LOCUS       40924_6167        5497 bp DNA     circular SYN 17-DEC-2018
DEFINITION  EGFP expression vector pBE, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Colas J-F., Schoenwolf GC.
  TITLE     Assessing the contribution of gene products to the form-shaping 
            events of neurulation: a transgenic approach in chick
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5497)
  AUTHORS   Colas J-F.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2003) Neurobiology and Anatomy, University of 
            Utah, 20 North 1900 East MREB401, Salt Lake City, UT 84132-3401, USA
REFERENCE   3  (bases 1 to 5497)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5497)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-FEB-2003) Neurobiology and Anatomy, University of Utah, 20 North
            1900 East MREB401, Salt Lake City, UT 84132-3401, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        57..334
                     /label=chicken beta-actin promoter
     intron          335..1344
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     exon            1345..1398
                     /note="Oryctolagus cuniculus beta1-globin gene, GenBank 
                     Accession Number K03256; exon 3"
     CDS             1443..2159
                     /label=EGFP
                     /note="enhanced GFP"
     polyA_signal    2285..2406
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2413..2868)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2895..2999
                     /label=AmpR promoter
     promoter        3001..3358
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3393..4184
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4419..4466
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4795..5383
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattac tagcgctacc ggactcagat ctcgagctca agcttcgaat tcatggtcga
       61 ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt
      121 gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg ggggggggcg
      181 cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc
      241 ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg
      301 gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgttgc cttcgccccg
      361 tgccccgctc cgccgcgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc
      421 acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg
      481 acggctcgtt tcttttctgt ggctgcgtga aagccttaag aggctccggg agggcccttt
      541 gtgcgggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt
      601 gcggcccgcg ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct
      661 ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg
      721 cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg
      781 cgcgtcggtc gggctgcaac cccccctgca cccccctccc cgagttgctg agcacggccc
      841 ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg
      901 gtggcggcag gtgggggtgc cgggcggggc ggggccgcct cgggccgggg agggctcggg
      961 ggaggggcgc ggcggccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat
     1021 tgccttttat ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctggcgg
     1081 agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcgggcga agcggtgcgg
     1141 cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct
     1201 tctccatctc cagcctcggg gctgccgcag ggggacggct gccttcgggg gggacggggc
     1261 agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt
     1321 catgccttct tctttttcct acagctcctg ggcaacgtgc tggttgttgt gctgtctcat
     1381 cattttggca aagaattctg cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca
     1441 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     1501 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     1561 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     1621 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     1681 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     1741 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     1801 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     1861 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     1921 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     1981 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     2041 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     2101 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     2161 aaagcggccg cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc
     2221 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     2281 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     2341 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     2401 atcttaaggc gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     2461 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     2521 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     2581 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     2641 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     2701 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     2761 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     2821 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt
     2881 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     2941 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc
     3001 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     3061 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     3121 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     3181 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     3241 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     3301 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga
     3361 tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     3421 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     3481 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     3541 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc
     3601 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     3661 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     3721 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     3781 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     3841 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     3901 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg
     3961 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     4021 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     4081 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     4141 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     4201 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     4261 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     4321 ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac
     4381 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa
     4441 aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc
     4501 tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc
     4561 accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg
     4621 ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta
     4681 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     4741 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     4801 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     4861 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     4921 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     4981 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     5041 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     5101 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     5161 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     5221 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     5281 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     5341 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     5401 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     5461 ccctgattct gtggataacc gtattaccgc catgcat
//
