LOCUS       62056_3890       13917 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..13917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(148..516)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(549..658)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(1342..1989)
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    complement(2454..2478)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2634..2817
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     rep_origin      complement(3224..3927)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     terminator      4022..4274
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4823..4844
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4859..4889
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     CDS             5004..6812
                     /codon_start=1
                     /label=GUS
                     /note="beta-glucuronidase"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     misc_feature    complement(7781..7805)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(9404..10549)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(10851..11642)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  11834..12601
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      complement(13302..13912)
                     /direction=LEFT
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
ORIGIN
        1 tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
     2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
     3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
     3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
     3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
     4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
     4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
     4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
     4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
     4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
     4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
     4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
     4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
     4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4921 caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc gactctagag
     4981 gatccccggg tggtcagtcc cttatgttac gtcctgtaga aaccccaacc cgtgaaatca
     5041 aaaaactcga cggcctgtgg gcattcagtc tggatcgcga aaactgtgga attgatcagc
     5101 gttggtggga aagcgcgtta caagaaagcc gggcaattgc tgtgccaggc agttttaacg
     5161 atcagttcgc cgatgcagat attcgtaatt atgcgggcaa cgtctggtat cagcgcgaag
     5221 tctttatacc gaaaggttgg gcaggccagc gtatcgtgct gcgtttcgat gcggtcactc
     5281 attacggcaa agtgtgggtc aataatcagg aagtgatgga gcatcagggc ggctatacgc
     5341 catttgaagc cgatgtcacg ccgtatgtta ttgccgggaa aagtgtacgt atcaccgttt
     5401 gtgtgaacaa cgaactgaac tggcagacta tcccgccggg aatggtgatt accgacgaaa
     5461 acggcaagaa aaagcagtct tacttccatg atttctttaa ctatgccgga atccatcgca
     5521 gcgtaatgct ctacaccacg ccgaacacct gggtggacga tatcaccgtg gtgacgcatg
     5581 tcgcgcaaga ctgtaaccac gcgtctgttg actggcaggt ggtggccaat ggtgatgtca
     5641 gcgttgaact gcgtgatgcg gatcaacagg tggttgcaac tggacaaggc actagcggga
     5701 ctttgcaagt ggtgaatccg cacctctggc aaccgggtga aggttatctc tatgaactgt
     5761 gcgtcacagc caaaagccag acagagtgtg atatctaccc gcttcgcgtc ggcatccggt
     5821 cagtggcagt gaagggcgaa cagttcctga ttaaccacaa accgttctac tttactggct
     5881 ttggtcgtca tgaagatgcg gacttgcgtg gcaaaggatt cgataacgtg ctgatggtgc
     5941 acgaccacgc attaatggac tggattgggg ccaactccta ccgtacctcg cattaccctt
     6001 acgctgaaga gatgctcgac tgggcagatg aacatggcat cgtggtgatt gatgaaactg
     6061 ctgctgtcgg ctttaacctc tctttaggca ttggtttcga agcgggcaac aagccgaaag
     6121 aactgtacag cgaagaggca gtcaacgggg aaactcagca agcgcactta caggcgatta
     6181 aagagctgat agcgcgtgac aaaaaccacc caagcgtggt gatgtggagt attgccaacg
     6241 aaccggatac ccgtccgcaa ggtgcacggg aatatttcgc gccactggcg gaagcaacgc
     6301 gtaaactcga cccgacgcgt ccgatcacct gcgtcaatgt aatgttctgc gacgctcaca
     6361 ccgataccat cagcgatctc tttgatgtgc tgtgcctgaa ccgttattac ggatggtatg
     6421 tccaaagcgg cgatttggaa acggcagaga aggtactgga aaaagaactt ctggcctggc
     6481 aggagaaact gcatcagccg attatcatca ccgaatacgg cgtggatacg ttagccgggc
     6541 tgcactcaat gtacaccgac atgtggagtg aagagtatca gtgtgcatgg ctggatatgt
     6601 atcaccgcgt ctttgatcgc gtcagcgccg tcgtcggtga acaggtatgg aatttcgccg
     6661 attttgcgac ctcgcaaggc atattgcgcg ttggcggtaa caagaaaggg atcttcactc
     6721 gcgaccgcaa accgaagtcg gcggcttttc tgctgcaaaa acgctggact ggcatgaact
     6781 tcggtgaaaa accgcagcag ggaggcaaac aatgaatcaa caactctcct ggcgcaccat
     6841 cgtcggctac agcctcggga attgctaccg agctcgaatt tccccgatcg ttcaaacatt
     6901 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa
     6961 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg
     7021 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa
     7081 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg
     7141 gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     7201 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     7261 cgatcgccct tcccaacagt tgcgcagcct gaatggcgcc cgctcctttc gctttcttcc
     7321 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     7381 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
     7441 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     7501 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcgggct
     7561 attcttttga tttataaggg attttgccga tttcggaacc accatcaaac aggattttcg
     7621 cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa
     7681 gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccccag tacattaaaa
     7741 acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc
     7801 tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata
     7861 caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg cggcagactt
     7921 tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg
     7981 gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg ctgcctgtga
     8041 tcaaatatca tctccctcgc agagatccga attatcagcc ttcttattca tttctcgctt
     8101 aaccgtgaca ggctgtcgat cttgagaact atgccgacat aataggaaat cgctggataa
     8161 agccgctgag gaagctgagt ggcgctattt ctttagaagt gaacgttgac gatatcaact
     8221 cccctatcca ttgctcaccg aatggtacag gtcggggacc cgaagttccg actgtcggcc
     8281 tgatgcatcc ccggctgatc gaccccagat ctggggctga gaaagcccag taaggaaaca
     8341 actgtaggtt cgagtcgcga gatcccccgg aaccaaagga agtaggttaa acccgctccg
     8401 atcaggccga gccacgccag gccgagaaca ttggttcctg taggcatcgg gattggcgga
     8461 tcaaacacta aagctactgg aacgagcaga agtcctccgg ccgccagttg ccaggcggta
     8521 aaggtgagca gaggcacggg aggttgccac ttgcgggtca gcacggttcc gaacgccatg
     8581 gaaaccgccc ccgccaggcc cgctgcgacg ccgacaggat ctagcgctgc gtttggtgtc
     8641 aacaccaaca gcgccacgcc cgcagttccg caaatagccc ccaggaccgc catcaatcgt
     8701 atcgggctac ctagcagagc ggcagagatg aacacgacca tcagcggctg cacagcgcct
     8761 accgtcgccg cgaccccgcc cggcaggcgg tagaccgaaa taaacaacaa gctccagaat
     8821 agcgaaatat taagtgcgcc gaggatgaag atgcgcatcc accagattcc cgttggaatc
     8881 tgtcggacga tcatcacgag caataaaccc gccggcaacg cccgcagcag cataccggcg
     8941 acccctcggc ctcgctgttc gggctccacg aaaacgccgg acagatgcgc cttgtgagcg
     9001 tccttggggc cgtcctcctg tttgaagacc gacagcccaa tgatctcgcc gtcgatgtag
     9061 gcgccgaatg ccacggcatc tcgcaaccgt tcagcgaacg cctccatggg ctttttctcc
     9121 tcgtgctcgt aaacggaccc gaacatctct ggagctttct tcagggccga caatcggatc
     9181 tcgcggaaat cctgcacgtc ggccgctcca agccgtcgaa tctgagcctt aatcacaatt
     9241 gtcaatttta atcctctgtt tatcggcagt tcgtagagcg cgccgtgcgt cccgagcgat
     9301 actgagcgaa gcaagtgcgt cgagcagtgc ccgcttgttc ctgaaatgcc agtaaagcgc
     9361 tggctgctga acccccagcc ggaactgacc ccacaaggcc ctagcgtttg caatgcacca
     9421 ggtcatcatt gacccaggcg tgttccacca ggccgctgcc tcgcaactct tcgcaggctt
     9481 cgccgacctg ctcgcgccac ttcttcacgc gggtggaatc cgatccgcac atgaggcgga
     9541 aggtttccag cttgagcggg tacggctccc ggtgcgagct gaaatagtcg aacatccgtc
     9601 gggccgtcgg cgacagcttg cggtacttct cccatatgaa tttcgtgtag tggtcgccag
     9661 caaacagcac gacgatttcc tcgtcgatca ggacctggca acgggacgtt ttcttgccac
     9721 ggtccaggac gcggaagcgg tgcagcagcg acaccgattc caggtgccca acgcggtcgg
     9781 acgtgaagcc catcgccgtc gcctgtaggc gcgacaggca ttcctcggcc ttcgtgtaat
     9841 accggccatt gatcgaccag cccaggtcct ggcaaagctc gtagaacgtg aaggtgatcg
     9901 gctcgccgat aggggtgcgc ttcgcgtact ccaacacctg ctgccacacc agttcgtcat
     9961 cgtcggcccg cagctcgacg ccggtgtagg tgatcttcac gtccttgttg acgtggaaaa
    10021 tgaccttgtt ttgcagcgcc tcgcgcggga ttttcttgtt gcgcgtggtg aacagggcag
    10081 agcgggccgt gtcgtttggc atcgctcgca tcgtgtccgg ccacggcgca atatcgaaca
    10141 aggaaagctg catttccttg atctgctgct tcgtgtgttt cagcaacgcg gcctgcttgg
    10201 cctcgctgac ctgttttgcc aggtcctcgc cggcggtttt tcgcttcttg gtcgtcatag
    10261 ttcctcgcgt gtcgatggtc atcgacttcg ccaaacctgc cgcctcctgt tcgagacgac
    10321 gcgaacgctc cacggcggcc gatggcgcgg gcagggcagg gggagccagt tgcacgctgt
    10381 cgcgctcgat cttggccgta gcttgctgga ccatcgagcc gacggactgg aaggtttcgc
    10441 ggggcgcacg catgacggtg cggcttgcga tggtttcggc atcctcggcg gaaaaccccg
    10501 cgtcgatcag ttcttgcctg tatgccttcc ggtcaaacgt ccgattcatt caccctcctt
    10561 gcgggattgc cccgactcac gccggggcaa tgtgccctta ttcctgattt gacccgcctg
    10621 gtgccttggt gtccagataa tccaccttat cggcaatgaa gtcggtcccg tagaccgtct
    10681 ggccgtcctt ctcgtacttg gtattccgaa tcttgccctg cacgaatacc agcgacccct
    10741 tgcccaaata cttgccgtgg gcctcggcct gagagccaaa acacttgatg cggaagaagt
    10801 cggtgcgctc ctgcttgtcg ccggcatcgt tgcgccacat ctaggtacta aaacaattca
    10861 tccagtaaaa tataatattt tattttctcc caatcaggct tgatccccag taagtcaaaa
    10921 aatagctcga catactgttc ttccccgata tcctccctga tcgaccggac gcagaaggca
    10981 atgtcatacc acttgtccgc cctgccgctt ctcccaagat caataaagcc acttactttg
    11041 ccatctttca caaagatgtt gctgtctccc aggtcgccgt gggaaaagac aagttcctct
    11101 tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa tggagtgtct
    11161 tcttcccagt tttcgcaatc cacatcggcc agatcgttat tcagtaagta atccaattcg
    11221 gctaagcggc tgtctaagct attcgtatag ggacaatccg atatgtcgat ggagtgaaag
    11281 agcctgatgc actccgcata cagctcgata atcttttcag ggctttgttc atcttcatac
    11341 tcttccgagc aaaggacgcc atcggcctca ctcatgagca gattgctcca gccatcatgc
    11401 cgttcaaagt gcaggacctt tggaacaggc agctttcctt ccagccatag catcatgtcc
    11461 ttttcccgtt ccacatcata ggtggtccct ttataccggc tgtccgtcat ttttaaatat
    11521 aggttttcat tttctcccac cagcttatat accttagcag gagacattcc ttccgtatct
    11581 tttacgcagc ggtatttttc gatcagtttt ttcaattccg gtgatattct cattttagcc
    11641 atttattatt tccttcctct tttctacagt atttaaagat accccaagaa gctaattata
    11701 acaagacgaa ctccaattca ctgttccttg cattctaaaa ccttaaatac cagaaaacag
    11761 ctttttcaaa gttgttttca aagttggcgt ataacatagt atcgacggag ccgattttga
    11821 aaccacaatt atgggtgatg ctgccaactt actgatttag tgtatgatgg tgtttttgag
    11881 gtgctccagt ggcttctgtg tctatcagct gtccctcctg ttcagctact gacggggtgg
    11941 tgcgtaacgg caaaagcacc gccggacatc agcgctatct ctgctctcac tgccgtaaaa
    12001 catggcaact gcagttcact tacaccgctt ctcaacccgg tacgcaccag aaaatcattg
    12061 atatggccat gaatggcgtt ggatgccggg caacagcccg cattatgggc gttggcctca
    12121 acacgatttt acgtcactta aaaaactcag gccgcagtcg gtaacctcgc gcatacagcc
    12181 gggcagtgac gtcatcgtct gcgcggaaat ggacgaacag tggggctatg tcggggctaa
    12241 atcgcgccag cgctggctgt tttacgcgta tgacagtctc cggaagacgg ttgttgcgca
    12301 cgtattcggt gaacgcacta tggcgacgct ggggcgtctt atgagcctgc tgtcaccctt
    12361 tgacgtggtg atatggatga cggatggctg gccgctgtat gaatcccgcc tgaagggaaa
    12421 gctgcacgta atcagcaagc gatatacgca gcgaattgag cggcataacc tgaatctgag
    12481 gcagcacctg gcacggctgg gacggaagtc gctgtcgttc tcaaaatcgg tggagctgca
    12541 tgacaaagtc atcgggcatt atctgaacat aaaacactat caataagttg gagtcattac
    12601 ccaattatga tagaatttac aagctataag gttattgtcc tgggtttcaa gcattagtcc
    12661 atgcaagttt ttatgctttg cccattctat agatatattg ataagcgcgc tgcctatgcc
    12721 ttgccccctg aaatccttac atacggcgat atcttctata taaaagatat attatcttat
    12781 cagtattgtc aatatattca aggcaatctg cctcctcatc ctcttcatcc tcttcgtctt
    12841 ggtagctttt taaatatggc gcttcataga gtaattctgt aaaggtccaa ttctcgtttt
    12901 catacctcgg tataatctta cctatcacct caaatggttc gctgggttta tcgcaccccc
    12961 gaacacgagc acggcacccg cgaccactat gccaagaatg cccaaggtaa aaattgccgg
    13021 ccccgccatg aagtccgtga atgccccgac ggccgaagtg aagggcaggc cgccacccag
    13081 gccgccgccc tcactgcccg gcacctggtc gctgaatgtc gatgccagca cctgcggcac
    13141 gtcaatgctt ccgggcgtcg cgctcgggct gatcgcccat cccgttactg ccccgatccc
    13201 ggcaatggca aggactgcca gcgctgccat ttttggggtg aggccgttcg cggccgaggg
    13261 gcgcagcccc tggggggatg ggaggcccgc gttagcgggc cgggagggtt cgagaagggg
    13321 gggcaccccc cttcggcgtg cgcggtcacg cgcacagggc gcagccctgg ttaaaaacaa
    13381 ggtttataaa tattggttta aaagcaggtt aaaagacagg ttagcggtgg ccgaaaaacg
    13441 ggcggaaacc cttgcaaatg ctggattttc tgcctgtgga cagcccctca aatgtcaata
    13501 ggtgcgcccc tcatctgtca gcactctgcc cctcaagtgt caaggatcgc gcccctcatc
    13561 tgtcagtagt cgcgcccctc aagtgtcaat accgcagggc acttatcccc aggcttgtcc
    13621 acatcatctg tgggaaactc gcgtaaaatc aggcgttttc gccgatttgc gaggctggcc
    13681 agctccacgt cgccggccga aatcgagcct gcccctcatc tgtcaacgcc gcgccgggtg
    13741 agtcggcccc tcaagtgtca acgtccgccc ctcatctgtc agtgagggcc aagttttccg
    13801 cgaggtatcc acaacgccgg cggccgcggt gtctcgcaca cggcttcgac ggcgtttctg
    13861 gcgcgtttgc agggccatag acggccgcca gcccagcggc gagggcaacc agcccgg
//
