LOCUS       40924_6387       13612 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13612)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 13612)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(148..516)
                     /label=traJ
                     /note="oriT-recognizing protein"
     oriT            complement(549..658)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(1342..1989)
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
     misc_feature    complement(2454..2478)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2634..2817
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     rep_origin      complement(2989..3614)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     terminator      4022..4274
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4823..4844
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4859..4889
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4897..4913
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4921..4937
                     /label=M13 rev
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5461..5806
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             5845..6561
                     /label=EGFP
                     /note="enhanced GFP"
     terminator      6581..6833
                     /note="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(6842..6858)
                     /label=M13 fwd
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(7476..7500)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(9099..10244)
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     CDS             complement(10546..11337)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     mobile_element  11529..12296
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      complement(12997..13607)
                     /direction=LEFT
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
ORIGIN
        1 tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
     2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
     3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
     3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
     3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
     4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
     4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
     4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
     4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
     4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
     4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
     4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
     4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
     4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4921 caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc cccagattag
     4981 ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt acgcagcagg
     5041 tctcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc ttcccaagaa
     5101 ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat
     5161 atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc ttcacaaacc
     5221 aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat caaaggccat
     5281 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt
     5341 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca
     5401 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
     5461 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
     5521 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
     5581 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
     5641 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
     5701 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
     5761 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactctaga
     5821 ggatccccgg gtggtcagtc ccttatggtg agcaagggcg aggagctgtt caccggggtg
     5881 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     5941 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     6001 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     6061 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     6121 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     6181 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     6241 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     6301 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     6361 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     6421 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     6481 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     6541 ggcatggacg agctgtacaa gtaagagctc gaatttcccc gatcgttcaa acatttggca
     6601 ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct
     6661 gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg
     6721 ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata
     6781 gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt
     6841 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     6901 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     6961 gcccttccca acagttgcgc agcctgaatg gcgcccgctc ctttcgcttt cttcccttcc
     7021 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     7081 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
     7141 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     7201 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc gggctattct
     7261 tttgatttat aagggatttt gccgatttcg gaaccaccat caaacaggat tttcgcctgc
     7321 tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca
     7381 atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cccagtacat taaaaacgtc
     7441 cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca
     7501 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
     7561 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
     7621 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
     7681 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcaaa
     7741 tatcatctcc ctcgcagaga tccgaattat cagccttctt attcatttct cgcttaaccg
     7801 tgacaggctg tcgatcttga gaactatgcc gacataatag gaaatcgctg gataaagccg
     7861 ctgaggaagc tgagtggcgc tatttcttta gaagtgaacg ttgacgatat caactcccct
     7921 atccattgct caccgaatgg tacaggtcgg ggacccgaag ttccgactgt cggcctgatg
     7981 catccccggc tgatcgaccc cagatctggg gctgagaaag cccagtaagg aaacaactgt
     8041 aggttcgagt cgcgagatcc cccggaacca aaggaagtag gttaaacccg ctccgatcag
     8101 gccgagccac gccaggccga gaacattggt tcctgtaggc atcgggattg gcggatcaaa
     8161 cactaaagct actggaacga gcagaagtcc tccggccgcc agttgccagg cggtaaaggt
     8221 gagcagaggc acgggaggtt gccacttgcg ggtcagcacg gttccgaacg ccatggaaac
     8281 cgcccccgcc aggcccgctg cgacgccgac aggatctagc gctgcgtttg gtgtcaacac
     8341 caacagcgcc acgcccgcag ttccgcaaat agcccccagg accgccatca atcgtatcgg
     8401 gctacctagc agagcggcag agatgaacac gaccatcagc ggctgcacag cgcctaccgt
     8461 cgccgcgacc ccgcccggca ggcggtagac cgaaataaac aacaagctcc agaatagcga
     8521 aatattaagt gcgccgagga tgaagatgcg catccaccag attcccgttg gaatctgtcg
     8581 gacgatcatc acgagcaata aacccgccgg caacgcccgc agcagcatac cggcgacccc
     8641 tcggcctcgc tgttcgggct ccacgaaaac gccggacaga tgcgccttgt gagcgtcctt
     8701 ggggccgtcc tcctgtttga agaccgacag cccaatgatc tcgccgtcga tgtaggcgcc
     8761 gaatgccacg gcatctcgca accgttcagc gaacgcctcc atgggctttt tctcctcgtg
     8821 ctcgtaaacg gacccgaaca tctctggagc tttcttcagg gccgacaatc ggatctcgcg
     8881 gaaatcctgc acgtcggccg ctccaagccg tcgaatctga gccttaatca caattgtcaa
     8941 ttttaatcct ctgtttatcg gcagttcgta gagcgcgccg tgcgtcccga gcgatactga
     9001 gcgaagcaag tgcgtcgagc agtgcccgct tgttcctgaa atgccagtaa agcgctggct
     9061 gctgaacccc cagccggaac tgaccccaca aggccctagc gtttgcaatg caccaggtca
     9121 tcattgaccc aggcgtgttc caccaggccg ctgcctcgca actcttcgca ggcttcgccg
     9181 acctgctcgc gccacttctt cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt
     9241 tccagcttga gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc
     9301 gtcggcgaca gcttgcggta cttctcccat atgaatttcg tgtagtggtc gccagcaaac
     9361 agcacgacga tttcctcgtc gatcaggacc tggcaacggg acgttttctt gccacggtcc
     9421 aggacgcgga agcggtgcag cagcgacacc gattccaggt gcccaacgcg gtcggacgtg
     9481 aagcccatcg ccgtcgcctg taggcgcgac aggcattcct cggccttcgt gtaataccgg
     9541 ccattgatcg accagcccag gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg
     9601 ccgatagggg tgcgcttcgc gtactccaac acctgctgcc acaccagttc gtcatcgtcg
     9661 gcccgcagct cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc
     9721 ttgttttgca gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg
     9781 gccgtgtcgt ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa
     9841 agctgcattt ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg
     9901 ctgacctgtt ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt catagttcct
     9961 cgcgtgtcga tggtcatcga cttcgccaaa cctgccgcct cctgttcgag acgacgcgaa
    10021 cgctccacgg cggccgatgg cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc
    10081 tcgatcttgg ccgtagcttg ctggaccatc gagccgacgg actggaaggt ttcgcggggc
    10141 gcacgcatga cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg
    10201 atcagttctt gcctgtatgc cttccggtca aacgtccgat tcattcaccc tccttgcggg
    10261 attgccccga ctcacgccgg ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc
    10321 ttggtgtcca gataatccac cttatcggca atgaagtcgg tcccgtagac cgtctggccg
    10381 tccttctcgt acttggtatt ccgaatcttg ccctgcacga ataccagcga ccccttgccc
    10441 aaatacttgc cgtgggcctc ggcctgagag ccaaaacact tgatgcggaa gaagtcggtg
    10501 cgctcctgct tgtcgccggc atcgttgcgc cacatctagg tactaaaaca attcatccag
    10561 taaaatataa tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag
    10621 ctcgacatac tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc
    10681 ataccacttg tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc
    10741 tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg
    10801 cttttccgtc tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc
    10861 ccagttttcg caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa
    10921 gcggctgtct aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct
    10981 gatgcactcc gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc
    11041 cgagcaaagg acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc
    11101 aaagtgcagg acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc
    11161 ccgttccaca tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt
    11221 ttcattttct cccaccagct tatatacctt agcaggagac attccttccg tatcttttac
    11281 gcagcggtat ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta
    11341 ttatttcctt cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag
    11401 acgaactcca attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt
    11461 tcaaagttgt tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaacca
    11521 caattatggg tgatgctgcc aacttactga tttagtgtat gatggtgttt ttgaggtgct
    11581 ccagtggctt ctgtgtctat cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt
    11641 aacggcaaaa gcaccgccgg acatcagcgc tatctctgct ctcactgccg taaaacatgg
    11701 caactgcagt tcacttacac cgcttctcaa cccggtacgc accagaaaat cattgatatg
    11761 gccatgaatg gcgttggatg ccgggcaaca gcccgcatta tgggcgttgg cctcaacacg
    11821 attttacgtc acttaaaaaa ctcaggccgc agtcggtaac ctcgcgcata cagccgggca
    11881 gtgacgtcat cgtctgcgcg gaaatggacg aacagtgggg ctatgtcggg gctaaatcgc
    11941 gccagcgctg gctgttttac gcgtatgaca gtctccggaa gacggttgtt gcgcacgtat
    12001 tcggtgaacg cactatggcg acgctggggc gtcttatgag cctgctgtca ccctttgacg
    12061 tggtgatatg gatgacggat ggctggccgc tgtatgaatc ccgcctgaag ggaaagctgc
    12121 acgtaatcag caagcgatat acgcagcgaa ttgagcggca taacctgaat ctgaggcagc
    12181 acctggcacg gctgggacgg aagtcgctgt cgttctcaaa atcggtggag ctgcatgaca
    12241 aagtcatcgg gcattatctg aacataaaac actatcaata agttggagtc attacccaat
    12301 tatgatagaa tttacaagct ataaggttat tgtcctgggt ttcaagcatt agtccatgca
    12361 agtttttatg ctttgcccat tctatagata tattgataag cgcgctgcct atgccttgcc
    12421 ccctgaaatc cttacatacg gcgatatctt ctatataaaa gatatattat cttatcagta
    12481 ttgtcaatat attcaaggca atctgcctcc tcatcctctt catcctcttc gtcttggtag
    12541 ctttttaaat atggcgcttc atagagtaat tctgtaaagg tccaattctc gttttcatac
    12601 ctcggtataa tcttacctat cacctcaaat ggttcgctgg gtttatcgca cccccgaaca
    12661 cgagcacggc acccgcgacc actatgccaa gaatgcccaa ggtaaaaatt gccggccccg
    12721 ccatgaagtc cgtgaatgcc ccgacggccg aagtgaaggg caggccgcca cccaggccgc
    12781 cgccctcact gcccggcacc tggtcgctga atgtcgatgc cagcacctgc ggcacgtcaa
    12841 tgcttccggg cgtcgcgctc gggctgatcg cccatcccgt tactgccccg atcccggcaa
    12901 tggcaaggac tgccagcgct gccatttttg gggtgaggcc gttcgcggcc gaggggcgca
    12961 gcccctgggg ggatgggagg cccgcgttag cgggccggga gggttcgaga agggggggca
    13021 ccccccttcg gcgtgcgcgg tcacgcgcac agggcgcagc cctggttaaa aacaaggttt
    13081 ataaatattg gtttaaaagc aggttaaaag acaggttagc ggtggccgaa aaacgggcgg
    13141 aaacccttgc aaatgctgga ttttctgcct gtggacagcc cctcaaatgt caataggtgc
    13201 gcccctcatc tgtcagcact ctgcccctca agtgtcaagg atcgcgcccc tcatctgtca
    13261 gtagtcgcgc ccctcaagtg tcaataccgc agggcactta tccccaggct tgtccacatc
    13321 atctgtggga aactcgcgta aaatcaggcg ttttcgccga tttgcgaggc tggccagctc
    13381 cacgtcgccg gccgaaatcg agcctgcccc tcatctgtca acgccgcgcc gggtgagtcg
    13441 gcccctcaag tgtcaacgtc cgcccctcat ctgtcagtga gggccaagtt ttccgcgagg
    13501 tatccacaac gccggcggcc gcggtgtctc gcacacggct tcgacggcgt ttctggcgcg
    13561 tttgcagggc catagacggc cgccagccca gcggcgaggg caaccagccc gg
//
