LOCUS       62056_3920       13632 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13632)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..13632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(148..516)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(549..658)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(1342..1989)
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    complement(2454..2478)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2634..2817
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     rep_origin      complement(3009..3633)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     terminator      4022..4274
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4823..4844
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4859..4889
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        5461..5806
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             5838..6554
                     /codon_start=1
                     /label=CFP
                     /note="cyan variant of GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             6564..6581
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     misc_feature    complement(7496..7520)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(9119..10264)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(10566..11357)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  11549..12316
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      complement(13017..13627)
                     /direction=LEFT
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
ORIGIN
        1 tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
     2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
     3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
     3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
     3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
     4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
     4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
     4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
     4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
     4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
     4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
     4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
     4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
     4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4921 caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc cccagattag
     4981 ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt acgcagcagg
     5041 tctcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc ttcccaagaa
     5101 ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat
     5161 atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc ttcacaaacc
     5221 aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat caaaggccat
     5281 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt
     5341 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca
     5401 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
     5461 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
     5521 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
     5581 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
     5641 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
     5701 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
     5761 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactctaga
     5821 ggatccccgg gggtaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     5881 tcctggtcga gctggacggc gacgtaaacg gccacaggtt cagcgtgtcc ggcgagggcg
     5941 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     6001 ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc ttcagccgct
     6061 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     6121 aggagcgtac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     6181 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     6241 gcaacatcct ggggcacaag ctggagtaca actacatcag ccacaacgtc tatatcaccg
     6301 ccgacaagca gaagaacggc atcaaggccc acttcaagat ccgccacaac atcgaggacg
     6361 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     6421 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     6481 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     6541 acgagctgta caagcaactc gagcaccacc accaccacca ctgagagctc gaatttcccc
     6601 gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg
     6661 atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc
     6721 atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac
     6781 gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct
     6841 atgttactag atcgggaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     6901 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     6961 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgcccgctc
     7021 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     7081 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     7141 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     7201 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     7261 accctatctc gggctattct tttgatttat aagggatttt gccgatttcg gaaccaccat
     7321 caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca
     7381 gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac
     7441 cccagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttgttta
     7501 caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag ctcggcacaa
     7561 aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg agagccgttg
     7621 taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc
     7681 cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag
     7741 cgttgctgcc tgtgatcaaa tatcatctcc ctcgcagaga tccgaattat cagccttctt
     7801 attcatttct cgcttaaccg tgacaggctg tcgatcttga gaactatgcc gacataatag
     7861 gaaatcgctg gataaagccg ctgaggaagc tgagtggcgc tatttcttta gaagtgaacg
     7921 ttgacgatat caactcccct atccattgct caccgaatgg tacaggtcgg ggacccgaag
     7981 ttccgactgt cggcctgatg catccccggc tgatcgaccc cagatctggg gctgagaaag
     8041 cccagtaagg aaacaactgt aggttcgagt cgcgagatcc cccggaacca aaggaagtag
     8101 gttaaacccg ctccgatcag gccgagccac gccaggccga gaacattggt tcctgtaggc
     8161 atcgggattg gcggatcaaa cactaaagct actggaacga gcagaagtcc tccggccgcc
     8221 agttgccagg cggtaaaggt gagcagaggc acgggaggtt gccacttgcg ggtcagcacg
     8281 gttccgaacg ccatggaaac cgcccccgcc aggcccgctg cgacgccgac aggatctagc
     8341 gctgcgtttg gtgtcaacac caacagcgcc acgcccgcag ttccgcaaat agcccccagg
     8401 accgccatca atcgtatcgg gctacctagc agagcggcag agatgaacac gaccatcagc
     8461 ggctgcacag cgcctaccgt cgccgcgacc ccgcccggca ggcggtagac cgaaataaac
     8521 aacaagctcc agaatagcga aatattaagt gcgccgagga tgaagatgcg catccaccag
     8581 attcccgttg gaatctgtcg gacgatcatc acgagcaata aacccgccgg caacgcccgc
     8641 agcagcatac cggcgacccc tcggcctcgc tgttcgggct ccacgaaaac gccggacaga
     8701 tgcgccttgt gagcgtcctt ggggccgtcc tcctgtttga agaccgacag cccaatgatc
     8761 tcgccgtcga tgtaggcgcc gaatgccacg gcatctcgca accgttcagc gaacgcctcc
     8821 atgggctttt tctcctcgtg ctcgtaaacg gacccgaaca tctctggagc tttcttcagg
     8881 gccgacaatc ggatctcgcg gaaatcctgc acgtcggccg ctccaagccg tcgaatctga
     8941 gccttaatca caattgtcaa ttttaatcct ctgtttatcg gcagttcgta gagcgcgccg
     9001 tgcgtcccga gcgatactga gcgaagcaag tgcgtcgagc agtgcccgct tgttcctgaa
     9061 atgccagtaa agcgctggct gctgaacccc cagccggaac tgaccccaca aggccctagc
     9121 gtttgcaatg caccaggtca tcattgaccc aggcgtgttc caccaggccg ctgcctcgca
     9181 actcttcgca ggcttcgccg acctgctcgc gccacttctt cacgcgggtg gaatccgatc
     9241 cgcacatgag gcggaaggtt tccagcttga gcgggtacgg ctcccggtgc gagctgaaat
     9301 agtcgaacat ccgtcgggcc gtcggcgaca gcttgcggta cttctcccat atgaatttcg
     9361 tgtagtggtc gccagcaaac agcacgacga tttcctcgtc gatcaggacc tggcaacggg
     9421 acgttttctt gccacggtcc aggacgcgga agcggtgcag cagcgacacc gattccaggt
     9481 gcccaacgcg gtcggacgtg aagcccatcg ccgtcgcctg taggcgcgac aggcattcct
     9541 cggccttcgt gtaataccgg ccattgatcg accagcccag gtcctggcaa agctcgtaga
     9601 acgtgaaggt gatcggctcg ccgatagggg tgcgcttcgc gtactccaac acctgctgcc
     9661 acaccagttc gtcatcgtcg gcccgcagct cgacgccggt gtaggtgatc ttcacgtcct
     9721 tgttgacgtg gaaaatgacc ttgttttgca gcgcctcgcg cgggattttc ttgttgcgcg
     9781 tggtgaacag ggcagagcgg gccgtgtcgt ttggcatcgc tcgcatcgtg tccggccacg
     9841 gcgcaatatc gaacaaggaa agctgcattt ccttgatctg ctgcttcgtg tgtttcagca
     9901 acgcggcctg cttggcctcg ctgacctgtt ttgccaggtc ctcgccggcg gtttttcgct
     9961 tcttggtcgt catagttcct cgcgtgtcga tggtcatcga cttcgccaaa cctgccgcct
    10021 cctgttcgag acgacgcgaa cgctccacgg cggccgatgg cgcgggcagg gcagggggag
    10081 ccagttgcac gctgtcgcgc tcgatcttgg ccgtagcttg ctggaccatc gagccgacgg
    10141 actggaaggt ttcgcggggc gcacgcatga cggtgcggct tgcgatggtt tcggcatcct
    10201 cggcggaaaa ccccgcgtcg atcagttctt gcctgtatgc cttccggtca aacgtccgat
    10261 tcattcaccc tccttgcggg attgccccga ctcacgccgg ggcaatgtgc ccttattcct
    10321 gatttgaccc gcctggtgcc ttggtgtcca gataatccac cttatcggca atgaagtcgg
    10381 tcccgtagac cgtctggccg tccttctcgt acttggtatt ccgaatcttg ccctgcacga
    10441 ataccagcga ccccttgccc aaatacttgc cgtgggcctc ggcctgagag ccaaaacact
    10501 tgatgcggaa gaagtcggtg cgctcctgct tgtcgccggc atcgttgcgc cacatctagg
    10561 tactaaaaca attcatccag taaaatataa tattttattt tctcccaatc aggcttgatc
    10621 cccagtaagt caaaaaatag ctcgacatac tgttcttccc cgatatcctc cctgatcgac
    10681 cggacgcaga aggcaatgtc ataccacttg tccgccctgc cgcttctccc aagatcaata
    10741 aagccactta ctttgccatc tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa
    10801 aagacaagtt cctcttcggg cttttccgtc tttaaaaaat catacagctc gcgcggatct
    10861 ttaaatggag tgtcttcttc ccagttttcg caatccacat cggccagatc gttattcagt
    10921 aagtaatcca attcggctaa gcggctgtct aagctattcg tatagggaca atccgatatg
    10981 tcgatggagt gaaagagcct gatgcactcc gcatacagct cgataatctt ttcagggctt
    11041 tgttcatctt catactcttc cgagcaaagg acgccatcgg cctcactcat gagcagattg
    11101 ctccagccat catgccgttc aaagtgcagg acctttggaa caggcagctt tccttccagc
    11161 catagcatca tgtccttttc ccgttccaca tcataggtgg tccctttata ccggctgtcc
    11221 gtcattttta aatataggtt ttcattttct cccaccagct tatatacctt agcaggagac
    11281 attccttccg tatcttttac gcagcggtat ttttcgatca gttttttcaa ttccggtgat
    11341 attctcattt tagccattta ttatttcctt cctcttttct acagtattta aagatacccc
    11401 aagaagctaa ttataacaag acgaactcca attcactgtt ccttgcattc taaaacctta
    11461 aataccagaa aacagctttt tcaaagttgt tttcaaagtt ggcgtataac atagtatcga
    11521 cggagccgat tttgaaacca caattatggg tgatgctgcc aacttactga tttagtgtat
    11581 gatggtgttt ttgaggtgct ccagtggctt ctgtgtctat cagctgtccc tcctgttcag
    11641 ctactgacgg ggtggtgcgt aacggcaaaa gcaccgccgg acatcagcgc tatctctgct
    11701 ctcactgccg taaaacatgg caactgcagt tcacttacac cgcttctcaa cccggtacgc
    11761 accagaaaat cattgatatg gccatgaatg gcgttggatg ccgggcaaca gcccgcatta
    11821 tgggcgttgg cctcaacacg attttacgtc acttaaaaaa ctcaggccgc agtcggtaac
    11881 ctcgcgcata cagccgggca gtgacgtcat cgtctgcgcg gaaatggacg aacagtgggg
    11941 ctatgtcggg gctaaatcgc gccagcgctg gctgttttac gcgtatgaca gtctccggaa
    12001 gacggttgtt gcgcacgtat tcggtgaacg cactatggcg acgctggggc gtcttatgag
    12061 cctgctgtca ccctttgacg tggtgatatg gatgacggat ggctggccgc tgtatgaatc
    12121 ccgcctgaag ggaaagctgc acgtaatcag caagcgatat acgcagcgaa ttgagcggca
    12181 taacctgaat ctgaggcagc acctggcacg gctgggacgg aagtcgctgt cgttctcaaa
    12241 atcggtggag ctgcatgaca aagtcatcgg gcattatctg aacataaaac actatcaata
    12301 agttggagtc attacccaat tatgatagaa tttacaagct ataaggttat tgtcctgggt
    12361 ttcaagcatt agtccatgca agtttttatg ctttgcccat tctatagata tattgataag
    12421 cgcgctgcct atgccttgcc ccctgaaatc cttacatacg gcgatatctt ctatataaaa
    12481 gatatattat cttatcagta ttgtcaatat attcaaggca atctgcctcc tcatcctctt
    12541 catcctcttc gtcttggtag ctttttaaat atggcgcttc atagagtaat tctgtaaagg
    12601 tccaattctc gttttcatac ctcggtataa tcttacctat cacctcaaat ggttcgctgg
    12661 gtttatcgca cccccgaaca cgagcacggc acccgcgacc actatgccaa gaatgcccaa
    12721 ggtaaaaatt gccggccccg ccatgaagtc cgtgaatgcc ccgacggccg aagtgaaggg
    12781 caggccgcca cccaggccgc cgccctcact gcccggcacc tggtcgctga atgtcgatgc
    12841 cagcacctgc ggcacgtcaa tgcttccggg cgtcgcgctc gggctgatcg cccatcccgt
    12901 tactgccccg atcccggcaa tggcaaggac tgccagcgct gccatttttg gggtgaggcc
    12961 gttcgcggcc gaggggcgca gcccctgggg ggatgggagg cccgcgttag cgggccggga
    13021 gggttcgaga agggggggca ccccccttcg gcgtgcgcgg tcacgcgcac agggcgcagc
    13081 cctggttaaa aacaaggttt ataaatattg gtttaaaagc aggttaaaag acaggttagc
    13141 ggtggccgaa aaacgggcgg aaacccttgc aaatgctgga ttttctgcct gtggacagcc
    13201 cctcaaatgt caataggtgc gcccctcatc tgtcagcact ctgcccctca agtgtcaagg
    13261 atcgcgcccc tcatctgtca gtagtcgcgc ccctcaagtg tcaataccgc agggcactta
    13321 tccccaggct tgtccacatc atctgtggga aactcgcgta aaatcaggcg ttttcgccga
    13381 tttgcgaggc tggccagctc cacgtcgccg gccgaaatcg agcctgcccc tcatctgtca
    13441 acgccgcgcc gggtgagtcg gcccctcaag tgtcaacgtc cgcccctcat ctgtcagtga
    13501 gggccaagtt ttccgcgagg tatccacaac gccggcggcc gcggtgtctc gcacacggct
    13561 tcgacggcgt ttctggcgcg tttgcagggc catagacggc cgccagccca gcggcgaggg
    13621 caaccagccc gg
//
