LOCUS       V009209                10549 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009209
VERSION     V009209
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10549)
  AUTHORS   Kanoria S, Burma PK.
  TITLE     Cloning vectors for achieving high levels of transgene expression in
            plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10549)
  AUTHORS   Kanoria S, Burma PK.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2011) Genetics, University of Delhi, South Campus,
            Benito Juarez Road, New Delhi, New Delhi 110021, India
REFERENCE   3  (bases 1 to 10549)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10549)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (13-DEC-2011) Genetics, University of Delhi, South Campus, Benito
            Juarez Road, New Delhi, New Delhi 110021, India"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10549
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1187..1813
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             2250..3314
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      3383..3577
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    3921..4063
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4249..4837)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    5814..5838
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    6152..6185
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        6595..6641
                     /label="minimal CaMV 35S promoter"
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             6657..8666
                     /gene="ALS"
                     /label="Acetolactate synthase, chloroplastic"
                     /note="Acetolactate synthase, chloroplastic from
                     Arabidopsis thaliana. Accession#: P17597"
     regulatory      8686..8902
                     /label="35S"
                     /note="35S"
                     /regulatory_class="polyA_signal_sequence"
     misc_feature    8919..8952
                     /label="loxP"
                     /note="loxP"
     protein_bind    complement(8919..8952)
                     /label="loxP"
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
     misc_feature    9206..9471
                     /label="multiple cloning site 2 (outside loxP)"
                     /note="multiple cloning site 2 (outside loxP)"
     promoter        9544..9890
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    9921..9941
                     /note="multiple cloning site 1 (between 35S promoter and
                     polyA)"
     misc_feature    10143..10240
                     /note="multiple cloning site 3 (for cloning another
                     transgene)"
     misc_feature    10413..10437
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
       61 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
      121 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
      181 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
      241 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
      301 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
      361 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
      421 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
      481 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
      541 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
      601 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
      661 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
      721 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
      781 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
      841 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
      901 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
      961 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
     1021 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
     1081 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
     1141 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
     1201 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
     1261 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
     1321 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
     1381 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
     1441 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
     1501 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
     1561 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
     1621 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
     1681 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
     1741 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
     1801 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
     1861 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
     1921 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
     1981 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
     2041 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
     2101 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
     2161 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
     2221 aacccccaag cccgaggaat cggcgtgacg gtcgcaaacc atccggcccg gtacaaatcg
     2281 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
     2341 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
     2401 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg
     2461 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
     2521 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
     2581 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
     2641 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
     2701 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
     2761 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
     2821 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
     2881 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
     2941 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
     3001 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
     3061 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
     3121 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
     3181 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
     3241 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
     3301 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
     3361 aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
     3421 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
     3481 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
     3541 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
     3601 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc
     3661 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac
     3721 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc
     3781 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     3841 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     3901 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
     3961 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     4021 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc
     4081 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     4141 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     4201 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     4261 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     4321 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     4381 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     4441 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     4501 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     4561 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     4621 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     4681 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     4741 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     4801 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     4861 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgcatgatat
     4921 atctcccaat ttgtgtaggg cttattatgc acgcttaaaa ataataaaag cagacttgac
     4981 ctgatagttt ggctgtgagc aattatgtgc ttagtgcatc taatcgcttg agttaacgcc
     5041 ggcgaagcgg cgtcggcttg aacgaatttc tagctagaca ttatttgccg actaccttgg
     5101 tgatctcgcc tttcacgtag tggacaaatt cttccaactg atctgcgcgc gaggccaagc
     5161 gatcttcttc ttgtccaaga taagcctgtc tagcttcaag tatgacgggc tgatactggg
     5221 ccggcaggcg ctccattgcc cagtcggcag cgacatcctt cggcgcgatt ttgccggtta
     5281 ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg ccagcccagt
     5341 cgggcggcga gttccatagc gttaaggttt catttagcgc ctcaaataga tcctgttcag
     5401 gaaccggatc aaagagttcc tccgccgctg gacctaccaa ggcaacgcta tgttctcttg
     5461 cttttgtcag caagatagcc agatcaatgt cgatcgtggc tggctcgaag atacctgcaa
     5521 gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg cttagctgga taacgccacg
     5581 gaatgatgtc gtcgtgcaca acaatggtga cttctacagc gcggagaatc tcgctctctc
     5641 caggggaagc cgaagtttcc aaaaggtcgt tgatcatagg atagagaatg aggatccaaa
     5701 gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg gctgcctgta
     5761 tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg
     5821 atatattgtg gtgtaaacac attgagccat agacaagtta ataacacatt gttgaggctt
     5881 tagtgtatgt aatcaatgat accattgcac tatatcttac aacacttcta tggacatgtg
     5941 aaggaggcca tgttggcctc cttgcacacc atacttgtgg tgtgtgcttg gatgaccatc
     6001 tatgtgcaat gagcatcact agagtgctcc atcatgacaa gtcattatcc ggtgatgaat
     6061 ggccatacta tcttaaggta gcaagatctt gtagtgaatg cattcatgat ggtcactctc
     6121 tcatgatagg agatggtatc ctcactatag cataacttcg tataatgtat gctatacgaa
     6181 gttatcacac tccttggtaa gcttggagct agatagatgg tcaacatggt ggagcacgac
     6241 actctcgtct actccaagaa tatcaaagat acagtctcag aagaccagag ggctattgag
     6301 acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt
     6361 cacttcatcg aaaggacagt agaaaaggaa gatggcttct acaaatgcca tcattgcgat
     6421 aaaggaaagg ctatcgttca agaatgcctc taccgacagt ggtcccaaag atggaccccc
     6481 acccacgagg aacatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
     6541 ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga
     6601 cccttcctct atataaggaa gttcatttca tttggagagg acctcgagtg gccaccatgg
     6661 cggcggcaac aacaacaaca acaacatctt cttcgatctc cttctccacc aaaccatctc
     6721 cttcctcctc caaatcacca ttaccaatct ccagattctc cctcccattc tccctaaacc
     6781 ccaacaaatc atcctcctcc tcccgccgcc gcggtatcaa atccagctct ccctcctcca
     6841 tctccgccgt gctcaacaca accaccaatg tcacaaccac tccctctcca accaaaccta
     6901 ccaaacccga aacattcatc tcccgattcg ctccagatca accccgcaaa ggcgctgata
     6961 tcctcgtcga agctttagaa cgtcaaggcg tagaaaccgt attcgcttac cctggaggtg
     7021 catcaatgga gattcaccaa gccttaaccc gctcttcctc aatccgtaac gtccttcctc
     7081 gtcacgaaca aggaggtgta ttcgcagcag aaggatacgc tcgatcctca ggtaaaccag
     7141 gtatctgtat agccacttca ggtcccggag ctacaaatct cgttagcgga ttagccgatg
     7201 cgttgttaga tagtgttcct cttgtagcaa tcacaggaca agtctctcgt cgtatgattg
     7261 gtacagatgc gtttcaagag actccgattg ttgaggtaac gcgttcgatt acgaagcata
     7321 actatcttgt gatggatgtt gaagatatcc ctaggattat tgaggaagct ttctttttag
     7381 ctacttctgg tagacctgga cctgttttgg ttgatgttcc taaagatatt caacaacagc
     7441 ttgcgattcc taattgggaa caggctatga gattacctgg ttatatgtct aggatgccta
     7501 aacctccgga agattctcat ttggagcaga ttgttaggtt gatttctgag tctaagaagc
     7561 ctgtgttgta tgttggtggt ggttgtttga attctagcga tgaattgggt aggtttgttg
     7621 agcttacggg gatccctgtt gcgagtacgt tgatggggct gggatcttat ccttgtgatg
     7681 atgagttgtc gttacatatg cttggaatgc atgggactgt gtatgcaaat tacgctgtgg
     7741 agcatagtga tttgttgttg gcgtttgggg taaggtttga tgatcgtgtc acgggtaagc
     7801 ttgaggcttt tgctagtagg gctaagattg ttcatattga tattgactcg gctgagattg
     7861 ggaagaataa gactcctcat gtgtctgtgt gtggtgatgt taagctggct ttgcaaggga
     7921 tgaataaggt tcttgagaac cgagcggagg agcttaagct tgattttgga gtttggagga
     7981 atgagttgaa cgtacagaaa cagaagtttc cgttgagctt taagacgttt ggggaagcta
     8041 ttcctccaca gtatgcgatt aaggtccttg atgagttgac tgatggaaaa gccataataa
     8101 gtactggtgt cgggcaacat caaatgtggg cggcgcagtt ctacaattac aagaaaccaa
     8161 ggcagtggct atcatcagga ggccttggag ctatgggatt tggacttcct gctgcgattg
     8221 gagcgtctgt tgctaaccct gatgcgatag ttgtggatat tgacggagat ggaagcttta
     8281 taatgaatgt gcaagagcta gccactattc gtgtagagaa tcttccagtg aaggtacttt
     8341 tattaaacaa ccagcatctt ggcatggtta tgcaatggga agatcggttc tacaaagcta
     8401 accgagctca cacatttctc ggggatccgg ctcaggagga cgagatattc ccgaacatgt
     8461 tgctgtttgc agcagcttgc gggattccag cggcgagggt gacaaagaaa gcagatctcc
     8521 gagaagctat tcagacaatg ctggatacac caggacctta cctgttggat gtgatttgtc
     8581 cgcaccaaga acatgtgttg ccgatgatcc cgaatggtgg cactttcaac gatgtcataa
     8641 cggaaggaga tggccggatt aaatactgat ctagagtccg caaatcacca gtctctctct
     8701 acaaatctat ctctctctat ttttctccag aataatgtgt gagtagttcc cagataaggg
     8761 aattagggtt cttatagggt ttcgctcatg tgttgagcat ataagaaacc cttagtatgt
     8821 atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac caaaatccag
     8881 tgaccatctc tcatcatcct aggtcataac atagtggcat aacttcgtat aatgtatgct
     8941 atacgaagtt atgagctatc atggatggac tcaccatgta gtggagtggt aactcttggc
     9001 tcatgacttt ggtccatgaa ggtagagttg tagtatgaga gaactatgca tgaggacatt
     9061 tgttgtggag catggtgaat ggcaagccta caataaagct atagcatgtc tcatatcatg
     9121 tgtcctacaa tgttgaacct ccaagtagca agagtggagc aatcttgcac cacttgtgtg
     9181 catatctata ccttgcatag aacaatattg gtgcaagact agtcttggac ttaattaagg
     9241 atagactatg agtgtatctt gtagatgctc catgacactc ttgtatttgt aggccttgca
     9301 cacttggtta tctacattag ctaccaagtg ctagtcaata aagcaatgga agtgtagcca
     9361 tgtattgtct atcatcatac aatgaggtat tcaaggtgtg gtgagaagtg gagatggtca
     9421 ttagcatcat ggcttgcaag gtatgccacc atagagctcg acgtcgcgaa acatggtgga
     9481 gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag accagagggc
     9541 tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagt
     9601 tatctgtcac ttcatcgaaa ggacagtaga aaaggaagat ggcttctaca aatgccatca
     9661 ttgcgataaa ggaaaggcta tcgttcaaga tgcctctacc gacagtggtc ccaaagatgg
     9721 acccccaccc acgaggaaca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca
     9781 agtggattga tgagatatct ccactgacgt aagggatgac gcacaatccc actatgcctt
     9841 cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaca cgctggaatt
     9901 ctagtatact aaaccatggg gcgcgcccgg gctacgtacg caaatcacca gtctctctct
     9961 acaaatctat ctctctctat ttttctccag aataatgtgt gagtagttcc cagataaggg
    10021 aattagggtt cttatagggt ttcgctcatg tgttgagcat ataagaaacc cttagtatgt
    10081 atttgtattg taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt
    10141 cacgtgcctc agcgagctct tgtcttgtct agacttcaag taagtttaaa ctactctatc
    10201 aagtgatgct aggcaatggt gatgtcatgt acctagattt aaatgcaagg ctagatctag
    10261 tgcatggcca tcattgctta agtgctaccg gactcaatga cttgaagagt gatgacaagg
    10321 cttgcttgca tggcccttat ggccttgcca tccatagata tggagtcctc aagggtgctt
    10381 gtgataagag gcatgagcct agtatgagtg tatgacagga tatattggcg ggtaaaccta
    10441 agagaaaaga gcgtttatta gaataacgga tatttaaaag ggcgtgaaaa ggtttatccg
    10501 ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggatca
//
