LOCUS       40924_6417        6479 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Reporter vector pBIC, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6479)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6479)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6479)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6479)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6479
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(4..138)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(563..583)
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          complement(713..778)
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     misc_feature    complement(867..911)
                     /label=3' UTR of LUC
                     /note="3' UTR of LUC"
     CDS             complement(915..2564)
                     /label=luciferase
                     /note="firefly luciferase"
     misc_feature    complement(2615..3060)
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the
                     foot-and-mouth disease virus"
     CDS             complement(3129..3785)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     regulatory      complement(3813..3921)
                     /label=HSV-TK promoter
                     /note="HSV-TK promoter"
                     /regulatory_class="promoter"
     promoter        complement(3952..3970)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(4222..4810)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4984..5841)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5842..5946)
                     /label=AmpR promoter
ORIGIN
        1 ggggatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
       61 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
      121 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
      181 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
      241 gatctctagt caaggcacta tacatcaaat attccttatt aaccccttta caaattaaaa
      301 agctaaaggt acacaatttt tgagcatagt tattaatagc agacactcta tgcctgtgtg
      361 gagtaagaaa aaacagtatg ttatgattat aactgttatg cctacttata aaggttacag
      421 aatatttttc cataattttc ttgtatagca gtgcagcttt ttcctttgtg gtgtaaatag
      481 caaagcaagc aagagttcta ttactaaaca cagcatgact caaaaaactt agcaattctg
      541 aaggaaagtc cttggggtct tctacctttc tcttcttttt tggaggagta gaatgttgag
      601 agtcagcagt agcctcatca tcactagatg gcatttcttc tgagcaaaac aggttttcct
      661 cattaaaggc attccaccac tgctcccatt catcagttcc ataggttgga atctaaaata
      721 cacaaacaat tagaatcagt agtttaacac attatacact taaaaatttt atatttacct
      781 tagagcttta aatctctgta ggtagtttgt ccaattatgt cacaccacag aagtaaggtt
      841 ccttcacaaa gagatcctct agagtcatta caatagctaa gaatttcgtc atcgctgaat
      901 acagttacat tttacaattt ggactttccg cccttcttgg cctttatgag gatctctctg
      961 atttttcttg cgtcgagttt tccggtaaga cctttcggta cttcgtccac aaacacaact
     1021 cctccgcgca actttttcgc ggttgttact tgactggcga cgtaatccac gatctctttt
     1081 tccgtcatcg tctttccgtg ctccaaaaca acaacggcgg cgggaagttc accggcgtca
     1141 tcgtcgggaa gacctgccac gcccgcgtcg aagatgttgg ggtgttgtaa caatatcgat
     1201 tccaattcag cgggggccac ctgatatcct ttgtatttaa ttaaagactt caagcggtca
     1261 actatgaaga agtgttcgtc ttcgtcccag taagctatgt ctccagaatg tagccatcca
     1321 tccttgtcaa tcaaggcgtt ggtcgcttcc ggattgttta cataaccgga cataatcata
     1381 ggtcctctga cacataattc gcctctctga ttaacgccca gcgttttccc ggtatccaga
     1441 tccacaacct tcgcttcaaa aaatggaaca actttaccga ccgcgcccgg tttatcatcc
     1501 ccctcgggtg taatcagaat agctgatgta gtctcagtga gcccatatcc ttgtcgtatc
     1561 cctggaagat ggaagcgttt tgcaaccgct tccccgactt ctttcgaaag aggtgcgccc
     1621 ccagaagcaa tttcgtgtaa attagataaa tcgtatttgt caatcagagt gcttttggcg
     1681 aagaatgaaa atagggttgg tactagcaac gcactttgaa ttttgtaatc ctgaagggat
     1741 cgtaaaaaca gctcttcttc aaatctatac attaagacga ctcgaaatcc acatatcaaa
     1801 tatccgagtg tagtaaacat tccaaaaccg tgatggaatg gaacaacact taaaatcgca
     1861 gtatccggaa tgatttgatt gccaaaaata ggatctctgg catgcgagaa tctgacgcag
     1921 gcagttctat gcggaagggc cacaccctta ggtaacccag tagatccaga ggaattcatt
     1981 atcagtgcaa ttgttttgtc acgatcaaag gactctggta caaaatcgta ttcattaaaa
     2041 ccgggaggta gatgagatgt gacgaacgtg tacatcgact gaaatccctg gtaatccgtt
     2101 ttagaatcca tgataataat tttctggatt attggtaatt ttttttgcac gttcaaaatt
     2161 ttttgcaacc cctttttgga aacaaacact acggtaggct gcgaaatgtt catactgttg
     2221 agcaattcac gttcattata aatgtcgttc gcgggcgcaa ctgcaactcc gataaataac
     2281 gcgcccaaca ccggcataaa gaattgaaga gagttttcac tgcatacgac gattctgtga
     2341 tttgtattca gcccatatcg tttcatagct tctgccaacc gaacggacat ttcgaagtat
     2401 tccgcgtacg tgatgttcac ctcgatatgt gcatctgtaa aagcaattgt tccaggaacc
     2461 agggcgtatc tcttcatagc cttatgcagt tgctctccag cggttccatc ctctagagga
     2521 tagaatggcg ccgggccttt ctttatgttt ttggcgtctt ccattttacc aacagtaccg
     2581 gaatgccaag ctcgagctca gggtcattaa ttgtaaagga aaggtgccga cctccggtca
     2641 cctattcagg cgtagaagct ttttaaaccg agcgctttta tagaagcccc agtccccttc
     2701 tcagatcccg agtgtcgcgt gttacctcgg ggtacctgaa gggcatcctt agcctgtcac
     2761 cagtgtgtgg gtaccagttt caatgtcact ttaaagtggg tttcgcagta aagtcgccgt
     2821 gctggggttg cacacatgac gggcccgtgt gggcgtggct tttggccccg tgggtccttg
     2881 ttaccaagga ggagttccca cggccgtcat gctccgctac gaagcaacag tgctgttact
     2941 aacactcgcc agtggatcga gcgtggagcc aaacacagta caaagtgtta cccctctaga
     3001 cctggaaaga ccaggcggag tttcaagttg cacgttttgt gtcagttggg gaaacctgct
     3061 gagctctgcg gcacgctgtt gacgctgtta agcgggtcgc tgcagcaata actgccttaa
     3121 aaaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
     3181 ccgacatgga agccatcaca gacggcatga tgaacctgaa tcgccagcgg catcagcacc
     3241 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
     3301 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac
     3361 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     3421 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
     3481 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
     3541 agctcaccgt ctttcattgc catacggaat tccggatgag cattcatcag gcgggcaaga
     3601 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     3661 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
     3721 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
     3781 tccattttag cttctgcagg gtcgctcggt gttcgaggcc acacgcgtca ccttaatatg
     3841 cgaagtggac ctcggaccgc gccgccccga ctgcatctgc gtgttcgaat tcgccaatga
     3901 caagacgctg ggcggggttt gccggatcct ctagagtcga gcttgggtct ccctatagtg
     3961 agtcgtatta atttcgataa gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     4021 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     4081 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     4141 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     4201 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     4261 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     4321 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     4381 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     4441 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     4501 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     4561 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     4621 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     4681 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     4741 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     4801 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     4861 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     4921 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     4981 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     5041 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     5101 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     5161 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     5221 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     5281 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     5341 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     5401 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     5461 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     5521 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     5581 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     5641 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     5701 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     5761 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     5821 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     5881 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     5941 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     6001 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
     6061 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     6121 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     6181 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatcg acgctctccc
     6241 ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc
     6301 gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct
     6361 gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc
     6421 ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgcc
//
