LOCUS       V037588                 5873 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037588
VERSION     V037588
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        281..309
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
                     /label="tet promoter"
     CDS             357..1547
                     /codon_start=1
                     /gene="tet"
                     /note="confers resistance to tetracycline"
                     /product="tetracycline efflux protein"
                     /transl_table=1
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
                     /label="TcR"
     protein_bind    1741..1762
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        1777..1807
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    1815..1831
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     primer_bind     1839..1855
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     promoter        1876..1894
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /label="T3 promoter"
     CDS             1913..2623
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK*"
                     /label="mCherry"
     promoter        complement(2668..2686)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     primer_bind     complement(2696..2712)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     CDS             complement(3419..4081)
                     /codon_start=1
                     /experiment=""
                     /gene=""
                     /note=""
                     /product="replication protein for the broad-host-range
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /protein_id=""
                     /transl_table=1
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR*"
                     /label="pBBR1 Rep"
     rep_origin      4082..4851
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1
                     Rep protein for replication"
                     /label="pBBR1 oriV"
ORIGIN
        1 accttcggga gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc
       61 aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg
      121 aagtccagcg ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg
      181 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
      241 gcgtatcacg aggccctttc gtcttcaaga attctcatgt ttgacagctt atcatcgata
      301 agctttaatg cggtagttta tcacagttaa attgctaacg cagtcaggca ccgtgtatga
      361 aatctaacaa tgcgctcatc gtcatcctcg gcaccgtcac cctggatgct gtaggcatag
      421 gcttggttat gccggtactg ccgggcctct tgcgggatat cgtccattcc gacagcatcg
      481 ccagtcacta tggcgtgctg ctagcgctat atgcgttgat gcaatttcta tgcgcacccg
      541 ttctcggagc actgtccgac cgctttggcc gccgcccagt cctgctcgct tcgctacttg
      601 gagccactat cgactacgcg atcatggcga ccacacccgt cctgtggatc ctctacgccg
      661 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
      721 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg
      781 tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac
      841 cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc
      901 aggagtcgca taagggagag cgtcgaccga tgcccttgag agccttcaac ccagtcagct
      961 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca
     1021 tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc
     1081 gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg
     1141 ctcaagcctt cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg
     1201 ccggcatggc ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga
     1261 tggccttccc cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg
     1321 ccatgctgtc caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg
     1381 ctcttaccag cctaacttcg atcactggac cgctgatcgt cacggcgatt tatgccgcct
     1441 cggcgagcac atggaacggg ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc
     1501 tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc gacctgaatg gaagccggcg
     1561 gcacctcgct aacggattca ccactccaag aattggagcc aatcaattct tgcggagaac
     1621 tgtgaatgcg caaatgcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     1681 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     1741 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     1801 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     1861 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctgggta ccatggtgag
     1921 caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat
     1981 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     2041 cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg
     2101 ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga
     2161 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     2221 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt
     2281 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa
     2341 gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa
     2401 gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa
     2461 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa
     2521 gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga
     2581 gggccgccac tccaccggcg gcatggacga gctgtacaag tagactagtt ctagagcggc
     2641 cgccaccgcg gtggagctcc aattcgccct atagtgagtc gtattacgcg cgctcactgg
     2701 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     2761 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     2821 cccaacagtt gcgcagcctg aatggcgaat ggaaattgta agcgttaata ttttgttaaa
     2881 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg actgcgatga
     2941 gtggcagggc ggggcgtaat ttttttaagg cagttattgg tgcccttaaa cgcctggtgc
     3001 tacgcctgaa taagtgataa taagcggatg aatggcagaa attcgaaagc aaattcgacc
     3061 cggtcgtcgg ttcagggcag ggtcgttaaa tagccgctta tgtctattgc tggtttaccg
     3121 gtttattgac taccggaagc agtgtgaccg tgtgcttctc aaatgcctga ggccagtttg
     3181 ctcaggctct ccccgtggag gtaataattg acgatatgat catttattct gcctcccaga
     3241 gcctgataaa aacggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt
     3301 ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgag
     3361 gcggctacag ccgatagtct ggaacagcgc acttacgggt tgctgcgcaa cccaagtgct
     3421 accggcgcgg cagcgtgacc cgtgtcggcg gctccaacgg ctcgccatcg tccagaaaac
     3481 acggctcatc gggcatcggc aggcgctgct gcccgcgccg ttcccattcc tccgtttcgg
     3541 tcaaggctgg caggtctggt tccatgcccg gaatgccggg ctggctgggc ggctcctcgc
     3601 cggggccggt cggtagttgc tgctcgcccg gatacagggt cgggatgcgg cgcaggtcgc
     3661 catgccccaa cagcgattcg tcctggtcgt cgtgatcaac caccacggcg gcactgaaca
     3721 ccgacaggcg caactggtcg cggggctggc cccacgccac gcggtcattg accacgtagg
     3781 ccgacacggt gccggggccg ttgagcttca cgacggagat ccagcgctcg gccaccaagt
     3841 ccttgactgc gtattggacc gtccgcaaag aacgtccgat gagcttggaa agtgtcttct
     3901 ggctgaccac cacggcgttc tggtggccca tctgcgccac gaggtgatgc agcagcattg
     3961 ccgccgtggg tttcctcgca ataagcccgg cccacgcctc atgcgctttg cgttccgttt
     4021 gcacccagtg accgggcttg ttcttggctt gaatgccgat ttctctggac tgcgtggcca
     4081 tgcttatctc catgcggtag ggtgccgcac ggttgcggca ccatgcgcaa tcagctgcaa
     4141 cttttcggca gcgcgacaac aattatgcgt tgcgtaaaag tggcagtcaa ttacagattt
     4201 tctttaacct acgcaatgag ctattgcggg gggtgccgca atgagctgtt gcgtaccccc
     4261 cttttttaag ttgttgattt ttaagtcttt cgcatttcgc cctatatcta gttctttggt
     4321 gcccaaagaa gggcacccct gcggggttcc cccacgcctt cggcgcggct ccccctccgg
     4381 caaaaagtgg cccctccggg gcttgttgat cgactgcgcg gccttcggcc ttgcccaagg
     4441 tggcgctgcc cccttggaac ccccgcactc gccgccgtga ggctcggggg gcaggcgggc
     4501 gggcttcgcc ttcgactgcc cccactcgca taggcttggg tcgttccagg cgcgtcaagg
     4561 ccaagccgct gcgcggtcgc tgcgcgagcc ttgacccgcc ttccacttgg tgtccaaccg
     4621 gcaagcgaag cgcgcaggcc gcaggccgga ggcttttccc cagagaaaat taaaaaaatt
     4681 gatggggcaa ggccgcaggc cgcgcagttg gagccggtgg gtatgtggtc gaaggctggg
     4741 tagccggtgg gcaatccctg tggtcaagct cgtgggcagg cgcagcctgt ccatcagctt
     4801 gtccagcagg gttgtccacg ggccgagcga agcgagccag ccggtggccg ctcgcggcca
     4861 tcgtccacat atccacgggc tggcaaggga gcgcagcgac cgcgcagggc gaagcccgga
     4921 gagcaagccc gtagggcgcc gcagccgccg taggcggtca cgactttgcg aagcaaagtc
     4981 tagtgagtat actcaagcat tgagtggccc gccggaggca ccgccttgcg ctgcccccgt
     5041 cgagccggtt ggacaccaaa agggaggggc aggcatggcg gcatacgcga tcatgcgatg
     5101 caagaagctg gcgaaaatgg gcaacgtggc ggccagtctc aagcacgcct accgcgagcg
     5161 cgagacgccc aacgctgacg ccagcaggac gccagagaac gagcactggg cggccagcag
     5221 caccgatgaa gcgatgggcc gactgcgcga gttgctgcca gagaagcggc gcaaggacgc
     5281 tgtgttggcg gtcgagtacg tcatgacggc cagcccggaa tggtggaagt cggccagcca
     5341 agaacagcag gcggcgttct tcgagaaggc gcacaagtgg ctggcggaca agtacggggc
     5401 ggatcgcatc gtgacggcca gcatccaccg tgacgaaacc agcccgcaca tgaccgcgtt
     5461 cgtggtgccg ctgacgcagg acggcaggct gtcggccaag gagttcatcg gcaacaaagc
     5521 gcagatgacc cgcgaccaga ccacgtttgc ggccgctgtg gccgatctag ggctgcaacg
     5581 gggcatcgag ggcagcaagg cacgtcacac gcgcattcag gcgttctacg aggccctgga
     5641 gcggccacca gtgggccacg tcaccatcag cccgcaagcg gtcgagccac gcgcctatgc
     5701 accgcaggga ttggccgaaa agctgggaat ctcaaagcgc gttgagacgc cggaagccgt
     5761 ggccgaccgg ctgacaaaag cggttcggca ggggtatgag cctgccctac aggccgccgc
     5821 aggagcgcgt gagatgcgca agaaggccga tcaagcccaa gagacggccc gag
//
