LOCUS       V009205                 3856 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009205
VERSION     V009205
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Zeigler DR.
  TITLE     Sequence of pBGSC6, a simple integration vector for Gram-positive
            bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Zeigler DR.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2006) Bacillus Genetic Stock Center, The Ohio
            State University, 484 W. 12th Ave., Columbus, OH 43210, USA
REFERENCE   3  (bases 1 to 3856)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3856)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-APR-2006) Bacillus Genetic Stock Center, The Ohio State
            University, 484 W. 12th Ave., Columbus, OH 43210, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        142..172
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     204..220
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    complement(233..289)
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(290..306)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             951..1598
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             2055..2912
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3086..3674
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
      241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg
      301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
      361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
      421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
      481 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
      541 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
      601 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
      661 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
      721 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
      781 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
      841 aataaccctg ataaatgctt caataataaa aaaggattga ttctaatgaa gaaagcagac
      901 aagtaagcct cctaaattca ctttagataa aaatttagga ggcatatcaa atgaacttta
      961 ataaaattga tttagacaat tggaagagaa aagagatatt taatcattat ttgaaccaac
     1021 aaacgacttt tagtataacc acagaaattg atattagtgt tttataccga aacataaaac
     1081 aagaaggata taaattttac cctgcattta ttttcttagt gacaagggtg ataaactcaa
     1141 atacagcttt tagaactggt tacaatagcg acggagagtt aggttattgg gataagttag
     1201 agccacttta tacaattttt gatggtgtat ctaaaacatt ctctggtatt tggactcctg
     1261 taaagaatga cttcaaagag ttttatgatt tatacctttc tgatgtagag aaatataatg
     1321 gttcggggaa attgtttccc aaaacaccta tacctgaaaa tgctttttct ctttctatta
     1381 ttccatggac ttcatttact gggtttaact taaatatcaa taataatagt aattaccttc
     1441 tacccattat tacagcagga aaattcatta ataaaggtaa ttcaatatat ttaccgctat
     1501 ctttacaggt acatcattct gtttgtgatg gttatcatgc aggattgttt atgaactcta
     1561 ttcaggaatt gtcagatagg cctaatgact ggcttttata atatgagata atgccgactg
     1621 tactttttac agtcggtttt ctaatgtcac taacctgccc cgttagttga agaaggtttt
     1681 tatattacag ctccagatcc atatccttct ttttctgaac cgacttctcc tttttcgctt
     1741 ctttattcca attgctttat tgacgttgag cctcggaacc cttaacaatc ccaaaacttg
     1801 tcgaatggtc ggcttaatag ctcacgctat gccgacattc gtctgcaagt ttagttaagg
     1861 gttcttctca acgcacaata aattttctcg gcataaatgc gtggtctaat ttttattttt
     1921 aataaccttg atagcaaaaa atgccattcc aatacaaaac cacataccta taatcgataa
     1981 ccacataaca gtcataaaac cactcctttt taacaaactt tatcacaaga aatatttatt
     2041 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     2101 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     2161 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     2221 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     2281 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     2341 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     2401 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     2461 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     2521 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     2581 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     2641 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     2701 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     2761 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     2821 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     2881 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     2941 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     3001 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     3061 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3121 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3181 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     3241 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3301 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3361 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3421 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     3481 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3541 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3601 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3661 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     3721 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     3781 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     3841 gcgaggaagc ggaaga
//
