LOCUS       V015036                 8215 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V015036
VERSION     V015036
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8215)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      891..1479
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3531..4178)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(4504..5505)
                     /label="repB"
                     /note="RepB replication protein"
     CDS             6273..6947
                     /label="mRFP1"
                     /note="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
     terminator      6995..7066
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      7082..7109
                     /label="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     promoter        complement(7167..7185)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        7970..8074
                     /label="AmpR promoter"
     CDS             join(8075..8215,1..717)
                     /label="AmpR"
                     /note="beta-lactamase"
ORIGIN
        1 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
       61 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
      121 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
      181 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
      241 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
      301 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
      361 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
      421 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
      481 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
      541 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
      601 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
      661 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
      721 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
      781 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
      841 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
      901 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
      961 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     1021 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     1081 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     1141 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     1201 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     1261 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     1321 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     1381 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     1441 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     1501 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     1561 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     1621 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     1681 cctctccccg cgcgttggcc gattcattaa tgcagctgat ttcacttttt gcattctaca
     1741 aactgcataa ctcatatgta aatcgctcct ttttaggtgg cacaaatgtg aggcattttc
     1801 gctctttccg gcaaccactt ccaagtaaag tataacacac tatactttat attcataaag
     1861 tgtgtgctct gcgaggctgt cggcagtgcc gaccaaaacc ataaaacctt taagaccttt
     1921 ctttttttta cgagaaaaaa gaaacaaaaa aacctgccct ctgccacctc agcaaagggg
     1981 ggttttgctc tcgtgctcgt ttaaaaatca gcaagggaca ggtagtattt tttgagaaga
     2041 tcactcaaaa aatctccacc tttaaaccct tgccaatttt tattttgtcc gttttgtcta
     2101 gcttaccgaa agccagactc agcaagaata aaatttttat tgtctttcgg ttttctagtg
     2161 taacggacaa aaccactcaa aataaaaaag atacaagaga ggtctctcgt atcttttatt
     2221 cagcaatcgc gcccgattgc tgaacagatt aataatagat tttagctttt tatttgttga
     2281 aaaaagctaa tcaaattgtt gtcgggatca attactgcaa agtctcgttc atcccaccac
     2341 tgatctttta atgatgtatt ggggtgcaaa atgcccaaag gcttaatatg ttgatataat
     2401 tcatcaattc cctctacttc aatgcggcaa ctagcagtac cagcaataaa cgactccgca
     2461 cctgtacaaa ccggtgaatc attactacga gagcgccagc ttcatcactt gcctcccata
     2521 gatgaatccg aacctcatta cacattagaa ctgcgaatcc atcttcatgg tgaaccaaag
     2581 tgaaacctag tttatcgcaa taaaaaccta tactcttttt aatatccccg actggcaatg
     2641 ccgggataga ctgtaacatt ctcacgcata aaatcccctt tcattttcta atgtaaatct
     2701 attaccttat tattaattca attcgctcat aattaatcct ttttcttatt acgcaaaatg
     2761 gcccgattta agcacaccct ttattccgtt aatgcgccat gacagccatg ataattacta
     2821 atactaggag aagttaataa atacgtaacc aacatgatta acaattatta gaggtcatcg
     2881 ttcaaaatgg tatgcgtttt gacacatcca ctatatatcc gtgtcgttct gtccactcct
     2941 gaatcccatt ccagaaattc tctagcgatt ccagaagttt ctcagagtcg gaaagttgac
     3001 cagacattac gaactggcac agatggtcat aacctgaagg aagatctgat tgcttaactg
     3061 cttcagttaa gaccgaagcg ctcgtcgtat aacagatgcg atgatgcaga ccaatcaaca
     3121 tggcacctgc cattgctacc tgtacagtca aggatggtag aaatgttgtc ggtccttgca
     3181 cacgaatatt acgccatttg cctgcatatt caaacagctc ttctacgata agggcacaaa
     3241 tcgcatcgtg gaacgtttgg gcttctaccg atttagcagt ttgatacact ttctctaagt
     3301 atccacctga atcataaatc ggcaaaatag agaaaaattg accatgtgta agcggccaat
     3361 ctgattccac ctgagatgca taatctagta gaatctcttc gctatcaaaa ttcacttcca
     3421 ccttccactc accggttgtc cattcatggc tgaactctgc ttcctctgtt gacatgacac
     3481 acatcatctc aatatccgaa tagggccaca gtcggcatta tctcatatta taaaagccag
     3541 tcattaggcc tatctgacaa ttcctgaata gagttcataa acaatcctgc atgataacca
     3601 tcacaaacag aatgatgtac ctgtaaagat agcggtaaat atattgaatt acctttatta
     3661 atgaattttc ctgctgtaat aatgggtaga aggtaattac tattattatt gatatttaag
     3721 ttaaacccag taaatgaagt ccatggaata atagaaagag aaaaagcatt ttcaggtata
     3781 ggtgttttgg gaaacaattt ccccgaacca ttatatttct ctacatcaga aaggtataaa
     3841 tcataaaact ctttgaagtc attctttaca ggagtccaaa taccagagaa tgttttagat
     3901 acaccatcaa aaattgtata aagtggctct aacttatccc aataacctaa ctctccgtcg
     3961 ctattgtaac cagttctaaa agctgtattt gagtttatca cccttgtcac taagaaaata
     4021 aatgcagggt aaaatttata tccttcttgt tttatgtttc ggtataaaac actaatatca
     4081 atttctgtgg ttatactaaa agtcgtttgt tggttcaaat aatgattaaa tatctctttt
     4141 ctcttccaat tgtctaaatc aattttatta aagttcattt gatatgcctc ctggcccatc
     4201 agtctgacga ccaagagagc cataaacacc aatagcctta acatcatccc catatttatc
     4261 caatattcgt tccttaattt catgaacaat cttcattctt tcttctctag tcattattat
     4321 tggtccattc actattctca ttcccttttc agataatttt agatttgctt ttctaaataa
     4381 gaatatttgg agagcaccgt tcttattcag ctattaataa ctcgtcttcc taagcatcct
     4441 tcaatccttt taataacaat tatagcatct aatcttcaac aaactggccc gtttgttgaa
     4501 ctactcttta ataaaataat ttttccgttc ccaattccac attgcaataa tagaaaatcc
     4561 atcttcatcg gctttttcgt catcatctgt atgaatcaaa tcgccttctt ctgtgtcatc
     4621 aaggtttaat tttttatgta tttcttttaa caaaccacca taggagatta accttttacg
     4681 gtgtaaacct tcctccaaat cagacaaacg tttcaaattc ttttcttcat catcggtcat
     4741 aaaatccgta tcctttacag gatattttgc agtttcgtca attgccgatt gtatatccga
     4801 tttatattta tttttcggtc gaatcatttg aacttttaca tttggatcat agtctaattt
     4861 cattgccttt ttccaaaatt gaatccattg tttttgattc acgtagtttt ctgtattctt
     4921 aaaataagtt ggttccacac ataccaatac atgcatgtgc tgattataag aattatcttt
     4981 attatttatt gtcacttccg ttgcacgcat aaaaccaaca agatttttat taattttttt
     5041 atattgcatc attcggcgaa atccttgagc catatctgac aaactcttat ttaattcttc
     5101 gccatcataa acatttttaa ctgttaatgt gagaaacaac caacgaactg ttggcttttg
     5161 tttaataact tcagcaacaa ccttttgtga ctgaatgcca tgtttcattg ctctcctcca
     5221 gttgcacatt ggacaaagcc tggatttaca aaaccacact cgatacaact ttctttcgcc
     5281 tgtttcacga ttttgtttat actctaatat ttcagcacaa tcttttactc tttcagcctt
     5341 tttaaattca agaatatgca gaagttcaaa gtaatcaaca ttagcgattt tcttttctct
     5401 ccatggtctc acttttccac tttttgtctt gtccactaaa acccttgatt tttcatctga
     5461 ataaatgcta ctattaggac acataatatt aaaagaaacc cccatctatt tagttatttg
     5521 tttagtcact tataacttta acagatgggg tttttctgtg caaccaattt taagggtttt
     5581 caatacttta aaacacatac ataccaacac ttcaacgcac ctttcagcaa ctaaaataaa
     5641 aatgacgtta tttctatatg tatcaagata agaaagaaca agttcaaaac catcaaaaaa
     5701 agacaccttt tcaggtgctt tttttatttt ataaactcat tccctgatct cgacttcgtt
     5761 ctttttttac ctctcggtta tgagttagtt caaattcgtt ctttttaggt tctaaatcgt
     5821 gtttttcttg gaattgtgct gttttatcct ttaccttgtc tacaaacccc ttaaaaacgt
     5881 ttttaaaggc ttttaagccg tctgtacgtt ccttaaggaa ttcgagctca gcattattga
     5941 gtggatgatt atattccttt tgataggtgg tatgttttcg cttgaacttt taaatacagc
     6001 cattgaacat acggttgatt taataactga caaacatcac cctcttgcta aagcggccaa
     6061 ggacgctgcc gccggggctg tttgcgtttt tgccgtgatt tcgtgtatta ttggtttact
     6121 tatttttttg ccaaagctgt aatggctgaa aattcttaca tttattttac atttttagaa
     6181 atgggcgtga aaaaaagcgc gcgattatgt aaaatataaa gtgatagcgg taccattata
     6241 cacacatact agagaaagag gagaaatact agatggcttc ctccgaagac gttatcaaag
     6301 agttcatgcg tttcaaagtt cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg
     6361 aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg aaagttacca
     6421 aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc gcagttccag tacggttcca
     6481 aagcttacgt taaacacccg gctgacatcc cggactacct gaaactgtcc ttcccggaag
     6541 gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc gttacccagg
     6601 actcctccct gcaagacggt gagttcatct acaaagttaa actgcgtggt accaacttcc
     6661 cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta
     6721 tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg
     6781 gtggtcacta cgacgctgaa gttaaaacca cctacatggc taaaaaaccg gttcagctgc
     6841 cgggtgctta caaaaccgac atcaaactgg acatcacctc ccacaacgaa gactacacca
     6901 tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa taacgctgat
     6961 agtgctagtg tagatcgcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
     7021 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     7081 tggctcacct tcgggtgggc ctttctgcgt ttatataggc catctgggcc gggggcgtct
     7141 ctgagctaga aatagcaagc ttgtattcta tagtgtcacc taaatcgtat gtgtatgata
     7201 cataaggtta tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt
     7261 gtagcatctg gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc
     7321 ggtttggttg gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc
     7381 agcgaaccaa tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc
     7441 ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg
     7501 gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca
     7561 ggccccgtgg ccgggggact gttgggcgcc atctccttgc accattcctt gcggcggcgg
     7621 tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag
     7681 agcgtcgata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     7741 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     7801 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     7861 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     7921 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     7981 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     8041 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     8101 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     8161 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactg
//
