LOCUS       pBE2R        7011 bp DNA     circular SYN 30-DEC-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7011)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7011)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        799..903
                     /label=AmpR promoter
     CDS             904..1761
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LMKHW"
     rep_origin      1935..2523
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4860..5861)
                     /codon_start=1
                     /label=repB
                     /note="RepB replication protein"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
     CDS             6980..6997
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
ORIGIN
        1 tagtgtcacc taaatcgtat gtgtatgata cataaggtta tgtattaatt gtagccgcgt
       61 tctaacgaca atatgtacaa gcctaattgt gtagcatctg gcttactgaa gcagacccta
      121 tcatctctct cgtaaactgc cgtcagagtc ggtttggttg gacgaacctt ctgagtttct
      181 ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa tcagcagggt catcgctagc
      241 cagatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct
      301 ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg
      361 agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc
      421 atctccttgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct
      481 gcttcctaat gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc
      541 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
      601 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
      661 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
      721 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
      781 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      841 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
      901 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
      961 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     1021 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     1081 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     1141 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     1201 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     1261 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     1321 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     1381 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     1441 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     1501 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     1561 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     1621 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     1681 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     1741 gcctcactga tgaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     1801 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     1861 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     1921 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     1981 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2041 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     2101 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     2161 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     2221 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     2281 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     2341 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2401 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2461 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2521 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2581 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     2641 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     2701 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     2761 tgatttcact ttttgcattc tacaaactgc ataactcata tgtaaatcgc tcctttttag
     2821 gtggcacaaa tgtgaggcat tttcgctctt tccggcaacc acttccaagt aaagtataac
     2881 acactatact ttatattcat aaagtgtgtg ctctgcgagg ctgtcggcag tgccgaccaa
     2941 aaccataaaa cctttaagac ctttcttttt tttacgagaa aaaagaaaca aaaaaacctg
     3001 ccctctgcca cctcagcaaa ggggggtttt gctctcgtgc tcgtttaaaa atcagcaagg
     3061 gacaggtagt attttttgag aagatcactc aaaaaatctc cacctttaaa cccttgccaa
     3121 tttttatttt gtccgttttg tctagcttac cgaaagccag actcagcaag aataaaattt
     3181 ttattgtctt tcggttttct agtgtaacgg acaaaaccac tcaaaataaa aaagatacaa
     3241 gagaggtctc tcgtatcttt tattcagcaa tcgcgcccga ttgctgaaca gattaataat
     3301 agattttagc tttttatttg ttgaaaaaag ctaatcaaat tgttgtcggg atcaattact
     3361 gcaaagtctc gttcatccca ccactgatct tttaatgatg tattggggtg caaaatgccc
     3421 aaaggcttaa tatgttgata taattcatca attccctcta cttcaatgcg gcaactagca
     3481 gtaccagcaa taaacgactc cgcacctgta caaaccggtg aatcattact acgagagcgc
     3541 cagccttcat cacttgcctc ccatagatga atccgaacct cattacacat tagaactgcg
     3601 aatccatctt catggtgaac caaagtgaaa cctagtttat cgcaataaaa acctatactc
     3661 tttttaatat ccccgactgg caatgccggg atagactgta acattctcac gcataaaatc
     3721 ccctttcatt ttctaatgta aatctattac cttattatta attcaattcg ctcataatta
     3781 atcctttttc ttattacgca aaatggcccg atttaagcac accctttatt ccgttaatgc
     3841 gccatgacag ccatgataat tactaatact aggagaagtt aataaatacg taaccaacat
     3901 gattaacaat tattagaggt catcgttcaa aatggtatgc gttttgacac atccactata
     3961 tatccgtgtc gttctgtcca ctcctgaatc ccattccaga aattctctag cgattccaga
     4021 agtttctcag agtcggaaag ttgaccagac attacgaact ggcacagatg gtcataacct
     4081 gaaggaagat ctgattgctt aactgcttca gttaagaccg aagcgctcgt cgtataacag
     4141 atgcgatgat gcagaccaat caacatggca cctgccattg ctacctgtac agtcaaggat
     4201 ggtagaaatg ttgtcggtcc ttgcacacga atattacgcc atttgcctgc atattcaaac
     4261 agctcttcta cgataagggc acaaatcgca tcgtggaacg tttgggcttc taccgattta
     4321 gcagtttgat acactttctc taagtatcca cctgaatcat aaatcggcaa aatagagaaa
     4381 aattgaccat gtgtaagcgg ccaatctgat tccacctgag atgcataatc tagtagaatc
     4441 tcttcgctat caaaattcac ttccaccttc cactcaccgg ttgtccattc atggctgaac
     4501 tctgcttcct ctgttgacat gacacacatc atctcaatat ccgaataggg cccatcagtc
     4561 tgacgaccaa gagagccata aacaccaata gccttaacat catccccata tttatccaat
     4621 attcgttcct taatttcatg aacaatcttc attctttctt ctctagtcat tattattggt
     4681 ccattcacta ttctcattcc cttttcagat aattttagat ttgcttttct aaataagaat
     4741 atttggagag caccgttctt attcagctat taataactcg tcttcctaag catccttcaa
     4801 tccttttaat aacaattata gcatctaatc ttcaacaaac tggcccgttt gttgaactac
     4861 tctttaataa aataattttt ccgttcccaa ttccacattg caataataga aaatccatct
     4921 tcatcggctt tttcgtcatc atctgtatga atcaaatcgc cttcttctgt gtcatcaagg
     4981 tttaattttt tatgtatttc ttttaacaaa ccaccatagg agattaacct tttacggtgt
     5041 aaaccttcct ccaaatcaga caaacgtttc aaattctttt cttcatcatc ggtcataaaa
     5101 tccgtatcct ttacaggata ttttgcagtt tcgtcaattg ccgattgtat atccgattta
     5161 tatttatttt tcggtcgaat catttgaact tttacatttg gatcatagtc taatttcatt
     5221 gcctttttcc aaaattgaat ccattgtttt tgattcacgt agttttctgt attcttaaaa
     5281 taagttggtt ccacacatac caatacatgc atgtgctgat tataagaatt atctttatta
     5341 tttattgtca cttccgttgc acgcataaaa ccaacaagat ttttattaat ttttttatat
     5401 tgcatcattc ggcgaaatcc ttgagccata tctgacaaac tcttatttaa ttcttcgcca
     5461 tcataaacat ttttaactgt taatgtgaga aacaaccaac gaactgttgg cttttgttta
     5521 ataacttcag caacaacctt ttgtgactga atgccatgtt tcattgctct cctccagttg
     5581 cacattggac aaagcctgga tttacaaaac cacactcgat acaactttct ttcgcctgtt
     5641 tcacgatttt gtttatactc taatatttca gcacaatctt ttactctttc agccttttta
     5701 aattcaagaa tatgcagaag ttcaaagtaa tcaacattag cgattttctt ttctctccat
     5761 ggtctcactt ttccactttt tgtcttgtcc actaaaaccc ttgatttttc atctgaataa
     5821 atgctactat taggacacat aatattaaaa gaaaccccca tctatttagt tatttgttta
     5881 gtcacttata actttaacag atggggtttt tctgtgcaac caattttaag ggttttcaat
     5941 actttaaaac acatacatac caacacttca acgcaccttt cagcaactaa aataaaaatg
     6001 acgttatttc tatatgtatc aagataagaa agaacaagtt caaaaccatc aaaaaaagac
     6061 accttttcag gtgctttttt tattttataa actcattccc tgatctcgac ttcgttcttt
     6121 ttttacctct cggttatgag ttagttcaaa ttcgttcttt ttaggttcta aatcgtgttt
     6181 ttcttggaat tgtgctgttt tatcctttac cttgtctaca aaccccttaa aaacgttttt
     6241 aaaggctttt aagccgtctg tacgttcctt aaggaattcg agctcagcat tattgagtgg
     6301 atgattatat tccttttgat aggtggtatg ttttcgcttg aacttttaaa tacagccatt
     6361 gaacatacgg ttgatttaat aactgacaaa catcaccctc ttgctaaagc ggccaaggac
     6421 gctgccgccg gggctgtttg cgtttttgcc gtgatttcgt gtattattgg tttacttatt
     6481 tttttgccaa agctgtaatg gctgaaaatt cttacattta ttttacattt ttagaaatgg
     6541 gcgtgaaaaa aagcgcgcga ttatgtaaaa tataaagtga tagcggtacc attataggta
     6601 agagaggaat gtacacatga agaaaccgtt ggggaaaatt gtcgcaagca ccgcactact
     6661 catttctgtt gcttttagtt catcgatcgc atcggctgct gaagaagcaa aagaaaaata
     6721 tttaattggc tttaatgagc aggaagctgt cagtgagttt gtagaacaag tagaggcaaa
     6781 tgacgaggtc gccattctct ctgaggaaga ggaagtcgaa attgaattgc ttcatgaatt
     6841 tgaaacgatt cctgttttat ccgttgagtt aagcccagaa gatgtggacg cgcttgaact
     6901 cgatccagcg atttcttata ttgaagagga tgcagaagta acgacaatgg gatcctctag
     6961 agtcgacctg cagctcgagc accaccacca ccaccacaag cttgtattct a
//
