LOCUS       62056_3970       13620 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13620)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..13620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(148..516)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(549..658)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(1342..1989)
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    complement(2454..2478)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2634..2817
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     rep_origin      complement(2997..3621)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     terminator      4022..4274
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4823..4844
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4859..4889
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        5461..5806
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             5838..6545
                     /codon_start=1
                     /label=mOrange2
                     /note="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             6552..6569
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     misc_feature    complement(7484..7508)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(9107..10252)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(10554..11345)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  11537..12304
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      complement(13005..13615)
                     /direction=LEFT
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
ORIGIN
        1 tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
     2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
     3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
     3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
     3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
     4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
     4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
     4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
     4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
     4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
     4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
     4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
     4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
     4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4921 caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc cccagattag
     4981 ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt acgcagcagg
     5041 tctcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc ttcccaagaa
     5101 ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat
     5161 atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc ttcacaaacc
     5221 aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat caaaggccat
     5281 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt
     5341 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca
     5401 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
     5461 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
     5521 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
     5581 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
     5641 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
     5701 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
     5761 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactctaga
     5821 ggatccccgg gggtaccatg gtgagcaagg gcgaggagaa taacatggcc atcatcaagg
     5881 agttcatgcg cttcaaggtg cgcatggagg gctccgtgaa cggccacgag ttcgagatcg
     5941 agggcgaggg cgagggccgc ccctacgagg gctttcagac cgctaagctg aaggtgacca
     6001 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcatttcacc tacggctcca
     6061 aggcctacgt gaagcacccc gccgacatcc ccgactactt caagctgtcc ttccccgagg
     6121 gcttcaagtg ggagcgcgtg atgaactacg aggacggcgg cgtggtgacc gtgacccagg
     6181 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
     6241 cctccgacgg ccccgtgatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
     6301 tgtaccccga ggacggtgcc ctgaagggca agatcaagat gaggctgaag ctgaaggacg
     6361 gcggccacta cacctccgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
     6421 ccggcgccta catcgtcgac atcaagttgg acatcacctc ccacaacgag gactacacca
     6481 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     6541 acaagctcga gcaccaccac caccaccact gagagctcga atttccccga tcgttcaaac
     6601 atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata
     6661 taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt
     6721 atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac
     6781 aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat
     6841 cgggaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     6901 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     6961 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gcccgctcct ttcgctttct
     7021 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     7081 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg
     7141 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     7201 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     7261 gctattcttt tgatttataa gggattttgc cgatttcgga accaccatca aacaggattt
     7321 tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt
     7381 gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc cagtacatta
     7441 aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca ccacaatata
     7501 tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa tcaccactcg
     7561 atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta aggcggcaga
     7621 ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg ggtttgaaac
     7681 acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg ttgctgcctg
     7741 tgatcaaata tcatctccct cgcagagatc cgaattatca gccttcttat tcatttctcg
     7801 cttaaccgtg acaggctgtc gatcttgaga actatgccga cataatagga aatcgctgga
     7861 taaagccgct gaggaagctg agtggcgcta tttctttaga agtgaacgtt gacgatatca
     7921 actcccctat ccattgctca ccgaatggta caggtcgggg acccgaagtt ccgactgtcg
     7981 gcctgatgca tccccggctg atcgacccca gatctggggc tgagaaagcc cagtaaggaa
     8041 acaactgtag gttcgagtcg cgagatcccc cggaaccaaa ggaagtaggt taaacccgct
     8101 ccgatcaggc cgagccacgc caggccgaga acattggttc ctgtaggcat cgggattggc
     8161 ggatcaaaca ctaaagctac tggaacgagc agaagtcctc cggccgccag ttgccaggcg
     8221 gtaaaggtga gcagaggcac gggaggttgc cacttgcggg tcagcacggt tccgaacgcc
     8281 atggaaaccg cccccgccag gcccgctgcg acgccgacag gatctagcgc tgcgtttggt
     8341 gtcaacacca acagcgccac gcccgcagtt ccgcaaatag cccccaggac cgccatcaat
     8401 cgtatcgggc tacctagcag agcggcagag atgaacacga ccatcagcgg ctgcacagcg
     8461 cctaccgtcg ccgcgacccc gcccggcagg cggtagaccg aaataaacaa caagctccag
     8521 aatagcgaaa tattaagtgc gccgaggatg aagatgcgca tccaccagat tcccgttgga
     8581 atctgtcgga cgatcatcac gagcaataaa cccgccggca acgcccgcag cagcataccg
     8641 gcgacccctc ggcctcgctg ttcgggctcc acgaaaacgc cggacagatg cgccttgtga
     8701 gcgtccttgg ggccgtcctc ctgtttgaag accgacagcc caatgatctc gccgtcgatg
     8761 taggcgccga atgccacggc atctcgcaac cgttcagcga acgcctccat gggctttttc
     8821 tcctcgtgct cgtaaacgga cccgaacatc tctggagctt tcttcagggc cgacaatcgg
     8881 atctcgcgga aatcctgcac gtcggccgct ccaagccgtc gaatctgagc cttaatcaca
     8941 attgtcaatt ttaatcctct gtttatcggc agttcgtaga gcgcgccgtg cgtcccgagc
     9001 gatactgagc gaagcaagtg cgtcgagcag tgcccgcttg ttcctgaaat gccagtaaag
     9061 cgctggctgc tgaaccccca gccggaactg accccacaag gccctagcgt ttgcaatgca
     9121 ccaggtcatc attgacccag gcgtgttcca ccaggccgct gcctcgcaac tcttcgcagg
     9181 cttcgccgac ctgctcgcgc cacttcttca cgcgggtgga atccgatccg cacatgaggc
     9241 ggaaggtttc cagcttgagc gggtacggct cccggtgcga gctgaaatag tcgaacatcc
     9301 gtcgggccgt cggcgacagc ttgcggtact tctcccatat gaatttcgtg tagtggtcgc
     9361 cagcaaacag cacgacgatt tcctcgtcga tcaggacctg gcaacgggac gttttcttgc
     9421 cacggtccag gacgcggaag cggtgcagca gcgacaccga ttccaggtgc ccaacgcggt
     9481 cggacgtgaa gcccatcgcc gtcgcctgta ggcgcgacag gcattcctcg gccttcgtgt
     9541 aataccggcc attgatcgac cagcccaggt cctggcaaag ctcgtagaac gtgaaggtga
     9601 tcggctcgcc gataggggtg cgcttcgcgt actccaacac ctgctgccac accagttcgt
     9661 catcgtcggc ccgcagctcg acgccggtgt aggtgatctt cacgtccttg ttgacgtgga
     9721 aaatgacctt gttttgcagc gcctcgcgcg ggattttctt gttgcgcgtg gtgaacaggg
     9781 cagagcgggc cgtgtcgttt ggcatcgctc gcatcgtgtc cggccacggc gcaatatcga
     9841 acaaggaaag ctgcatttcc ttgatctgct gcttcgtgtg tttcagcaac gcggcctgct
     9901 tggcctcgct gacctgtttt gccaggtcct cgccggcggt ttttcgcttc ttggtcgtca
     9961 tagttcctcg cgtgtcgatg gtcatcgact tcgccaaacc tgccgcctcc tgttcgagac
    10021 gacgcgaacg ctccacggcg gccgatggcg cgggcagggc agggggagcc agttgcacgc
    10081 tgtcgcgctc gatcttggcc gtagcttgct ggaccatcga gccgacggac tggaaggttt
    10141 cgcggggcgc acgcatgacg gtgcggcttg cgatggtttc ggcatcctcg gcggaaaacc
    10201 ccgcgtcgat cagttcttgc ctgtatgcct tccggtcaaa cgtccgattc attcaccctc
    10261 cttgcgggat tgccccgact cacgccgggg caatgtgccc ttattcctga tttgacccgc
    10321 ctggtgcctt ggtgtccaga taatccacct tatcggcaat gaagtcggtc ccgtagaccg
    10381 tctggccgtc cttctcgtac ttggtattcc gaatcttgcc ctgcacgaat accagcgacc
    10441 ccttgcccaa atacttgccg tgggcctcgg cctgagagcc aaaacacttg atgcggaaga
    10501 agtcggtgcg ctcctgcttg tcgccggcat cgttgcgcca catctaggta ctaaaacaat
    10561 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
    10621 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
    10681 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
    10741 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
    10801 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
    10861 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
    10921 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
    10981 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
    11041 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
    11101 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
    11161 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
    11221 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
    11281 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
    11341 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
    11401 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
    11461 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
    11521 tgaaaccaca attatgggtg atgctgccaa cttactgatt tagtgtatga tggtgttttt
    11581 gaggtgctcc agtggcttct gtgtctatca gctgtccctc ctgttcagct actgacgggg
    11641 tggtgcgtaa cggcaaaagc accgccggac atcagcgcta tctctgctct cactgccgta
    11701 aaacatggca actgcagttc acttacaccg cttctcaacc cggtacgcac cagaaaatca
    11761 ttgatatggc catgaatggc gttggatgcc gggcaacagc ccgcattatg ggcgttggcc
    11821 tcaacacgat tttacgtcac ttaaaaaact caggccgcag tcggtaacct cgcgcataca
    11881 gccgggcagt gacgtcatcg tctgcgcgga aatggacgaa cagtggggct atgtcggggc
    11941 taaatcgcgc cagcgctggc tgttttacgc gtatgacagt ctccggaaga cggttgttgc
    12001 gcacgtattc ggtgaacgca ctatggcgac gctggggcgt cttatgagcc tgctgtcacc
    12061 ctttgacgtg gtgatatgga tgacggatgg ctggccgctg tatgaatccc gcctgaaggg
    12121 aaagctgcac gtaatcagca agcgatatac gcagcgaatt gagcggcata acctgaatct
    12181 gaggcagcac ctggcacggc tgggacggaa gtcgctgtcg ttctcaaaat cggtggagct
    12241 gcatgacaaa gtcatcgggc attatctgaa cataaaacac tatcaataag ttggagtcat
    12301 tacccaatta tgatagaatt tacaagctat aaggttattg tcctgggttt caagcattag
    12361 tccatgcaag tttttatgct ttgcccattc tatagatata ttgataagcg cgctgcctat
    12421 gccttgcccc ctgaaatcct tacatacggc gatatcttct atataaaaga tatattatct
    12481 tatcagtatt gtcaatatat tcaaggcaat ctgcctcctc atcctcttca tcctcttcgt
    12541 cttggtagct ttttaaatat ggcgcttcat agagtaattc tgtaaaggtc caattctcgt
    12601 tttcatacct cggtataatc ttacctatca cctcaaatgg ttcgctgggt ttatcgcacc
    12661 cccgaacacg agcacggcac ccgcgaccac tatgccaaga atgcccaagg taaaaattgc
    12721 cggccccgcc atgaagtccg tgaatgcccc gacggccgaa gtgaagggca ggccgccacc
    12781 caggccgccg ccctcactgc ccggcacctg gtcgctgaat gtcgatgcca gcacctgcgg
    12841 cacgtcaatg cttccgggcg tcgcgctcgg gctgatcgcc catcccgtta ctgccccgat
    12901 cccggcaatg gcaaggactg ccagcgctgc catttttggg gtgaggccgt tcgcggccga
    12961 ggggcgcagc ccctgggggg atgggaggcc cgcgttagcg ggccgggagg gttcgagaag
    13021 ggggggcacc ccccttcggc gtgcgcggtc acgcgcacag ggcgcagccc tggttaaaaa
    13081 caaggtttat aaatattggt ttaaaagcag gttaaaagac aggttagcgg tggccgaaaa
    13141 acgggcggaa acccttgcaa atgctggatt ttctgcctgt ggacagcccc tcaaatgtca
    13201 ataggtgcgc ccctcatctg tcagcactct gcccctcaag tgtcaaggat cgcgcccctc
    13261 atctgtcagt agtcgcgccc ctcaagtgtc aataccgcag ggcacttatc cccaggcttg
    13321 tccacatcat ctgtgggaaa ctcgcgtaaa atcaggcgtt ttcgccgatt tgcgaggctg
    13381 gccagctcca cgtcgccggc cgaaatcgag cctgcccctc atctgtcaac gccgcgccgg
    13441 gtgagtcggc ccctcaagtg tcaacgtccg cccctcatct gtcagtgagg gccaagtttt
    13501 ccgcgaggta tccacaacgc cggcggccgc ggtgtctcgc acacggcttc gacggcgttt
    13561 ctggcgcgtt tgcagggcca tagacggccg ccagcccagc ggcgagggca accagcccgg
//
