LOCUS       62056_3955       14562 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14562)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..14562
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(148..516)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(549..658)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(1342..1989)
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    complement(2454..2478)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2634..2817
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2841..3629
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     rep_origin      complement(3939..4563)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     protein_bind    4823..4844
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4859..4889
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        5461..5806
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             5838..7487
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             7494..7511
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     misc_feature    complement(8426..8450)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10049..11194)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(11496..12287)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  12479..13246
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      complement(13947..14557)
                     /direction=LEFT
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
ORIGIN
        1 tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
     2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
     3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
     3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
     3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
     4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
     4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
     4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
     4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
     4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
     4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
     4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
     4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
     4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4921 caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc cccagattag
     4981 ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt acgcagcagg
     5041 tctcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc ttcccaagaa
     5101 ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat
     5161 atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc ttcacaaacc
     5221 aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat caaaggccat
     5281 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt
     5341 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca
     5401 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
     5461 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
     5521 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
     5581 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
     5641 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
     5701 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
     5761 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactctaga
     5821 ggatccccgg gggtaccatg gccgatgcta agaacattaa gaagggccct gctcccttct
     5881 accctctgga ggatggcacc gctggcgagc agctgcacaa ggccatgaag aggtatgccc
     5941 tggtgcctgg caccattgcc ttcaccgatg cccacattga ggtggacatc acctatgccg
     6001 agtacttcga gatgtctgtg cgcctggccg aggccatgaa gaggtacggc ctgaacacca
     6061 accaccgcat cgtggtgtgc tctgagaact ctctgcagtt cttcatgcca gtgctgggcg
     6121 ccctgttcat cggagtggcc gtggcccctg ctaacgacat ttacaacgag cgcgagctgc
     6181 tgaacagcat gggcatttct cagcctaccg tggtgttcgt gtctaagaag ggcctgcaga
     6241 agatcctgaa cgtgcagaag aagctgccta tcatccagaa gatcatcatc atggactcta
     6301 agaccgacta ccagggcttc cagagcatgt acacattcgt gacatctcat ctgcctcctg
     6361 gcttcaacga gtacgacttc gtgccagagt ctttcgacag ggacaaaacc attgccctga
     6421 tcatgaacag ctctgggtct accggcctgc ctaagggcgt ggccctgcct catcgcaccg
     6481 cctgtgtgcg cttctctcac gcccgcgacc ctattttcgg caaccagatc atccccgaca
     6541 ccgctattct gagcgtggtg ccattccacc acggcttcgg catgttcacc accctgggct
     6601 acctgatttg cggctttcgg gtggtgctga tgtaccgctt cgaggaggag ctgttcctgc
     6661 gcagcctgca agactacaaa attcagtctg ccctgctggt gccaaccctg ttcagcttct
     6721 tcgctaagag caccctgatc gacaagtacg acctgtctaa cctgcacgag attgcctctg
     6781 gcggcgcccc actgtctaag gaggtgggcg aagccgtggc caagcgcttt catctgccag
     6841 gcatccgcca gggctacggc ctgaccgaga caaccagcgc cattctgatt accccagagg
     6901 gcgacgacaa gcctggcgcc gtgggcaagg tggtgccatt cttcgaggcc aaggtggtgg
     6961 acctggacac cggcaagacc ctgggagtga accagcgcgg cgagctgtgt gtgcgcggcc
     7021 ctatgattat gtccggctac gtgaataacc ctgaggccac aaacgccctg atcgacaagg
     7081 acggctggct gcactctggc gacattgcct actgggacga ggacgagcac ttcttcatcg
     7141 tggaccgcct gaagtctctg atcaagtaca agggctacca ggtggcccca gccgagctgg
     7201 agtctatcct gctgcagcac cctaacattt tcgacgccgg agtggccggc ctgcccgacg
     7261 acgatgccgg cgagctgcct gccgccgtcg tcgtgctgga acacggcaag accatgaccg
     7321 agaaggagat cgtggactat gtggccagcc aggtgacaac cgccaagaag ctgcgcggcg
     7381 gagtggtgtt cgtggacgag gtgcccaagg gcctgaccgg caagctggac gcccgcaaga
     7441 tccgcgagat cctgatcaag gctaagaaag gcggcaagat cgccgtgctc gagcaccacc
     7501 accaccacca ctgagagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc
     7561 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac
     7621 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg
     7681 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac
     7741 taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt cactggccgt
     7801 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     7861 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     7921 acagttgcgc agcctgaatg gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     7981 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     8041 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
     8101 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     8161 gactcttgtt ccaaactgga acaacactca accctatctc gggctattct tttgatttat
     8221 aagggatttt gccgatttcg gaaccaccat caaacaggat tttcgcctgc tggggcaaac
     8281 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
     8341 gcccgtctca ctggtgaaaa gaaaaaccac cccagtacat taaaaacgtc cgcaatgtgt
     8401 tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc
     8461 aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag
     8521 tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga
     8581 tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag
     8641 ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcaaa tatcatctcc
     8701 ctcgcagaga tccgaattat cagccttctt attcatttct cgcttaaccg tgacaggctg
     8761 tcgatcttga gaactatgcc gacataatag gaaatcgctg gataaagccg ctgaggaagc
     8821 tgagtggcgc tatttcttta gaagtgaacg ttgacgatat caactcccct atccattgct
     8881 caccgaatgg tacaggtcgg ggacccgaag ttccgactgt cggcctgatg catccccggc
     8941 tgatcgaccc cagatctggg gctgagaaag cccagtaagg aaacaactgt aggttcgagt
     9001 cgcgagatcc cccggaacca aaggaagtag gttaaacccg ctccgatcag gccgagccac
     9061 gccaggccga gaacattggt tcctgtaggc atcgggattg gcggatcaaa cactaaagct
     9121 actggaacga gcagaagtcc tccggccgcc agttgccagg cggtaaaggt gagcagaggc
     9181 acgggaggtt gccacttgcg ggtcagcacg gttccgaacg ccatggaaac cgcccccgcc
     9241 aggcccgctg cgacgccgac aggatctagc gctgcgtttg gtgtcaacac caacagcgcc
     9301 acgcccgcag ttccgcaaat agcccccagg accgccatca atcgtatcgg gctacctagc
     9361 agagcggcag agatgaacac gaccatcagc ggctgcacag cgcctaccgt cgccgcgacc
     9421 ccgcccggca ggcggtagac cgaaataaac aacaagctcc agaatagcga aatattaagt
     9481 gcgccgagga tgaagatgcg catccaccag attcccgttg gaatctgtcg gacgatcatc
     9541 acgagcaata aacccgccgg caacgcccgc agcagcatac cggcgacccc tcggcctcgc
     9601 tgttcgggct ccacgaaaac gccggacaga tgcgccttgt gagcgtcctt ggggccgtcc
     9661 tcctgtttga agaccgacag cccaatgatc tcgccgtcga tgtaggcgcc gaatgccacg
     9721 gcatctcgca accgttcagc gaacgcctcc atgggctttt tctcctcgtg ctcgtaaacg
     9781 gacccgaaca tctctggagc tttcttcagg gccgacaatc ggatctcgcg gaaatcctgc
     9841 acgtcggccg ctccaagccg tcgaatctga gccttaatca caattgtcaa ttttaatcct
     9901 ctgtttatcg gcagttcgta gagcgcgccg tgcgtcccga gcgatactga gcgaagcaag
     9961 tgcgtcgagc agtgcccgct tgttcctgaa atgccagtaa agcgctggct gctgaacccc
    10021 cagccggaac tgaccccaca aggccctagc gtttgcaatg caccaggtca tcattgaccc
    10081 aggcgtgttc caccaggccg ctgcctcgca actcttcgca ggcttcgccg acctgctcgc
    10141 gccacttctt cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt tccagcttga
    10201 gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc gtcggcgaca
    10261 gcttgcggta cttctcccat atgaatttcg tgtagtggtc gccagcaaac agcacgacga
    10321 tttcctcgtc gatcaggacc tggcaacggg acgttttctt gccacggtcc aggacgcgga
    10381 agcggtgcag cagcgacacc gattccaggt gcccaacgcg gtcggacgtg aagcccatcg
    10441 ccgtcgcctg taggcgcgac aggcattcct cggccttcgt gtaataccgg ccattgatcg
    10501 accagcccag gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg ccgatagggg
    10561 tgcgcttcgc gtactccaac acctgctgcc acaccagttc gtcatcgtcg gcccgcagct
    10621 cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc ttgttttgca
    10681 gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg gccgtgtcgt
    10741 ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa agctgcattt
    10801 ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg ctgacctgtt
    10861 ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt catagttcct cgcgtgtcga
    10921 tggtcatcga cttcgccaaa cctgccgcct cctgttcgag acgacgcgaa cgctccacgg
    10981 cggccgatgg cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc tcgatcttgg
    11041 ccgtagcttg ctggaccatc gagccgacgg actggaaggt ttcgcggggc gcacgcatga
    11101 cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg atcagttctt
    11161 gcctgtatgc cttccggtca aacgtccgat tcattcaccc tccttgcggg attgccccga
    11221 ctcacgccgg ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc ttggtgtcca
    11281 gataatccac cttatcggca atgaagtcgg tcccgtagac cgtctggccg tccttctcgt
    11341 acttggtatt ccgaatcttg ccctgcacga ataccagcga ccccttgccc aaatacttgc
    11401 cgtgggcctc ggcctgagag ccaaaacact tgatgcggaa gaagtcggtg cgctcctgct
    11461 tgtcgccggc atcgttgcgc cacatctagg tactaaaaca attcatccag taaaatataa
    11521 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac
    11581 tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg
    11641 tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag
    11701 atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc
    11761 tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg
    11821 caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct
    11881 aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc
    11941 gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg
    12001 acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg
    12061 acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca
    12121 tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct
    12181 cccaccagct tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat
    12241 ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt
    12301 cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca
    12361 attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt
    12421 tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaacca caattatggg
    12481 tgatgctgcc aacttactga tttagtgtat gatggtgttt ttgaggtgct ccagtggctt
    12541 ctgtgtctat cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa
    12601 gcaccgccgg acatcagcgc tatctctgct ctcactgccg taaaacatgg caactgcagt
    12661 tcacttacac cgcttctcaa cccggtacgc accagaaaat cattgatatg gccatgaatg
    12721 gcgttggatg ccgggcaaca gcccgcatta tgggcgttgg cctcaacacg attttacgtc
    12781 acttaaaaaa ctcaggccgc agtcggtaac ctcgcgcata cagccgggca gtgacgtcat
    12841 cgtctgcgcg gaaatggacg aacagtgggg ctatgtcggg gctaaatcgc gccagcgctg
    12901 gctgttttac gcgtatgaca gtctccggaa gacggttgtt gcgcacgtat tcggtgaacg
    12961 cactatggcg acgctggggc gtcttatgag cctgctgtca ccctttgacg tggtgatatg
    13021 gatgacggat ggctggccgc tgtatgaatc ccgcctgaag ggaaagctgc acgtaatcag
    13081 caagcgatat acgcagcgaa ttgagcggca taacctgaat ctgaggcagc acctggcacg
    13141 gctgggacgg aagtcgctgt cgttctcaaa atcggtggag ctgcatgaca aagtcatcgg
    13201 gcattatctg aacataaaac actatcaata agttggagtc attacccaat tatgatagaa
    13261 tttacaagct ataaggttat tgtcctgggt ttcaagcatt agtccatgca agtttttatg
    13321 ctttgcccat tctatagata tattgataag cgcgctgcct atgccttgcc ccctgaaatc
    13381 cttacatacg gcgatatctt ctatataaaa gatatattat cttatcagta ttgtcaatat
    13441 attcaaggca atctgcctcc tcatcctctt catcctcttc gtcttggtag ctttttaaat
    13501 atggcgcttc atagagtaat tctgtaaagg tccaattctc gttttcatac ctcggtataa
    13561 tcttacctat cacctcaaat ggttcgctgg gtttatcgca cccccgaaca cgagcacggc
    13621 acccgcgacc actatgccaa gaatgcccaa ggtaaaaatt gccggccccg ccatgaagtc
    13681 cgtgaatgcc ccgacggccg aagtgaaggg caggccgcca cccaggccgc cgccctcact
    13741 gcccggcacc tggtcgctga atgtcgatgc cagcacctgc ggcacgtcaa tgcttccggg
    13801 cgtcgcgctc gggctgatcg cccatcccgt tactgccccg atcccggcaa tggcaaggac
    13861 tgccagcgct gccatttttg gggtgaggcc gttcgcggcc gaggggcgca gcccctgggg
    13921 ggatgggagg cccgcgttag cgggccggga gggttcgaga agggggggca ccccccttcg
    13981 gcgtgcgcgg tcacgcgcac agggcgcagc cctggttaaa aacaaggttt ataaatattg
    14041 gtttaaaagc aggttaaaag acaggttagc ggtggccgaa aaacgggcgg aaacccttgc
    14101 aaatgctgga ttttctgcct gtggacagcc cctcaaatgt caataggtgc gcccctcatc
    14161 tgtcagcact ctgcccctca agtgtcaagg atcgcgcccc tcatctgtca gtagtcgcgc
    14221 ccctcaagtg tcaataccgc agggcactta tccccaggct tgtccacatc atctgtggga
    14281 aactcgcgta aaatcaggcg ttttcgccga tttgcgaggc tggccagctc cacgtcgccg
    14341 gccgaaatcg agcctgcccc tcatctgtca acgccgcgcc gggtgagtcg gcccctcaag
    14401 tgtcaacgtc cgcccctcat ctgtcagtga gggccaagtt ttccgcgagg tatccacaac
    14461 gccggcggcc gcggtgtctc gcacacggct tcgacggcgt ttctggcgcg tttgcagggc
    14521 catagacggc cgccagccca gcggcgaggg caaccagccc gg
//
