LOCUS       62056_3910       14214 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14214)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..14214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(148..516)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(549..658)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(1342..1989)
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    complement(2454..2478)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2634..2817
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2841..3629
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     rep_origin      complement(3591..4215)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     protein_bind    4823..4844
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4859..4889
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        5461..5806
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             5838..6554
                     /codon_start=1
                     /label=CFP
                     /note="cyan variant of GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    complement(8078..8102)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(9701..10846)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(11148..11939)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  12131..12898
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      complement(13599..14209)
                     /direction=LEFT
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
ORIGIN
        1 tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
     2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
     3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
     3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
     3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
     4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
     4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
     4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
     4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
     4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
     4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
     4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
     4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
     4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4921 caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc cccagattag
     4981 ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt acgcagcagg
     5041 tctcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc ttcccaagaa
     5101 ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat
     5161 atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc ttcacaaacc
     5221 aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat caaaggccat
     5281 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt
     5341 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca
     5401 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
     5461 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
     5521 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
     5581 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
     5641 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
     5701 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
     5761 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactctaga
     5821 ggatccccgg gggtaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     5881 tcctggtcga gctggacggc gacgtaaacg gccacaggtt cagcgtgtcc ggcgagggcg
     5941 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     6001 ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc ttcagccgct
     6061 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     6121 aggagcgtac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     6181 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     6241 gcaacatcct ggggcacaag ctggagtaca actacatcag ccacaacgtc tatatcaccg
     6301 ccgacaagca gaagaacggc atcaaggccc acttcaagat ccgccacaac atcgaggacg
     6361 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     6421 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     6481 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     6541 acgagctgta caagcaactc gagatggcta gctctggaaa caagaacatc aacgccaaat
     6601 tggtattact aggagatgtt ggagctggaa aatcaagtct tgtgctacgg tttgtgaaag
     6661 atcagtttgt tgaatttcag gaatcaacca ttggtgcagc ttttttctct caaacattgg
     6721 ctgtgaatga tgcgactgtg aagtttgaga tatgggatac agctggtcag gaacgatacc
     6781 acagtttggc tccaatgtac tacaggggtg cagctgctgc tattattgtc tttgacatta
     6841 ctaatcaagc ctcatttgag agggcgaaga aatgggttca ggaactgcag gcacaaggta
     6901 accctaatat ggtgatggct cttgctggaa acaaagctga tttattagac gcaaggaagg
     6961 tgtctgcaga ggaggcagag atatatgctc aagagaacag ccttttcttt atggaaacct
     7021 cagcgaagac cgcaacaaat gtcaaagaca tattctacga aatagcgaaa aggctaccac
     7081 gtgtacagcc agctgaaaac ccgacaggaa tggttctccc aaacgggcca ggggctacgg
     7141 cagtgagttc atcgtgttgt gcttaggagc tcgaatttcc ccgatcgttc aaacatttgg
     7201 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
     7261 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
     7321 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
     7381 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa
     7441 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     7501 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     7561 tcgcccttcc caacagttgc gcagcctgaa tggcgcccgc tcctttcgct ttcttccctt
     7621 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     7681 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
     7741 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     7801 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcgggctatt
     7861 cttttgattt ataagggatt ttgccgattt cggaaccacc atcaaacagg attttcgcct
     7921 gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg cggtgaaggg
     7981 caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accccagtac attaaaaacg
     8041 tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc
     8101 caccagccag ccaacagctc cccgaccggc agctcggcac aaaatcacca ctcgatacag
     8161 gcagcccatc agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc
     8221 tcatgttacc gatgctattc ggaagaacgg caactaagct gccgggtttg aaacacggat
     8281 gatctcgcgg agggtagcat gttgattgta acgatgacag agcgttgctg cctgtgatca
     8341 aatatcatct ccctcgcaga gatccgaatt atcagccttc ttattcattt ctcgcttaac
     8401 cgtgacaggc tgtcgatctt gagaactatg ccgacataat aggaaatcgc tggataaagc
     8461 cgctgaggaa gctgagtggc gctatttctt tagaagtgaa cgttgacgat atcaactccc
     8521 ctatccattg ctcaccgaat ggtacaggtc ggggacccga agttccgact gtcggcctga
     8581 tgcatccccg gctgatcgac cccagatctg gggctgagaa agcccagtaa ggaaacaact
     8641 gtaggttcga gtcgcgagat cccccggaac caaaggaagt aggttaaacc cgctccgatc
     8701 aggccgagcc acgccaggcc gagaacattg gttcctgtag gcatcgggat tggcggatca
     8761 aacactaaag ctactggaac gagcagaagt cctccggccg ccagttgcca ggcggtaaag
     8821 gtgagcagag gcacgggagg ttgccacttg cgggtcagca cggttccgaa cgccatggaa
     8881 accgcccccg ccaggcccgc tgcgacgccg acaggatcta gcgctgcgtt tggtgtcaac
     8941 accaacagcg ccacgcccgc agttccgcaa atagccccca ggaccgccat caatcgtatc
     9001 gggctaccta gcagagcggc agagatgaac acgaccatca gcggctgcac agcgcctacc
     9061 gtcgccgcga ccccgcccgg caggcggtag accgaaataa acaacaagct ccagaatagc
     9121 gaaatattaa gtgcgccgag gatgaagatg cgcatccacc agattcccgt tggaatctgt
     9181 cggacgatca tcacgagcaa taaacccgcc ggcaacgccc gcagcagcat accggcgacc
     9241 cctcggcctc gctgttcggg ctccacgaaa acgccggaca gatgcgcctt gtgagcgtcc
     9301 ttggggccgt cctcctgttt gaagaccgac agcccaatga tctcgccgtc gatgtaggcg
     9361 ccgaatgcca cggcatctcg caaccgttca gcgaacgcct ccatgggctt tttctcctcg
     9421 tgctcgtaaa cggacccgaa catctctgga gctttcttca gggccgacaa tcggatctcg
     9481 cggaaatcct gcacgtcggc cgctccaagc cgtcgaatct gagccttaat cacaattgtc
     9541 aattttaatc ctctgtttat cggcagttcg tagagcgcgc cgtgcgtccc gagcgatact
     9601 gagcgaagca agtgcgtcga gcagtgcccg cttgttcctg aaatgccagt aaagcgctgg
     9661 ctgctgaacc cccagccgga actgacccca caaggcccta gcgtttgcaa tgcaccaggt
     9721 catcattgac ccaggcgtgt tccaccaggc cgctgcctcg caactcttcg caggcttcgc
     9781 cgacctgctc gcgccacttc ttcacgcggg tggaatccga tccgcacatg aggcggaagg
     9841 tttccagctt gagcgggtac ggctcccggt gcgagctgaa atagtcgaac atccgtcggg
     9901 ccgtcggcga cagcttgcgg tacttctccc atatgaattt cgtgtagtgg tcgccagcaa
     9961 acagcacgac gatttcctcg tcgatcagga cctggcaacg ggacgttttc ttgccacggt
    10021 ccaggacgcg gaagcggtgc agcagcgaca ccgattccag gtgcccaacg cggtcggacg
    10081 tgaagcccat cgccgtcgcc tgtaggcgcg acaggcattc ctcggccttc gtgtaatacc
    10141 ggccattgat cgaccagccc aggtcctggc aaagctcgta gaacgtgaag gtgatcggct
    10201 cgccgatagg ggtgcgcttc gcgtactcca acacctgctg ccacaccagt tcgtcatcgt
    10261 cggcccgcag ctcgacgccg gtgtaggtga tcttcacgtc cttgttgacg tggaaaatga
    10321 ccttgttttg cagcgcctcg cgcgggattt tcttgttgcg cgtggtgaac agggcagagc
    10381 gggccgtgtc gtttggcatc gctcgcatcg tgtccggcca cggcgcaata tcgaacaagg
    10441 aaagctgcat ttccttgatc tgctgcttcg tgtgtttcag caacgcggcc tgcttggcct
    10501 cgctgacctg ttttgccagg tcctcgccgg cggtttttcg cttcttggtc gtcatagttc
    10561 ctcgcgtgtc gatggtcatc gacttcgcca aacctgccgc ctcctgttcg agacgacgcg
    10621 aacgctccac ggcggccgat ggcgcgggca gggcaggggg agccagttgc acgctgtcgc
    10681 gctcgatctt ggccgtagct tgctggacca tcgagccgac ggactggaag gtttcgcggg
    10741 gcgcacgcat gacggtgcgg cttgcgatgg tttcggcatc ctcggcggaa aaccccgcgt
    10801 cgatcagttc ttgcctgtat gccttccggt caaacgtccg attcattcac cctccttgcg
    10861 ggattgcccc gactcacgcc ggggcaatgt gcccttattc ctgatttgac ccgcctggtg
    10921 ccttggtgtc cagataatcc accttatcgg caatgaagtc ggtcccgtag accgtctggc
    10981 cgtccttctc gtacttggta ttccgaatct tgccctgcac gaataccagc gaccccttgc
    11041 ccaaatactt gccgtgggcc tcggcctgag agccaaaaca cttgatgcgg aagaagtcgg
    11101 tgcgctcctg cttgtcgccg gcatcgttgc gccacatcta ggtactaaaa caattcatcc
    11161 agtaaaatat aatattttat tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat
    11221 agctcgacat actgttcttc cccgatatcc tccctgatcg accggacgca gaaggcaatg
    11281 tcataccact tgtccgccct gccgcttctc ccaagatcaa taaagccact tactttgcca
    11341 tctttcacaa agatgttgct gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg
    11401 ggcttttccg tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg agtgtcttct
    11461 tcccagtttt cgcaatccac atcggccaga tcgttattca gtaagtaatc caattcggct
    11521 aagcggctgt ctaagctatt cgtataggga caatccgata tgtcgatgga gtgaaagagc
    11581 ctgatgcact ccgcatacag ctcgataatc ttttcagggc tttgttcatc ttcatactct
    11641 tccgagcaaa ggacgccatc ggcctcactc atgagcagat tgctccagcc atcatgccgt
    11701 tcaaagtgca ggacctttgg aacaggcagc tttccttcca gccatagcat catgtccttt
    11761 tcccgttcca catcataggt ggtcccttta taccggctgt ccgtcatttt taaatatagg
    11821 ttttcatttt ctcccaccag cttatatacc ttagcaggag acattccttc cgtatctttt
    11881 acgcagcggt atttttcgat cagttttttc aattccggtg atattctcat tttagccatt
    11941 tattatttcc ttcctctttt ctacagtatt taaagatacc ccaagaagct aattataaca
    12001 agacgaactc caattcactg ttccttgcat tctaaaacct taaataccag aaaacagctt
    12061 tttcaaagtt gttttcaaag ttggcgtata acatagtatc gacggagccg attttgaaac
    12121 cacaattatg ggtgatgctg ccaacttact gatttagtgt atgatggtgt ttttgaggtg
    12181 ctccagtggc ttctgtgtct atcagctgtc cctcctgttc agctactgac ggggtggtgc
    12241 gtaacggcaa aagcaccgcc ggacatcagc gctatctctg ctctcactgc cgtaaaacat
    12301 ggcaactgca gttcacttac accgcttctc aacccggtac gcaccagaaa atcattgata
    12361 tggccatgaa tggcgttgga tgccgggcaa cagcccgcat tatgggcgtt ggcctcaaca
    12421 cgattttacg tcacttaaaa aactcaggcc gcagtcggta acctcgcgca tacagccggg
    12481 cagtgacgtc atcgtctgcg cggaaatgga cgaacagtgg ggctatgtcg gggctaaatc
    12541 gcgccagcgc tggctgtttt acgcgtatga cagtctccgg aagacggttg ttgcgcacgt
    12601 attcggtgaa cgcactatgg cgacgctggg gcgtcttatg agcctgctgt caccctttga
    12661 cgtggtgata tggatgacgg atggctggcc gctgtatgaa tcccgcctga agggaaagct
    12721 gcacgtaatc agcaagcgat atacgcagcg aattgagcgg cataacctga atctgaggca
    12781 gcacctggca cggctgggac ggaagtcgct gtcgttctca aaatcggtgg agctgcatga
    12841 caaagtcatc gggcattatc tgaacataaa acactatcaa taagttggag tcattaccca
    12901 attatgatag aatttacaag ctataaggtt attgtcctgg gtttcaagca ttagtccatg
    12961 caagttttta tgctttgccc attctataga tatattgata agcgcgctgc ctatgccttg
    13021 ccccctgaaa tccttacata cggcgatatc ttctatataa aagatatatt atcttatcag
    13081 tattgtcaat atattcaagg caatctgcct cctcatcctc ttcatcctct tcgtcttggt
    13141 agctttttaa atatggcgct tcatagagta attctgtaaa ggtccaattc tcgttttcat
    13201 acctcggtat aatcttacct atcacctcaa atggttcgct gggtttatcg cacccccgaa
    13261 cacgagcacg gcacccgcga ccactatgcc aagaatgccc aaggtaaaaa ttgccggccc
    13321 cgccatgaag tccgtgaatg ccccgacggc cgaagtgaag ggcaggccgc cacccaggcc
    13381 gccgccctca ctgcccggca cctggtcgct gaatgtcgat gccagcacct gcggcacgtc
    13441 aatgcttccg ggcgtcgcgc tcgggctgat cgcccatccc gttactgccc cgatcccggc
    13501 aatggcaagg actgccagcg ctgccatttt tggggtgagg ccgttcgcgg ccgaggggcg
    13561 cagcccctgg ggggatggga ggcccgcgtt agcgggccgg gagggttcga gaaggggggg
    13621 cacccccctt cggcgtgcgc ggtcacgcgc acagggcgca gccctggtta aaaacaaggt
    13681 ttataaatat tggtttaaaa gcaggttaaa agacaggtta gcggtggccg aaaaacgggc
    13741 ggaaaccctt gcaaatgctg gattttctgc ctgtggacag cccctcaaat gtcaataggt
    13801 gcgcccctca tctgtcagca ctctgcccct caagtgtcaa ggatcgcgcc cctcatctgt
    13861 cagtagtcgc gcccctcaag tgtcaatacc gcagggcact tatccccagg cttgtccaca
    13921 tcatctgtgg gaaactcgcg taaaatcagg cgttttcgcc gatttgcgag gctggccagc
    13981 tccacgtcgc cggccgaaat cgagcctgcc cctcatctgt caacgccgcg ccgggtgagt
    14041 cggcccctca agtgtcaacg tccgcccctc atctgtcagt gagggccaag ttttccgcga
    14101 ggtatccaca acgccggcgg ccgcggtgtc tcgcacacgg cttcgacggc gtttctggcg
    14161 cgtttgcagg gccatagacg gccgccagcc cagcggcgag ggcaaccagc ccgg
//
