LOCUS       62056_3985       14350 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14350)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..14350
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(148..516)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(549..658)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(1342..1989)
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    complement(2454..2478)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2634..2817
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2841..3629
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     rep_origin      complement(3727..4351)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     protein_bind    4823..4844
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4859..4889
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        5461..5806
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             6559..7275
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             7282..7299
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     misc_feature    complement(8214..8238)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(9837..10982)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(11284..12075)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  12267..13034
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      complement(13735..14345)
                     /direction=LEFT
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
ORIGIN
        1 tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
     2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
     3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
     3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
     3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
     4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
     4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
     4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
     4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
     4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
     4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
     4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
     4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
     4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4921 caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc cccagattag
     4981 ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt acgcagcagg
     5041 tctcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc ttcccaagaa
     5101 ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat
     5161 atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc ttcacaaacc
     5221 aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat caaaggccat
     5281 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt
     5341 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca
     5401 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
     5461 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
     5521 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
     5581 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
     5641 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
     5701 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
     5761 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactctaga
     5821 ggatccgcca ccatgaagtt ggctcttatt gcttctattt tggctgctgg ttgtattgct
     5881 ggtcctcttg ctcaaactga atctgaatcc gttgatatgc ctgaacatac cattaactac
     5941 attgatattg ctcctgagga atttgagcca cttaaggcta atctttcttc tcttgtttct
     6001 agggatactt tcccagtgtc tacttgtcca gctggacaaa cttacgatag gtcagtttgt
     6061 tataaggcta atactattag gtctttctgt gttgctaatc ctagatctaa tagggagaaa
     6121 attacagata ccccatgtaa gccacaagaa atttgtgttc aaagaagact tagtaacgga
     6181 aagagtttcg caaagtgtat tccaattgtt aatcttgttg catggaagac atctccagat
     6241 ggaaataagg aaggatgcac aacaacatct gctaatccag ctggatatca tcatcttgga
     6301 acaattgttt acgatgttaa caagaatcca atcgctgtgg ataagatctc ttactttgga
     6361 gaaccaggaa atgttaatga aggaattgga ggatctactt cttacttctc ttctgatctt
     6421 tttcagtttt ctaagtccag atttatgaag acttgtattt tctctggagg atatggaaat
     6481 cttaatgctt atacttgggc ttgggagggc ggcggaggga gtggaggagg gggaagcgga
     6541 ggcggaggga gtggtaccat ggtgtccaag ggagaggagt tgtttactgg agttgttcca
     6601 attcttgttg aattggatgg agatgttaat ggacataagt tttctgtttc tggagaggga
     6661 gaaggagatg ctacttatgg taagttgact ttgaagttta tctgtactac tggtaagttg
     6721 ccagttcctt ggccaacttt ggttactact cttacttatg gtgttcaatg tttttctagg
     6781 tatccagatc acatgaagca gcatgatttt tttaagtctg ctatgccaga gggatatgtt
     6841 caagaaagaa ctattttttt caaggatgat ggaaactaca agactagggc tgaagtgaag
     6901 ttcgaaggag atacacttgt taataggatt gaattgaagg gaattgattt caaggaggat
     6961 ggaaacattt tgggtcataa gttggaatat aactacaact cacataacgt ttacatcatg
     7021 gctgataagc aaaagaatgg aattaaggtg aatttcaaga tcagacataa cattgaagat
     7081 ggatctgttc aacttgctga tcattatcaa caaaatactc caatcggaga tggaccagtg
     7141 ttgttgccag ataaccatta cttgagtacc caatcagctc tttctaaaga tccaaatgag
     7201 aagagggatc acatggttct tcttgaattt gtgacagctg ctggaattac acttggaatg
     7261 gatgaacttt acaagctcga gcaccaccac caccaccact gagagctcga atttccccga
     7321 tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat
     7381 gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat
     7441 gacgttattt atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc
     7501 gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat
     7561 gttactagat cgggaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     7621 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     7681 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gcccgctcct
     7741 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     7801 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     7861 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     7921 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     7981 cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcgga accaccatca
     8041 aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg
     8101 gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc
     8161 cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca
     8221 ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa
     8281 tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta
     8341 aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg
     8401 ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg
     8461 ttgctgcctg tgatcaaata tcatctccct cgcagagatc cgaattatca gccttcttat
     8521 tcatttctcg cttaaccgtg acaggctgtc gatcttgaga actatgccga cataatagga
     8581 aatcgctgga taaagccgct gaggaagctg agtggcgcta tttctttaga agtgaacgtt
     8641 gacgatatca actcccctat ccattgctca ccgaatggta caggtcgggg acccgaagtt
     8701 ccgactgtcg gcctgatgca tccccggctg atcgacccca gatctggggc tgagaaagcc
     8761 cagtaaggaa acaactgtag gttcgagtcg cgagatcccc cggaaccaaa ggaagtaggt
     8821 taaacccgct ccgatcaggc cgagccacgc caggccgaga acattggttc ctgtaggcat
     8881 cgggattggc ggatcaaaca ctaaagctac tggaacgagc agaagtcctc cggccgccag
     8941 ttgccaggcg gtaaaggtga gcagaggcac gggaggttgc cacttgcggg tcagcacggt
     9001 tccgaacgcc atggaaaccg cccccgccag gcccgctgcg acgccgacag gatctagcgc
     9061 tgcgtttggt gtcaacacca acagcgccac gcccgcagtt ccgcaaatag cccccaggac
     9121 cgccatcaat cgtatcgggc tacctagcag agcggcagag atgaacacga ccatcagcgg
     9181 ctgcacagcg cctaccgtcg ccgcgacccc gcccggcagg cggtagaccg aaataaacaa
     9241 caagctccag aatagcgaaa tattaagtgc gccgaggatg aagatgcgca tccaccagat
     9301 tcccgttgga atctgtcgga cgatcatcac gagcaataaa cccgccggca acgcccgcag
     9361 cagcataccg gcgacccctc ggcctcgctg ttcgggctcc acgaaaacgc cggacagatg
     9421 cgccttgtga gcgtccttgg ggccgtcctc ctgtttgaag accgacagcc caatgatctc
     9481 gccgtcgatg taggcgccga atgccacggc atctcgcaac cgttcagcga acgcctccat
     9541 gggctttttc tcctcgtgct cgtaaacgga cccgaacatc tctggagctt tcttcagggc
     9601 cgacaatcgg atctcgcgga aatcctgcac gtcggccgct ccaagccgtc gaatctgagc
     9661 cttaatcaca attgtcaatt ttaatcctct gtttatcggc agttcgtaga gcgcgccgtg
     9721 cgtcccgagc gatactgagc gaagcaagtg cgtcgagcag tgcccgcttg ttcctgaaat
     9781 gccagtaaag cgctggctgc tgaaccccca gccggaactg accccacaag gccctagcgt
     9841 ttgcaatgca ccaggtcatc attgacccag gcgtgttcca ccaggccgct gcctcgcaac
     9901 tcttcgcagg cttcgccgac ctgctcgcgc cacttcttca cgcgggtgga atccgatccg
     9961 cacatgaggc ggaaggtttc cagcttgagc gggtacggct cccggtgcga gctgaaatag
    10021 tcgaacatcc gtcgggccgt cggcgacagc ttgcggtact tctcccatat gaatttcgtg
    10081 tagtggtcgc cagcaaacag cacgacgatt tcctcgtcga tcaggacctg gcaacgggac
    10141 gttttcttgc cacggtccag gacgcggaag cggtgcagca gcgacaccga ttccaggtgc
    10201 ccaacgcggt cggacgtgaa gcccatcgcc gtcgcctgta ggcgcgacag gcattcctcg
    10261 gccttcgtgt aataccggcc attgatcgac cagcccaggt cctggcaaag ctcgtagaac
    10321 gtgaaggtga tcggctcgcc gataggggtg cgcttcgcgt actccaacac ctgctgccac
    10381 accagttcgt catcgtcggc ccgcagctcg acgccggtgt aggtgatctt cacgtccttg
    10441 ttgacgtgga aaatgacctt gttttgcagc gcctcgcgcg ggattttctt gttgcgcgtg
    10501 gtgaacaggg cagagcgggc cgtgtcgttt ggcatcgctc gcatcgtgtc cggccacggc
    10561 gcaatatcga acaaggaaag ctgcatttcc ttgatctgct gcttcgtgtg tttcagcaac
    10621 gcggcctgct tggcctcgct gacctgtttt gccaggtcct cgccggcggt ttttcgcttc
    10681 ttggtcgtca tagttcctcg cgtgtcgatg gtcatcgact tcgccaaacc tgccgcctcc
    10741 tgttcgagac gacgcgaacg ctccacggcg gccgatggcg cgggcagggc agggggagcc
    10801 agttgcacgc tgtcgcgctc gatcttggcc gtagcttgct ggaccatcga gccgacggac
    10861 tggaaggttt cgcggggcgc acgcatgacg gtgcggcttg cgatggtttc ggcatcctcg
    10921 gcggaaaacc ccgcgtcgat cagttcttgc ctgtatgcct tccggtcaaa cgtccgattc
    10981 attcaccctc cttgcgggat tgccccgact cacgccgggg caatgtgccc ttattcctga
    11041 tttgacccgc ctggtgcctt ggtgtccaga taatccacct tatcggcaat gaagtcggtc
    11101 ccgtagaccg tctggccgtc cttctcgtac ttggtattcc gaatcttgcc ctgcacgaat
    11161 accagcgacc ccttgcccaa atacttgccg tgggcctcgg cctgagagcc aaaacacttg
    11221 atgcggaaga agtcggtgcg ctcctgcttg tcgccggcat cgttgcgcca catctaggta
    11281 ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc
    11341 cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg
    11401 gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa
    11461 gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa
    11521 gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt
    11581 aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa
    11641 gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc
    11701 gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg
    11761 ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct
    11821 ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca
    11881 tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt
    11941 catttttaaa tataggtttt cattttctcc caccagctta tataccttag caggagacat
    12001 tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat
    12061 tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa
    12121 gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa
    12181 taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg
    12241 gagccgattt tgaaaccaca attatgggtg atgctgccaa cttactgatt tagtgtatga
    12301 tggtgttttt gaggtgctcc agtggcttct gtgtctatca gctgtccctc ctgttcagct
    12361 actgacgggg tggtgcgtaa cggcaaaagc accgccggac atcagcgcta tctctgctct
    12421 cactgccgta aaacatggca actgcagttc acttacaccg cttctcaacc cggtacgcac
    12481 cagaaaatca ttgatatggc catgaatggc gttggatgcc gggcaacagc ccgcattatg
    12541 ggcgttggcc tcaacacgat tttacgtcac ttaaaaaact caggccgcag tcggtaacct
    12601 cgcgcataca gccgggcagt gacgtcatcg tctgcgcgga aatggacgaa cagtggggct
    12661 atgtcggggc taaatcgcgc cagcgctggc tgttttacgc gtatgacagt ctccggaaga
    12721 cggttgttgc gcacgtattc ggtgaacgca ctatggcgac gctggggcgt cttatgagcc
    12781 tgctgtcacc ctttgacgtg gtgatatgga tgacggatgg ctggccgctg tatgaatccc
    12841 gcctgaaggg aaagctgcac gtaatcagca agcgatatac gcagcgaatt gagcggcata
    12901 acctgaatct gaggcagcac ctggcacggc tgggacggaa gtcgctgtcg ttctcaaaat
    12961 cggtggagct gcatgacaaa gtcatcgggc attatctgaa cataaaacac tatcaataag
    13021 ttggagtcat tacccaatta tgatagaatt tacaagctat aaggttattg tcctgggttt
    13081 caagcattag tccatgcaag tttttatgct ttgcccattc tatagatata ttgataagcg
    13141 cgctgcctat gccttgcccc ctgaaatcct tacatacggc gatatcttct atataaaaga
    13201 tatattatct tatcagtatt gtcaatatat tcaaggcaat ctgcctcctc atcctcttca
    13261 tcctcttcgt cttggtagct ttttaaatat ggcgcttcat agagtaattc tgtaaaggtc
    13321 caattctcgt tttcatacct cggtataatc ttacctatca cctcaaatgg ttcgctgggt
    13381 ttatcgcacc cccgaacacg agcacggcac ccgcgaccac tatgccaaga atgcccaagg
    13441 taaaaattgc cggccccgcc atgaagtccg tgaatgcccc gacggccgaa gtgaagggca
    13501 ggccgccacc caggccgccg ccctcactgc ccggcacctg gtcgctgaat gtcgatgcca
    13561 gcacctgcgg cacgtcaatg cttccgggcg tcgcgctcgg gctgatcgcc catcccgtta
    13621 ctgccccgat cccggcaatg gcaaggactg ccagcgctgc catttttggg gtgaggccgt
    13681 tcgcggccga ggggcgcagc ccctgggggg atgggaggcc cgcgttagcg ggccgggagg
    13741 gttcgagaag ggggggcacc ccccttcggc gtgcgcggtc acgcgcacag ggcgcagccc
    13801 tggttaaaaa caaggtttat aaatattggt ttaaaagcag gttaaaagac aggttagcgg
    13861 tggccgaaaa acgggcggaa acccttgcaa atgctggatt ttctgcctgt ggacagcccc
    13921 tcaaatgtca ataggtgcgc ccctcatctg tcagcactct gcccctcaag tgtcaaggat
    13981 cgcgcccctc atctgtcagt agtcgcgccc ctcaagtgtc aataccgcag ggcacttatc
    14041 cccaggcttg tccacatcat ctgtgggaaa ctcgcgtaaa atcaggcgtt ttcgccgatt
    14101 tgcgaggctg gccagctcca cgtcgccggc cgaaatcgag cctgcccctc atctgtcaac
    14161 gccgcgccgg gtgagtcggc ccctcaagtg tcaacgtccg cccctcatct gtcagtgagg
    14221 gccaagtttt ccgcgaggta tccacaacgc cggcggccgc ggtgtctcgc acacggcttc
    14281 gacggcgttt ctggcgcgtt tgcagggcca tagacggccg ccagcccagc ggcgagggca
    14341 accagcccgg
//
