LOCUS       62056_3775        7332 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7332)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1570..2571)
                     /codon_start=1
                     /label=repB
                     /note="RepB replication protein"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
     CDS             3317..4030
                     /codon_start=1
                     /label=mEYFP
                     /note="enhanced YFP with monomerizing A206K mutation
                     (Zacharias et al., 2002)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     promoter        complement(4039..4057)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        4842..4946
                     /label=AmpR promoter
     CDS             4947..5804
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5978..6566
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agattaataa tagattttag ctttttattt gttgaaaaaa gctaatcaaa ttgttgtcgg
       61 gatcaattac tgcaaagtct cgttcatccc accactgatc ttttaatgat gtattggggt
      121 gcaaaatgcc caaaggctta atatgttgat ataattcatc aattccctct acttcaatgc
      181 ggcaactagc agtaccagca ataaacgact ccgcacctgt acaaaccggt gaatcattac
      241 tacgagagcg ccagcttcat cacttgcctc ccatagatga atccgaacct cattacacat
      301 tagaactgcg aatccatctt catggtgaac caaagtgaaa cctagtttat cgcaataaaa
      361 acctatactc tttttaatat ccccgactgg caatgccggg atagactgta acattctcac
      421 gcataaaatc ccctttcatt ttctaatgta aatctattac cttattatta attcaattcg
      481 ctcataatta atcctttttc ttattacgca aaatggcccg atttaagcac accctttatt
      541 ccgttaatgc gccatgacag ccatgataat tactaatact aggagaagtt aataaatacg
      601 taaccaacat gattaacaat tattagaggt catcgttcaa aatggtatgc gttttgacac
      661 atccactata tatccgtgtc gttctgtcca ctcctgaatc ccattccaga aattctctag
      721 cgattccaga agtttctcag agtcggaaag ttgaccagac attacgaact ggcacagatg
      781 gtcataacct gaaggaagat ctgattgctt aactgcttca gttaagaccg aagcgctcgt
      841 cgtataacag atgcgatgat gcagaccaat caacatggca cctgccattg ctacctgtac
      901 agtcaaggat ggtagaaatg ttgtcggtcc ttgcacacga atattacgcc atttgcctgc
      961 atattcaaac agctcttcta cgataagggc acaaatcgca tcgtggaacg tttgggcttc
     1021 taccgattta gcagtttgat acactttctc taagtatcca cctgaatcat aaatcggcaa
     1081 aatagagaaa aattgaccat gtgtaagcgg ccaatctgat tccacctgag atgcataatc
     1141 tagtagaatc tcttcgctat caaaattcac ttccaccttc cactcaccgg ttgtccattc
     1201 atggctgaac tctgcttcct ctgttgacat gacacacatc atctcaatat ccgaataggg
     1261 cccatcagtc tgacgaccaa gagagccata aacaccaata gccttaacat catccccata
     1321 tttatccaat attcgttcct taatttcatg aacaatcttc attctttctt ctctagtcat
     1381 tattattggt ccattcacta ttctcattcc cttttcagat aattttagat ttgcttttct
     1441 aaataagaat atttggagag caccgttctt attcagctat taataactcg tcttcctaag
     1501 catccttcaa tccttttaat aacaattata gcatctaatc ttcaacaaac tggcccgttt
     1561 gttgaactac tctttaataa aataattttt ccgttcccaa ttccacattg caataataga
     1621 aaatccatct tcatcggctt tttcgtcatc atctgtatga atcaaatcgc cttcttctgt
     1681 gtcatcaagg tttaattttt tatgtatttc ttttaacaaa ccaccatagg agattaacct
     1741 tttacggtgt aaaccttcct ccaaatcaga caaacgtttc aaattctttt cttcatcatc
     1801 ggtcataaaa tccgtatcct ttacaggata ttttgcagtt tcgtcaattg ccgattgtat
     1861 atccgattta tatttatttt tcggtcgaat catttgaact tttacatttg gatcatagtc
     1921 taatttcatt gcctttttcc aaaattgaat ccattgtttt tgattcacgt agttttctgt
     1981 attcttaaaa taagttggtt ccacacatac caatacatgc atgtgctgat tataagaatt
     2041 atctttatta tttattgtca cttccgttgc acgcataaaa ccaacaagat ttttattaat
     2101 ttttttatat tgcatcattc ggcgaaatcc ttgagccata tctgacaaac tcttatttaa
     2161 ttcttcgcca tcataaacat ttttaactgt taatgtgaga aacaaccaac gaactgttgg
     2221 cttttgttta ataacttcag caacaacctt ttgtgactga atgccatgtt tcattgctct
     2281 cctccagttg cacattggac aaagcctgga tttacaaaac cacactcgat acaactttct
     2341 ttcgcctgtt tcacgatttt gtttatactc taatatttca gcacaatctt ttactctttc
     2401 agccttttta aattcaagaa tatgcagaag ttcaaagtaa tcaacattag cgattttctt
     2461 ttctctccat ggtctcactt ttccactttt tgtcttgtcc actaaaaccc ttgatttttc
     2521 atctgaataa atgctactat taggacacat aatattaaaa gaaaccccca tctatttagt
     2581 tatttgttta gtcacttata actttaacag atggggtttt tctgtgcaac caattttaag
     2641 ggttttcaat actttaaaac acatacatac caacacttca acgcaccttt cagcaactaa
     2701 aataaaaatg acgttatttc tatatgtatc aagataagaa agaacaagtt caaaaccatc
     2761 aaaaaaagac accttttcag gtgctttttt tattttataa actcattccc tgatctcgac
     2821 ttcgttcttt ttttacctct cggttatgag ttagttcaaa ttcgttcttt ttaggttcta
     2881 aatcgtgttt ttcttggaat tgtgctgttt tatcctttac cttgtctaca aaccccttaa
     2941 aaacgttttt aaaggctttt aagccgtctg tacgttcctt aaggaattcg agctcagcat
     3001 tattgagtgg atgattatat tccttttgat aggtggtatg ttttcgcttg aacttttaaa
     3061 tacagccatt gaacatacgg ttgatttaat aactgacaaa catcaccctc ttgctaaagc
     3121 ggccaaggac gctgccgccg gggctgtttg cgtttttgcc gtgatttcgt gtattattgg
     3181 tttacttatt tttttgccaa agctgtaatg gctgaaaatt cttacattta ttttacattt
     3241 ttagaaatgg gcgtgaaaaa aagcgcgcga ttatgtaaaa tataaagtga tagcggtaca
     3301 agagaggaat gtacacatga gcaaaggcga agaactgttt accggtgttg ttccgattct
     3361 ggttgaactg gatggtgatg ttaatggcca caaattttca gttagcggtg aaggcgaagg
     3421 tgatgcaacc tatggtaaac tgaccctgaa atttatctgt accaccggca aactgccggt
     3481 tccgtggccg acactggtta ccacctttgg ttatggtctg atgtgttttg cacgttatcc
     3541 ggatcatatg aaacagcacg attttttcaa aagcgcaatg ccggaaggtt atgttcaaga
     3601 acgtaccatc ttcttcaaag atgacggcaa ctataaaacc cgtgccgaag ttaaatttga
     3661 aggtgatacc ctggtgaatc gcattgaact gaaaggcatc gattttaaag aggatggtaa
     3721 tatcctgggc cacaaactgg aatataatta taatagccac aacgtgtaca tcatggccga
     3781 caaacagaaa aatggcatca aagtgaactt caagatccgc cataatattg aagatggttc
     3841 agttcagctg gccgatcatt atcagcagaa taccccgatt ggtgatggtc cggttctgct
     3901 gccggataat cattatctga gctatcagag caaactgagc aaagatccga atgaaaaacg
     3961 tgatcacatg gtgctgctgg aatttgttac cgcagcaggt attaccttag gtatggatga
     4021 actgtacaaa taatgtattc tatagtgtca cctaaatcgt atgtgtatga tacataaggt
     4081 tatgtattaa ttgtagccgc gttctaacga caatatgtac aagcctaatt gtgtagcatc
     4141 tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag tcggtttggt
     4201 tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc
     4261 aatcagcagg gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg ccggcatcac
     4321 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg
     4381 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt
     4441 ggccggggga ctgttgggcg ccatctcctt gcaccattcc ttgcggcggc ggtgctcaac
     4501 ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga
     4561 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     4621 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     4681 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     4741 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     4801 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     4861 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     4921 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     4981 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     5041 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     5101 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     5161 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     5221 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     5281 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     5341 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     5401 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     5461 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     5521 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     5581 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     5641 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     5701 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     5761 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     5821 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     5881 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     5941 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6001 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6061 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6121 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6181 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6241 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     6301 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     6361 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     6421 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     6481 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     6541 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     6601 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     6661 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     6721 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     6781 gttggccgat tcattaatgc agctgatttc actttttgca ttctacaaac tgcataactc
     6841 atatgtaaat cgctcctttt taggtggcac aaatgtgagg cattttcgct ctttccggca
     6901 accacttcca agtaaagtat aacacactat actttatatt cataaagtgt gtgctctgcg
     6961 aggctgtcgg cagtgccgac caaaaccata aaacctttaa gacctttctt ttttttacga
     7021 gaaaaaagaa acaaaaaaac ctgccctctg ccacctcagc aaaggggggt tttgctctcg
     7081 tgctcgttta aaaatcagca agggacaggt agtatttttt gagaagatca ctcaaaaaat
     7141 ctccaccttt aaacccttgc caatttttat tttgtccgtt ttgtctagct taccgaaagc
     7201 cagactcagc aagaataaaa tttttattgt ctttcggttt tctagtgtaa cggacaaaac
     7261 cactcaaaat aaaaaagata caagagaggt ctctcgtatc ttttattcag caatcgcgcc
     7321 cgattgctga ac
//
