LOCUS       62056_3875        3816 bp DNA     circular SYN 17-NOV-2020
DEFINITION  Cloning vector pBI-CMV-EGFP, complete sequence.
ACCESSION   MT891343
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Frei T, Cella F, Tedeschi F, Gutierrez J, Stan GB, Khammash M, 
            Siciliano V.
  TITLE     Characterization and mitigation of gene expression burden in 
            mammalian cells
  JOURNAL   Nat Commun 11 (1), 4641 (2020)
  PUBMED    32934213
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Frei T, Cella F, Tedeschi F, Gutierrez J, Stan G-B., Khammash M, 
            Siciliano V.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-AUG-2020) Synthetic and Systems Biology for 
            Biomedicine, Italian Institute of Technology-IIT, Largo Barsanti e 
            Martteucci 53, Naples, Campania 80125, Italy
REFERENCE   3  (bases 1 to 3816)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun"; date: "2020"; volume: "11"; issue: "1"; pages: "4641"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-AUG-2020) Synthetic and Systems Biology for Biomedicine, Italian
            Institute of Technology-IIT, Largo Barsanti e Martteucci 53, Naples,
            Campania 80125, Italy"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(22..103)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(223..939)
                     /label=EGFP
                     /note="enhanced GFP"
     regulatory      complement(987..1055)
                     /label=minimal CMV promoter
                     /note="minimal CMV promoter"
                     /regulatory_class="promoter"
     enhancer        1122..1425
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1426..1629
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     polyA_signal    1818..1899
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2081..2669)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2843..3700)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3701..3805)
                     /label=AmpR promoter
ORIGIN
        1 gaaaagtgcc acctgacgtc ggcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
       61 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
      121 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
      181 ctacaaatgt ggtatggctg attatgatcc tctagactgc agcttgtaca gctcgtccat
      241 gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc
      301 gttggggtct ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac
      361 ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc
      421 ctcgatgttg tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat
      481 gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc
      541 ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt
      601 cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac
      661 gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
      721 gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcacgggcag
      781 cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc
      841 ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg
      901 caccaccccg gtgaacagct cctcgccctt gctcaccatg gtggcgaccg gtggatccgg
      961 gtcaccttta ccggtgaatt ctccaggcga tctgacggtt cactaaacga gctctgctta
     1021 tataggcctc ccaccgtaca cgccacctcg acatactcga gtagttatta atagtaatca
     1081 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     1141 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     1201 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     1261 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     1321 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     1381 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     1441 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     1501 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     1561 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     1621 agcagagctg gtttagtgaa ccgtcagatc cgctagggat cccttcgcta gcgcggccgc
     1681 atcgataagc ttgtcgacga tatctccaga ggatcataat cagccatacc acatttgtag
     1741 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     1801 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     1861 gcatcacaaa tttcacaaat aaagcatttt tttcactgcc ccgagcttcc tcgctcactg
     1921 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     1981 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     2041 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     2101 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     2161 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     2221 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     2281 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     2341 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     2401 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     2461 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     2521 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     2581 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     2641 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     2701 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     2761 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     2821 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     2881 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     2941 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     3001 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     3061 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     3121 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     3181 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     3241 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     3301 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     3361 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     3421 gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata
     3481 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     3541 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     3601 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     3661 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     3721 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     3781 aaaataaaca aataggggtt ccgcgcacat ttcccc
//
