LOCUS       40924_6327        4336 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBGS18, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Curiao TIG., Coque T.
  TITLE     The complete sequence of the common cloning vector pBGS18
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Curiao TIG.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2014) Microbiology, University Hospital Ramn y 
            Cajal, Crtra Colmenar Viejo, Km 9, 100, Madrid, Madrid 28034, Spain
REFERENCE   3  (bases 1 to 4336)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4336)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-OCT-2014) Microbiology, University Hospital Ramn y Cajal, Crtra 
            Colmenar Viejo, Km 9, 100, Madrid, Madrid 28034, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Blast v. January 2013
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(354..783)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      1166..1754
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(1940..2082)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     protein_bind    2325..2346
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2361..2391
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2399..2415
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2423..2439
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2449..2505
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2509..2525)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(3086..3898)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 cgttgccgca actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
       61 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
      121 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
      181 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
      241 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
      301 aactgtcaga ccaagtttac tcatatatac tttagattga tttattttgt taaaattcgc
      361 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
      421 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
      481 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
      541 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
      601 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
      661 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
      721 agcggtcacg ctgcgcgtaa ccaccaccac ccgccgcgct taatgcgccg ctacagggcg
      781 cgtactatgg ttgctttgac gagcacgtat aacgtgcttt cctcgttaga atcagagcgg
      841 gagctaaaca ggaggccgat taaagggatt ttagacagga acggtacgcc agaatcctga
      901 gaagtgtttt tataatcagt gaggccaccg agtaaaagag tctgtccatc acgcaaatta
      961 accgttgtcg caatacttct ttgattagta ataacatcac ttgcctgagt agaagaactc
     1021 aaactatcgg ccttgctggt aatatccaga acaataaaag gatctaggtg aagatccttt
     1081 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     1141 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     1201 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     1261 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     1321 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     1381 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     1441 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     1501 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     1561 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     1621 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     1681 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     1741 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     1801 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     1861 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     1921 gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     1981 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     2041 atatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
     2101 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     2161 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
     2221 cagccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     2281 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     2341 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     2401 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacga attcgagctc
     2461 ggtacccggg gatcctctag agtcgacctg caggcatgca agcttggcac tggccgtcgt
     2521 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     2581 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     2641 gttgcgcagc ctgaatggcg aatgctgtaa tccgggcagc gcaacggaac attcatcagt
     2701 gtaaaaatgg aatcaataaa gccctgcgca gcgcgcaggg tcagcctgaa tacgcgttta
     2761 atgaccagca cagtcgtgat ggcaaggtca gaatagcgct gaggtctgcc tcgtgaagaa
     2821 ggtgttgctg actcatacca ggcctgaatc gccccatcat ccagccagaa agtgagggag
     2881 ccacggttga tgagagcttt gttgtaggtg gaccagttgg tgattttgaa cttttgcttt
     2941 gccacggaac ggtctgcgtt gtcgggaaga tgcgtgatct gatccttcaa ctcagcaaaa
     3001 gttcgattta ttcaacaaag ccgccgtccc gtcaagtcag cgtaatgctc tgccagtgtt
     3061 acaaccaatt aaccaattct gattagaaaa actcatcgag catcaaatga aactgcaatt
     3121 tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag
     3181 aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga
     3241 ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg
     3301 agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagttta tgcatttctt
     3361 tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca
     3421 aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag
     3481 gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa
     3541 tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttt ccggggatcg
     3601 cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag
     3661 gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc
     3721 tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcgataga
     3781 ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat
     3841 ccatgttgga atttaatcgc ggcctcgagc aagacgtttc ccgttgaata tggctcataa
     3901 caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat gatatatttt
     3961 tatcttgtgc aatgtaacat cagagatttt gagacaacgt ggctttgttg aataaatcga
     4021 acttttgctg agttgaagga tcagatcacg catcttcccg acaacgcaga ccgttccgtg
     4081 gcaaagcaaa agttcaaaat caccaactgg tccacctaca acaaagctct catcaaccgt
     4141 ggctccctca ctttctggct ggatgatggg gcgattcagg cctggtatga gtcagcaaca
     4201 ccttcttcac gaggcagacc tcagcgctat tctgaccttg ccatcacgac tgtgctggtc
     4261 attaaacgcg tattcaggct gaccctgcgc gctgcgcagg gctttattga ttccattttt
     4321 acactgatga atgttc
//
