LOCUS       40924_6207        7522 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pBEVY-A, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7522)
  AUTHORS   Miller CA III, Martinat MA, Hyman LE.
  TITLE     Assessment of Aryl Hydrocarbon Receptor Complex Interactions Using 
            pBEVY Plasmids: Expression Vectors With Bi-directional Promoters For
            Saccharomyces cerevisiae
  JOURNAL   Nucleic Acids Res. (1998) In press
REFERENCE   2  (bases 1 to 7522)
  AUTHORS   Miller CA III.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUN-1998) Environmental Health Sciences, Tulane 
            University School of Public Health and Tropical Medicine, 1430 
            Tulane Ave., New Orleans, LA 70112, USA
REFERENCE   3  (bases 1 to 7522)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7522)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (1998) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-JUN-1998) Environmental Health Sciences, Tulane University 
            School of Public Health and Tropical Medicine, 1430 Tulane Ave., New
            Orleans, LA 70112, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7522
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        142..172
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(267..454)
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     promoter        complement(623..1268)
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate
                     dehydrogenase; also known as the TDH3 promoter"
     promoter        1280..1673
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     terminator      1712..2011
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 2 (ADH2) gene"
     rep_origin      complement(2036..3200)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(3485..5197)
                     /codon_start=1
                     /label=ADE2
                     /note="phosphoribosylaminoimidazole carboxylase, required
                     for adenine biosynthesis"
                     /translation="MDSRTVGILGGGQLGRMIVEAANRLNIKTVILDAENSPAKQISNS
                     NDHVNGSFSNPLDIEKLAEKCDVLTIEIEHVDVPTLKNLQVKHPKLKIYPSPETIRLIQ
                     DKYIQKEHLIKNGIAVTQSVPVEQASETSLLNVGRDLGFPFVLKSRTLAYDGRGNFVVK
                     NKEMIPEALEVLKDRPLYAEKWAPFTKELAVMIVRSVNGLVFSYPIVETIHKDNICDLC
                     YAPARVPDSVQLKAKLLAENAIKSFPGCGIFGVEMFYLETGELLINEIAPRPHNSGHYT
                     IDACVTSQFEAHLRSILDLPMPKNFTSFSTITTNAIMLNVLGDKHTKDKELETCERALA
                     TPGSSVYLYGKESRPNRKVGHINIIASSMAECEQRLNYITGRTDIPIKISVAQKLDLEA
                     MVKPLVGIIMGSDSDLPVMSAACAVLKDFGVPFEVTIVSAHRTPHRMSAYAISASKRGI
                     KTIIAGAGGAAHLPGMVAAMTPLPVIGVPVKGSCLDGVDSLHSIVQMPRGVPVATVAIN
                     NSTNAALLAVRLLGAYDSSYTTKMEQFLLKQEEEVLVKAQKLETVGYEAYLENK"
     promoter        complement(5198..5573)
                     /label=ADE2 promoter
     promoter        5616..5720
                     /label=AmpR promoter
     CDS             5721..6578
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6752..7340
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagctttt
      241 catagggtag gggaatttcg ggcatgccgg tagaggtgtg gtcaataaga gcgacctcat
      301 gctatacctg agaaagcaac ctgacctaca ggaaagagtt actcaagaac aagaattttc
      361 gttttaaaac ctaagagtca ctttaaaatt tgtatacact tatttttttt ataacttatt
      421 taataataaa aatcataaat cataagaaat tcgcttattt agaagtgtca acaacgtatc
      481 taccaacgat ttgacccttt tccatctttt cgtaaatttc tggcaaggta gacaagccga
      541 caaccttgat tggagacttg accaaacctc tggcgaagaa gtccaaagct tgcatgcctg
      601 caggtcgact ctagaggatc cgtcgaaact aagttcttgg tgttttaaaa ctaaaaaaaa
      661 agactaacta taaaagtaga atttaagaag tttaagaaat agatttacag aattacaatc
      721 aatacctacc gttctttata tacttattag tcaagtaggg gaataatttc agggaactgg
      781 tttcaacctt ttttttcagc tttttccaaa tcagagagag cagaaggtaa tagaaggtgt
      841 aagaaaatga gatagataca tgcgtgggtc aattgccttg tgtcatcatt tactccaggc
      901 aggttgcatc actccattga ggttgtgccc gttttttgcc tgtttgtgcc cctgttctct
      961 gtagttgcgc taagagaatg gacctatgaa ctgatggttg gtgaagaaaa caatattttg
     1021 gtgctgggat tctttttttt tctggatgcc agcttaaaaa gcgggctcca ttatatttag
     1081 tggatgccag gaataaactg ttcacccaga cacctacgat gttatatatt ctgtgtaacc
     1141 cgccccctat tttgggcatg tacgggttac agcagaatta aaaggctaat tttttgacta
     1201 aataaagtta ggaaaatcac tactattaat tatttacgta ttctttgaaa tggcagtatt
     1261 gataatgata aactcccgga ttcttttctt tttttttctt ttctctctcc cccgttgttg
     1321 tctcaccata tccgcaatga caaaaaaaat gatggaagac actaaaggaa aaaattaacg
     1381 acaaagacag caccaacaga tgtcgttgtt ccagagctga tgaggggtat cttcgaacac
     1441 acgaaacttt ttccttcctt cattcacgca cactactctc taatgagcaa cggtatacgg
     1501 ccttccttcc agttacttga atttgaaata aaaaaagttt gccgctttgc tatcaagtat
     1561 aaatagacct gcaattatta atcttttgtt tcctcgtcat tgttctcgtt ccctttcttc
     1621 cttgtttctt tttctgcaca atatttcaag ctataccaag catacaatca actccccggg
     1681 taccgagctc gaattcgaca cttctaaata agcggatctc ctatgccttc acgatttata
     1741 gtttccatta tcaagtatgc ctatattagt atatagcatc tttagatgac agtgttcgaa
     1801 gtttcacgaa taaaagataa tattctactt tttgctccca ccgcgtttgc tagcacgagt
     1861 gaacaccatc cctcgcctgt gagttgtacc cattcctcta aactgtagac atggtagctt
     1921 cagcagtgtt cgttatgtac ggcatcctcc aacaaacagt cggttatagt ttgtcctgct
     1981 cctctgaatc gtctccctcg atatttctca ttttccttcg catgccctcg agcggaacga
     2041 agcatctgtg cttcattttg tagaacaaaa atgcaacgcg agagcgctaa tttttcaaac
     2101 aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgaaagcgc tattttacca
     2161 acgaagaatc tgtgcttcat ttttgtaaaa caaaaatgca acgcgagagc gctaattttt
     2221 caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgag agcgctattt
     2281 taccaacaaa gaatctatac ttcttttttg ttctacaaaa atgcatcccg agagcgctat
     2341 ttttctaaca aagcatctta gattactttt tttctccttt gtgcgctcta taatgcagtc
     2401 tcttgataac tttttgcact gtaggtccgt taaggttaga agaaggctac tttggtgtct
     2461 attttctctt ccataaaaaa agcctgactc cacttcccgc gtttactgat tactagcgaa
     2521 gctgcgggtg cattttttca agataaaggc atccccgatt atattctata ccgatgtgga
     2581 ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga ttcttcattg gtcagaaaat
     2641 tatgaacggt ttcttctatt ttgtctctat atactacgta taggaaatgt ttacattttc
     2701 gtattgtttt cgattcactc tatgaatagt tcttactaca atttttttgt ctaaagagta
     2761 atactagaga taaacataaa aaatgtagag gtcgagttta gatgcaagtt caaggagcga
     2821 aaggtggatg ggtaggttat atagggatat agcacagaga tatatagcaa agagatactt
     2881 ttgagcaatg tttgtggaag cggtattcgc aatattttag tagctcgtta cagtccggtg
     2941 cgtttttggt tttttgaaag tgcgtcttca gagcgctttt ggttttcaaa agcgctctga
     3001 agttcctata ctttctagct agagaatagg aacttcggaa taggaacttc aaagcgtttc
     3061 cgaaaacgag cgcttccgaa aatgcaacgc gagctgcgca catacagctc actgttcacg
     3121 tcgcacctat atctgcgtgt tgcctgtata tatatataca tgagaagaac ggcatagtgc
     3181 gtgtttatgc ttaaatgcgt acttatatgc gtctatttat gtaggatgaa aggtagtcta
     3241 gtacctcctg tgatattatc ccattccatg cggggtatcg tatgcttcct tcagcactac
     3301 cctttagctg ttctatatgc tgccactccg atcttatgta tgaaattctt aaaaaaggac
     3361 acctgtaagc gttgatttct atgtatgaag tccacatttg atgtaatcat aacaaagcct
     3421 aaaaaatagg tatatcattt tataattatt tgctgtacaa gtatatcaat aaacttatat
     3481 attacttgtt ttctagataa gcttcgtaac cgacagtttc taacttttgt gctttgacaa
     3541 gaacttcttc ttcttgcttt aataaaaact gttccatttt cgttgtataa cttgaatcat
     3601 aagcgccaag cagtctgaca gccaacagcg cagcgttcgt actattatta atagcgacgg
     3661 tagctactgg aacacctcta ggcatttgca caattgaatg taaagaatct actccatcta
     3721 gacaagaacc ttttacgggc acaccgatga caggaagtgg tgtcattgca gccaccatac
     3781 ctggcaagtg agcagcccca ccagctccag cgataattgt tttaattcca cgcttgcttg
     3841 cggaaatagc atatgctgac atcctatgtg gagttctatg agcagagact attgtcactt
     3901 caaatggaac gccaaaatct tttaaaaccg cacatgcggc agacattacc ggcaagtcag
     3961 agtctgatcc catgatgatt ccaaccaatg gtttgaccat tgcttccaag tccaactttt
     4021 gagcgacaga gattttgatt ggaatatcag ttctacctgt aatgtagttc agcctttgtt
     4081 cacattccgc catactggag gcaataatat ttatgtgacc tacttttctg ttaggtctag
     4141 actcttttcc atataagtac actgaggaac ctggagtcgc caatgctctt tcgcaagttt
     4201 ctagctcttt atcttttgta tgtttgtctc caagaacatt tagcataatg gcgttcgttg
     4261 taatggtgga gaaagatgtg aaattctttg gcattggcaa atccaatatt gatctcaaat
     4321 gagcttcaaa ttgagaagtg acgcaagcat caatggtata atgtccagag ttgtgaggcc
     4381 ttggggcaat ttcgttaata agcaattccc ctgtttctaa atagaacatt tccacaccaa
     4441 atataccaca accgggaaaa gatttgattg cattttctgc caacaacttc gccttaagtt
     4501 gaacggagtc cggaactcta gcaggcgcat aacataagtc acaaatattg tccttgtgga
     4561 tagtctctac aattgggtaa gaaaacacta aaccgttaac agacctcaca atcatgactg
     4621 ctaattcttt agtaaatggt gcccattttt cggcgtacaa aggacgatcc ttcagtactt
     4681 ccaaagcttc cggaatcatt tccttattct ttacaacgaa gttacctctt ccatcgtatg
     4741 ccaaagtcct cgacttcaag acgaatggaa aacccaaatc tcttccaaca ttcaataggg
     4801 acgtctcact ggcttgttcc acaggaacac tttgggtaac tgctatacca tttttgatta
     4861 aatgctcttt ttgaatatat ttgtcttgta tcaatctgat tgtttctgga gaagggtaaa
     4921 tttttaattt gggatgtttt acttgaagat tctttagtgt aggaacatca acatgctcaa
     4981 tctcaatcgt tagcacatca catttttcag ctagtttttc gatatcaaga ggattggaaa
     5041 aggagccatt aacgtggtca ttggagttgc ttatttgttt ggcaggagaa ttttcagcat
     5101 ctagtattac cgtcttaatg ttgagcctgt ttgctgcctc aacaatcata cgtcccaatt
     5161 gtccccctcc taatatacca actgttctag aatccatact tgattgtttt gtccgatttt
     5221 cttgtttttc ttgattgtta tagtaggatg tacttagaag agagatccaa cgattttacg
     5281 caccaattta tacatgaaat gctccataat attgtccatt tagttcttaa taaaaggtca
     5341 gcaagagtca atcacttagt attacccggt tcgtagccat gcaacaagag tcatttgtca
     5401 gcatagctgt aataatcaat catgacgtaa gaaatgtatc ataattaaaa gttgttaaag
     5461 atgtcagtgt tatgttggtg ttacaaaatt ctcggcttct cactaatatt taatatctct
     5521 taaattttat ctgtctttga ttcttttaag aaaagttatg tattattcaa gaaaaagtca
     5581 attcagatcc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     5641 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     5701 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     5761 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     5821 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     5881 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     5941 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     6001 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     6061 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     6121 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     6181 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     6241 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     6301 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     6361 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     6421 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     6481 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     6541 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     6601 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     6661 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     6721 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     6781 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     6841 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     6901 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6961 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     7021 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     7081 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     7141 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     7201 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     7261 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     7321 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     7381 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     7441 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     7501 cagtgagcga ggaagcggaa ga
//
