttc tcgagagata
     2041 ccccttcttc tttagcagca atgctggcaa tagtagtatt tataaacaat aacccgttat
     2101 ttgtgctgtt ggaaaatggc aaaacagcaa catcgaaatc cccttctaaa tctgagtaac
     2161 cgatgacagc ttcagccgga atttgtgccg tttcatcttc tgttgtagtg ttgactggag
     2221 cagctaatgc ggaggatgct gcgaataaaa cagcagtaaa aattgaagga aatctcatcg
     2281 tttcgaataa ttagttgttt tttgatcttc tcaagttgtc gttaaaagtc gttaaaatca
     2341 aaagcttgtc aattggaacc agtcgcaatt atgaaagtaa gctaataatg atgataaaaa
     2401 aaaaggttta agacagggca gcttccttct gtttatatat tgctgtcaag taggggttag
     2461 aacagttaaa ttttgatcat gaacgttagg ctatcagcag tattcccacc agaatcttgg
     2521 aagcatacaa tgtggagaca atgcataatc atccaaaaag cgggtgtttc cccatttgcg
     2581 tttcggcaca ggtgcaccgg ggttcagaag cgatagagag actgcgctaa gcattaatga
     2641 gattattttt gagcattcgt caatcaatac caaacaagac aaacggtatg ccgacttttg
     2701 gaagtttctt tttgaccaac tggccgttag catttcaacg aaccaaactt agttcatctt
     2761 ggatgagatc acgcttttgt catattaggt tccaagacag cgtttaaact gtcagttttg
     2821 ggccatttgg ggaacatgaa actatttgac cccacactca gaaagccctc atctggagtg
     2881 atgttcgggt gtaatgcgga gcttgttgca ttcggaaata aacaaacatg aacctcgcca
     2941 ggggggccag gatagacagg ctaataaagt catggtgtta gtagcctaat agaaggaatt
     3001 ggaatgagcg agctccaatc aagcccaata actgggctgg tttttcgatg gcaaaagtgg
     3061 gtgttgagga gaagaggagt ggaggtcctg cgtttgcaac ggtctgctgc tagtgtatcc
     3121 cctcctgttg cgtttggcac ttatgtgtga gaatggacct gtggatgtcg gatggcaaaa
     3181 agagatctcg gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     3241 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     3301 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     3361 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     3421 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     3481 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     3541 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     3601 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     3661 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     3721 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     3781 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     3841 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     3901 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     3961 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     4021 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     4081 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     4141 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     4201 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     4261 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     4321 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     4381 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     4441 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     4501 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     4561 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     4621 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     4681 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     4741 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     4801 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     4861 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     4921 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     4981 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     5041 tactcatact cttccttttt caat
//
