LOCUS       pBIND-ER(alpha).        7605 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Renilla luciferase vector encoding the Gal4 DNA-binding domain fused
            to an estrogen receptor ligand-binding domain.
ACCESSION   .
VERSION     .
KEYWORDS    pBIND-ER(alpha)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7605)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7605)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7605
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1083..1523
                     /label=GAL4 DNA binding domain
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     CDS             1527..1553
                     /codon_start=1
                     /product="peptide derived from a human Ig alpha chain"
                     /label=peptide derived from a human Ig alpha chain
                     /note="IgA linker"
                     /translation="PSTPPTPSP"
     CDS             1563..2453
                     /label=ER-LBD
                     /note="estrogen nuclear receptor alpha ligand-binding
                     domain"
     misc_feature    2465..2520
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     polyA_signal    complement(2625..2746)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2991..3348
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3399..4331
                     /label=hRluc
                     /note="Renilla luciferase"
     CDS             4383..5171
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5238..5286
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     promoter        5442..5546
                     /label=AmpR promoter
     CDS             5547..6404
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      complement(6565..7153)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    7269..7552
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to
                     maintain plasmids as monomers"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagccag cttgaagcaa gcctcctgaa
     1081 agatgaagct actgtcttct atcgaacaag catgcgatat ttgccgactt aaaaagctca
     1141 agtgctccaa agaaaaaccg aagtgcgcca agtgtctgaa gaacaactgg gagtgtcgct
     1201 actctcccaa aaccaaaagg tctccgctga ctagggcaca tctgacagaa gtggaatcaa
     1261 ggctagaaag actggaacag ctatttctac tgatttttcc tcgagaagac cttgacatga
     1321 ttttgaaaat ggattcttta caggatataa aagcattgtt aacaggatta tttgtacaag
     1381 ataatgtgaa taaagatgcc gtcacagata gattggcttc agtggagact gatatgcctc
     1441 taacattgag acagcataga ataagtgcga catcatcatc ggaagagagt agtaacaaag
     1501 gtcaaagaca gttgactgta gcgattccgt cgacaccacc tactccctct ccagcgatcg
     1561 ccaaacgctc taagaagaac agcctggcct tgtccctgac ggccgaccag atggtcagtg
     1621 ccttgttgga tgctgagccc ccgatactct attccgagta tgatcctacc agacccttca
     1681 gtgaagcttc gatgatgggc ttactgacca acctggcaga cagggagctg gttcacatga
     1741 tcaactgggc gaagagggtg ccaggctttg tggatttgac cctccatgat caggtccacc
     1801 ttctagaatg tgcctggcta gagatcctga tgattggtct cgtctggcgc tccatggagc
     1861 acccagggaa gctactgttt gctcctaact tgctcttgga caggaaccag ggaaaatgtg
     1921 tagagtgcat ggtggagatc ttcgacatgc tgctggctac atcatctcgg ttccgcatga
     1981 tgaatctgca gggagaggag tttgtgtgcc tcaaatctat tattttgctt aattctggag
     2041 tgtacacatt tctgtccagc accctgaagt ctctggaaga gaaggaccat atccaccgag
     2101 tcctggacaa gatcacagac actttgatcc acctgatggc caaggcaggc ctgaccctgc
     2161 agcagcagca ccagcggctg gcccagctcc tcctcatcct ctcccacatc aggcacatga
     2221 gtaacaaagg catggagcat ctgtacagca tgaagtgcaa gaacgtggtg cccctctatg
     2281 acctgctgct ggagatgctg gacgcccacc gcctacatgc gcccactagc cgtggagggg
     2341 catccgtgga ggagacggac caaagccact tggccactgc gggctctact tcatcgcatt
     2401 ccttgcaaaa gtattacatc acgggggagg cagagggttt ccctgccacg gtctgagttt
     2461 aaacgaattc gggctcggta cccggggatc ctctagagtc gacctgcagg catgcaagct
     2521 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
     2581 taactagcat aaccccttgg ggcggccgct tcgagcagac atgataagat acattgatga
     2641 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     2701 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     2761 cattcatttt atgtttcagg ttcaggggga gatgtgggag gtttttttaa gcaagtaaaa
     2821 cctctacaaa tgtggtaaaa tcgaatttta acaaaatatt aacgcttaca atttcctgat
     2881 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgcg gatctgcgca
     2941 gcaccatggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
     3001 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     3061 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     3121 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     3181 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     3241 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     3301 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttgattct
     3361 tctgacacaa cagtctcgaa ccaaaggctg gagccaccat ggcttccaag gtgtacgacc
     3421 ccgagcaacg caaacgcatg atcactgggc ctcagtggtg ggctcgctgc aagcaaatga
     3481 acgtgctgga ctccttcatc aactactatg attccgagaa gcacgccgag aacgccgtga
     3541 tttttctgca tggtaacgct gcctccagct acctgtggag gcacgtcgtg cctcacatcg
     3601 agcccgtggc tagatgcatc atccctgatc tgatcggaat gggtaagtcc ggcaagagcg
     3661 ggaatggctc atatcgcctc ctggatcact acaagtacct caccgcttgg ttcgagctgc
     3721 tgaaccttcc aaagaaaatc atctttgtgg gccacgactg gggggcttgt ctggcctttc
     3781 actactccta cgagcaccaa gacaagatca aggccatcgt ccatgctgag agtgtcgtgg
     3841 acgtgatcga gtcctgggac gagtggcctg acatcgagga ggatatcgcc ctgatcaaga
     3901 gcgaagaggg cgagaaaatg gtgcttgaga ataacttctt cgtcgagacc atgctcccaa
     3961 gcaagatcat gcggaaactg gagcctgagg agttcgctgc ctacctggag ccattcaagg
     4021 agaagggcga ggttagacgg cctaccctct cctggcctcg cgagatccct ctcgttaagg
     4081 gaggcaagcc cgacgtcgtc cagattgtcc gcaactacaa cgcctacctt cgggccagcg
     4141 acgatctgcc taagatgttc atcgagtccg accctgggtt cttttccaac gctattgtcg
     4201 agggagctaa gaagttccct aacaccgagt tcgtgaaggt gaagggcctc cacttcagcc
     4261 aggaggacgc tccagatgaa atgggtaagt acatcaagag cttcgtggag cgcgtgctga
     4321 agaacgagca gaccggtggt gggagcggag gtggcggatc aggtggcgga ggctccggag
     4381 ggattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     4441 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     4501 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     4561 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     4621 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     4681 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     4741 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     4801 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     4861 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     4921 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     4981 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     5041 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     5101 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     5161 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     5221 gccatcacga tggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt
     5281 tgtgtgaatc gatagcgata aggatcctct ttgcgcttgc gttttccctt gtccagatag
     5341 cccagtagct gacattcatc cggggtcagc accgtttctg cggactggct ttctacgtaa
     5401 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     5461 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     5521 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     5581 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     5641 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     5701 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc actttcaaag
     5761 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     5821 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     5881 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     5941 cggccaactt acttctgaca actatcggag gaccgaagga gctaaccgct tttttgcaca
     6001 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     6061 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     6121 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     6181 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     6241 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     6301 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     6361 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaattc gaaatgaccg
     6421 accaagcgac gcccaaccgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     6481 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     6541 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     6601 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     6661 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     6721 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     6781 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     6841 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     6901 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     6961 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     7021 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     7081 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     7141 aaaaggattt caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     7201 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     7261 tttatagtcc ggaaatacag gaacgcacgc tggatggccc ttcgctggga tggtgaaacc
     7321 atgaaaaatg gcagcttcag tggattaagt gggggtaatg tggcctgtac cctctggttg
     7381 cataggtatt catacggtta aaatttatca ggcgcgattg cggcagtttt tcgggtggtt
     7441 tgttgccatt tttacctgtc tgctgccgtg atcgcgctga acgcgtttta gcggtgcgta
     7501 caattaaggg attatggtaa atccacttac tgtctgccct cgtagccatc gagataaacc
     7561 gcagtactcc ggccacgatg cgtccggcgt agaggatcga gatct
//
