LOCUS       62056_4045        7446 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7446)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1083..1523
                     /codon_start=1
                     /label=GAL4 DNA binding domain
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVA"
     misc_feature    2306..2361
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     polyA_signal    complement(2466..2587)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2832..3189
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3240..4172
                     /codon_start=1
                     /label=hRluc
                     /note="Renilla luciferase"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             4224..5012
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    5079..5127
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     promoter        5283..5387
                     /label=AmpR promoter
     CDS             5388..6245
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      complement(6406..6994)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    7110..7393
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to
                     maintain plasmids as monomers"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagccag cttgaagcaa gcctcctgaa
     1081 agatgaagct actgtcttct atcgaacaag catgcgatat ttgccgactt aaaaagctca
     1141 agtgctccaa agaaaaaccg aagtgcgcca agtgtctgaa gaacaactgg gagtgtcgct
     1201 actctcccaa aaccaaaagg tctccgctga ctagggcaca tctgacagaa gtggaatcaa
     1261 ggctagaaag actggaacag ctatttctac tgatttttcc tcgagaagac cttgacatga
     1321 ttttgaaaat ggattcttta caggatataa aagcattgtt aacaggatta tttgtacaag
     1381 ataatgtgaa taaagatgcc gtcacagata gattggcttc agtggagact gatatgcctc
     1441 taacattgag acagcataga ataagtgcga catcatcatc ggaagagagt agtaacaaag
     1501 gtcaaagaca gttgactgta gcgattccgt cgacaccacc tactccctct ccagcgatcg
     1561 ccgaagagtg ggatctgatc cacattgcca cagaggccca tcgcagcacc aatgcccagg
     1621 gcagccattg gaaacagagg cggaaattcc tgcccgatga cattggccag tcacccattg
     1681 tctccatgcc ggacggagac aaggtggacc tggaagcctt cagcgagttt accaagatca
     1741 tcaccccggc catcacccgt gtggtggact ttgccaaaaa actgcccatg ttctccgagc
     1801 tgccttgcga agaccagatc atcctcctga aggggtgctg catggagatc atgtccctgc
     1861 gggcggctgt ccgctacgac cctgagagcg acaccctgac gctgagtggg gagatggctg
     1921 tcaagcggga gcagctcaag aatggcggcc tgggcgtagt ctccgacgcc atctttgaac
     1981 tgggcaagtc actctctgcc tttaacctgg atgacacgga agtggctctg ctgcaggctg
     2041 tgctgctaat gtcaacagac cgctcgggcc tgctgtgtgt ggacaagatc gagaagagtc
     2101 aggaggcgta cctgctggcg ttcgagcact acgtcaacca ccgcaaacac aacattccgc
     2161 acttctggcc caagctgctg atgaaggaga gagaagtgca gagttcgatt ctgtacaagg
     2221 gggcagcggc agaaggccgg ccgggcgggt cactgggcgt ccacccggaa ggacagcagc
     2281 ttctcggaat gcatgttgtt taaacgaatt cgggctcggt acccggggat cctctagagt
     2341 cgacctgcag gcatgcaagc tgatccggct gctaacaaag cccgaaagga agctgagttg
     2401 gctgctgcca ccgctgagca ataactagca taaccccttg gggcggccgc ttcgagcaga
     2461 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     2521 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     2581 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg agatgtggga
     2641 ggttttttta agcaagtaaa acctctacaa atgtggtaaa atcgaatttt aacaaaatat
     2701 taacgcttac aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     2761 ccgcatacgc ggatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg
     2821 ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     2881 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     2941 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     3001 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     3061 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     3121 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     3181 ttttgcaaaa agcttgattc ttctgacaca acagtctcga accaaaggct ggagccacca
     3241 tggcttccaa ggtgtacgac cccgagcaac gcaaacgcat gatcactggg cctcagtggt
     3301 gggctcgctg caagcaaatg aacgtgctgg actccttcat caactactat gattccgaga
     3361 agcacgccga gaacgccgtg atttttctgc atggtaacgc tgcctccagc tacctgtgga
     3421 ggcacgtcgt gcctcacatc gagcccgtgg ctagatgcat catccctgat ctgatcggaa
     3481 tgggtaagtc cggcaagagc gggaatggct catatcgcct cctggatcac tacaagtacc
     3541 tcaccgcttg gttcgagctg ctgaaccttc caaagaaaat catctttgtg ggccacgact
     3601 ggggggcttg tctggccttt cactactcct acgagcacca agacaagatc aaggccatcg
     3661 tccatgctga gagtgtcgtg gacgtgatcg agtcctggga cgagtggcct gacatcgagg
     3721 aggatatcgc cctgatcaag agcgaagagg gcgagaaaat ggtgcttgag aataacttct
     3781 tcgtcgagac catgctccca agcaagatca tgcggaaact ggagcctgag gagttcgctg
     3841 cctacctgga gccattcaag gagaagggcg aggttagacg gcctaccctc tcctggcctc
     3901 gcgagatccc tctcgttaag ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca
     3961 acgcctacct tcgggccagc gacgatctgc ctaagatgtt catcgagtcc gaccctgggt
     4021 tcttttccaa cgctattgtc gagggagcta agaagttccc taacaccgag ttcgtgaagg
     4081 tgaagggcct ccacttcagc caggaggacg ctccagatga aatgggtaag tacatcaaga
     4141 gcttcgtgga gcgcgtgctg aagaacgagc agaccggtgg tgggagcgga ggtggcggat
     4201 caggtggcgg aggctccgga gggattgaac aagatggatt gcacgcaggt tctccggccg
     4261 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     4321 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     4381 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     4441 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     4501 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     4561 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     4621 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     4681 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     4741 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     4801 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     4861 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     4921 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     4981 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     5041 cgaccaagcg acgcccaacc tgccatcacg atggccgcaa taaaatatct ttattttcat
     5101 tacatctgtg tgttggtttt ttgtgtgaat cgatagcgat aaggatcctc tttgcgcttg
     5161 cgttttccct tgtccagata gcccagtagc tgacattcat ccggggtcag caccgtttct
     5221 gcggactggc tttctacgta atggtttctt agacgtcagg tggcactttt cggggaaatg
     5281 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     5341 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     5401 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     5461 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     5521 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     5581 caatgatgag cactttcaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     5641 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     5701 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5761 taaccatgag tgataacact gcggccaact tacttctgac aactatcgga ggaccgaagg
     5821 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     5881 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     5941 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     6001 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     6061 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     6121 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     6181 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     6241 attggtaatt cgaaatgacc gaccaagcga cgcccaaccg gtatcagctc actcaaaggc
     6301 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     6361 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     6421 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6481 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6541 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6601 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6661 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     6721 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     6781 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     6841 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     6901 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     6961 gcagcagatt acgcgcagaa aaaaaggatt tcaagaagat cctttgatct tttctacggg
     7021 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     7081 aaggatcttc acctagatcc ttttatagtc cggaaataca ggaacgcacg ctggatggcc
     7141 cttcgctggg atggtgaaac catgaaaaat ggcagcttca gtggattaag tgggggtaat
     7201 gtggcctgta ccctctggtt gcataggtat tcatacggtt aaaatttatc aggcgcgatt
     7261 gcggcagttt ttcgggtggt ttgttgccat ttttacctgt ctgctgccgt gatcgcgctg
     7321 aacgcgtttt agcggtgcgt acaattaagg gattatggta aatccactta ctgtctgccc
     7381 tcgtagccat cgagataaac cgcagtactc cggccacgat gcgtccggcg tagaggatcg
     7441 agatct
//
