LOCUS       40924_6561        7028 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Corynebacterium glutamicum/Escherichia coli shuttle expression 
            vector pBJ1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7028)
  AUTHORS   Equbal MJ, Deb JK, Agarwal GP.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   2  (bases 1 to 7028)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7028)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (28-FEB-2010) Biochemical Engineering "
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7028
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(26..73)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(140..157)
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_feature    complement(158..203)
                     /label=multiple cloning site
                     /note="multiple cloning site"
     CDS             complement(207..239)
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
     CDS             complement(243..260)
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
     CDS             complement(270..287)
                     /label=6xHis
                     /note="6xHis affinity tag"
     RBS             complement(306..328)
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(343..367)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(368..386)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        695..772
                     /label=lacI promoter
     misc_feature    1724..4474
                     /note="Corynebacterium glutamicum plasmid pBL1 replicon; 
                     involved in replication in Corynebacterium glutamicum"
     misc_feature    4616..4758
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4944..5532)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             5654..6466
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(6562..7017)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
      181 cgacggagct cgaattcgga tccgcgaccc atttgctgtc caccagtcat gctagccata
      241 tggctgccgc gcggcaccag gccgctgctg tgatgatgat gatgatggct gctgcccatg
      301 gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca
      361 caattcccct atagtgagtc gtattaattt cgcgggatcg agatctcgat cctctacgcc
      421 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
      481 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
      541 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca
      601 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
      661 caggagtcgc ataagggaga gcgtcgagat cccggacacc atcgaatggc gcaaaacctt
      721 tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga atgtgaaacc
      781 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt
      841 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc
      901 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct
      961 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat
     1021 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg
     1081 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat
     1141 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt
     1201 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca
     1261 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc
     1321 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa
     1381 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat
     1441 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct
     1501 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg
     1561 cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat
     1621 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg
     1681 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagaacaaca agacccatca
     1741 tagtttgccc ccgcgacatt gaccataaat tcatcgcaca aaatatcgaa cggggtttat
     1801 gccgctttta gtgggtgcga agaatagtct gctcattacc cgcgaacacc gccgcattca
     1861 gatcacgctt agtagcgtcc ccatgagtag gcagaaccgc gtccaagtcc acatcatcca
     1921 taacgatcat gcacggggtg gaatccacac ccagacttgc cagcacctca ttagcgacac
     1981 gttgcgcagc ggccacgtcc ttagccttat ccacgcaatc tagaacgtac tgcctaaccg
     2041 cgaaatcaga ctgaatcagt ttccaatcat cgggcttcac caaagcaaca gcaacgcggg
     2101 ttgattcgac ccgttccggt gcttccagac cggcgagctt gtacagttct tcttccattt
     2161 cacgacgtac atcagcgtct atgtaatcaa tgcccaaagc acgcttagcc ccacgtgacc
     2221 aggacgaacg caggttttta gaaccaacct catactcacg ccaccgagcc accaaaacag
     2281 cgtccatatc ctcgccggcg tcgctttgat cggccaacat atccaacatc tgaaacggcg
     2341 tgtacgaccc cttagacgcg gttttagtag cggagccagt cagttcctga gacatgccct
     2401 tagcgaggta ggttgccatt ttcgcagcgt ctccacccca ggtagacacc tgatcaagtt
     2461 tgaccccgtg ctcacgcagt ggcgcgtcca taccggcctt aaccacacca gcagaccagc
     2521 gggaaaacat ggaatcctca aacgccttga gttcatcgtc agacagtgga cgatccaaga
     2581 acaacagcat gttgcggtgc aagtgccaac cgttcgccca agagtctgtg acctcatagt
     2641 cactataggt gtgctccacc ccgtaccgtg cacgttcttt cttccactga gatgttttca
     2701 ccatcgaaga gtacgcagtc ttaatacccg cttcaacctg cgcaaatgac tgtgagcggt
     2761 tgtgtcgaac agtgcccaca aacatcatga gcgcgccacc cgccgccaag tgattcttag
     2821 tagcaatagc cagctcaatg cggcgttcgc ccatgacttc caattcagcc agaggtgacc
     2881 cccagcgaga gtgagagttt tgcagaccct caaactgcga agcaccgtta gacgaccagg
     2941 acaccgcaac agcttcgtcc ctgcgccacc tatggcaccc cgccagagcc ttactattgg
     3001 tgatcttgta catgacgttt tgcctacgcc acgccctagc gcgagtgacc ttagaaccct
     3061 cattgacctg cggttcctta gaggtgttca cttctatttc agtgttactc agtgttacct
     3121 agacccgatg ttgtgcgggg ttgcgcagtg cgagtttgtg cgggtgttgt gcccgttgtc
     3181 ttagctagtg ctatggttgt caattgaaac cccttcgggt tatgtggccc ccgtgcatat
     3241 gagttggtag ctcgcacggg ggtttgtctt gtctagggac tattaatttt tagtggtgtt
     3301 tggtggccgc ctagcttggc tatgcgtgcc agcttacccg tactcaatgt taaagatttg
     3361 catcgacatg ggagggttac gtgtccgata cctagggggg gtatccgcga ctaggtgccc
     3421 cggtgctcac tgtctgtacc ggcggggcaa gccccacacc ccgcatggac agggtggctc
     3481 cgccccctgc acccccagca atctgcatgt acatgtttta cacattagca cgacatgact
     3541 gcatgtgcat gcactgcatg cagactaggt aaatatgagt atgtacgact agtaacagga
     3601 gcactgcaca taatgaatga gttgcaggac aatgtttgct acgcatgcgc atgacatatc
     3661 gcaggaaagc tactagagtc ttaaagcatg gcaaccaagg cacagctaga acagcaacta
     3721 caagaagctc aacaggcact acaggcgcag caagcgcagg cacaagccac catcgaagca
     3781 ctagaagcgc aggcaaaggc taagcccgtc gtggtcaccg cacgcgttcc tttggcacta
     3841 cgtgaggaca tgaagcgcgc aggcatgcag aacggtgaaa acctccaaga gttcatgatc
     3901 gccgcgttta ccgagcggct agaaaagctc accaccaccg acaacgagga aaacaatgtc
     3961 taacccacta gttctctttg cccaccgtga cccggtaaat gacgtgacgt tcgagtgcat
     4021 tgagcacgcc acctacgaca cactttcaca cgctaaagac cagatcaccg cccaaatgca
     4081 agccctagac gaagaagccg ccctactgcc ctaatgggtg tttcatgggt gtttccctag
     4141 tgtttcatgg tgttttcacc taagctaggg aattgcgcga gaagtctcgc aaaaatcagc
     4201 aacccccgga accacacagt tcacgggggt tcttctatgc cagaaatcag aaaggggaac
     4261 cagtgaacga ccccgaatat tggatcacag cgcagcaggt cgccgcccgc gtagctctca
     4321 ccccggccac cattaaaaag tgggcaaacg agggaaaaat caccgcatac aagatcggca
     4381 agtccgtccg attcaaagca tcagacgtag acaagcttgc cacaccccga gacgcagcct
     4441 agaagccatt ctaagcgact atctagcccc ggttctgcct cgcgcgtttc ggtgatgacg
     4501 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     4561 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
     4621 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga
     4681 gcagattgta ctgagagtgc accatatatg cggtgtgaaa taccgcacag atgcgtaagg
     4741 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     4801 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     4861 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     4921 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     4981 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     5041 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     5101 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     5161 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     5221 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     5281 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     5341 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     5401 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     5461 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     5521 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     5581 aactcacgtt aagggatttt ggtcatgaac aataaaactg tctgcttaca taaacagtaa
     5641 tacaaggggt gttatgagcc atattcaacg ggaaacgtct tgctctaggc cgcgattaaa
     5701 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
     5761 aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
     5821 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
     5881 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
     5941 actcaccact gcgatccccg ggaaaacagc attccaggta ttagaagaat atcctgattc
     6001 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt
     6061 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat
     6121 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
     6181 acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca
     6241 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga
     6301 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct
     6361 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc
     6421 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaag aattaattca
     6481 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     6541 ttccccgaaa agtgccacct gaaattgtaa acgttaatat tttgttaaaa ttcgcgttaa
     6601 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     6661 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     6721 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     6781 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     6841 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     6901 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     6961 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     7021 attcgcca
//
