LOCUS       62056_3925       13829 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13829)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..13829
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(148..516)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(549..658)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(1342..1989)
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    complement(2454..2478)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2634..2817
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     rep_origin      complement(3206..3830)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     terminator      4022..4274
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4823..4844
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4859..4889
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        5461..5806
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             6035..6751
                     /codon_start=1
                     /label=CFP
                     /note="cyan variant of GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             6761..6778
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     misc_feature    complement(7693..7717)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(9316..10461)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(10763..11554)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  11746..12513
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      complement(13214..13824)
                     /direction=LEFT
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
ORIGIN
        1 tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
     2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
     3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
     3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
     3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
     4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
     4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
     4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
     4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
     4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
     4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
     4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
     4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
     4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4921 caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc cccagattag
     4981 ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt acgcagcagg
     5041 tctcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc ttcccaagaa
     5101 ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat
     5161 atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc ttcacaaacc
     5221 aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat caaaggccat
     5281 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt
     5341 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca
     5401 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
     5461 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
     5521 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
     5581 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
     5641 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
     5701 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
     5761 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactctaga
     5821 atgggtagca agatgttgtt tagtttgaca agtcctcgac ttttctccgc cgtttctcgc
     5881 aaaccttcct cttctttctc tccttctcct ccgtcgccgt cttcgaggac tcaatggact
     5941 cagctcagcc ctggaaaatc gatttctttg agaagaagag tcttcttgtt gcctgctaaa
     6001 gccacaacag agcaatcagt agggatccgg taccatggtg agcaagggcg aggagctgtt
     6061 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaggttcag
     6121 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     6181 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctggggcgt
     6241 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     6301 gcccgaaggc tacgtccagg agcgtaccat cttcttcaag gacgacggca actacaagac
     6361 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     6421 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acatcagcca
     6481 caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggcccact tcaagatccg
     6541 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     6601 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     6661 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     6721 gatcactctc ggcatggacg agctgtacaa gcaactcgag caccaccacc accaccactg
     6781 agagctcgaa tttccccgat cgttcaaaca tttggcaata aagtttctta agattgaatc
     6841 ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa
     6901 taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc
     6961 aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat
     7021 cgcgcgcggt gtcatctatg ttactagatc gggaattcac tggccgtcgt tttacaacgt
     7081 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     7141 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     7201 ctgaatggcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     7261 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     7321 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata
     7381 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     7441 aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag ggattttgcc
     7501 gatttcggaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc
     7561 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg
     7621 gtgaaaagaa aaaccacccc agtacattaa aaacgtccgc aatgtgttat taagttgtct
     7681 aagcgtcaat ttgtttacac cacaatatat cctgccacca gccagccaac agctccccga
     7741 ccggcagctc ggcacaaaat caccactcga tacaggcagc ccatcagtcc gggacggcgt
     7801 cagcgggaga gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc tattcggaag
     7861 aacggcaact aagctgccgg gtttgaaaca cggatgatct cgcggagggt agcatgttga
     7921 ttgtaacgat gacagagcgt tgctgcctgt gatcaaatat catctccctc gcagagatcc
     7981 gaattatcag ccttcttatt catttctcgc ttaaccgtga caggctgtcg atcttgagaa
     8041 ctatgccgac ataataggaa atcgctggat aaagccgctg aggaagctga gtggcgctat
     8101 ttctttagaa gtgaacgttg acgatatcaa ctcccctatc cattgctcac cgaatggtac
     8161 aggtcgggga cccgaagttc cgactgtcgg cctgatgcat ccccggctga tcgaccccag
     8221 atctggggct gagaaagccc agtaaggaaa caactgtagg ttcgagtcgc gagatccccc
     8281 ggaaccaaag gaagtaggtt aaacccgctc cgatcaggcc gagccacgcc aggccgagaa
     8341 cattggttcc tgtaggcatc gggattggcg gatcaaacac taaagctact ggaacgagca
     8401 gaagtcctcc ggccgccagt tgccaggcgg taaaggtgag cagaggcacg ggaggttgcc
     8461 acttgcgggt cagcacggtt ccgaacgcca tggaaaccgc ccccgccagg cccgctgcga
     8521 cgccgacagg atctagcgct gcgtttggtg tcaacaccaa cagcgccacg cccgcagttc
     8581 cgcaaatagc ccccaggacc gccatcaatc gtatcgggct acctagcaga gcggcagaga
     8641 tgaacacgac catcagcggc tgcacagcgc ctaccgtcgc cgcgaccccg cccggcaggc
     8701 ggtagaccga aataaacaac aagctccaga atagcgaaat attaagtgcg ccgaggatga
     8761 agatgcgcat ccaccagatt cccgttggaa tctgtcggac gatcatcacg agcaataaac
     8821 ccgccggcaa cgcccgcagc agcataccgg cgacccctcg gcctcgctgt tcgggctcca
     8881 cgaaaacgcc ggacagatgc gccttgtgag cgtccttggg gccgtcctcc tgtttgaaga
     8941 ccgacagccc aatgatctcg ccgtcgatgt aggcgccgaa tgccacggca tctcgcaacc
     9001 gttcagcgaa cgcctccatg ggctttttct cctcgtgctc gtaaacggac ccgaacatct
     9061 ctggagcttt cttcagggcc gacaatcgga tctcgcggaa atcctgcacg tcggccgctc
     9121 caagccgtcg aatctgagcc ttaatcacaa ttgtcaattt taatcctctg tttatcggca
     9181 gttcgtagag cgcgccgtgc gtcccgagcg atactgagcg aagcaagtgc gtcgagcagt
     9241 gcccgcttgt tcctgaaatg ccagtaaagc gctggctgct gaacccccag ccggaactga
     9301 ccccacaagg ccctagcgtt tgcaatgcac caggtcatca ttgacccagg cgtgttccac
     9361 caggccgctg cctcgcaact cttcgcaggc ttcgccgacc tgctcgcgcc acttcttcac
     9421 gcgggtggaa tccgatccgc acatgaggcg gaaggtttcc agcttgagcg ggtacggctc
     9481 ccggtgcgag ctgaaatagt cgaacatccg tcgggccgtc ggcgacagct tgcggtactt
     9541 ctcccatatg aatttcgtgt agtggtcgcc agcaaacagc acgacgattt cctcgtcgat
     9601 caggacctgg caacgggacg ttttcttgcc acggtccagg acgcggaagc ggtgcagcag
     9661 cgacaccgat tccaggtgcc caacgcggtc ggacgtgaag cccatcgccg tcgcctgtag
     9721 gcgcgacagg cattcctcgg ccttcgtgta ataccggcca ttgatcgacc agcccaggtc
     9781 ctggcaaagc tcgtagaacg tgaaggtgat cggctcgccg ataggggtgc gcttcgcgta
     9841 ctccaacacc tgctgccaca ccagttcgtc atcgtcggcc cgcagctcga cgccggtgta
     9901 ggtgatcttc acgtccttgt tgacgtggaa aatgaccttg ttttgcagcg cctcgcgcgg
     9961 gattttcttg ttgcgcgtgg tgaacagggc agagcgggcc gtgtcgtttg gcatcgctcg
    10021 catcgtgtcc ggccacggcg caatatcgaa caaggaaagc tgcatttcct tgatctgctg
    10081 cttcgtgtgt ttcagcaacg cggcctgctt ggcctcgctg acctgttttg ccaggtcctc
    10141 gccggcggtt tttcgcttct tggtcgtcat agttcctcgc gtgtcgatgg tcatcgactt
    10201 cgccaaacct gccgcctcct gttcgagacg acgcgaacgc tccacggcgg ccgatggcgc
    10261 gggcagggca gggggagcca gttgcacgct gtcgcgctcg atcttggccg tagcttgctg
    10321 gaccatcgag ccgacggact ggaaggtttc gcggggcgca cgcatgacgg tgcggcttgc
    10381 gatggtttcg gcatcctcgg cggaaaaccc cgcgtcgatc agttcttgcc tgtatgcctt
    10441 ccggtcaaac gtccgattca ttcaccctcc ttgcgggatt gccccgactc acgccggggc
    10501 aatgtgccct tattcctgat ttgacccgcc tggtgccttg gtgtccagat aatccacctt
    10561 atcggcaatg aagtcggtcc cgtagaccgt ctggccgtcc ttctcgtact tggtattccg
    10621 aatcttgccc tgcacgaata ccagcgaccc cttgcccaaa tacttgccgt gggcctcggc
    10681 ctgagagcca aaacacttga tgcggaagaa gtcggtgcgc tcctgcttgt cgccggcatc
    10741 gttgcgccac atctaggtac taaaacaatt catccagtaa aatataatat tttattttct
    10801 cccaatcagg cttgatcccc agtaagtcaa aaaatagctc gacatactgt tcttccccga
    10861 tatcctccct gatcgaccgg acgcagaagg caatgtcata ccacttgtcc gccctgccgc
    10921 ttctcccaag atcaataaag ccacttactt tgccatcttt cacaaagatg ttgctgtctc
    10981 ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt ttccgtcttt aaaaaatcat
    11041 acagctcgcg cggatcttta aatggagtgt cttcttccca gttttcgcaa tccacatcgg
    11101 ccagatcgtt attcagtaag taatccaatt cggctaagcg gctgtctaag ctattcgtat
    11161 agggacaatc cgatatgtcg atggagtgaa agagcctgat gcactccgca tacagctcga
    11221 taatcttttc agggctttgt tcatcttcat actcttccga gcaaaggacg ccatcggcct
    11281 cactcatgag cagattgctc cagccatcat gccgttcaaa gtgcaggacc tttggaacag
    11341 gcagctttcc ttccagccat agcatcatgt ccttttcccg ttccacatca taggtggtcc
    11401 ctttataccg gctgtccgtc atttttaaat ataggttttc attttctccc accagcttat
    11461 ataccttagc aggagacatt ccttccgtat cttttacgca gcggtatttt tcgatcagtt
    11521 ttttcaattc cggtgatatt ctcattttag ccatttatta tttccttcct cttttctaca
    11581 gtatttaaag ataccccaag aagctaatta taacaagacg aactccaatt cactgttcct
    11641 tgcattctaa aaccttaaat accagaaaac agctttttca aagttgtttt caaagttggc
    11701 gtataacata gtatcgacgg agccgatttt gaaaccacaa ttatgggtga tgctgccaac
    11761 ttactgattt agtgtatgat ggtgtttttg aggtgctcca gtggcttctg tgtctatcag
    11821 ctgtccctcc tgttcagcta ctgacggggt ggtgcgtaac ggcaaaagca ccgccggaca
    11881 tcagcgctat ctctgctctc actgccgtaa aacatggcaa ctgcagttca cttacaccgc
    11941 ttctcaaccc ggtacgcacc agaaaatcat tgatatggcc atgaatggcg ttggatgccg
    12001 ggcaacagcc cgcattatgg gcgttggcct caacacgatt ttacgtcact taaaaaactc
    12061 aggccgcagt cggtaacctc gcgcatacag ccgggcagtg acgtcatcgt ctgcgcggaa
    12121 atggacgaac agtggggcta tgtcggggct aaatcgcgcc agcgctggct gttttacgcg
    12181 tatgacagtc tccggaagac ggttgttgcg cacgtattcg gtgaacgcac tatggcgacg
    12241 ctggggcgtc ttatgagcct gctgtcaccc tttgacgtgg tgatatggat gacggatggc
    12301 tggccgctgt atgaatcccg cctgaaggga aagctgcacg taatcagcaa gcgatatacg
    12361 cagcgaattg agcggcataa cctgaatctg aggcagcacc tggcacggct gggacggaag
    12421 tcgctgtcgt tctcaaaatc ggtggagctg catgacaaag tcatcgggca ttatctgaac
    12481 ataaaacact atcaataagt tggagtcatt acccaattat gatagaattt acaagctata
    12541 aggttattgt cctgggtttc aagcattagt ccatgcaagt ttttatgctt tgcccattct
    12601 atagatatat tgataagcgc gctgcctatg ccttgccccc tgaaatcctt acatacggcg
    12661 atatcttcta tataaaagat atattatctt atcagtattg tcaatatatt caaggcaatc
    12721 tgcctcctca tcctcttcat cctcttcgtc ttggtagctt tttaaatatg gcgcttcata
    12781 gagtaattct gtaaaggtcc aattctcgtt ttcatacctc ggtataatct tacctatcac
    12841 ctcaaatggt tcgctgggtt tatcgcaccc ccgaacacga gcacggcacc cgcgaccact
    12901 atgccaagaa tgcccaaggt aaaaattgcc ggccccgcca tgaagtccgt gaatgccccg
    12961 acggccgaag tgaagggcag gccgccaccc aggccgccgc cctcactgcc cggcacctgg
    13021 tcgctgaatg tcgatgccag cacctgcggc acgtcaatgc ttccgggcgt cgcgctcggg
    13081 ctgatcgccc atcccgttac tgccccgatc ccggcaatgg caaggactgc cagcgctgcc
    13141 atttttgggg tgaggccgtt cgcggccgag gggcgcagcc cctgggggga tgggaggccc
    13201 gcgttagcgg gccgggaggg ttcgagaagg gggggcaccc cccttcggcg tgcgcggtca
    13261 cgcgcacagg gcgcagccct ggttaaaaac aaggtttata aatattggtt taaaagcagg
    13321 ttaaaagaca ggttagcggt ggccgaaaaa cgggcggaaa cccttgcaaa tgctggattt
    13381 tctgcctgtg gacagcccct caaatgtcaa taggtgcgcc cctcatctgt cagcactctg
    13441 cccctcaagt gtcaaggatc gcgcccctca tctgtcagta gtcgcgcccc tcaagtgtca
    13501 ataccgcagg gcacttatcc ccaggcttgt ccacatcatc tgtgggaaac tcgcgtaaaa
    13561 tcaggcgttt tcgccgattt gcgaggctgg ccagctccac gtcgccggcc gaaatcgagc
    13621 ctgcccctca tctgtcaacg ccgcgccggg tgagtcggcc cctcaagtgt caacgtccgc
    13681 ccctcatctg tcagtgaggg ccaagttttc cgcgaggtat ccacaacgcc ggcggccgcg
    13741 gtgtctcgca cacggcttcg acggcgtttc tggcgcgttt gcagggccat agacggccgc
    13801 cagcccagcg gcgagggcaa ccagcccgg
//
