LOCUS       62056_3965       13573 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13573)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..13573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(148..516)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(549..658)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(1342..1989)
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    complement(2454..2478)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2634..2817
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     rep_origin      complement(2950..3575)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     terminator      4022..4274
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4823..4844
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4859..4889
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        5461..5806
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             5848..6522
                     /codon_start=1
                     /label=mRFP1
                     /note="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
                     /translation="MAFSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     misc_feature    complement(7437..7461)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(9060..10205)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(10507..11298)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  11490..12257
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      complement(12958..13568)
                     /direction=LEFT
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
ORIGIN
        1 tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
     2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
     3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
     3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
     3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
     4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
     4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
     4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
     4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
     4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
     4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
     4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
     4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
     4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4921 caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc cccagattag
     4981 ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt acgcagcagg
     5041 tctcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc ttcccaagaa
     5101 ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat
     5161 atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc ttcacaaacc
     5221 aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat caaaggccat
     5281 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt
     5341 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca
     5401 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
     5461 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
     5521 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
     5581 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
     5641 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
     5701 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
     5761 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactctaga
     5821 ggatccccgg gggtacctag actcgagatg gccttctccg aggacgtcat caaggagttc
     5881 atgcgcttca aggtgcgcat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     5941 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc
     6001 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tccagtacgg ctccaaggcc
     6061 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     6121 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     6181 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     6241 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga gcggatgtac
     6301 cccgaggacg gcgccctgaa gggcgagatc aagatgaggc tgaagctgaa ggacggcggc
     6361 cactacgacg ccgaggtcaa gaccacctac atggccaaga agcccgtgca gctgcccggc
     6421 gcctacaaga ccgacatcaa gctggacatc acctcccaca acgaggacta caccatcgtg
     6481 gaacagtacg agcgcgccga gggccgccac tccaccggcg cctgagagct cgaatttccc
     6541 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     6601 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     6661 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     6721 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     6781 tatgttacta gatcgggaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     6841 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     6901 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgcccgct
     6961 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     7021 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     7081 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     7141 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     7201 aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc ggaaccacca
     7261 tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc
     7321 agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca
     7381 ccccagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt
     7441 acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca
     7501 aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt
     7561 gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg
     7621 ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga
     7681 gcgttgctgc ctgtgatcaa atatcatctc cctcgcagag atccgaatta tcagccttct
     7741 tattcatttc tcgcttaacc gtgacaggct gtcgatcttg agaactatgc cgacataata
     7801 ggaaatcgct ggataaagcc gctgaggaag ctgagtggcg ctatttcttt agaagtgaac
     7861 gttgacgata tcaactcccc tatccattgc tcaccgaatg gtacaggtcg gggacccgaa
     7921 gttccgactg tcggcctgat gcatccccgg ctgatcgacc ccagatctgg ggctgagaaa
     7981 gcccagtaag gaaacaactg taggttcgag tcgcgagatc ccccggaacc aaaggaagta
     8041 ggttaaaccc gctccgatca ggccgagcca cgccaggccg agaacattgg ttcctgtagg
     8101 catcgggatt ggcggatcaa acactaaagc tactggaacg agcagaagtc ctccggccgc
     8161 cagttgccag gcggtaaagg tgagcagagg cacgggaggt tgccacttgc gggtcagcac
     8221 ggttccgaac gccatggaaa ccgcccccgc caggcccgct gcgacgccga caggatctag
     8281 cgctgcgttt ggtgtcaaca ccaacagcgc cacgcccgca gttccgcaaa tagcccccag
     8341 gaccgccatc aatcgtatcg ggctacctag cagagcggca gagatgaaca cgaccatcag
     8401 cggctgcaca gcgcctaccg tcgccgcgac cccgcccggc aggcggtaga ccgaaataaa
     8461 caacaagctc cagaatagcg aaatattaag tgcgccgagg atgaagatgc gcatccacca
     8521 gattcccgtt ggaatctgtc ggacgatcat cacgagcaat aaacccgccg gcaacgcccg
     8581 cagcagcata ccggcgaccc ctcggcctcg ctgttcgggc tccacgaaaa cgccggacag
     8641 atgcgccttg tgagcgtcct tggggccgtc ctcctgtttg aagaccgaca gcccaatgat
     8701 ctcgccgtcg atgtaggcgc cgaatgccac ggcatctcgc aaccgttcag cgaacgcctc
     8761 catgggcttt ttctcctcgt gctcgtaaac ggacccgaac atctctggag ctttcttcag
     8821 ggccgacaat cggatctcgc ggaaatcctg cacgtcggcc gctccaagcc gtcgaatctg
     8881 agccttaatc acaattgtca attttaatcc tctgtttatc ggcagttcgt agagcgcgcc
     8941 gtgcgtcccg agcgatactg agcgaagcaa gtgcgtcgag cagtgcccgc ttgttcctga
     9001 aatgccagta aagcgctggc tgctgaaccc ccagccggaa ctgaccccac aaggccctag
     9061 cgtttgcaat gcaccaggtc atcattgacc caggcgtgtt ccaccaggcc gctgcctcgc
     9121 aactcttcgc aggcttcgcc gacctgctcg cgccacttct tcacgcgggt ggaatccgat
     9181 ccgcacatga ggcggaaggt ttccagcttg agcgggtacg gctcccggtg cgagctgaaa
     9241 tagtcgaaca tccgtcgggc cgtcggcgac agcttgcggt acttctccca tatgaatttc
     9301 gtgtagtggt cgccagcaaa cagcacgacg atttcctcgt cgatcaggac ctggcaacgg
     9361 gacgttttct tgccacggtc caggacgcgg aagcggtgca gcagcgacac cgattccagg
     9421 tgcccaacgc ggtcggacgt gaagcccatc gccgtcgcct gtaggcgcga caggcattcc
     9481 tcggccttcg tgtaataccg gccattgatc gaccagccca ggtcctggca aagctcgtag
     9541 aacgtgaagg tgatcggctc gccgataggg gtgcgcttcg cgtactccaa cacctgctgc
     9601 cacaccagtt cgtcatcgtc ggcccgcagc tcgacgccgg tgtaggtgat cttcacgtcc
     9661 ttgttgacgt ggaaaatgac cttgttttgc agcgcctcgc gcgggatttt cttgttgcgc
     9721 gtggtgaaca gggcagagcg ggccgtgtcg tttggcatcg ctcgcatcgt gtccggccac
     9781 ggcgcaatat cgaacaagga aagctgcatt tccttgatct gctgcttcgt gtgtttcagc
     9841 aacgcggcct gcttggcctc gctgacctgt tttgccaggt cctcgccggc ggtttttcgc
     9901 ttcttggtcg tcatagttcc tcgcgtgtcg atggtcatcg acttcgccaa acctgccgcc
     9961 tcctgttcga gacgacgcga acgctccacg gcggccgatg gcgcgggcag ggcaggggga
    10021 gccagttgca cgctgtcgcg ctcgatcttg gccgtagctt gctggaccat cgagccgacg
    10081 gactggaagg tttcgcgggg cgcacgcatg acggtgcggc ttgcgatggt ttcggcatcc
    10141 tcggcggaaa accccgcgtc gatcagttct tgcctgtatg ccttccggtc aaacgtccga
    10201 ttcattcacc ctccttgcgg gattgccccg actcacgccg gggcaatgtg cccttattcc
    10261 tgatttgacc cgcctggtgc cttggtgtcc agataatcca ccttatcggc aatgaagtcg
    10321 gtcccgtaga ccgtctggcc gtccttctcg tacttggtat tccgaatctt gccctgcacg
    10381 aataccagcg accccttgcc caaatacttg ccgtgggcct cggcctgaga gccaaaacac
    10441 ttgatgcgga agaagtcggt gcgctcctgc ttgtcgccgg catcgttgcg ccacatctag
    10501 gtactaaaac aattcatcca gtaaaatata atattttatt ttctcccaat caggcttgat
    10561 ccccagtaag tcaaaaaata gctcgacata ctgttcttcc ccgatatcct ccctgatcga
    10621 ccggacgcag aaggcaatgt cataccactt gtccgccctg ccgcttctcc caagatcaat
    10681 aaagccactt actttgccat ctttcacaaa gatgttgctg tctcccaggt cgccgtggga
    10741 aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc
    10801 tttaaatgga gtgtcttctt cccagttttc gcaatccaca tcggccagat cgttattcag
    10861 taagtaatcc aattcggcta agcggctgtc taagctattc gtatagggac aatccgatat
    10921 gtcgatggag tgaaagagcc tgatgcactc cgcatacagc tcgataatct tttcagggct
    10981 ttgttcatct tcatactctt ccgagcaaag gacgccatcg gcctcactca tgagcagatt
    11041 gctccagcca tcatgccgtt caaagtgcag gacctttgga acaggcagct ttccttccag
    11101 ccatagcatc atgtcctttt cccgttccac atcataggtg gtccctttat accggctgtc
    11161 cgtcattttt aaatataggt tttcattttc tcccaccagc ttatatacct tagcaggaga
    11221 cattccttcc gtatctttta cgcagcggta tttttcgatc agttttttca attccggtga
    11281 tattctcatt ttagccattt attatttcct tcctcttttc tacagtattt aaagataccc
    11341 caagaagcta attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt
    11401 aaataccaga aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg
    11461 acggagccga ttttgaaacc acaattatgg gtgatgctgc caacttactg atttagtgta
    11521 tgatggtgtt tttgaggtgc tccagtggct tctgtgtcta tcagctgtcc ctcctgttca
    11581 gctactgacg gggtggtgcg taacggcaaa agcaccgccg gacatcagcg ctatctctgc
    11641 tctcactgcc gtaaaacatg gcaactgcag ttcacttaca ccgcttctca acccggtacg
    11701 caccagaaaa tcattgatat ggccatgaat ggcgttggat gccgggcaac agcccgcatt
    11761 atgggcgttg gcctcaacac gattttacgt cacttaaaaa actcaggccg cagtcggtaa
    11821 cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc ggaaatggac gaacagtggg
    11881 gctatgtcgg ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac agtctccgga
    11941 agacggttgt tgcgcacgta ttcggtgaac gcactatggc gacgctgggg cgtcttatga
    12001 gcctgctgtc accctttgac gtggtgatat ggatgacgga tggctggccg ctgtatgaat
    12061 cccgcctgaa gggaaagctg cacgtaatca gcaagcgata tacgcagcga attgagcggc
    12121 ataacctgaa tctgaggcag cacctggcac ggctgggacg gaagtcgctg tcgttctcaa
    12181 aatcggtgga gctgcatgac aaagtcatcg ggcattatct gaacataaaa cactatcaat
    12241 aagttggagt cattacccaa ttatgataga atttacaagc tataaggtta ttgtcctggg
    12301 tttcaagcat tagtccatgc aagtttttat gctttgccca ttctatagat atattgataa
    12361 gcgcgctgcc tatgccttgc cccctgaaat ccttacatac ggcgatatct tctatataaa
    12421 agatatatta tcttatcagt attgtcaata tattcaaggc aatctgcctc ctcatcctct
    12481 tcatcctctt cgtcttggta gctttttaaa tatggcgctt catagagtaa ttctgtaaag
    12541 gtccaattct cgttttcata cctcggtata atcttaccta tcacctcaaa tggttcgctg
    12601 ggtttatcgc acccccgaac acgagcacgg cacccgcgac cactatgcca agaatgccca
    12661 aggtaaaaat tgccggcccc gccatgaagt ccgtgaatgc cccgacggcc gaagtgaagg
    12721 gcaggccgcc acccaggccg ccgccctcac tgcccggcac ctggtcgctg aatgtcgatg
    12781 ccagcacctg cggcacgtca atgcttccgg gcgtcgcgct cgggctgatc gcccatcccg
    12841 ttactgcccc gatcccggca atggcaagga ctgccagcgc tgccattttt ggggtgaggc
    12901 cgttcgcggc cgaggggcgc agcccctggg gggatgggag gcccgcgtta gcgggccggg
    12961 agggttcgag aagggggggc accccccttc ggcgtgcgcg gtcacgcgca cagggcgcag
    13021 ccctggttaa aaacaaggtt tataaatatt ggtttaaaag caggttaaaa gacaggttag
    13081 cggtggccga aaaacgggcg gaaacccttg caaatgctgg attttctgcc tgtggacagc
    13141 ccctcaaatg tcaataggtg cgcccctcat ctgtcagcac tctgcccctc aagtgtcaag
    13201 gatcgcgccc ctcatctgtc agtagtcgcg cccctcaagt gtcaataccg cagggcactt
    13261 atccccaggc ttgtccacat catctgtggg aaactcgcgt aaaatcaggc gttttcgccg
    13321 atttgcgagg ctggccagct ccacgtcgcc ggccgaaatc gagcctgccc ctcatctgtc
    13381 aacgccgcgc cgggtgagtc ggcccctcaa gtgtcaacgt ccgcccctca tctgtcagtg
    13441 agggccaagt tttccgcgag gtatccacaa cgccggcggc cgcggtgtct cgcacacggc
    13501 ttcgacggcg tttctggcgc gtttgcaggg ccatagacgg ccgccagccc agcggcgagg
    13561 gcaaccagcc cgg
//
