LOCUS       40924_6412       12703 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pBIB-KAN, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12703)
  AUTHORS   Condori J, Medina-Bolivar F.
  TITLE     Vector for transient expression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12703)
  AUTHORS   Condori J, Medina-Bolivar F.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2010) Arkansas Biosciences Institute, Arkansas 
            State University, 504 University Loop East, Jonesboro, AK 72401, USA
REFERENCE   3  (bases 1 to 12703)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12703)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-MAR-2010) Arkansas Biosciences Institute, Arkansas State 
            University, 504 University Loop East, Jonesboro, AK 72401, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12703
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      6..616
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     mobile_element  complement(1317..2084)
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             2276..3067
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             3369..4514
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    6113..6137
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     6755..6771
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(6780..7032)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        7520..7703
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             7727..8515
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     terminator      8908..9160
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    9709..9730
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9745..9775
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9783..9799
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9807..9823
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      10000..10624
                     /label=oriV
                     /note="incP origin of replication"
     CDS             10715..11362
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     rep_origin      11536..11914
                     /label=derived from ColE1 gene
                     /note="derived from ColE1 gene"
     oriT            12046..12155
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             12188..12556
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
ORIGIN
        1 ccgggctggt tgccctcgcc gctgggctgg cggccgtcta tggccctgca aacgcgccag
       61 aaacgccgtc gaagccgtgt gcgagacacc gcggccgccg gcgttgtgga tacctcgcgg
      121 aaaacttggc cctcactgac agatgagggg cggacgttga cacttgaggg gccgactcac
      181 ccggcgcggc gttgacagat gaggggcagg ctcgatttcg gccggcgacg tggagctggc
      241 cagcctcgca aatcggcgaa aacgcctgat tttacgcgag tttcccacag atgatgtgga
      301 caagcctggg gataagtgcc ctgcggtatt gacacttgag gggcgcgact actgacagat
      361 gaggggcgcg atccttgaca cttgaggggc agagtgctga cagatgaggg gcgcacctat
      421 tgacatttga ggggctgtcc acaggcagaa aatccagcat ttgcaagggt ttccgcccgt
      481 ttttcggcca ccgctaacct gtcttttaac ctgcttttaa accaatattt ataaaccttg
      541 tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac gccgaagggg ggtgcccccc
      601 cttctcgaac cctcccggcc cgctaacgcg ggcctcccat ccccccaggg gctgcgcccc
      661 tcggccgcga acggcctcac cccaaaaatg gcagcgctgg cagtccttgc cattgccggg
      721 atcggggcag taacgggatg ggcgatcagc ccgagcgcga cgcccggaag cattgacgtg
      781 ccgcaggtgc tggcatcgac attcagcgac caggtgccgg gcagtgaggg cggcggcctg
      841 ggtggcggcc tgcccttcac ttcggccgtc ggggcattca cggacttcat ggcggggccg
      901 gcaattttta ccttgggcat tcttggcata gtggtcgcgg gtgccgtgct cgtgttcggg
      961 ggtgcgataa acccagcgaa ccatttgagg tgataggtaa gattataccg aggtatgaaa
     1021 acgagaattg gacctttaca gaattactct atgaagcgcc atatttaaaa agctaccaag
     1081 acgaagagga tgaagaggat gaggaggcag attgccttga atatattgac aatactgata
     1141 agataatata tcttttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc
     1201 ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga
     1261 ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgggta
     1321 atgactccaa cttattgata gtgttttatg ttcagataat gcccgatgac tttgtcatgc
     1381 agctccaccg attttgagaa cgacagcgac ttccgtccca gccgtgccag gtgctgcctc
     1441 agattcaggt tatgccgctc aattcgctgc gtatatcgct tgctgattac gtgcagcttt
     1501 cccttcaggc gggattcata cagcggccag ccatccgtca tccatatcac cacgtcaaag
     1561 ggtgacagca ggctcataag acgccccagc gtcgccatag tgcgttcacc gaatacgtgc
     1621 gcaacaaccg tcttccggag actgtcatac gcgtaaaaca gccagcgctg gcgcgattta
     1681 gccccgacat agccccactg ttcgtccatt tccgcgcaga cgatgacgtc actgcccggc
     1741 tgtatgcgcg aggttaccga ctgcggcctg agttttttaa gtgacgtaaa atcgtgttga
     1801 ggccaacgcc cataatgcgg gctgttgccc ggcatccaac gccattcatg gccatatcaa
     1861 tgattttctg gtgcgtaccg ggttgagaag cggtgtaagt gaactgcagt tgccatgttt
     1921 tacggcagtg agagcagaga tagcgctgat gtccggcggt gcttttgccg ttacgcacca
     1981 ccccgtcagt agctgaacag gagggacagc tgatagacac agaagccact ggagcacctc
     2041 aaaaacacca tcatacacta aatcagtaag ttggcagcat cacccataat tgtggtttca
     2101 aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt gaaaaagctg
     2161 ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc gtcttgttat
     2221 aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat aataaatggc
     2281 taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga
     2341 tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata
     2401 tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga
     2461 catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca
     2521 tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta
     2581 tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt
     2641 tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga
     2701 attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagaaga
     2761 cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga
     2821 ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa
     2881 agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc
     2941 cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg agctattttt
     3001 tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga
     3061 attgttttag tacctagatg tggcgcaacg atgccggcga caagcaggag cgcaccgact
     3121 tcttccgcat caagtgtttt ggctctcagg ccgaggccca cggcaagtat ttgggcaagg
     3181 ggtcgctggt attcgtgcag ggcaagattc ggaataccaa gtacgagaag gacggccaga
     3241 cggtctacgg gaccgacttc attgccgata aggtggatta tctggacacc aaggcaccag
     3301 gcgggtcaaa tcaggaataa gggcacattg ccccggcgtg agtcggggca atcccgcaag
     3361 gagggtgaat gaatcggacg tttgaccgga aggcatacag gcaagaactg atcgacgcgg
     3421 ggttttccgc cgaggatgcc gaaaccatcg caagccgcac cgtcatgcgt gcgccccgcg
     3481 aaaccttcca gtccgtcggc tcgatggtcc agcaagctac ggccaagatc gagcgcgaca
     3541 gcgtgcaact ggctccccct gccctgcccg cgccatcggc cgccgtggag cgttcgcgtc
     3601 gtctcgaaca ggaggcggca ggtttggcga agtcgatgac catcgacacg cgaggaacta
     3661 tgacgaccaa gaagcgaaaa accgccggcg aggacctggc aaaacaggtc agcgaggcca
     3721 agcaggccgc gttgctgaaa cacacgaagc agcagatcaa ggaaatgcag ctttccttgt
     3781 tcgatattgc gccgtggccg gacacgatgc gagcgatgcc aaacgacacg gcccgctctg
     3841 ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc gctgcaaaac aaggtcattt
     3901 tccacgtcaa caaggacgtg aagatcacct acaccggcgt cgagctgcgg gccgacgatg
     3961 acgaactggt gtggcagcag gtgttggagt acgcgaagcg cacccctatc ggcgagccga
     4021 tcaccttcac gttctacgag ctttgccagg acctgggctg gtcgatcaat ggccggtatt
     4081 acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac ggcgatgggc ttcacgtccg
     4141 accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg cttccgcgtc ctggaccgtg
     4201 gcaagaaaac gtcccgttgc caggtcctga tcgacgagga aatcgtcgtg ctgtttgctg
     4261 gcgaccacta cacgaaattc atatgggaga agtaccgcaa gctgtcgccg acggcccgac
     4321 ggatgttcga ctatttcagc tcgcaccggg agccgtaccc gctcaagctg gaaaccttcc
     4381 gcctcatgtg cggatcggat tccacccgcg tgaagaagtg gcgcgagcag gtcggcgaag
     4441 cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc ctgggtcaat gatgacctgg
     4501 tgcattgcaa acgctagggc cttgtggggt cagttccggc tgggggttca gcagccagcg
     4561 ctttactggc atttcaggaa caagcgggca ctgctcgacg cacttgcttc gctcagtatc
     4621 gctcgggacg cacggcgcgc tctacgaact gccgataaac agaggattaa aattgacaat
     4681 tgtgattaag gctcagattc gacggcttgg agcggccgac gtgcaggatt tccgcgagat
     4741 ccgattgtcg gccctgaaga aagctccaga gatgttcggg tccgtttacg agcacgagga
     4801 gaaaaagccc atggaggcgt tcgctgaacg gttgcgagat gccgtggcat tcggcgccta
     4861 catcgacggc gagatcattg ggctgtcggt cttcaaacag gaggacggcc ccaaggacgc
     4921 tcacaaggcg catctgtccg gcgttttcgt ggagcccgaa cagcgaggcc gaggggtcgc
     4981 cggtatgctg ctgcgggcgt tgccggcggg tttattgctc gtgatgatcg tccgacagat
     5041 tccaacggga atctggtgga tgcgcatctt catcctcggc gcacttaata tttcgctatt
     5101 ctggagcttg ttgtttattt cggtctaccg cctgccgggc ggggtcgcgg cgacggtagg
     5161 cgctgtgcag ccgctgatgg tcgtgttcat ctctgccgct ctgctaggta gcccgatacg
     5221 attgatggcg gtcctggggg ctatttgcgg aactgcgggc gtggcgctgt tggtgttgac
     5281 accaaacgca gcgctagatc ctgtcggcgt cgcagcgggc ctggcggggg cggtttccat
     5341 ggcgttcgga accgtgctga cccgcaagtg gcaacctccc gtgcctctgc tcacctttac
     5401 cgcctggcaa ctggcggccg gaggacttct gctcgttcca gtagctttag tgtttgatcc
     5461 gccaatcccg atgcctacag gaaccaatgt tctcggcctg gcgtggctcg gcctgatcgg
     5521 agcgggttta acctacttcc tttggttccg ggggatctcg cgactcgaac ctacagttgt
     5581 ttccttactg ggctttctca gccccagatc tggggtcgat cagccgggga tgcatcaggc
     5641 cgacagtcgg aacttcgggt ccccgacctg taccattcgg tgagcaatgg ataggggagt
     5701 tgatatcgtc aacgttcact tctaaagaaa tagcgccact cagcttcctc agcggcttta
     5761 tccagcgatt tcctattatg tcggcatagt tctcaagatc gacagcctgt cacggttaag
     5821 cgagaaatga ataagaaggc tgataattcg gatctctgcg agggagatga tatttgatca
     5881 caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt
     5941 gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag
     6001 tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat
     6061 cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga
     6121 tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt
     6181 taatgtactg gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc
     6241 accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga
     6301 aaatcctgtt tgatggtggt tccgaaatcg gcaaaatccc ttataaatca aaagaatagc
     6361 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     6421 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     6481 cacccaaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     6541 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     6601 agaaagcgaa aggagcgggc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     6661 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     6721 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattcccg
     6781 atctagtaac atagatgaca ccgcgcgcga taatttatcc tagtttgcgc gctatatttt
     6841 gttttctatc gcgtattaaa tgtataattg cgggactcta atcataaaaa cccatctcat
     6901 aaataacgtc atgcattaca tgttaattat tacatgctta acgtaattca acagaaatta
     6961 tatgataatc atcgcaagac cggcaacagg attcaatctt aagaaacttt attgccaaat
     7021 gtttgaacga tcggggaaat tcgagctcgg tacccgggct ctagagtcga cctgcaggca
     7081 tgcaagcttc acgctgccgc aagcactcag ggcgcaaggg ctgctaaagg aagcggaaca
     7141 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta
     7201 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat
     7261 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg
     7321 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa
     7381 ggatctgatg gcgcagggga tcaagatcat gagcggagaa ttaagggagt cacgttatga
     7441 cccccgccga tgacgcggga caagccgttt tacgtttgga actgacagaa ccgcaacgtt
     7501 gaaggagcca ctcagccgcg ggtttctgga gtttaatgag ctaagcacat acgtcagaaa
     7561 ccattattgc gcgttcaaaa gtcgcctaag gtcactatca gctagcaaat atttcttgtc
     7621 aaaaatgctc cactgacgtt ccataaattc ccctcggtat ccaattagag tctcatattc
     7681 actctcaatc caaataatct gcaccggatc tggatcgttt cgcatgattg aacaagatgg
     7741 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     7801 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     7861 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
     7921 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     7981 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     8041 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     8101 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     8161 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     8221 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgatg atctcgtcgt
     8281 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     8341 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     8401 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     8461 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     8521 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     8581 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     8641 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccacgg gatctctgcg
     8701 gaacaggcgg tcgaaggtgc cgatatcatt acgacagcaa cggccgacaa gcacaacgcc
     8761 acgatcctga gcgacaatat gatcgggccc ggcgtccaca tcaacggcgt cggcggcgac
     8821 tgcccaggca agaccgagat gcaccgcgat atcttgctgc gttcggatat tttcgtggag
     8881 ttcccgccac agacccggat gatccccgat cgttcaaaca tttggcaata aagtttctta
     8941 agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt
     9001 aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt
     9061 agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag
     9121 gataaattat cgcgcgcggt gtcatctatg ttactagatc gggcctcctg tcaatgctgg
     9181 cggcggctct ggtggtggtt ctggtggcgg ctctgagggt ggtggctctg agggtggcgg
     9241 ttctgagggt ggcggctctg agggaggcgg ttccggtggt ggctctggtt ccggtgattt
     9301 tgattatgaa aagatggcaa acgctaataa gggggctatg accgaaaatg ccgatgaaaa
     9361 cgcgctacag tctgacgcta aaggcaaact tgattctgtc gctactgatt acggtgctgc
     9421 tatcgatggt ttcattggtg acgtttccgg ccttgctaat ggtaatggtg ctactggtga
     9481 ttttgctggc tctaattccc aaatggctca agtcggtgac ggtgataatt cacctttaat
     9541 gaataatttc cgtcaatatt taccttccct ccctcaatcg gttgaatgtc gcccttttgt
     9601 ctttggccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     9661 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     9721 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     9781 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct
     9841 ctagaggatc ccccgatgag ctaagctagc tatatcatca atttatgtat tacacataat
     9901 atcgcactca gtctttcatc tacggcaatg taccagctga tataatcagt tattgaaata
     9961 tttctgaatt taaacttgca tcaataaatt tatgtttttg cttggactat aatacctgac
    10021 ttgttatttt atcaataaat atttaaacta tatttctttc aagatgggaa ttggctgagt
    10081 ggctccttca acgttgcggt tctgtcagtt ccaaacgtaa aacggcttgt cccgcgtcat
    10141 cggcgggggt cataacgtga ctcccttaat tctccgctca tgatcagatt gtcgtttccc
    10201 gccttcagtt taaactatca gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa
    10261 agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc
    10321 catttgtatg tgcatgccaa ccacagggtt ccccagatct ggcgccggcc agcgagacga
    10381 gcaagattgg ccgccgcccg aaacgatccg acagcgcgcc cagcacaggt gcgcaggcaa
    10441 attgcaccaa cgcatacagc gccagcagaa tgccatagtg ggcggtgacg tcgttcgagt
    10501 gaaccagatc gcgcaggagg cccggcagca ccggcataat caggccgatg ccgacagcgt
    10561 cgagcgcgac agtgctcaga attacgatca ggggtatgtt gggtttcacg tctggcctcc
    10621 ggaccagcct ccgctggtcc gattgaacgc gcggattctt tatcactgat aagttggtgg
    10681 acatattatg tttatcagtg ataaagtgtc aagcatgaca aagttgcagc cgaatacagt
    10741 gatccgtgcc gccctggacc tgttgaacga ggtcggcgta gacggtctga cgacacgcaa
    10801 actggcggaa cggttggggg ttcagcagcc ggcgctttac tggcacttca ggaacaagcg
    10861 ggcgctgctc gacgcactgg ccgaagccat gctggcggag aatcatacgc attcggtgcc
    10921 gagagccgac gacgactggc gctcatttct gatcgggaat gcccgcagct tcaggcaggc
    10981 gctgctcgcc taccgcgatg gcgcgcgcat ccatgccggc acgcgaccgg gcgcaccgca
    11041 gatggaaacg gccgacgcgc agcttcgctt cctctgcgag gcgggttttt cggccgggga
    11101 cgccgtcaat gcgctgatga caatcagcta cttcactgtt ggggccgtgc ttgaggagca
    11161 ggccggcgac agcgatgccg gcgagcgcgg cggcaccgtt gaacaggctc cgctctcgcc
    11221 gctgttgcgg gccgcgatag acgccttcga cgaagccggt ccggacgcag cgttcgagca
    11281 gggactcgcg gtgattgtcg atggattggc gaaaaggagg ctcgttgtca ggaacgttga
    11341 aggaccgaga aagggtgacg attgatcagg accgctgccg gagcgcaacc cactcactac
    11401 agcagagcca tgtagacaac atcccctccc cctttccacc gcgtcagacg cccgtagcag
    11461 cccgctacgg gctttttcat gccctgccct agcgtccaag cctcacggcc gcgctcggcc
    11521 tctctggcgg ccttctggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
    11581 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
    11641 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
    11701 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    11761 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    11821 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    11881 tccgcctttc tcccttcggg aagcgtggcg cttttccgct gcataaccct gcttcggggt
    11941 cattatagcg attttttcgg tatatccatc ctttttcgca cgatatacag gattttgcca
    12001 aagggttcgt gtagactttc cttggtgtat ccaacggcgt cagccgggca ggataggtga
    12061 agtaggccca cccgcgagcg ggtgttcctt cttcactgtc ccttattcgc acctggcggt
    12121 gctcaacggg aatcctgctc tgcgaggctg gccggctacc gccggcgtaa cagatgaggg
    12181 caagcggatg gctgatgaaa ccaagccaac caggaagggc agcccaccta tcaaggtgta
    12241 ctgccttcca gacgaacgaa gagcgattga ggaaaaggcg gcggcggccg gcatgagcct
    12301 gtcggcctac ctgctggccg tcggccaggg ctacaaaatc acgggcgtcg tggactatga
    12361 gcacgtccgc gagctggccc gcatcaatgg cgacctgggc cgcctgggcg gcctgctgaa
    12421 actctggctc accgacgacc cgcgcacggc gcggttcggt gatgccacga tcctcgccct
    12481 gctggcgaag atcgaagaga agcaggacga gcttggcaag gtcatgatgg gcgtggtccg
    12541 cccgagggca gagccatgac ttttttagcc gctaaaacgg ccggggggtg cgcgtgattg
    12601 ccaagcacgt ccccatgcgc tccatcaaga agagcgactt cgcggagctg gtgaagtaca
    12661 tcaccgacga gcaaggcaag accgagcgcc tttgcgacgc tca
//
