LOCUS       40924_6317        9621 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBGEN02-hpt, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9621)
  AUTHORS   Kanoria S, Burma PK.
  TITLE     Cloning vectors for achieving high levels of transgene expression in
            plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9621)
  AUTHORS   Kanoria S, Burma PK.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2011) Genetics, University of Delhi, South Campus,
            Benito Juarez Road, New Delhi, New Delhi 110021, India
REFERENCE   3  (bases 1 to 9621)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9621)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-DEC-2011) Genetics, University of Delhi, South Campus, Benito 
            Juarez Road, New Delhi, New Delhi 110021, India"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9621
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1187..1813
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             2250..3314
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      3383..3577
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    3921..4063
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4249..4837)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    5814..5838
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    6152..6185
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6231..6236
                     /label=multiple cloning site 2 (between loxP)
                     /note="multiple cloning site 2 (between loxP)"
     promoter        6297..6641
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             6669..7691
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     regulatory      7710..7929
                     /label=35S
                     /note="35S"
                     /regulatory_class="polyA_signal_sequence"
     misc_feature    7991..8024
                     /label=loxP
                     /note="loxP"
     protein_bind    complement(7991..8024)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    8278..8543
                     /label=multiple cloning site 3 (outside loxP)
                     /note="multiple cloning site 3 (outside loxP)"
     regulatory      8616..8962
                     /label=35S
                     /note="35S"
                     /regulatory_class="promoter"
     promoter        8616..8962
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    8986..8999
                     /note="multiple cloning site 1 (between 35S promoter and
                     polyA)"
     misc_feature    9215..9318
                     /note="multiple cloning site 4 (for cloning another
                     transgene)"
     misc_feature    9485..9509
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
       61 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
      121 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
      181 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
      241 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
      301 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
      361 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
      421 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
      481 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
      541 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
      601 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
      661 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
      721 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
      781 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
      841 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
      901 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
      961 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
     1021 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
     1081 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
     1141 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
     1201 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
     1261 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
     1321 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
     1381 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
     1441 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
     1501 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
     1561 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
     1621 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
     1681 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
     1741 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
     1801 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
     1861 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
     1921 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
     1981 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
     2041 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
     2101 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
     2161 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
     2221 aacccccaag cccgaggaat cggcgtgacg gtcgcaaacc atccggcccg gtacaaatcg
     2281 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
     2341 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
     2401 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg
     2461 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
     2521 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
     2581 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
     2641 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
     2701 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
     2761 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
     2821 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
     2881 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
     2941 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
     3001 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
     3061 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
     3121 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
     3181 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
     3241 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
     3301 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
     3361 aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
     3421 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
     3481 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
     3541 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
     3601 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc
     3661 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac
     3721 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc
     3781 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     3841 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     3901 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
     3961 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     4021 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc
     4081 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     4141 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     4201 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     4261 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     4321 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     4381 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     4441 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     4501 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     4561 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     4621 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     4681 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     4741 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     4801 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     4861 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgcatgatat
     4921 atctcccaat ttgtgtaggg cttattatgc acgcttaaaa ataataaaag cagacttgac
     4981 ctgatagttt ggctgtgagc aattatgtgc ttagtgcatc taatcgcttg agttaacgcc
     5041 ggcgaagcgg cgtcggcttg aacgaatttc tagctagaca ttatttgccg actaccttgg
     5101 tgatctcgcc tttcacgtag tggacaaatt cttccaactg atctgcgcgc gaggccaagc
     5161 gatcttcttc ttgtccaaga taagcctgtc tagcttcaag tatgacgggc tgatactggg
     5221 ccggcaggcg ctccattgcc cagtcggcag cgacatcctt cggcgcgatt ttgccggtta
     5281 ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg ccagcccagt
     5341 cgggcggcga gttccatagc gttaaggttt catttagcgc ctcaaataga tcctgttcag
     5401 gaaccggatc aaagagttcc tccgccgctg gacctaccaa ggcaacgcta tgttctcttg
     5461 cttttgtcag caagatagcc agatcaatgt cgatcgtggc tggctcgaag atacctgcaa
     5521 gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg cttagctgga taacgccacg
     5581 gaatgatgtc gtcgtgcaca acaatggtga cttctacagc gcggagaatc tcgctctctc
     5641 caggggaagc cgaagtttcc aaaaggtcgt tgatcatagg atagagaatg aggatccaaa
     5701 gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg gctgcctgta
     5761 tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg
     5821 atatattgtg gtgtaaacac attgagccat agacaagtta ataacacatt gttgaggctt
     5881 tagtgtatgt aatcaatgat accattgcac tatatcttac aacacttcta tggacatgtg
     5941 aaggaggcca tgttggcctc cttgcacacc atacttgtgg tgtgtgcttg gatgaccatc
     6001 tatgtgcaat gagcatcact agagtgctcc atcatgacaa gtcattatcc ggtgatgaat
     6061 ggccatacta tcttaaggta gcaagatctt gtagtgaatg cattcatgat ggtcactctc
     6121 tcatgatagg agatggtatc ctcactatag cataacttcg tataatgtat gctatacgaa
     6181 gttatcacac tccttggtaa gaagcttgca tgcctgcagg tcaacatggt ggagcacgac
     6241 actctcgtct actccaagaa tatcaaagat acagtctcag aagaccagag ggctattgag
     6301 acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt
     6361 cacttcatcg aaaggacagt agaaaaggaa gatggcttct acaaatgcca tcattgcgat
     6421 aaaggaaagg ctatcgttca agatgcctct accgacagtg gtcccaaaga tggaccccca
     6481 cccacgagga acatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
     6541 tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac
     6601 ccttcctcta tataaggaag ttcatttcat ttggagagga cctcgagggc cccggggggc
     6661 aataagatat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
     6721 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
     6781 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
     6841 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
     6901 ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg
     6961 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctacaaccg gtcgcggagg
     7021 ctatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
     7081 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
     7141 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
     7201 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
     7261 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
     7321 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
     7381 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
     7441 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
     7501 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
     7561 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
     7621 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga
     7681 gggcaaagga atagagtaga tgccgaccgg agtccgcaaa aatcaccagt ctctctctac
     7741 aaatctatct ctctctattt ttctccagaa taatgtgtga gtagttccca gataagggaa
     7801 ttagggttct tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat
     7861 ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagtg
     7921 acctgcaggc atgcaagctt cttggagcta gatagatatc tctcatcatc ctaggtcata
     7981 acatagtggc ataacttcgt ataatgtatg ctatacgaag ttatgagcta tcatggatgg
     8041 actcaccatg tagtggagtg gtaactcttg gctcatgact ttggtccatg aaggtagagt
     8101 tgtagtatga gagaactatg catgaggaca tttgttgtgg agcatggtga atggcaagcc
     8161 tacaataaag ctatagcatg tctcatatca tgtgtcctac aatgttgaac ctccaagtag
     8221 caagagtgga gcaatcttgc accacttgtg tgcatatcta taccttgcat agaacaatat
     8281 tggtgcaaga ctagtcttgg acttaattaa ggatagacta tgagtgtatc ttgtagatgc
     8341 tccatgacac tcttgtattt gtaggccttg cacacttggt tatctacatt agctaccaag
     8401 tgctagtcaa taaagcaatg gaagtgtagc catgtattgt ctatcatcat acaatgaggt
     8461 attcaaggtg tggtgagaag tggagatggt cattagcatc atggcttgca aggtatgcca
     8521 ccatagagct cgacgtcgcg aaacatggtg gagcacgaca ctctcgtcta ctccaagaat
     8581 atcaaagata cagtctcaga agaccagagg gctattgaga cttttcaaca aagggtaata
     8641 tcgggaaacc tcctcggatt ccattgccca gttatctgtc acttcatcga aaggacagta
     8701 gaaaaggaag atggcttcta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
     8761 gatgcctcta ccgacagtgg tcccaaagat ggacccccac ccacgaggaa catcgtggaa
     8821 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgagatat ctccactgac
     8881 gtaagggatg acgcacaatc ccactatgcc ttcgcaagac ccttcctcta tataaggaag
     8941 ttcatttcat ttggagagga cacgctggaa ttctagtata ctaaaccatg gggcgcgccc
     9001 gggctacgta cgcaaatcac cagtctctct ctacaaatct atctctctct atttttctcc
     9061 agaataatgt gtgagtagtt cccagataag ggaattaggg ttcttatagg gtttcgctca
     9121 tgtgttgagc atataagaaa cccttagtat gtatttgtat tgtaaaatac ttctatcaat
     9181 aaaatttcta attcctaaaa ccaaaatcca gtcacgtgcc tcagcgagct cttgtcttgt
     9241 ctagacttca agtaagttta aactactcta tcaagtgatg ctaggcaatg gtgatgtcat
     9301 gtacctagat ttaaatgcaa ggctagatct agtgcatggc catcattgct taagtgctac
     9361 cggactcaat gacttgaaga gtgatgacaa ggcttgcttg catggccctt atggccttgc
     9421 catccataga tatggagtcc tcaagggtgc ttgtgataag aggcatgagc ctagtatgag
     9481 tgtatgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataacg
     9541 gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc
     9601 acagggttcc cctcgggatc a
//
