LOCUS       62056_3880       15966 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15966)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..15966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(148..516)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(549..658)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(1342..1989)
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    complement(2454..2478)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2634..2817
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2841..3629
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     terminator      4022..4274
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4823..4844
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4859..4889
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     rep_origin      complement(5343..5967)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     intron          complement(6058..6826)
                     /label=PDK intron
                     /note="pyruvate orthophosphate dikinase intron from
                     Flaveria trinervia"
     CDS             7058..8866
                     /codon_start=1
                     /label=GUS
                     /note="beta-glucuronidase"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     misc_feature    complement(9830..9854)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11453..12598)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12900..13691)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13883..14650
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      complement(15351..15961)
                     /direction=LEFT
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
ORIGIN
        1 tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
     2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
     3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
     3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
     3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
     4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
     4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
     4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
     4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
     4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
     4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
     4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
     4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
     4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4921 caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc cccagattag
     4981 ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt acgcagcagg
     5041 tctcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc ttcccaagaa
     5101 ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat
     5161 atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc ttcacaaacc
     5221 aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat caaaggccat
     5281 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt
     5341 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca
     5401 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
     5461 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
     5521 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
     5581 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
     5641 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
     5701 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
     5761 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactctaga
     5821 gccaatttgt gacgaggttt tggttgaaca aatggctact tatttaatca aagactctaa
     5881 atttacggaa gaacttgctg ctgcaatgta ttccaagact ttagaagcta gtaatttttt
     5941 attcaaagta tgttaatgta ttagcactga ttcttcctta tggttcataa agaaataaaa
     6001 ttttaagtgt atttagtggt ggatccaagc ttatcgattt cgaacccagc ttcccaactg
     6061 taatcaatcc aaatgtaaga tcaatgataa cacaatgaca tgatctatca tgttaccttg
     6121 tttattcatg ttcgactaat tcatttaatt aatagtcaat ccatttagaa gttaataaaa
     6181 ctacaagtat tatttagaaa ttaataagaa tgttgattga aaaataatac tatataaaat
     6241 tgatagatct tgcgctttgt tatattagca ttagattatg ttttgttaca ttagattact
     6301 gtttctatta gtttgatatt atttgttact ttagcttgtt atttaatatt ttgtttattg
     6361 ataaattaca agcagattgg aatttctaac aaaatattta ttaactttta aactaaaata
     6421 tttagtaatg gtatagatat ttaattatat aataaactat taatcataaa aaaatattat
     6481 tttaatttat ttattcttat ttttactata gtattttatc attgatattt aattcatcaa
     6541 accagctaga attactatta tgattaaaac aaatattaat gctagtatat catcttacat
     6601 gttcgatcaa attcattaaa aataatatac ttactctcaa cttttatctt cttcgtctta
     6661 cacatcactt gtcatatttt tttacattac tatgttgttt atgtaaacaa tatatttata
     6721 aattattttt tcacaattat aacaactata ttattataat catactaatt aacatcactt
     6781 aactatttta tactaaaagg aaaaaagaaa ataattattt ccttaccaat tggggtacca
     6841 ccactaaata cacttaaaat tttatttctt tatgaaccat aaggaagaat cagtgctaat
     6901 acattaacat actttgaata aaaaattact agcttctaaa gtcttggaat acattgcagc
     6961 agcaagttct tccgtaaatt tagagtcttt gattaaataa gtagccattt gttcaaccaa
     7021 aacctcgtca caaattggcg agctcggtca gtcccttatg ttacgtcctg tagaaacccc
     7081 aacccgtgaa atcaaaaaac tcgacggcct gtgggcattc agtctggatc gcgaaaactg
     7141 tggaattgat cagcgttggt gggaaagcgc gttacaagaa agccgggcaa ttgctgtgcc
     7201 aggcagtttt aacgatcagt tcgccgatgc agatattcgt aattatgcgg gcaacgtctg
     7261 gtatcagcgc gaagtcttta taccgaaagg ttgggcaggc cagcgtatcg tgctgcgttt
     7321 cgatgcggtc actcattacg gcaaagtgtg ggtcaataat caggaagtga tggagcatca
     7381 gggcggctat acgccatttg aagccgatgt cacgccgtat gttattgccg ggaaaagtgt
     7441 acgtatcacc gtttgtgtga acaacgaact gaactggcag actatcccgc cgggaatggt
     7501 gattaccgac gaaaacggca agaaaaagca gtcttacttc catgatttct ttaactatgc
     7561 cggaatccat cgcagcgtaa tgctctacac cacgccgaac acctgggtgg acgatatcac
     7621 cgtggtgacg catgtcgcgc aagactgtaa ccacgcgtct gttgactggc aggtggtggc
     7681 caatggtgat gtcagcgttg aactgcgtga tgcggatcaa caggtggttg caactggaca
     7741 aggcactagc gggactttgc aagtggtgaa tccgcacctc tggcaaccgg gtgaaggtta
     7801 tctctatgaa ctgtgcgtca cagccaaaag ccagacagag tgtgatatct acccgcttcg
     7861 cgtcggcatc cggtcagtgg cagtgaaggg cgaacagttc ctgattaacc acaaaccgtt
     7921 ctactttact ggctttggtc gtcatgaaga tgcggacttg cgtggcaaag gattcgataa
     7981 cgtgctgatg gtgcacgacc acgcattaat ggactggatt ggggccaact cctaccgtac
     8041 ctcgcattac ccttacgctg aagagatgct cgactgggca gatgaacatg gcatcgtggt
     8101 gattgatgaa actgctgctg tcggctttaa cctctcttta ggcattggtt tcgaagcggg
     8161 caacaagccg aaagaactgt acagcgaaga ggcagtcaac ggggaaactc agcaagcgca
     8221 cttacaggcg attaaagagc tgatagcgcg tgacaaaaac cacccaagcg tggtgatgtg
     8281 gagtattgcc aacgaaccgg atacccgtcc gcaaggtgca cgggaatatt tcgcgccact
     8341 ggcggaagca acgcgtaaac tcgacccgac gcgtccgatc acctgcgtca atgtaatgtt
     8401 ctgcgacgct cacaccgata ccatcagcga tctctttgat gtgctgtgcc tgaaccgtta
     8461 ttacggatgg tatgtccaaa gcggcgattt ggaaacggca gagaaggtac tggaaaaaga
     8521 acttctggcc tggcaggaga aactgcatca gccgattatc atcaccgaat acggcgtgga
     8581 tacgttagcc gggctgcact caatgtacac cgacatgtgg agtgaagagt atcagtgtgc
     8641 atggctggat atgtatcacc gcgtctttga tcgcgtcagc gccgtcgtcg gtgaacaggt
     8701 atggaatttc gccgattttg cgacctcgca aggcatattg cgcgttggcg gtaacaagaa
     8761 agggatcttc actcgcgacc gcaaaccgaa gtcggcggct tttctgctgc aaaaacgctg
     8821 gactggcatg aacttcggtg aaaaaccgca gcagggaggc aaacaatgaa tcaacaactc
     8881 tcctggcgca ccatcgtcgg ctacagcctc gggaattgct acccgaattt ccccgatcgt
     8941 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt
     9001 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
     9061 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
     9121 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
     9181 ctagatcggg aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     9241 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     9301 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgccc gctcctttcg
     9361 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     9421 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     9481 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     9541 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     9601 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggaacca ccatcaaaca
     9661 ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca
     9721 ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccccagt
     9781 acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac
     9841 aatatatcct gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac
     9901 cactcgatac aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc
     9961 ggcagacttt gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt
    10021 tgaaacacgg atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc
    10081 tgcctgtgat caaatatcat ctccctcgca gagatccgaa ttatcagcct tcttattcat
    10141 ttctcgctta accgtgacag gctgtcgatc ttgagaacta tgccgacata ataggaaatc
    10201 gctggataaa gccgctgagg aagctgagtg gcgctatttc tttagaagtg aacgttgacg
    10261 atatcaactc ccctatccat tgctcaccga atggtacagg tcggggaccc gaagttccga
    10321 ctgtcggcct gatgcatccc cggctgatcg accccagatc tggggctgag aaagcccagt
    10381 aaggaaacaa ctgtaggttc gagtcgcgag atcccccgga accaaaggaa gtaggttaaa
    10441 cccgctccga tcaggccgag ccacgccagg ccgagaacat tggttcctgt aggcatcggg
    10501 attggcggat caaacactaa agctactgga acgagcagaa gtcctccggc cgccagttgc
    10561 caggcggtaa aggtgagcag aggcacggga ggttgccact tgcgggtcag cacggttccg
    10621 aacgccatgg aaaccgcccc cgccaggccc gctgcgacgc cgacaggatc tagcgctgcg
    10681 tttggtgtca acaccaacag cgccacgccc gcagttccgc aaatagcccc caggaccgcc
    10741 atcaatcgta tcgggctacc tagcagagcg gcagagatga acacgaccat cagcggctgc
    10801 acagcgccta ccgtcgccgc gaccccgccc ggcaggcggt agaccgaaat aaacaacaag
    10861 ctccagaata gcgaaatatt aagtgcgccg aggatgaaga tgcgcatcca ccagattccc
    10921 gttggaatct gtcggacgat catcacgagc aataaacccg ccggcaacgc ccgcagcagc
    10981 ataccggcga cccctcggcc tcgctgttcg ggctccacga aaacgccgga cagatgcgcc
    11041 ttgtgagcgt ccttggggcc gtcctcctgt ttgaagaccg acagcccaat gatctcgccg
    11101 tcgatgtagg cgccgaatgc cacggcatct cgcaaccgtt cagcgaacgc ctccatgggc
    11161 tttttctcct cgtgctcgta aacggacccg aacatctctg gagctttctt cagggccgac
    11221 aatcggatct cgcggaaatc ctgcacgtcg gccgctccaa gccgtcgaat ctgagcctta
    11281 atcacaattg tcaattttaa tcctctgttt atcggcagtt cgtagagcgc gccgtgcgtc
    11341 ccgagcgata ctgagcgaag caagtgcgtc gagcagtgcc cgcttgttcc tgaaatgcca
    11401 gtaaagcgct ggctgctgaa cccccagccg gaactgaccc cacaaggccc tagcgtttgc
    11461 aatgcaccag gtcatcattg acccaggcgt gttccaccag gccgctgcct cgcaactctt
    11521 cgcaggcttc gccgacctgc tcgcgccact tcttcacgcg ggtggaatcc gatccgcaca
    11581 tgaggcggaa ggtttccagc ttgagcgggt acggctcccg gtgcgagctg aaatagtcga
    11641 acatccgtcg ggccgtcggc gacagcttgc ggtacttctc ccatatgaat ttcgtgtagt
    11701 ggtcgccagc aaacagcacg acgatttcct cgtcgatcag gacctggcaa cgggacgttt
    11761 tcttgccacg gtccaggacg cggaagcggt gcagcagcga caccgattcc aggtgcccaa
    11821 cgcggtcgga cgtgaagccc atcgccgtcg cctgtaggcg cgacaggcat tcctcggcct
    11881 tcgtgtaata ccggccattg atcgaccagc ccaggtcctg gcaaagctcg tagaacgtga
    11941 aggtgatcgg ctcgccgata ggggtgcgct tcgcgtactc caacacctgc tgccacacca
    12001 gttcgtcatc gtcggcccgc agctcgacgc cggtgtaggt gatcttcacg tccttgttga
    12061 cgtggaaaat gaccttgttt tgcagcgcct cgcgcgggat tttcttgttg cgcgtggtga
    12121 acagggcaga gcgggccgtg tcgtttggca tcgctcgcat cgtgtccggc cacggcgcaa
    12181 tatcgaacaa ggaaagctgc atttccttga tctgctgctt cgtgtgtttc agcaacgcgg
    12241 cctgcttggc ctcgctgacc tgttttgcca ggtcctcgcc ggcggttttt cgcttcttgg
    12301 tcgtcatagt tcctcgcgtg tcgatggtca tcgacttcgc caaacctgcc gcctcctgtt
    12361 cgagacgacg cgaacgctcc acggcggccg atggcgcggg cagggcaggg ggagccagtt
    12421 gcacgctgtc gcgctcgatc ttggccgtag cttgctggac catcgagccg acggactgga
    12481 aggtttcgcg gggcgcacgc atgacggtgc ggcttgcgat ggtttcggca tcctcggcgg
    12541 aaaaccccgc gtcgatcagt tcttgcctgt atgccttccg gtcaaacgtc cgattcattc
    12601 accctccttg cgggattgcc ccgactcacg ccggggcaat gtgcccttat tcctgatttg
    12661 acccgcctgg tgccttggtg tccagataat ccaccttatc ggcaatgaag tcggtcccgt
    12721 agaccgtctg gccgtccttc tcgtacttgg tattccgaat cttgccctgc acgaatacca
    12781 gcgacccctt gcccaaatac ttgccgtggg cctcggcctg agagccaaaa cacttgatgc
    12841 ggaagaagtc ggtgcgctcc tgcttgtcgc cggcatcgtt gcgccacatc taggtactaa
    12901 aacaattcat ccagtaaaat ataatatttt attttctccc aatcaggctt gatccccagt
    12961 aagtcaaaaa atagctcgac atactgttct tccccgatat cctccctgat cgaccggacg
    13021 cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc tcccaagatc aataaagcca
    13081 cttactttgc catctttcac aaagatgttg ctgtctccca ggtcgccgtg ggaaaagaca
    13141 agttcctctt cgggcttttc cgtctttaaa aaatcataca gctcgcgcgg atctttaaat
    13201 ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca gatcgttatt cagtaagtaa
    13261 tccaattcgg ctaagcggct gtctaagcta ttcgtatagg gacaatccga tatgtcgatg
    13321 gagtgaaaga gcctgatgca ctccgcatac agctcgataa tcttttcagg gctttgttca
    13381 tcttcatact cttccgagca aaggacgcca tcggcctcac tcatgagcag attgctccag
    13441 ccatcatgcc gttcaaagtg caggaccttt ggaacaggca gctttccttc cagccatagc
    13501 atcatgtcct tttcccgttc cacatcatag gtggtccctt tataccggct gtccgtcatt
    13561 tttaaatata ggttttcatt ttctcccacc agcttatata ccttagcagg agacattcct
    13621 tccgtatctt ttacgcagcg gtatttttcg atcagttttt tcaattccgg tgatattctc
    13681 attttagcca tttattattt ccttcctctt ttctacagta tttaaagata ccccaagaag
    13741 ctaattataa caagacgaac tccaattcac tgttccttgc attctaaaac cttaaatacc
    13801 agaaaacagc tttttcaaag ttgttttcaa agttggcgta taacatagta tcgacggagc
    13861 cgattttgaa accacaatta tgggtgatgc tgccaactta ctgatttagt gtatgatggt
    13921 gtttttgagg tgctccagtg gcttctgtgt ctatcagctg tccctcctgt tcagctactg
    13981 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctatctc tgctctcact
    14041 gccgtaaaac atggcaactg cagttcactt acaccgcttc tcaacccggt acgcaccaga
    14101 aaatcattga tatggccatg aatggcgttg gatgccgggc aacagcccgc attatgggcg
    14161 ttggcctcaa cacgatttta cgtcacttaa aaaactcagg ccgcagtcgg taacctcgcg
    14221 catacagccg ggcagtgacg tcatcgtctg cgcggaaatg gacgaacagt ggggctatgt
    14281 cggggctaaa tcgcgccagc gctggctgtt ttacgcgtat gacagtctcc ggaagacggt
    14341 tgttgcgcac gtattcggtg aacgcactat ggcgacgctg gggcgtctta tgagcctgct
    14401 gtcacccttt gacgtggtga tatggatgac ggatggctgg ccgctgtatg aatcccgcct
    14461 gaagggaaag ctgcacgtaa tcagcaagcg atatacgcag cgaattgagc ggcataacct
    14521 gaatctgagg cagcacctgg cacggctggg acggaagtcg ctgtcgttct caaaatcggt
    14581 ggagctgcat gacaaagtca tcgggcatta tctgaacata aaacactatc aataagttgg
    14641 agtcattacc caattatgat agaatttaca agctataagg ttattgtcct gggtttcaag
    14701 cattagtcca tgcaagtttt tatgctttgc ccattctata gatatattga taagcgcgct
    14761 gcctatgcct tgccccctga aatccttaca tacggcgata tcttctatat aaaagatata
    14821 ttatcttatc agtattgtca atatattcaa ggcaatctgc ctcctcatcc tcttcatcct
    14881 cttcgtcttg gtagcttttt aaatatggcg cttcatagag taattctgta aaggtccaat
    14941 tctcgttttc atacctcggt ataatcttac ctatcacctc aaatggttcg ctgggtttat
    15001 cgcacccccg aacacgagca cggcacccgc gaccactatg ccaagaatgc ccaaggtaaa
    15061 aattgccggc cccgccatga agtccgtgaa tgccccgacg gccgaagtga agggcaggcc
    15121 gccacccagg ccgccgccct cactgcccgg cacctggtcg ctgaatgtcg atgccagcac
    15181 ctgcggcacg tcaatgcttc cgggcgtcgc gctcgggctg atcgcccatc ccgttactgc
    15241 cccgatcccg gcaatggcaa ggactgccag cgctgccatt tttggggtga ggccgttcgc
    15301 ggccgagggg cgcagcccct ggggggatgg gaggcccgcg ttagcgggcc gggagggttc
    15361 gagaaggggg ggcacccccc ttcggcgtgc gcggtcacgc gcacagggcg cagccctggt
    15421 taaaaacaag gtttataaat attggtttaa aagcaggtta aaagacaggt tagcggtggc
    15481 cgaaaaacgg gcggaaaccc ttgcaaatgc tggattttct gcctgtggac agcccctcaa
    15541 atgtcaatag gtgcgcccct catctgtcag cactctgccc ctcaagtgtc aaggatcgcg
    15601 cccctcatct gtcagtagtc gcgcccctca agtgtcaata ccgcagggca cttatcccca
    15661 ggcttgtcca catcatctgt gggaaactcg cgtaaaatca ggcgttttcg ccgatttgcg
    15721 aggctggcca gctccacgtc gccggccgaa atcgagcctg cccctcatct gtcaacgccg
    15781 cgccgggtga gtcggcccct caagtgtcaa cgtccgcccc tcatctgtca gtgagggcca
    15841 agttttccgc gaggtatcca caacgccggc ggccgcggtg tctcgcacac ggcttcgacg
    15901 gcgtttctgg cgcgtttgca gggccataga cggccgccag cccagcggcg agggcaacca
    15961 gcccgg
//
