LOCUS       V013368                 5361 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013368
VERSION     V013368
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5361)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5361
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        512..857
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             878..1585
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     CDS             1592..2452
                     /gene="PIP2-1"
                     /label="Aquaporin PIP2-1"
                     /note="Aquaporin PIP2-1 from Arabidopsis thaliana.
                     Accession#: P43286"
     terminator      2472..2724
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2733..2749)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        3223..3327
                     /label="AmpR promoter"
     CDS             3328..4185
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4359..4947
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    5235..5256
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5271..5301
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    5309..5325
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5333..5349
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
       61 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      121 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      181 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      241 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      301 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      361 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      421 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      481 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      541 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      601 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      661 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
      721 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
      781 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
      841 ttcatttgga gagaacacgg gggactctag aggatccatg gtgagcaagg gcgaggagga
      901 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa
      961 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     1021 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc
     1081 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt
     1141 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     1201 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa
     1261 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg
     1321 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca
     1381 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc
     1441 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc
     1501 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac
     1561 cggcggcatg gacgagctgt acaagctcga gatggcaaag gatgtggaag ccgttcccgg
     1621 agaaggattt cagacaagag actatcaaga tccgccacca gctccgttta ttgatggagc
     1681 ggagctaaag aagtggtctt tctacagagc agttatcgca gagttcgtag ccactctcct
     1741 cttcttatac atcaccgttt tgacagtcat cggttacaag attcagtccg atactgatgc
     1801 cggtggcgta gattgcggcg gagttggaat cctcggtatc gcttgggcct ttggtggtat
     1861 gatcttcatc ctcgtctact gcaccgccgg tatctctggt ggtcacatta acccagcggt
     1921 gacatttggg ctattcttgg cacgtaaagt gtcgttacct agggccctat tgtacataat
     1981 cgctcagtgt ttgggtgcga tttgtggagt tggttttgtc aaagccttcc aaagctctta
     2041 ctacacccgt tacggaggtg gagccaactc tctagccgat ggctacagca cagggaccgg
     2101 tctagccgca gagatcattg gtactttcgt tcttgtctac accgtcttct ctgccactga
     2161 ccccaaacgt agtgccagag actcccacgt tccggtgttg gcgccacttc caatcggatt
     2221 tgccgtgttc atggtacatt tggctaccat tcccattacc ggaaccggaa ttaacccggc
     2281 aaggagtttc ggagctgccg taatctacaa caagagcaag ccatgggatg accactggat
     2341 attttgggtt ggaccattca ttggagctgc gatagctgca ttctaccacc aattcgttct
     2401 gagagcttca ggttctaagt ctcttggatc attcagaagt gctgccaacg tctaagagct
     2461 cgaatttccc cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
     2521 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
     2581 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
     2641 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
     2701 cggtgtcatc tatgttacta gatcgggaat tcactggccg tcgttttaca acgtcgtgac
     2761 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     2821 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     2881 ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     2941 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     3001 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     3061 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     3121 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     3181 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     3241 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     3301 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     3361 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     3421 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     3481 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     3541 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     3601 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     3661 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     3721 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     3781 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     3841 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     3901 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     3961 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     4021 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     4081 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     4141 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     4201 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     4261 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     4321 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     4381 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     4441 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     4501 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     4561 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     4621 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     4681 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     4741 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     4801 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     4861 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     4921 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     4981 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     5041 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     5101 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     5161 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     5221 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     5281 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     5341 agctatgacc atgattacgc c
//
