LOCUS       pBI-CMV2.        3822 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bidirectional promoter vector for expressing a gene together with 
            the fluorescent protein AcGFP1.
ACCESSION   .
VERSION     .
KEYWORDS    pBI-CMV2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     The gene inserted into the MCS should contain start and stop codons.
FEATURES             Location/Qualifiers
     source          1..3822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        67..370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    602..663
                     /label=MCS 1
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    777..858
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1040..1628)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1802..2659)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2660..2764)
                     /label=AmpR promoter
     polyA_signal    complement(2797..2878)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(3020..3736)
                     /label=AcGFP1
                     /note="Aequorea coerulescens GFP"
     promoter        complement(3777..3814)
                     /note="minimal CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
ORIGIN
        1 ctcgagtagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      601 gggatcctct agtcagctga cgcgtgctag cgcggccgca tcgataagct tgtcgacgat
      661 atctccagag gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
      721 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
      781 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
      841 aagcattttt ttcactgccc cgagcttcct cgctcactga ctcgctgcgc tcggtcgttc
      901 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      961 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     1021 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     1081 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1141 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1201 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1261 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1321 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     1381 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1441 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     1501 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     1561 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1621 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1681 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     1741 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     1801 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1861 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     1921 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     1981 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     2041 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     2101 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     2161 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     2221 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     2281 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     2341 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     2401 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
     2461 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     2521 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     2581 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     2641 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     2701 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     2761 cgcgcacatt tccccgaaaa gtgccacctg acgtcggcag tgaaaaaaat gctttatttg
     2821 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     2881 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     2941 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcctctag actgcagcct
     3001 caggagatct gggccctcac ttgtacagct catccatgcc gtgggtgatg gcggcggcgg
     3061 tcacgaagcc gaagtagatc atgtgatcgc gcttctcgtt ggggtccttg gacagggcgc
     3121 tctgggtgga caggtagtgg ttatcgggca gcagcacagg gccatcgccg atgggggtat
     3181 tctgctggta gtggtcggcc agctgcacgc tgccatcctc gatgttgtgg cggatcttga
     3241 agttcacctt gatgccattc ttggccttgt cggtcatgat gtacacattg tgggcgttgt
     3301 agttgtactc catcttattg cccaggatgt tgccatcctc cttgaaatcg gtgccggtca
     3361 gctcgatgcg attcaccagg gtatcgccct cgaacttcac ctcggcgcgc gacttgtagt
     3421 tgccgtcatc ctcgaagaag atggtgcgct cctggatgta gccctcaggc atggcgctct
     3481 tgaagaagtc gtgctgcttc atgtgatcgg ggtagcgtga gaagcactgc acgccgtagc
     3541 tcagggtggt caccagggtg ggccagggca caggcagctt gccggtggtg cagatgaact
     3601 tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccgctcacg ctgaacttgt
     3661 ggccattcac atcgccattc agctcgatca ggatgggcac gatgccggtg aacagctcgg
     3721 cgcccttgct caccatggtg gcgaattctc caggcgatct gacggttcac taaacgagct
     3781 ctgcttatat aggcctccca ccgtacacgc cacctcgaca ta
//
