LOCUS       62056_3830        4102 bp DNA     circular SYN 09-DEC-2020
DEFINITION  Cloning vector pBGC, complete sequence.
ACCESSION   MT702881
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Alonso-del Valle A, Leon-Sampedro R, Rodriguez-Beltran J, DelaFuente
            J, Hernandez-Garcia M, Ruiz-Garbajosa P, Canton R, Pena-Miller R, 
            Millan AS.
  TITLE     The distribution of plasmid fitness effects explains plasmid 
            persistence in bacterial communities
  JOURNAL   bioRxiv (2020) In press
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Alonso-del Valle A.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUN-2020) Microbiology Service-Plasmid Biology and 
            Evolution Lab, Ramon y Cajal Health Research Institute (IRyCIS), 
            Ctra. Colmenar Viejo Km 9, 100, Madrid 28034, Spain
REFERENCE   3  (bases 1 to 4102)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            (2020) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-JUN-2020) Microbiology Service-Plasmid Biology and Evolution 
            Lab, Ramon y Cajal Health Research Institute (IRyCIS), Ctra. 
            Colmenar Viejo Km 9, 100, Madrid 28034, Spain"
FEATURES             Location/Qualifiers
     source          1..4102
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..106
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             107..763
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     rep_origin      827..1371
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     protein_bind    1742..1763
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1778..1808
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1816..1832
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1840..1856
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(1889..2764)
                     /codon_start=1
                     /label=araC
                     /note="L-arabinose regulatory protein"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        2791..3075
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             3133..3846
                     /codon_start=1
                     /label=GFP
                     /note="Aequorea victoria green fluorescent protein"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      4001..4028
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
ORIGIN
        1 cgttgatcgg cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg
       61 cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat
      121 cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt
      181 tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt
      241 aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg
      301 cctgatgaat gctcatccgg aatttcgtat ggcaatgaaa gacggtgagc tggtgatatg
      361 ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct
      421 ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc
      481 gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt
      541 ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa
      601 cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat
      661 gccgctggcg attcaggttc atcatgccgt ctgtgatggc ttccatgtcg gcagaatgct
      721 taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag
      781 ttattggtgc ctagaaatat tttatctgat taataagatg atcttcttga gatcgttttg
      841 gtctgcgcgt aatctcttgc tctgaaaacg aaaaaaccgc cttgcagggc ggtttttcga
      901 aggttctctg agctaccaac tctttgaacc gaggtaactg gcttggagga gcgcagtcac
      961 caaaacttgt cctttcagtt tagccttaac cggcgcatga cttcaagact aactcctcta
     1021 aatcaattac cagtggctgc tgccagtggt gcttttgcat gtctttccgg gttggactca
     1081 agacgatagt taccggataa ggcgcagcgg tcggactgaa cggggggttc gtgcatacag
     1141 tccagcttgg agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacaaacg
     1201 cggccataac agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag
     1261 ggagccgcca ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccaccactga
     1321 tttgagcgtc agatttcgtg atgcttgtca ggggggcgga gcctatggaa aaacggcttt
     1381 gccgcggccc tctcacttcc ctgttaagta tcttcctggc atcttccagg aaatctccgc
     1441 cccgttcgta agccatttcc gctcgccgca gtcgaacgac cgagcgtagc gagtcagtga
     1501 gcgaggaagc ggaatatatc ctgtatcaca tattctgctg acgcaccggt gcagcctttt
     1561 ttctcctgcc acatgaagca cttcactgac accctcatca gtgccaacat agtaagccag
     1621 tatatacact ccgctagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     1681 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     1741 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     1801 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     1861 attacgaatt cgagctcggt accaattatg acaacttgac ggctacatca ttcacttttt
     1921 cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg
     1981 agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg
     2041 tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc
     2101 taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct
     2161 gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag
     2221 cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc
     2281 gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt
     2341 gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg
     2401 ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg
     2461 cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt
     2521 agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc
     2581 gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca
     2641 ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac
     2701 ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag
     2761 ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac
     2821 attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta
     2881 ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg
     2941 tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg
     3001 ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct
     3061 ctactgtttc tccatacccg tttttttgga tggagtgaaa cgatggcgat tgcaattgga
     3121 attcccggcc gaatgagtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
     3181 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat
     3241 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca
     3301 tggccaacac ttgtcactac tttctcttat ggtgttcaat gcttttcaag atacccagat
     3361 catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga
     3421 actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt
     3481 gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt
     3541 cttggacaca aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa
     3601 caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt
     3661 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca
     3721 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac
     3781 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta
     3841 tacaaatagt ctagacagcg ggaatcctgc tctgcgaggc tggccggctg caggcatgca
     3901 agcttggcac tggccgtcgt ttgttagata tttatcctcg agcggcccgg agggtggcgg
     3961 gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat
     4021 ggcctttttg cgtttctaca aactcttttg tttatttttc taaatacatt caaatatgta
     4081 tccgctcatg agacaataac cc
//
