LOCUS       40924_6192        4791 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBEM3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Park SY, Kim GJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAY-2010) Biology, Chonnam National University, 
            Yongbong-Dong 300, Buk-Gu, Gwangju, Chonnam 500-757, Korea
REFERENCE   2  (bases 1 to 4791)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4791)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (25-MAY-2010) Biology, Chonnam National University, Yongbong-Dong 
            300, Buk-Gu, Gwangju, Chonnam 500-757, Korea"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    4..2405
                     /label=BMS whole expression module
                     /note="BMS whole expression module"
     regulatory      2022..2071
                     /label=BMS promoter
                     /note="BMS promoter"
                     /regulatory_class="promoter"
     terminator      2619..2705
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2797..2824
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        2844..2935
                     /label=AmpR promoter
     CDS             2936..3793
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3967..4555
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 actgaattcc tcgccgccgc tgcgggccac ggtatcgccg ctgcgcgatc cctcggtcag
       61 cagcgtggcc agcgcttgca gcgcgcggtc gccggccgcg tggccgtagc ggtcgttgac
      121 gtgcttgaag tgatcgatgt ccaccgcgac gatcgacagc ggcgtctcgg ccgcctgcag
      181 cagcgccatg gcctcgtcca ggccgcgccg gttcagcagc ccggtcagct agtccgtggt
      241 cgaggcgcgg cgcaggtcgc ggatcttgtg ctggatcgac gacaggccca tcaacagcgc
      301 gcgcttgagc tgcgacgctt cgaaatacca ggacttcacc ttgcggatgc gctcggccga
      361 atcggggtcc tgcatgagct tggcgatgct ggccagttcg cccagcggcc gggcgatcct
      421 gcgcgacagc agacacagca gcacgatgga aagcagcagc agcggcaagg tgttgcgggc
      481 ggtcgccatc agcaggtcgt gcagcggcag cagcgtgctg gccaccggcc gctgcgcgat
      541 gatggtccag cccgtgctgg gcaccggcgc atagccggcc agtatcccgg tgccttccgc
      601 gtccgtgaag cgctgttcgc ccgtctcgcc gcgcaacgcg gccgccagtg gggccttgtc
      661 ggcggacatg tggcccaggc ggctgcggtc ggggtggtag atcagcgtgc cgtcgcgcgc
      721 cagcacgtac agcacggaat cgtcgcggtt gaagtgctgg cccagcaggc ggtccagcac
      781 gttgtcttcg tgcaggtaga gcgtgccggc gatatagccc aggtagcggt agtcgcgcga
      841 aaagatcggg tgggtgtaca ggatcagcca gcggcctgtg ctggcgcgaa agggttcgct
      901 gatgaagggg cggcgtgtcg tcagcgccgt cctggcggca tcgctggtca tctgctggcc
      961 cttcagttgc gggtacgacg gcgatgccgc caccaccagg ccttgcggcg acaccacgaa
     1021 gctggaattg aagtgtccca gttgctcttc ctggcggcgg gtttccgcca tggcgagttc
     1081 ggggtgggct tccatgtccg ccagcgtgtc ggcgctgaaa gccaggtggc ggcgcaggtc
     1141 gctcaggaaa tactcggtgg tggcggccag cttggcggca tagacgcgat tgctttccag
     1201 cgcgttgtgc agcagcaggt cgcgctggac caggtagctg gcatgcaggc ccgcggccag
     1261 gaccaggaaa atcgacagca ggctgatcca caggatgagg cgccggaggt cgatgcggaa
     1321 catggagggt gtccgtgtca ggaaggagcg cggcctggcg gcaggccgga gggcggcatg
     1381 ccgcctgtca gcgcatccca ggcggacatc gcctggcgcg ccaccgcttg cagggtcttg
     1441 cggttggcgc catcgcgcgc ctgaatggac atcccgtgca ggatcgtggt gtagaacgcc
     1501 gccaggcttt tcaggtcggc gccggttggc agttcgccgt tggccgcgcc ggcggtcagg
     1561 cgagccagga tgcagccttg catgtcgcgc cggttgtcgc ccaggaaggc gccgaccccg
     1621 tcgtggttgt cggcgccgga cggcgcgccc aatacgatca ggcagccggg gggcgcgcct
     1681 ggcgcggtga agcggtcggc ggcggccagc agcatgcctt gcacggcgtc gcgggcggta
     1741 gcggcatcgg tcagcgcacg gcgcgaggcc gcgccgtcgg tgtcgcgata aagctgcacc
     1801 gcttcgcgaa atagtccttc ttttgagccg aatgcgccgt agaggctggg cgagttgatg
     1861 cccatcgcag ccgtcagatc ggttagcgat gctccttcat atcccttcga ccagaacact
     1921 tccatggcct tgcgcaaggc ctgggctcgg tcgaagttcc gggggcgccc acgttctgcc
     1981 atgtcgcgga ttttactcgg gagaactatt ttgtgttgat cgataagaaa atcattgacg
     2041 ccgccgtgca tgtcgatgga tgatatttgt actgatcact acaaattgag gttgacccat
     2101 atggatttta agcttgcgtg cagggcaggg cgcggcggcc cgtgtgaacg ggctgtccgc
     2161 ctgcgcgaac gggccgtcga ccactttttt tggggccgat cggccagcga cttgcacaaa
     2221 taaaaaaagc tccctataat ctcgcttctt cgcagttcac acacaacgaa acgcgacggc
     2281 aacagcgcca cgctgaatgc ctgaaagatc gaagtaaatg aacatgcagg gctgttagct
     2341 cagttggtag agcagcggac tcttaatccg taggtcgagt gttcgagcca ctcacagccc
     2401 accagaattc gctagaggct gttttggcgg atgagagaag attttcagcc tgatacagat
     2461 taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca gtagcgcggt
     2521 ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt
     2581 ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt
     2641 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
     2701 caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag
     2761 gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg acggatggcc
     2821 tttttgcgtt tctacaaact ctttttgttt atttttctaa atacattcaa atatgtatcc
     2881 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     2941 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     3001 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     3061 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     3121 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt
     3181 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     3241 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     3301 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     3361 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     3421 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctac
     3481 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     3541 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     3601 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     3661 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     3721 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     3781 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     3841 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     3901 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     3961 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     4021 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     4081 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     4141 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     4201 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     4261 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     4321 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     4381 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     4441 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     4501 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     4561 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     4621 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     4681 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     4741 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca g
//
