LOCUS       40924_6576        4518 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Phagemid vector pBK-CMV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Marsh S.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-1995) Marketing Analysis, Stratagene, 11011 North 
            Torrey Pines Road, La Jolla, CA 92037, USA
REFERENCE   2  (bases 1 to 4518)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4518)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (04-OCT-1995) Marketing Analysis, Stratagene, 11011 North Torrey 
            Pines Road, La Jolla, CA 92037, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4518
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..462
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    complement(469..590)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     952..968
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        975..993
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1015..1122
                     /label=multiple cloning site
                     /note="multiple cloning site"
     promoter        complement(1140..1158)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1179..1195)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1203..1219)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1328..1531)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(1532..1835)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(2010..2598)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    complement(2927..2974)
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(3209..4000)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(4035..4392)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        complement(4394..4498)
                     /label=AmpR promoter
ORIGIN
        1 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
       61 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      121 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      181 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
      241 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
      301 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
      361 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
      421 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttacgcgtta agatacattg
      481 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
      541 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
      601 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
      661 aaaacctcta caaatgtggt atggctgatt atgatcatga acagactgtg aggactgagg
      721 ggcctgaaat gagccttggg actgtgaatc taaaatacac aaacaattag aatcagtagt
      781 ttaacacatt atacacttaa aaattggatc tccattcgcc attcaggctg cgcaactgtt
      841 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
      901 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
      961 cggccagtga attgtaatac gactcactat agggcgaatt gggtacactt acctggtacc
     1021 ccacccgggt ggaaaatcga tgggcccgcg gccgctctag aagtactctc gagaagcttt
     1081 ttgaattctt tggatccact agtgtcgacc tgcaggcgcg cgagctccag cttttgttcc
     1141 ctttagtgag ggttaatttc gagcttggcg taatcaaggt catagctgtt tcctgtgtga
     1201 aattgttatc cgctcacaat tccacacaat atacgagccg gaagtataaa gtgtaaagcc
     1261 tggggtgcct aatgagtgag ctaactcaca gtaattgcgg ctagcggatc tgacggttca
     1321 ctaaaccagc tctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca
     1381 atggggcgga gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac
     1441 tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac
     1501 gcccattgat gtactgccaa aaccgcatca ccatggtaat agcgatgact aatacgtaga
     1561 tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc
     1621 atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg
     1681 ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg
     1741 gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc
     1801 agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat gggctatgaa
     1861 ctaatgaccc cgtaattgat tactattaat aactaatgca tggcggtaat acggttatcc
     1921 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     1981 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     2041 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     2101 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     2161 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     2221 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     2281 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     2341 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     2401 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     2461 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     2521 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     2581 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     2641 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     2701 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaacctgag
     2761 gctatggcag ggcctgccgc cccgacgttg gctgcgagcc ctgggccttc acccgaactt
     2821 ggggggtggg gtggggaaaa ggaagaaacg cgggcgtatt ggccccaatg gggtctcggt
     2881 ggggtatcga cagagtgcca gccctgggac cgaaccccgc gtttatgaac aaacgaccca
     2941 acaccgtgcg ttttattctg tctttttatt gccgtcatag cgcgggttcc ttccggtatt
     3001 gtctccttcc gtgtttcagt tagcctcccc ctagggtggg cgaagaactc cagcatgaga
     3061 tccccgcgct ggaggatcat ccagccggcg tcccggaaaa cgattccgaa gcccaacctt
     3121 tcatagaagg cggcggtgga atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg
     3181 gtcatttcga accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga
     3241 tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc
     3301 cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca
     3361 cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg
     3421 gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg ctcgccttga
     3481 gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat
     3541 cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt
     3601 cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg
     3661 atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca
     3721 atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc
     3781 ccgtcgtggc cagccacgat agccgcgctg cctcgtcttg cagttcattc agggcaccgg
     3841 acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg
     3901 catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag
     3961 cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg
     4021 tctcttgatc gatctttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg
     4081 aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca
     4141 tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc
     4201 gggactatgg ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag
     4261 cctggggact ttccacacct ggttgctgac taattgagat gcatgctttg catacttctg
     4321 cctgctgggg agcctgggga ctttccacac cctaactgac acacattcca cagctggttc
     4381 tttccgcctc aggactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     4441 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     4501 catttccccg aaaagtgc
//
